Gene detail

Detailed information of ENSBQFP00000030381.1 in Heliopora coerulea

Genomic Location: JASJOG010000103.1:335388...379206
NR annotation: XP_028392901.1, bifunctional glutamate/proline--tRNA ligase-like [Dendronephthya gigantea]
Species Heliopora coerulea · all data for this species · gene families

Gene Structure

More details in JBrowse

Sequence

CDS

ENSBQFT00000034081

ATGAGGTTACGTTCGGTGGTTGCAATTTACCGGTGCCAACTTCAAAGAAGGTTAACATTTCATTCCACATTTGAGGCAAAGAGACGAATATTAAACTTACCGCGTCGTTCATTTATTAGCAGTTTCGATATGCAAACCCATAAACTCACCGCGAATTCTTCTCAACCACCTTTTGCCTCCTTATTGGTTGTAGACTATATCAAAAGCAAATATAAAGTTGACGTGGACTGGAAAAATGCAACCTCATTAGAGATCAATGGAAATGTTGCTCTAACTTGTGATCACAGTATTGCTCGCTACTTGGCCAGGATTGGTGATGAATTTAAGCTTTATGGCAATAACATTTTGCAGGCAACAGAGGTTGACAGTTGGATGGACTTTAGTGCAACATTCTTGAAGGATTCCAAGAAATTCCAGTCTGCTCTTCACCACTTAGACAAACAATTATCACCAGTCACTTACTTGGTGGGGAAGACAATAAGTCTAGCAGACTTTGCAGTCTGGGGGGCATTGAAAGCTAGTGACAGGTGGGAGAACTTGTTAAAGAACGGAGAGGCTAGAAATGTGTCAAGGTGGTACAATTTTCTTTCCTCTCAAAACAGTTTTAAGCAGTATTGTGGCCAGGCAACCTCATCAGATAAACCACAGAATGAAATTAAAACCCAGGGCAAGTTCATTGAATTGGAAGGTGCGGTAGAGGGCAAAGTGGTAGTTAGATTTCCGCCTGAAGCAAGTGGGTATTTGCACATCGGACATGCTAAGGCAGCCTTACTGAACCAATATTACAGAGATCACTACAAAGGTGAAATGATCATGAGGTTTGATGACACAAATCCCGCCAAAGAGAATGCTGAGTTTGAACAGGTTATATTAGAGGACTTGACATTGTTGAAAGTGAAGCCAGATAGGTTTACCAGATCTTCAGATCATTTTGATAAAATGGTGAAACTTGCAGAGAAGATGATCAAGGATGGCAATGCTTATGTAGACGACACTCCAGCTGAGAAAATGAgggaagaaagagaaaaaagaactaAATCAACAAATCGTGATAACTcggtggaaaagaacattcaGATGTgggaagaaatgaaaaaaggaaGTCCTTACGGACAGACTTGCGCTCTTCGTGCAAAGATTGACTACGAGTCAGACAATGGATGTTTACGGGATCCTACACTTTATCGATGTAAACCTGAAACACATGTCCAAACTGGTGATAGATACAAAGTGTATCCAACATATGATTTCGCCTGTCCTATAGTGGATAGTGTTGAAGGCGTGACACATGCTTTAAGAACTACAGAGTATCATGATCGCGATCCTCAGTATTTTTGGATCATTGAGGCACTTGGTTTACGCAAGCCCACGATCATGGAGTTCAGCAAGATTAATTTGCAAAATACAGTCTTATCAAAAAGGAAACTTACTTGGCTTGTGCAGGAGAAAGTTGTTGATGGCTGGGATGACCCGAGGTTTCCGACTGTCCGAGGAGTTCTGAGAAGAGGAATGACTGTAAAGGGACTTTTAGAATTTATCTTGCTACAGGGATTTTCCAAATCTAACGTTCACATGGAATGGGATAAAATTTGGGCCATCAATAGAAAGGTAATTGATCCAATCTCGCCTCGTTACACTGCTTTGAAAAAAGACGAGAAAGTTATGGTCAATATTCCTGGAATTGCTGAAGAGTGTGAAGATGCCTTTCTTAATCCTAAGAATGCAGAAATGGGTAAGAAGAAAGTTTGGAGGTCCTCTAAAGTGTACATTGAAGGAGCAGATGCGACAACATTGTCCTCTGGCGAAAAAATAACCTTGATTAACTGGGGTAACTTCATCGTCAAAGCAATTAACAGGAATTCTTCTGGCGCAATCACGTCCATAGACGTTGAGCCAAATCTGGAAGATACGGATTATAAAAATACTGCCAAACTGACATGGCTGGCTATTACTGAGGAAGCGCCATTGCTGCCAACGTTAGTGGTCCACTTTGACCACATTGTATCCAAAGGCATCCTGACGAAGAATGACGACTTTAAAAACTATGTCAATAAAGACACCAAGAGCGAGATGGAAATGCTTGGAGATCCAGGCTTAGCAAACGTCAAGAAAGGGGACATAATTCAGTTGCAAAGACGAGGGTTTTTCATATGTGACCAGCCGTACGGCGAACCAAGTCGTTACACTGGAAAATCCACTCCTTGTATCCTGTTTTCAATCCCAGACGGCCATACAAAGGCCATGCCAACCTCAGGTtccaaatcaaaaacaaaacaaaataaagagaAGGGTGAtcaaaagaaagataaaaagaatGCGAGCGAACCTCCGGTCAAGGCGGGAAACCCTGTAGTTCTAGAAACATCGCCTGAAGTTGAGGATTTGGTGAAGAAAATCGGCGAGCAAGGTGATAAAGTTCGACAGTTGAAGAGCGACGGTGCGGATAAGGCTGAAGTCACAAAAGAAGTGAATGTTCTACTCGATCTAAAAGGTCAGTACAAGACGCTGACAGGACAAGATTTTAAACCGGCTAACAACGgcaagaaagagaagaagaaggaaaagacgAAAGCGACCAAGGCAGAACAAGCCGTTAAGAAGGAAGAACAGAAACCAAGCCAAGATGAAAGGACAGTGAAAAAGGTTACAAGACTTGGACTTGAAGCAAAGAAAGAAGAGAATCTTCCTGACTGGTATTCTCAGATCATCACGAAGGCAGAATTGATCGAGTACTATGACGTGAGTGGATGTTATATCCTGCGTCCCTGGTCGTATGCAATATGGGAAAGTATTAAAGCATTCTTTGACagtgaaattaataaacttggTGTCGAGAATTGTTATTTTCCAATCTTCGTTTCTCAGTCTGCTTTGGAGCGTGAAAAGACACATATCGCTGACTTCGCGCCTGAGGTTGCTTGGGTTACAAAATCAGGACAGTCTGAGCTCATGGAGCCCATAGCCATACGTCCAACCAGTGAAACAGTAATGTATCCGGCTTATGCGAAGTGGATCAAGTCTCACCGAGATTTGCCTCTAAAATTGAACCAGTGGAACAACGTTGTGAGATGGGAGTTCAAACATCCGCAGCCTTTCTTGAGAACGAGAGAATTTTTATGGCAAGAAGGTCACACGGCATTTGCCGACTATGATGAAGCTGTACAAGAGGTGTATGCCATACTAGATCTTTATGCTCGGGTGTACACAGATCTCTTGGCAATACCTGTCGTCAAAGGAcgaaaaactgaaaaagaaaagtttgcTGGTGGTGACTTTACTACCACTGTGGAGGTTTATATCTCTGCCAGTGGCCGGGGTATTCAAGGTGCCACCTCACATCACTTGGGACAAAACTTCTCCAAGATGTTTGACATTGTTTTTGAAGATCCTGACAAGCCCGGGGAAAAGAGGTATGTTTATCAGAATTCCTGGGGTATAACAACTCGTACTATTGGTGTGATGTGTATGGTACATGGAGATAACAAGGGACTTGTCCTGCCACCTCGTATTGCAGCCGTTCAGGTCGTGATCATTCCTTGTGGACTCACAGCATCCTTGAAAGAAGATGTGAAACAGAGCTTGCTCGCCTTCTGTAGAGATTTTCAAGGCACTCTCCAGCAAAATGGAGTCCGCGCCAAACTTGATGATCGGGATAATTATTCGCCAGGATGGAAATTTAATCATTGGGAACTAAAGGGGGTCCCAATCCGTGTCGAACTTGGTCCTCGAGATGTGAAGAACAATCAGTTTGTCAGCGTGCGGCGGGACACTGGTGAGAAGGTGACTTTGAAGGCTGAAGATCATGTTTCCCAAATTAAGGAAATACTCGATGAAATGCACAATGACCTATACAAAAAGGCGCAAAAGGAACTGGATGAAAATATGACGGTTACAGCGGACTGGAAACATTTGTGTACGATCTTAGACCAACGTAAGATTATCCAAGCCCCATTCTGTGGTGAAATAAAGTGCGAGGATAAGATTAAGAAAGACAGTGCAAGGGATGCTGTTGTTGAAGAAGGAGCACCAGCTATGGGAGCCAAGGCGCTGTGTATTCCGTTCAATCAACCACGCGAAGTCACTTCGGAAACGAAGTGCATTCACCCAGAATGCGGCAAGCCAGCCAAATACTATACCCTCTTTGGACGCAGTTATTAG

