Gene detail

Detailed information of ENSBQFP00000032001 in Heliopora coerulea

Genomic Location: JASJOG010000095.1:680111...685767
NR annotation: XP_028407939.1, twinfilin-1-like [Dendronephthya gigantea]
Species Heliopora coerulea · all data for this species · gene families

Gene Structure

More details in JBrowse

Sequence

CDS

Protein

UniProt (Swiss-Prot top hit)

UniProt accession	Description
Q56JV6	Twinfilin-1 OS=Bos taurus OX=9913 GN=TWF1 PE=2 SV=1
Q5R7N2	Twinfilin-1 OS=Pongo abelii OX=9601 GN=TWF1 PE=2 SV=1
Q91YR1	Twinfilin-1 OS=Mus musculus OX=10090 GN=Twf1 PE=1 SV=2

Gene family

Family type	Membership / link
Orthogroup (gene family)	OG0004913 (this species only) · gene tree & orthology

Pfam domain

Pfam accession	Pfam name	Description	Type	Source
PF00241 all species →	Cofilin_ADF	Cofilin/tropomyosin-type actin-binding protein	Domain	Interproscan

InterPro

InterPro term	Type	Description	Source
IPR002108 all species →	Domain	Actin-depolymerising factor homology domain	Interproscan
IPR029006 all species →	Homologous_superfamily	ADF-H/Gelsolin-like domain superfamily	Interproscan
IPR028458 all species →	Family	Twinfilin	Interproscan

PANTHER

PANTHER term	Description	Source
PTHR13759 all species →	TWINFILIN	Interproscan

Gene Ontology

GO term	Category	Description	Source
GO:0003779 all species →	Molecular Function	actin binding	Interproscan
GO:0003785 all species →	Molecular Function	actin monomer binding	Interproscan
GO:0005737 all species →	Cellular Component	cytoplasm	Interproscan
GO:0005884 all species →	Cellular Component	actin filament	Interproscan
GO:0010591 all species →	Biological Process	regulation of lamellipodium assembly	Interproscan
GO:0010976 all species →	Biological Process	positive regulation of neuron projection development	Interproscan
GO:0030016 all species →	Cellular Component	myofibril	Interproscan
GO:0030042 all species →	Biological Process	actin filament depolymerization	Interproscan
GO:0030837 all species →	Biological Process	negative regulation of actin filament polymerization	Interproscan
GO:0042989 all species →	Biological Process	sequestering of actin monomers	Interproscan
GO:0051015 all species →	Molecular Function	actin filament binding	Interproscan
GO:0051016 all species →	Biological Process	barbed-end actin filament capping	Interproscan

Search by domain instead of by gene. Any accession above (InterPro, Pfam, PANTHER, GO, KEGG) can be used as a query on the Functional Domain Search page, which searches all 148 annotated genomes at once.

KEGG pathway

KO	Enzyme	Enzyme ID	Pathway	Map ID	Source
K08870	TWF; twinfilin	-	Cilium and associated proteins	ko03037	deepkoala

Searching by KO or pathway ID across all species is available on the KEGG Pathway page.

Expression pattern (RNA-seq)

Transcript abundance of ENSBQFP00000032001 across 37 RNA-seq samples of Heliopora coerulea. Values are TPM (transcripts per million) from the StringTie quantification; one bar is one sample, grouped and coloured by condition, sorted by expression within each group.

37Samples

36TPM > 0

6Conditions

278.5Max TPM

124.0Mean TPM

By condition

Condition	Samples	TPM > 0	Mean TPM	Max TPM
Whole coral	17	17	172.04	221.05
polyp and skeleton · 31C, 3week	6	6	69.31	90.25
polyp and skeleton · 28C, 3week	6	6	120.01	278.51
polyp and skeleton · 26C, 3week	4	4	79.42	125.05
polyp and skeleton · 28C, 24hr	2	2	65.72	67.43
polyp and skeleton · 31C, 24hr	2	1	38.48	76.97

Per sample · hover a bar for the full sample record

Show the sample table (37 samples)

