Detailed information of ENSBQFP00000040078.1 in Heliopora coerulea

Genomic Location: JASJOG010000203.1:43332...78324
NR annotation: XP_028415263.1, glutamate synthase [NADH], amyloplastic-like [Dendronephthya gigantea]
Species Heliopora coerulea · all data for this species · gene families


 Gene Structure
More details in JBrowse  Sequence
CDS
Protein
 UniProt (Swiss-Prot top hit)
UniProt accessionDescription
Q03460Glutamate synthase [NADH], amyloplastic OS=Medicago sativa OX=3879 PE=1 SV=1
Q9C102Glutamate synthase [NADH] OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=glt1 PE=1 SV=1
Q0DG35Glutamate synthase 2 [NADH], chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0555600 PE=2 SV=2
 Gene family
Family typeMembership / link
Orthogroup (gene family)OG0002087 (this species only) · gene tree & orthology

 Pfam domain
Pfam accessionPfam nameDescriptionTypeSource
PF04898
all species →
Glu_syn_centralGlutamate synthase central domainDomainInterproscan
PF14691
all species →
Fer4_20Dihydroprymidine dehydrogenase domain II, 4Fe-4S clusterDomainInterproscan
PF00310
all species →
GATase_2Glutamine amidotransferases class-IIDomainInterproscan
PF07992
all species →
Pyr_redox_2Pyridine nucleotide-disulphide oxidoreductaseDomainInterproscan
PF01493
all species →
GXGXGGXGXG motifFamilyInterproscan
PF01645
all species →
Glu_synthaseConserved region in glutamate synthaseDomainInterproscan

 InterPro
InterPro termTypeDescriptionSource
IPR036188
all species →
Homologous_superfamilyFAD/NAD(P)-binding domain superfamilyInterproscan
IPR006982
all species →
DomainGlutamate synthase, central-NInterproscan
IPR036485
all species →
Homologous_superfamilyGlutamate synthase, alpha subunit, C-terminal domain superfamilyInterproscan
IPR029055
all species →
Homologous_superfamilyNucleophile aminohydrolases, N-terminalInterproscan
IPR002932
all species →
DomainGlutamate synthase domainInterproscan
IPR009051
all species →
Homologous_superfamilyAlpha-helical ferredoxinInterproscan
IPR028261
all species →
DomainDihydroprymidine dehydrogenase domain IIInterproscan
IPR013785
all species →
Homologous_superfamilyAldolase-type TIM barrelInterproscan
IPR017932
all species →
DomainGlutamine amidotransferase type 2 domainInterproscan
IPR023753
all species →
DomainFAD/NAD(P)-binding domainInterproscan
IPR002489
all species →
DomainGlutamate synthase, alpha subunit, C-terminalInterproscan
IPR006005
all species →
FamilyGlutamate synthase, NADH/NADPH, small subunit 1Interproscan
IPR051394
all species →
FamilyGlutamate SynthaseInterproscan
IPR012220
all species →
FamilyGlutamate synthase, eukaryoticInterproscan

 PANTHER
PANTHER termDescriptionSource
PTHR43100
all species →
GLUTAMATE SYNTHASE [NADPH] SMALL CHAINInterproscan

 Gene Ontology
GO termCategoryDescriptionSource
GO:0006807
all species →
Biological Processobsolete nitrogen compound metabolic processInterproscan
GO:0015930
all species →
Molecular Functionglutamate synthase activityInterproscan
GO:0016491
all species →
Molecular Functionoxidoreductase activityInterproscan
GO:0006537
all species →
Biological Processglutamate biosynthetic processInterproscan
GO:0016638
all species →
Molecular Functionoxidoreductase activity, acting on the CH-NH2 group of donorsInterproscan
GO:0051536
all species →
Molecular Functioniron-sulfur cluster bindingInterproscan
GO:0016639
all species →
Molecular Functionoxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptorInterproscan
GO:0005506
all species →
Molecular Functioniron ion bindingInterproscan
GO:0010181
all species →
Molecular FunctionFMN bindingInterproscan
GO:0016040
all species →
Molecular Functionglutamate synthase (NADH) activityInterproscan
GO:0050660
all species →
Molecular Functionflavin adenine dinucleotide bindingInterproscan

Search by domain instead of by gene. Any accession above (InterPro, Pfam, PANTHER, GO, KEGG) can be used as a query on the Functional Domain Search page, which searches all 148 annotated genomes at once.
 KEGG pathway
KOEnzymeEnzyme IDPathwayMap IDSource
K00264GLT1; glutamate synthase (NADH)EC:1.4.1.14
Alanine, aspartate and glutamate metabolismko00250deepkoala

Searching by KO or pathway ID across all species is available on the KEGG Pathway page.