Protein

UniProt (Swiss-Prot top hit)

UniProt accession	Description
P07814	Bifunctional glutamate/proline--tRNA ligase OS=Homo sapiens OX=9606 GN=EPRS1 PE=1 SV=5
Q7SIA2	Bifunctional glutamate/proline--tRNA ligase OS=Cricetulus griseus OX=10029 GN=EPRS1 PE=1 SV=3
Q8CGC7	Bifunctional glutamate/proline--tRNA ligase OS=Mus musculus OX=10090 GN=Eprs1 PE=1 SV=4

Gene family

Family type	Membership / link
Orthogroup (gene family)	OG0003095 (this species only) · gene tree & orthology

Pfam domain

Pfam accession	Pfam name	Description	Type	Source
PF03950 all species →	tRNA-synt_1c_C	tRNA synthetases class I (E and Q), anti-codon binding domain	Domain	Interproscan
PF03129 all species →	HGTP_anticodon	Anticodon binding domain	Domain	Interproscan
PF00749 all species →	tRNA-synt_1c	tRNA synthetases class I (E and Q), catalytic domain	Domain	Interproscan
PF00587 all species →	tRNA-synt_2b	tRNA synthetase class II core domain (G, H, P, S and T)	Domain	Interproscan
PF20974 all species →	tRNA-synt_1c_C2	tRNA synthetases class I (E and Q), anti-codon binding domain	Domain	Interproscan
PF09180 all species →	ProRS-C_1	Prolyl-tRNA synthetase, C-terminal	Domain	Interproscan
PF00458 all species →	WHEP-TRS	WHEP-TRS domain	Domain	Interproscan

