Cell Atlas

Every cell is one point. Drag to pan, scroll to zoom, shift-drag to select a region; click any group in the legend to isolate it. Use Colour cells by to switch between cell type, cluster, library, quality-control metric and the expression of a single gene.

UMAP — this re-analysis

Drawn from the same exported coordinates and labels the interactive atlas above uses, so the figure and the atlas cannot disagree. 13,896 cells.

Coloured by cell type (25)
UMAP of the re-analysed SPIST_whole_adult, coloured by annotated cell type, with a legend naming each type

The legend in the panel names every type and its cell count. With 25 types, colour alone no longer separates them — read the legend, not the hue.

Coloured by Leiden cluster (48)
UMAP of the re-analysed SPIST_whole_adult, coloured by Leiden cluster, each labelled with its number

Each cluster's number is placed at its own centroid, so the grouping can be read without relying on colour. These are the clusters the cell-type annotation was built on.

Cell types in this dataset

Click a cell type to isolate it in the atlas above. The bar beside each percentage is drawn to scale: a cell type at 2.6 % fills 2.6 % of the track, so the rows can be ranked by eye without reading the numbers.

Cell type Cells Percent Markers
gastrodermis 4,614 marker genes
epidermis 2,894 marker genes
gastrodermis_alga_hosting 755 marker genes
gastrodermis_muscle 663 marker genes
neuron_Isl 652 marker genes
neuron_Atoh8 628 marker genes
calicoblast 584 marker genes
gastrodermis_like_2 436 marker genes
neuron_Pou4_2 340 marker genes
gland_2 284 marker genes
neurosecretory_progenitors 232 marker genes
digestive_filaments 214 marker genes
neuron_Pou4_Otp 206 marker genes
neuron_Pou4_1 190 marker genes
germline_oocytes_1 182 marker genes
neuron_Pou4_Gsx 162 marker genes
immune_3 142 marker genes
neuron_Isl_Nkx6 136 marker genes
immune_1 126 marker genes
gland_1_Xbp 121 marker genes
gland_3 117 marker genes
germline_oocytes_2 73 marker genes
immune_4 55 marker genes
immune_2 52 marker genes
cnidocyte 38 marker genes
25 cell types 13,896

Quality control for this dataset

These are the figures actually applied to the cells shown above, not the values requested in a configuration file. Every dataset is filtered independently; see Single-cell Data for the full table across all datasets.

Source BioProject PRJNA1223412
SRA study SRP563749
GEO series GSE289546
Library type 10x Genomics Chromium (high confidence)
Libraries integrated 1
Cells reported by source 15053
Cells before filtering 15,053
Cells after cell filtering 13,905
Doublets removed 9 (0.06%)
Cells after filtering 13,896
Retained 92.3%
Filtering strategy Per-library MAD outlier detection (adaptive)
Thresholds applied adaptive: per-library MAD (5.0 MADs) on UMI, genes and MT%, floor 200 genes, floor 500 UMI
Mitochondrial genes found 0 — too few for a reliable MT% filter, so none was applied
Doublet method Scrublet (per library)
Integration method none (single library)
Cell-type annotation Inherited from the source publication
Cell-type labels from GSE289546_Spis.cell_to_cts.csv.gz (100% of cells matched)
Pipeline version cnidosite-sc-1.0.0
Processed 2026-09-20T03:34:23+00:00
Clusters / cell types 48 / 25
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