Expression pattern (RNA-seq)

Transcript abundance of ENSBQFP00000040078.1 across 37 RNA-seq samples of Heliopora coerulea. This gene has no row in the species' RNA-seq expression matrix, so every value below is shown as zero — the matrix simply does not cover this transcript. Values are TPM (transcripts per million) from the StringTie quantification; one bar is one sample, grouped and coloured by condition, sorted by expression within each group.

37Samples
0TPM > 0
6Conditions
0.0Max TPM
0.0Mean TPM

By condition

ConditionSamplesTPM > 0 Mean TPMMax TPMMean, relative to max
Whole coral 17 0 0.00 0.00
polyp and skeleton · 31C, 3week 6 0 0.00 0.00
polyp and skeleton · 28C, 3week 6 0 0.00 0.00
polyp and skeleton · 26C, 3week 4 0 0.00 0.00
polyp and skeleton · 28C, 24hr 2 0 0.00 0.00
polyp and skeleton · 31C, 24hr 2 0 0.00 0.00

Per sample · hover a bar for the full sample record

Show the sample table (37 samples)
SRA runConditionTissueDevelopmental stage TreatmentStudyTPM
ERR6178387 Whole coral Whole coral not recorded not recorded ERP120267 0.00
ERR6178388 Whole coral Whole coral not recorded not recorded ERP120267 0.00
ERR6178389 Whole coral Whole coral not recorded not recorded ERP120267 0.00
ERR6178770 Whole coral Whole coral not recorded not recorded ERP120267 0.00
ERR6178771 Whole coral Whole coral not recorded not recorded ERP120267 0.00
ERR6178772 Whole coral Whole coral not recorded not recorded ERP120267 0.00
ERR6178773 Whole coral Whole coral not recorded not recorded ERP120267 0.00
ERR6178774 Whole coral Whole coral not recorded not recorded ERP120267 0.00
ERR6178775 Whole coral Whole coral not recorded not recorded ERP120267 0.00
ERR6178776 Whole coral Whole coral not recorded not recorded ERP120267 0.00
ERR6178777 Whole coral Whole coral not recorded not recorded ERP120267 0.00
ERR6178778 Whole coral Whole coral not recorded not recorded ERP120267 0.00
ERR6178779 Whole coral Whole coral not recorded not recorded ERP120267 0.00
ERR6178780 Whole coral Whole coral not recorded not recorded ERP120267 0.00
ERR6178781 Whole coral Whole coral not recorded not recorded ERP120267 0.00
ERR6178782 Whole coral Whole coral not recorded not recorded ERP120267 0.00
ERR6178783 Whole coral Whole coral not recorded not recorded ERP120267 0.00
SRR12578065 polyp and skeleton · 31C, 3week polyp and skeleton not recorded 31C, 3week SRP115860 0.00
SRR12578066 polyp and skeleton · 31C, 3week polyp and skeleton not recorded 31C, 3week SRP115860 0.00
SRR12578068 polyp and skeleton · 31C, 3week polyp and skeleton not recorded 31C, 3week SRP115860 0.00
SRR12587799 polyp and skeleton · 31C, 3week polyp and skeleton not recorded 31C, 3week SRP115860 0.00
SRR12587800 polyp and skeleton · 31C, 3week polyp and skeleton not recorded 31C, 3week SRP115860 0.00
SRR12587804 polyp and skeleton · 31C, 3week polyp and skeleton not recorded 31C, 3week SRP115860 0.00
SRR12578067 polyp and skeleton · 28C, 3week polyp and skeleton not recorded 28C, 3week SRP115860 0.00
SRR12587802 polyp and skeleton · 28C, 3week polyp and skeleton not recorded 28C, 3week SRP115860 0.00
SRR12587803 polyp and skeleton · 28C, 3week polyp and skeleton not recorded 28C, 3week SRP115860 0.00
SRR12587807 polyp and skeleton · 28C, 3week polyp and skeleton not recorded 28C, 3week SRP115860 0.00
SRR12587808 polyp and skeleton · 28C, 3week polyp and skeleton not recorded 28C, 3week SRP115860 0.00
SRR5949849 polyp and skeleton · 28C, 3week polyp and skeleton not recorded 28C, 3week SRP115860 0.00
SRR12587798 polyp and skeleton · 26C, 3week polyp and skeleton not recorded 26C, 3week SRP115860 0.00
SRR12587801 polyp and skeleton · 26C, 3week polyp and skeleton not recorded 26C, 3week SRP115860 0.00
SRR12587805 polyp and skeleton · 26C, 3week polyp and skeleton not recorded 26C, 3week SRP115860 0.00
SRR12587806 polyp and skeleton · 26C, 3week polyp and skeleton not recorded 26C, 3week SRP115860 0.00
SRR12578063 polyp and skeleton · 28C, 24hr polyp and skeleton not recorded 28C, 24hr SRP115860 0.00
SRR5949850 polyp and skeleton · 28C, 24hr polyp and skeleton not recorded 28C, 24hr SRP115860 0.00
SRR12578064 polyp and skeleton · 31C, 24hr polyp and skeleton not recorded 31C, 24hr SRP115860 0.00
SRR5949848 polyp and skeleton · 31C, 24hr polyp and skeleton not recorded 31C, 24hr SRP115860 0.00