InterPro

InterPro term	Type	Description	Source
IPR020059 all species →	Domain	Glutamyl/glutaminyl-tRNA synthetase, class Ib, anti-codon binding domain	Interproscan
IPR006195 all species →	Domain	Aminoacyl-tRNA synthetase, class II	Interproscan
IPR001412 all species →	Conserved_site	Aminoacyl-tRNA synthetase, class I, conserved site	Interproscan
IPR016061 all species →	Domain	Proline-tRNA ligase, class II, C-terminal	Interproscan
IPR004154 all species →	Domain	Anticodon-binding	Interproscan
IPR017449 all species →	Homologous_superfamily	Prolyl-tRNA synthetase, class II	Interproscan
IPR036282 all species →	Homologous_superfamily	Glutathione S-transferase, C-terminal domain superfamily	Interproscan
IPR004499 all species →	Family	Proline-tRNA ligase, class IIa, archaeal-type	Interproscan
IPR033721 all species →	Domain	Prolyl-tRNA synthetase, catalytic domain	Interproscan
IPR045864 all species →	Homologous_superfamily	Class II Aminoacyl-tRNA synthetase/Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL)	Interproscan
IPR004526 all species →	Family	Glutamyl-tRNA synthetase, archaeal/eukaryotic cytosolic	Interproscan
IPR020058 all species →	Domain	Glutamyl/glutaminyl-tRNA synthetase, class Ib, catalytic domain	Interproscan
IPR000738 all species →	Domain	WHEP-TRS domain	Interproscan
IPR020061 all species →	Homologous_superfamily	Glutamine-tRNA ligase, alpha-bundle domain superfamily	Interproscan
IPR014729 all species →	Homologous_superfamily	Rossmann-like alpha/beta/alpha sandwich fold	Interproscan
IPR011035 all species →	Homologous_superfamily	Large ribosomal subunit protein bL25/Gln-tRNA synthetase, anti-codon-binding domain superfamily	Interproscan
IPR002314 all species →	Domain	Aminoacyl-tRNA synthetase, class II (G/ P/ S/T)	Interproscan
IPR049437 all species →	Domain	tRNA synthetases class I (E and Q), anti-codon binding domain	Interproscan
IPR010987 all species →	Domain	Glutathione S-transferase, C-terminal-like	Interproscan
IPR000924 all species →	Family	Glutamyl/glutaminyl-tRNA synthetase	Interproscan
IPR020056 all species →	Homologous_superfamily	Large ribosomal subunit protein bL25/Gln-tRNA synthetase, N-terminal	Interproscan
IPR009068 all species →	Homologous_superfamily	uS15/NS1, RNA-binding domain superfamily	Interproscan
IPR036621 all species →	Homologous_superfamily	Anticodon-binding domain superfamily	Interproscan

PANTHER

PANTHER term	Description	Source
PTHR43382 all species →	PROLYL-TRNA SYNTHETASE	Interproscan

Gene Ontology

GO term	Category	Description	Source
GO:0000166 all species →	Molecular Function	nucleotide binding	Interproscan
GO:0004812 all species →	Molecular Function	aminoacyl-tRNA ligase activity	Interproscan
GO:0005524 all species →	Molecular Function	ATP binding	Interproscan
GO:0005737 all species →	Cellular Component	cytoplasm	Interproscan
GO:0006418 all species →	Biological Process	tRNA aminoacylation for protein translation	Interproscan
GO:0004827 all species →	Molecular Function	proline-tRNA ligase activity	Interproscan
GO:0006433 all species →	Biological Process	prolyl-tRNA aminoacylation	Interproscan
GO:0017101 all species →	Cellular Component	aminoacyl-tRNA synthetase multienzyme complex	Interproscan
GO:0004818 all species →	Molecular Function	glutamate-tRNA ligase activity	Interproscan
GO:0006424 all species →	Biological Process	glutamyl-tRNA aminoacylation	Interproscan
GO:0043039 all species →	Biological Process	tRNA aminoacylation	Interproscan
GO:0006412 all species →	Biological Process	translation	Interproscan

Search by domain instead of by gene. Any accession above (InterPro, Pfam, PANTHER, GO, KEGG) can be used as a query on the Functional Domain Search page, which searches all 148 annotated genomes at once.

KEGG pathway

KO	Enzyme	Enzyme ID	Pathway	Map ID	Source
K14163	EPRS; bifunctional glutamyl/prolyl-tRNA synthetase	EC:6.1.1.17 EC:6.1.1.15	Transfer RNA biogenesis	ko03016	deepkoala

Searching by KO or pathway ID across all species is available on the KEGG Pathway page.

