Gene detail

Detailed information of ENSBQFP00000019284.1 in Heliopora coerulea

Genomic Location: JASJOG010000044.1:428163...458880
NR annotation: CAB3977525.1, C-1-tetrahydrofolate synthase, cytoplasmic [Paramuricea clavata]
Species Heliopora coerulea · all data for this species · gene families

Gene Structure

More details in JBrowse

Sequence

CDS

ENSBQFT00000021497

ATGGCTCAAATACTGAAAGGCTCCGAAGTCGCAAAAGATATTCGTGCTAAGTTAAAAGCTGTGGTTAATGAAACAAGGCAACAGTATCCAGAATTCAACCCTGGCTTGGCAATAGTGCAGATTGGAGATCGAGCTGATTCAACTTCTtacatcaaatcaaaatcaaaggCTGCTGTTGAGATTGGAATGATTGCTAGGCATGTCAAACTATCCAATAAAACAACTCAACAAGAAGTTCTGAAAAAAGTTGATGAACTTAATAAAGATCCCTCCATTCATGGTATTATTGTGCAGCTTCCTCCAGACTCCTGTGAACCCATTGATCCATCCATATGTACCAATGCTGTTGCACCAGAGAAAGATGTTGATGGTCTGAATGATGTTAACGCTGGAAGATTAGCAAGAGGTCAACTTAACGACTGTATCGTGCCTTGTACACCAAGAGGTTGCCTAGAACTtatcaaaagaactgGAATCGAAATTAGAGGAAAGAGAGCCATAGTTCTCGGACGGAGCAAAATTGTGGGTGCTCCAATGTCTGACTTGCTGACCTGGAATCACGCAACAGTTACAGTCTGTCATTCCAAAACGAAGGACTTACCTGATGTTGTTCGTGAGGGGGAGATAATCGTTGTTGGAATTGGCAGTCCCCACTTTGTGAAAGGTGACTGGATTAAAGAAGGAGCGGTTGTTATTGATTGTGGAATCAATGCTATCCCAGATTCTAGCAAAAAGAGCGGACATCGTTTGATTGGGGATGTGGATTATGCCGAGGGACTAAAAAGAGCGAGTTGGATAACCCCAGTACCTGGAGGTGTTGGTCCAATGACGGTTGCGATGTTGATGCAAAATACCGTAGAAAGTGCGaagaagatgattctgaaacaaaaGGCTGGTTTGTGGGAGATAAAGTTTTTACCATTGAATGTAAAACGTCCAGTTCCAAGTGATATCGATATTTCTAAAGCTCAAGATCCAAAGAGCATCCAACAATTAGCAAGTGAAATTGGCATCCGGGCGCACGAGCTGGACCTCTACGGgacaaagaaatgcaaagttTCCTTATCTGTGTTGGATCGTCTTCAGAATGAACCGAATGGAAAATATGTTGTTGTGACAGGAATTACACCAACACCTTTGGGTGAAGGGAAAAGTACGACGACCGTGGGTATTGCTCAAGCAATGGCTGCTCACTTGAAAAAGAACACATTCGCTTGTATTCGTCAACCTTCACAAGGACCAATTTTTGGAATTAAAGGTGGAGCAGCGGGTGGTGGTTATGCTCAGGTTATACCCATGGAAGAGTTCAATCTTCATCTTACGGGAGACATTCATGCCATTACTGCTGCCAACAATCTTATCGCTGCTGCGATAGACACGAGAATATTCCACGAATCTACCCAAACGAACGAGgcGTTGTTTAACAGACTCGTGCCAGTTCAAGATGGCAAAAGGACATTTTCGGAAATAATGTTGAGAAGATTGAAGAAACTTGGCATAAACAAAACGGAACCAGATGAACTGACCCCGGATGAAGCGGCAGCATTCGCAAGATTGGATATCGATCCTGAGAGTATTACTTGGCAAAGAGTGGTAGACACAAACGATAGGTTCCTAAGGAAGATCACCATTGGACAGTCTCCTACTGAGAAAGGAATGAATCGAGAGTGTCAATTTGATATCTCGgttgcaagtgaaattatggccGTCTTGGCACTTGCGAAAAATCTTAAGGACATGAAAGAAAGGTTTGGAAAGATGGTGGTAGCGTGTGACAAGAAAGGACAACCAGTGACTGCTGATGACCTGgGATTAACTGGTGCTCTGGCAGTCTTGATGAAAGACACGATTAAGCCAAACTTGATGCAAACTTTGGAGGGCTCCCCAGTCTTCGTGCATGCAGGACCCTTCGCAAATATTGCGCACGGGAATTCGTCGATCATCGCCGACATAATTGCGCTGAAGTTAGTTGGGAAGGAAGGCTTCGTTTTCACTGAGGCTGGCTTTGGGGCAGATATTGGAATggagaaattctttaacattaaGTGCAGGTATAGCGGTTTGATCCCTAACTGTGTTGTTCTCGTAGCTACGGTCCGAGCGATAAAAATGCATGGAGGAGGTCCAAAGGTGGTTGCTGGCCGTCCACTGGCGCAGGTCTACGTTGAAGAGAACTTAGAACTGGTGAAAAATGGATTTGTTAATCTTGCGAAGCAGATCGAGAACGCTATGTACTTCGGAGTTCCTGTAGTGGTGGCTATTAACCGTTTCAAATCTGACACGGATGCAGAGGTTCAACTTCTTATAGATTTAAGCAAGGACGCTGGAGCGTTTGACGCTGTCGCTTCAACACATTGGGCAGATGGAGGCTCTGGAGCTGTTAAACTGGCAGAGGTAGTGTTAAAGGCCACGGACCAGCCTCTGGACTTCAAGTTCCTTTACGACCTCGAGCTGCTGGTCGAAAAGAAGATAGAAATCATCGCTCAGAAGATCTATGGCGCGGATGGCGTGGAGTTTTCAGACGAAGCTCAAAGGAAAATTAATCTTTTTAGGGATCAGggTTTCAACGATCTTCCTATCTGTATGGCTAAAACACATTTGTCCTTGTCACATAACCAAGAGTTGAAAGGAGCTCCAACAGGATTTGTCCTACCCATTCGAGATGTACGGGCAAGTGTTGGAGCAGGTTTCTTATTTCCTCTAGTCGGCACCATGAGCACAATGCCAGGGCTTCCCACAAGACCTTGCTTCTACGATGTAGACTTGGACACCGAGACCGAGGAAGTACTGGGACTCTTTTAG