Source: CnidoSite RNA-seq expression matrices (HCOER_TPM, StringTie quantification over 37 runs), joined to SRA sample metadata. Samples whose tissue/treatment is not recorded in the source metadata are grouped by the descriptor carried in the expression matrix itself.

Co-expression network

Genes whose expression across the transcriptome samples of Heliopora coerulea tracks this one. Counts are over the whole network; the network view itself draws at most 100 partners per query gene (CNIDO_NET_TOP_K), so a hub gene can show fewer edges than the number below.

DirectionPartnersMost correlated partnerPCC
Positively correlated0not in this network-
Negatively correlated0not in this network-

This gene has no edge at all in the Heliopora coerulea network, in either direction — it is not one of the genes the network was built from (the network covers genes with enough expression variation across the transcriptome samples). The counts above are a property of the network, not a failed lookup.

Open this gene in Network Analysis → The network opens with the gene already entered and both directions selected. There you can add up to 9 more genes, switch between the positive and negative network, and export the edge list.

Single-cell expression

Whether this gene can be visualised in the single-cell atlases of Heliopora coerulea, and in which cell types it is a marker. Expression is stored per cell, so the violin plot and the cell-type means are computed in the viewer — open a dataset to see them.

No single-cell dataset has been published for this species. The atlases that do exist are listed in the Cell Atlas.

Epigenetic marks

Chromatin and DNA-methylation data covering this gene. Peak calls are listed per sample with the genomic region the peak falls in; DNA methylation is listed as sample availability only, because those tables are queried by the DNA Methylation page itself.

No epigenomic data has been deposited for this species. The assays that exist across the site are described in the Epigenomic Data module.

What you can do with this gene

Every tool below opens with this gene already entered, so you land on the analysis rather than on an empty form. Links open in a new tab.

AnalysisWhat it doesStatus
Primer designDesign PCR / qPCR primers on this gene’s sequence with the published primer3 settings. Opens with the primers already computed.template foundopen →
BLASTSearch this gene’s sequence against the CnidoSite BLAST databases. The query is filled in and the species’ database is preselected — press Run to start.template foundopen →
Expression heatmapDraw this gene’s expression as a heatmap across all RNA-seq samples, and compare it with the genes you add next to it.no expression matrix
Gene family / orthogroupLook this gene up in the single-copy orthogroups built from the high-quality cnidarian genomes, and see which other species carry an orthologue.open →
Gene set analysisStart a gene-set enrichment analysis with this gene as the seed list. Add more genes on that page before running it.open →
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