Expression pattern (RNA-seq)

Transcript abundance of ENSBQFP00000030381.1 across 37 RNA-seq samples of Heliopora coerulea. Values are TPM (transcripts per million) from the StringTie quantification; one bar is one sample, grouped and coloured by condition, sorted by expression within each group.

37Samples

36TPM > 0

6Conditions

223.6Max TPM

63.8Mean TPM

By condition

Condition	Samples	TPM > 0	Mean TPM	Max TPM
Whole coral	17	17	46.47	60.56
polyp and skeleton · 31C, 3week	6	6	57.67	82.55
polyp and skeleton · 28C, 3week	6	6	112.31	223.57
polyp and skeleton · 26C, 3week	4	3	49.23	68.23
polyp and skeleton · 28C, 24hr	2	2	83.88	86.80
polyp and skeleton · 31C, 24hr	2	2	92.68	110.91

Per sample · hover a bar for the full sample record

Show the sample table (37 samples)

SRA run	Condition	Tissue	Developmental stage	Treatment	Study	TPM
ERR6178776	Whole coral	Whole coral	not recorded	not recorded	ERP120267	60.56
ERR6178771	Whole coral	Whole coral	not recorded	not recorded	ERP120267	58.42
ERR6178387	Whole coral	Whole coral	not recorded	not recorded	ERP120267	55.89
ERR6178778	Whole coral	Whole coral	not recorded	not recorded	ERP120267	53.37
ERR6178781	Whole coral	Whole coral	not recorded	not recorded	ERP120267	53.25
ERR6178389	Whole coral	Whole coral	not recorded	not recorded	ERP120267	53.01
ERR6178773	Whole coral	Whole coral	not recorded	not recorded	ERP120267	48.35
ERR6178774	Whole coral	Whole coral	not recorded	not recorded	ERP120267	48.20
ERR6178779	Whole coral	Whole coral	not recorded	not recorded	ERP120267	47.61
ERR6178782	Whole coral	Whole coral	not recorded	not recorded	ERP120267	47.41
ERR6178780	Whole coral	Whole coral	not recorded	not recorded	ERP120267	43.55
ERR6178783	Whole coral	Whole coral	not recorded	not recorded	ERP120267	42.59
ERR6178770	Whole coral	Whole coral	not recorded	not recorded	ERP120267	40.16
ERR6178388	Whole coral	Whole coral	not recorded	not recorded	ERP120267	40.09
ERR6178777	Whole coral	Whole coral	not recorded	not recorded	ERP120267	38.81
ERR6178772	Whole coral	Whole coral	not recorded	not recorded	ERP120267	38.11
ERR6178775	Whole coral	Whole coral	not recorded	not recorded	ERP120267	20.62
SRR12587800	polyp and skeleton · 31C, 3week	polyp and skeleton	not recorded	31C, 3week	SRP115860	82.55
SRR12578066	polyp and skeleton · 31C, 3week	polyp and skeleton	not recorded	31C, 3week	SRP115860	72.74
SRR12578065	polyp and skeleton · 31C, 3week	polyp and skeleton	not recorded	31C, 3week	SRP115860	65.23
SRR12587799	polyp and skeleton · 31C, 3week	polyp and skeleton	not recorded	31C, 3week	SRP115860	50.52
SRR12587804	polyp and skeleton · 31C, 3week	polyp and skeleton	not recorded	31C, 3week	SRP115860	37.95
SRR12578068	polyp and skeleton · 31C, 3week	polyp and skeleton	not recorded	31C, 3week	SRP115860	37.01
SRR12578067	polyp and skeleton · 28C, 3week	polyp and skeleton	not recorded	28C, 3week	SRP115860	223.57
SRR5949849	polyp and skeleton · 28C, 3week	polyp and skeleton	not recorded	28C, 3week	SRP115860	141.98
SRR12587802	polyp and skeleton · 28C, 3week	polyp and skeleton	not recorded	28C, 3week	SRP115860	107.01
SRR12587807	polyp and skeleton · 28C, 3week	polyp and skeleton	not recorded	28C, 3week	SRP115860	88.71
SRR12587808	polyp and skeleton · 28C, 3week	polyp and skeleton	not recorded	28C, 3week	SRP115860	57.87
SRR12587803	polyp and skeleton · 28C, 3week	polyp and skeleton	not recorded	28C, 3week	SRP115860	54.72
SRR12587805	polyp and skeleton · 26C, 3week	polyp and skeleton	not recorded	26C, 3week	SRP115860	68.23
SRR12587806	polyp and skeleton · 26C, 3week	polyp and skeleton	not recorded	26C, 3week	SRP115860	67.19
SRR12587798	polyp and skeleton · 26C, 3week	polyp and skeleton	not recorded	26C, 3week	SRP115860	61.51
SRR12587801	polyp and skeleton · 26C, 3week	polyp and skeleton	not recorded	26C, 3week	SRP115860	0.00
SRR5949850	polyp and skeleton · 28C, 24hr	polyp and skeleton	not recorded	28C, 24hr	SRP115860	86.80
SRR12578063	polyp and skeleton · 28C, 24hr	polyp and skeleton	not recorded	28C, 24hr	SRP115860	80.97
SRR5949848	polyp and skeleton · 31C, 24hr	polyp and skeleton	not recorded	31C, 24hr	SRP115860	110.91
SRR12578064	polyp and skeleton · 31C, 24hr	polyp and skeleton	not recorded	31C, 24hr	SRP115860	74.45