Protein

UniProt (Swiss-Prot top hit)

UniProt accession	Description
P11586	C-1-tetrahydrofolate synthase, cytoplasmic OS=Homo sapiens OX=9606 GN=MTHFD1 PE=1 SV=4
Q5R8P0	C-1-tetrahydrofolate synthase, cytoplasmic OS=Pongo abelii OX=9601 GN=MTHFD1 PE=2 SV=3
Q922D8	C-1-tetrahydrofolate synthase, cytoplasmic OS=Mus musculus OX=10090 GN=Mthfd1 PE=1 SV=4

Gene family

Family type	Membership / link
Orthogroup (gene family)	OG0001676 (this species only) · gene tree & orthology

Pfam domain

Pfam accession	Pfam name	Description	Type	Source
PF02882 all species →	THF_DHG_CYH_C	Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain	Domain	Interproscan
PF01268 all species →	FTHFS	Formate--tetrahydrofolate ligase	Family	Interproscan
PF00763 all species →	THF_DHG_CYH	Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain	Domain	Interproscan

InterPro

InterPro term	Type	Description	Source
IPR036291 all species →	Homologous_superfamily	NAD(P)-binding domain superfamily	Interproscan
IPR020631 all species →	Domain	Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain	Interproscan
IPR000559 all species →	Family	Formate-tetrahydrofolate ligase, FTHFS	Interproscan
IPR046346 all species →	Homologous_superfamily	Aminoacid dehydrogenase-like, N-terminal domain superfamily	Interproscan
IPR020867 all species →	Conserved_site	Tetrahydrofolate dehydrogenase/cyclohydrolase, conserved site	Interproscan
IPR020630 all species →	Domain	Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain	Interproscan
IPR000672 all species →	Family	Tetrahydrofolate dehydrogenase/cyclohydrolase	Interproscan
IPR020628 all species →	Conserved_site	Formate-tetrahydrofolate ligase, FTHFS, conserved site	Interproscan
IPR027417 all species →	Homologous_superfamily	P-loop containing nucleoside triphosphate hydrolase	Interproscan

PANTHER

PANTHER term	Description	Source
PTHR48099 all species →	C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED	Interproscan

Gene Ontology

GO term	Category	Description	Source
GO:0004488 all species →	Molecular Function	methylenetetrahydrofolate dehydrogenase (NADP+) activity	Interproscan
GO:0004329 all species →	Molecular Function	formate-tetrahydrofolate ligase activity	Interproscan
GO:0005524 all species →	Molecular Function	ATP binding	Interproscan
GO:0004477 all species →	Molecular Function	methenyltetrahydrofolate cyclohydrolase activity	Interproscan
GO:0005829 all species →	Cellular Component	cytosol	Interproscan
GO:0035999 all species →	Biological Process	tetrahydrofolate interconversion	Interproscan
GO:0003824 all species →	Molecular Function	catalytic activity	Interproscan

Search by domain instead of by gene. Any accession above (InterPro, Pfam, PANTHER, GO, KEGG) can be used as a query on the Functional Domain Search page, which searches all 148 annotated genomes at once.