SRA run	Condition	Tissue	Developmental stage	Treatment	Study	TPM
ERR6178772	Whole coral	Whole coral	not recorded	not recorded	ERP120267	221.05
ERR6178777	Whole coral	Whole coral	not recorded	not recorded	ERP120267	218.86
ERR6178775	Whole coral	Whole coral	not recorded	not recorded	ERP120267	206.34
ERR6178773	Whole coral	Whole coral	not recorded	not recorded	ERP120267	198.03
ERR6178780	Whole coral	Whole coral	not recorded	not recorded	ERP120267	196.95
ERR6178771	Whole coral	Whole coral	not recorded	not recorded	ERP120267	192.94
ERR6178776	Whole coral	Whole coral	not recorded	not recorded	ERP120267	174.74
ERR6178778	Whole coral	Whole coral	not recorded	not recorded	ERP120267	174.48
ERR6178388	Whole coral	Whole coral	not recorded	not recorded	ERP120267	165.89
ERR6178781	Whole coral	Whole coral	not recorded	not recorded	ERP120267	164.95
ERR6178387	Whole coral	Whole coral	not recorded	not recorded	ERP120267	159.44
ERR6178779	Whole coral	Whole coral	not recorded	not recorded	ERP120267	148.99
ERR6178770	Whole coral	Whole coral	not recorded	not recorded	ERP120267	146.36
ERR6178774	Whole coral	Whole coral	not recorded	not recorded	ERP120267	145.48
ERR6178782	Whole coral	Whole coral	not recorded	not recorded	ERP120267	144.76
ERR6178389	Whole coral	Whole coral	not recorded	not recorded	ERP120267	135.05
ERR6178783	Whole coral	Whole coral	not recorded	not recorded	ERP120267	130.32
SRR12578066	polyp and skeleton · 31C, 3week	polyp and skeleton	not recorded	31C, 3week	SRP115860	90.25
SRR12578065	polyp and skeleton · 31C, 3week	polyp and skeleton	not recorded	31C, 3week	SRP115860	76.16
SRR12587799	polyp and skeleton · 31C, 3week	polyp and skeleton	not recorded	31C, 3week	SRP115860	74.77
SRR12587804	polyp and skeleton · 31C, 3week	polyp and skeleton	not recorded	31C, 3week	SRP115860	67.08
SRR12587800	polyp and skeleton · 31C, 3week	polyp and skeleton	not recorded	31C, 3week	SRP115860	62.01
SRR12578068	polyp and skeleton · 31C, 3week	polyp and skeleton	not recorded	31C, 3week	SRP115860	45.58
SRR12578067	polyp and skeleton · 28C, 3week	polyp and skeleton	not recorded	28C, 3week	SRP115860	278.51
SRR5949849	polyp and skeleton · 28C, 3week	polyp and skeleton	not recorded	28C, 3week	SRP115860	157.69
SRR12587808	polyp and skeleton · 28C, 3week	polyp and skeleton	not recorded	28C, 3week	SRP115860	72.71
SRR12587803	polyp and skeleton · 28C, 3week	polyp and skeleton	not recorded	28C, 3week	SRP115860	72.31
SRR12587807	polyp and skeleton · 28C, 3week	polyp and skeleton	not recorded	28C, 3week	SRP115860	69.89
SRR12587802	polyp and skeleton · 28C, 3week	polyp and skeleton	not recorded	28C, 3week	SRP115860	68.96
SRR12587801	polyp and skeleton · 26C, 3week	polyp and skeleton	not recorded	26C, 3week	SRP115860	125.05
SRR12587806	polyp and skeleton · 26C, 3week	polyp and skeleton	not recorded	26C, 3week	SRP115860	65.42
SRR12587798	polyp and skeleton · 26C, 3week	polyp and skeleton	not recorded	26C, 3week	SRP115860	65.31
SRR12587805	polyp and skeleton · 26C, 3week	polyp and skeleton	not recorded	26C, 3week	SRP115860	61.90
SRR5949850	polyp and skeleton · 28C, 24hr	polyp and skeleton	not recorded	28C, 24hr	SRP115860	67.43
SRR12578063	polyp and skeleton · 28C, 24hr	polyp and skeleton	not recorded	28C, 24hr	SRP115860	64.00
SRR12578064	polyp and skeleton · 31C, 24hr	polyp and skeleton	not recorded	31C, 24hr	SRP115860	76.97
SRR5949848	polyp and skeleton · 31C, 24hr	polyp and skeleton	not recorded	31C, 24hr	SRP115860	0.00

Source: CnidoSite RNA-seq expression matrices (HCOER_TPM, StringTie quantification over 37 runs), joined to SRA sample metadata. Samples whose tissue/treatment is not recorded in the source metadata are grouped by the descriptor carried in the expression matrix itself.

Co-expression network

Genes whose expression across the transcriptome samples of Heliopora coerulea tracks this one. Counts are over the whole network; the network view itself draws at most 100 partners per query gene (CNIDO_NET_TOP_K), so a hub gene can show fewer edges than the number below.

Direction	Partners	Most correlated partner	PCC
Positively correlated	33	ENSBQFP00000035653	0.920072065360112
Negatively correlated	86	ENSBQFP00000024368	-0.858516194588846

Open this gene in Network Analysis → The network opens with the gene already entered and both directions selected. There you can add up to 9 more genes, switch between the positive and negative network, and export the edge list.

Single-cell expression

Whether this gene can be visualised in the single-cell atlases of Heliopora coerulea, and in which cell types it is a marker. Expression is stored per cell, so the violin plot and the cell-type means are computed in the viewer — open a dataset to see them.

No single-cell dataset has been published for this species. The atlases that do exist are listed in the Cell Atlas.

Epigenetic marks

Chromatin and DNA-methylation data covering this gene. Peak calls are listed per sample with the genomic region the peak falls in; DNA methylation is listed as sample availability only, because those tables are queried by the DNA Methylation page itself.

No epigenomic data has been deposited for this species. The assays that exist across the site are described in the Epigenomic Data module.

What you can do with this gene

Every tool below opens with this gene already entered, so you land on the analysis rather than on an empty form. Links open in a new tab.

Analysis	What it does	Status
Primer design	Design PCR / qPCR primers on this gene’s sequence with the published primer3 settings. Opens with the primers already computed.	template found	open →
BLAST	Search this gene’s sequence against the CnidoSite BLAST databases. The query is filled in and the species’ database is preselected — press Run to start.	template found	open →
Expression heatmap	Draw this gene’s expression as a heatmap across all RNA-seq samples, and compare it with the genes you add next to it.	ready	open →
Gene family / orthogroup	Look this gene up in the single-copy orthogroups built from the high-quality cnidarian genomes, and see which other species carry an orthologue.		open →
Gene set analysis	Start a gene-set enrichment analysis with this gene as the seed list. Add more genes on that page before running it.		open →