Source: CnidoSite RNA-seq expression matrices (HCOER_TPM, StringTie quantification over 37 runs), joined to SRA sample metadata. Samples whose tissue/treatment is not recorded in the source metadata are grouped by the descriptor carried in the expression matrix itself.

Co-expression network

Genes whose expression across the transcriptome samples of Heliopora coerulea tracks this one. Counts are over the whole network; the network view itself draws at most 100 partners per query gene (CNIDO_NET_TOP_K), so a hub gene can show fewer edges than the number below.

Direction	Partners	Most correlated partner	PCC
Positively correlated	21	ENSBQFP00000046514	0.912124633786703
Negatively correlated	42	ENSBQFP00000012323	-0.775445940871556

Open this gene in Network Analysis → The network opens with the gene already entered and both directions selected. There you can add up to 9 more genes, switch between the positive and negative network, and export the edge list. Partner counts were matched on ENSBQFP00000030381, the spelling this network uses.

Single-cell expression

Whether this gene can be visualised in the single-cell atlases of Heliopora coerulea, and in which cell types it is a marker. Expression is stored per cell, so the violin plot and the cell-type means are computed in the viewer — open a dataset to see them.

No single-cell dataset has been published for this species. The atlases that do exist are listed in the Cell Atlas.

Epigenetic marks

Chromatin and DNA-methylation data covering this gene. Peak calls are listed per sample with the genomic region the peak falls in; DNA methylation is listed as sample availability only, because those tables are queried by the DNA Methylation page itself.

No epigenomic data has been deposited for this species. The assays that exist across the site are described in the Epigenomic Data module.

What you can do with this gene

Every tool below opens with this gene already entered, so you land on the analysis rather than on an empty form. Links open in a new tab.

Analysis	What it does	Status
Primer design	Design PCR / qPCR primers on this gene’s sequence with the published primer3 settings. Opens with the primers already computed.	template found	open →
BLAST	Search this gene’s sequence against the CnidoSite BLAST databases. The query is filled in and the species’ database is preselected — press Run to start.	template found	open →
Expression heatmap	Draw this gene’s expression as a heatmap across all RNA-seq samples, and compare it with the genes you add next to it.	ready	open →
Gene family / orthogroup	Look this gene up in the single-copy orthogroups built from the high-quality cnidarian genomes, and see which other species carry an orthologue.		open →
Gene set analysis	Start a gene-set enrichment analysis with this gene as the seed list. Add more genes on that page before running it.		open →