KEGG pathway

KO	Enzyme	Enzyme ID	Pathway	Map ID	Source
K00288	MTHFD; methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase / formyltetrahydrofolate synthetase	EC:1.5.1.5 EC:3.5.4.9 EC:6.3.4.3	Exosome	ko04147	deepkoala

Searching by KO or pathway ID across all species is available on the KEGG Pathway page.

Expression pattern (RNA-seq)

Transcript abundance of ENSBQFP00000019284.1 across 37 RNA-seq samples of Heliopora coerulea. Values are TPM (transcripts per million) from the StringTie quantification; one bar is one sample, grouped and coloured by condition, sorted by expression within each group.

37Samples

34TPM > 0

6Conditions

188.2Max TPM

71.3Mean TPM

By condition

Condition	Samples	TPM > 0	Mean TPM	Max TPM
Whole coral	17	17	44.00	60.61
polyp and skeleton · 31C, 3week	6	6	107.33	152.15
polyp and skeleton · 28C, 3week	6	4	72.00	141.82
polyp and skeleton · 26C, 3week	4	4	99.40	139.12
polyp and skeleton · 28C, 24hr	2	2	113.41	121.98
polyp and skeleton · 31C, 24hr	2	1	94.09	188.19

Per sample · hover a bar for the full sample record

Show the sample table (37 samples)

SRA run	Condition	Tissue	Developmental stage	Treatment	Study	TPM
ERR6178777	Whole coral	Whole coral	not recorded	not recorded	ERP120267	60.61
ERR6178775	Whole coral	Whole coral	not recorded	not recorded	ERP120267	53.37
ERR6178780	Whole coral	Whole coral	not recorded	not recorded	ERP120267	49.57
ERR6178773	Whole coral	Whole coral	not recorded	not recorded	ERP120267	49.29
ERR6178387	Whole coral	Whole coral	not recorded	not recorded	ERP120267	49.02
ERR6178778	Whole coral	Whole coral	not recorded	not recorded	ERP120267	48.52
ERR6178770	Whole coral	Whole coral	not recorded	not recorded	ERP120267	46.97
ERR6178774	Whole coral	Whole coral	not recorded	not recorded	ERP120267	45.27
ERR6178776	Whole coral	Whole coral	not recorded	not recorded	ERP120267	44.30
ERR6178781	Whole coral	Whole coral	not recorded	not recorded	ERP120267	40.82
ERR6178779	Whole coral	Whole coral	not recorded	not recorded	ERP120267	39.07
ERR6178389	Whole coral	Whole coral	not recorded	not recorded	ERP120267	39.04
ERR6178771	Whole coral	Whole coral	not recorded	not recorded	ERP120267	38.39
ERR6178388	Whole coral	Whole coral	not recorded	not recorded	ERP120267	36.85
ERR6178772	Whole coral	Whole coral	not recorded	not recorded	ERP120267	36.53
ERR6178783	Whole coral	Whole coral	not recorded	not recorded	ERP120267	36.03
ERR6178782	Whole coral	Whole coral	not recorded	not recorded	ERP120267	34.33
SRR12587804	polyp and skeleton · 31C, 3week	polyp and skeleton	not recorded	31C, 3week	SRP115860	152.15
SRR12578065	polyp and skeleton · 31C, 3week	polyp and skeleton	not recorded	31C, 3week	SRP115860	126.57
SRR12578068	polyp and skeleton · 31C, 3week	polyp and skeleton	not recorded	31C, 3week	SRP115860	111.37
SRR12587799	polyp and skeleton · 31C, 3week	polyp and skeleton	not recorded	31C, 3week	SRP115860	108.51
SRR12578066	polyp and skeleton · 31C, 3week	polyp and skeleton	not recorded	31C, 3week	SRP115860	85.22
SRR12587800	polyp and skeleton · 31C, 3week	polyp and skeleton	not recorded	31C, 3week	SRP115860	60.17
SRR12587802	polyp and skeleton · 28C, 3week	polyp and skeleton	not recorded	28C, 3week	SRP115860	141.82
SRR12587803	polyp and skeleton · 28C, 3week	polyp and skeleton	not recorded	28C, 3week	SRP115860	116.57
SRR12587808	polyp and skeleton · 28C, 3week	polyp and skeleton	not recorded	28C, 3week	SRP115860	92.26
SRR12587807	polyp and skeleton · 28C, 3week	polyp and skeleton	not recorded	28C, 3week	SRP115860	81.35
SRR12578067	polyp and skeleton · 28C, 3week	polyp and skeleton	not recorded	28C, 3week	SRP115860	0.00
SRR5949849	polyp and skeleton · 28C, 3week	polyp and skeleton	not recorded	28C, 3week	SRP115860	0.00
SRR12587801	polyp and skeleton · 26C, 3week	polyp and skeleton	not recorded	26C, 3week	SRP115860	139.12
SRR12587806	polyp and skeleton · 26C, 3week	polyp and skeleton	not recorded	26C, 3week	SRP115860	126.74
SRR12587798	polyp and skeleton · 26C, 3week	polyp and skeleton	not recorded	26C, 3week	SRP115860	66.80
SRR12587805	polyp and skeleton · 26C, 3week	polyp and skeleton	not recorded	26C, 3week	SRP115860	64.94
SRR5949850	polyp and skeleton · 28C, 24hr	polyp and skeleton	not recorded	28C, 24hr	SRP115860	121.98
SRR12578063	polyp and skeleton · 28C, 24hr	polyp and skeleton	not recorded	28C, 24hr	SRP115860	104.83
SRR12578064	polyp and skeleton · 31C, 24hr	polyp and skeleton	not recorded	31C, 24hr	SRP115860	188.19
SRR5949848	polyp and skeleton · 31C, 24hr	polyp and skeleton	not recorded	31C, 24hr	SRP115860	0.00

Source: CnidoSite RNA-seq expression matrices (HCOER_TPM, StringTie quantification over 37 runs), joined to SRA sample metadata. Samples whose tissue/treatment is not recorded in the source metadata are grouped by the descriptor carried in the expression matrix itself.

Co-expression network

Genes whose expression across the transcriptome samples of Heliopora coerulea tracks this one. Counts are over the whole network; the network view itself draws at most 100 partners per query gene (CNIDO_NET_TOP_K), so a hub gene can show fewer edges than the number below.

Direction	Partners	Most correlated partner	PCC
Positively correlated	30	ENSBQFP00000000265	0.897113499176275
Negatively correlated	16	ENSBQFP00000003258	-0.787448417761714

Open this gene in Network Analysis → The network opens with the gene already entered and both directions selected. There you can add up to 9 more genes, switch between the positive and negative network, and export the edge list. Partner counts were matched on ENSBQFP00000019284, the spelling this network uses.

Single-cell expression

Whether this gene can be visualised in the single-cell atlases of Heliopora coerulea, and in which cell types it is a marker. Expression is stored per cell, so the violin plot and the cell-type means are computed in the viewer — open a dataset to see them.

No single-cell dataset has been published for this species. The atlases that do exist are listed in the Cell Atlas.

Epigenetic marks

Chromatin and DNA-methylation data covering this gene. Peak calls are listed per sample with the genomic region the peak falls in; DNA methylation is listed as sample availability only, because those tables are queried by the DNA Methylation page itself.

No epigenomic data has been deposited for this species. The assays that exist across the site are described in the Epigenomic Data module.

What you can do with this gene

Every tool below opens with this gene already entered, so you land on the analysis rather than on an empty form. Links open in a new tab.

Analysis	What it does	Status
Primer design	Design PCR / qPCR primers on this gene’s sequence with the published primer3 settings. Opens with the primers already computed.	template found	open →
BLAST	Search this gene’s sequence against the CnidoSite BLAST databases. The query is filled in and the species’ database is preselected — press Run to start.	template found	open →
Expression heatmap	Draw this gene’s expression as a heatmap across all RNA-seq samples, and compare it with the genes you add next to it.	ready	open →
Gene family / orthogroup	Look this gene up in the single-copy orthogroups built from the high-quality cnidarian genomes, and see which other species carry an orthologue.		open →
Gene set analysis	Start a gene-set enrichment analysis with this gene as the seed list. Add more genes on that page before running it.		open →