Gene detail

Detailed information of ENSKFPP00000021844.1 in Condylactis gigantea

Genomic Location: chr4:22692305...22701874
NR annotation: XP_031553852.1, nitric oxide synthase, brain-like [Actinia tenebrosa]
Species Condylactis gigantea · all data for this species · gene families

Gene Structure

More details in JBrowse

Sequence

CDS

ENSKFPT00000023647

ATGAGTAACACAAGAACGGTATCCACAAAGCTTGTCAAAGACAAAGTGGGTGGCCTTGGATTCCTTGTCAAAGAAAGGCCAACAAAGCCATGCGTATCAATATCTGGAATTGTCAGAGGCAGTACTGCAGAAGCAAACGGGATGCTTCGTGTTGGTGATGTTTTGTTGGAAGTTAATGGTAAATCCCTTGAAGAGACCTCGTTTGTTAAGGTGTTAGAGATTCTCAATGACATCCCAGTCGGAAGTGTAGTAGCACTAAAGCTTCGAGCTAAGGATGGAACGAAGGCTCAAATCGAGACGACATTTTCTGAGACAGGATCTGCGAATACTTCTGATGTAAAGCCAAAGAAACAGGGAACGGCACTCGAGGAAAATGGAACCCCATCAGTACAGTACCAACAACAAGATCAACAACAGATAACCAATAGTCAAGCTGTTTCAACATCTACTAACGAACTCCCTGAGAACAAGGTAGGAAGTAACACAGCCAGTCCACTGACATCACCCACTATAAAGTGTCCCTTCAATGGAATACTGCGGGAAACCTCCAAGACACCCAAGATCATCAAGCTCAAGAACTGGGAAACTGGCAAGGAATCCATTGATACACTGCATCAGAAAGCTAAACTCCCAATAAACTGTACACCAGCCAGATGTGAAGGGTCATTGATGAGGCCCTATGGTGGAGAAGTAACGCCCTCAAGGGGTTATGGGATACCAAGACCGAAAGACGAAGCGGCTGTCCACGCTGAGGACTTCATAGAACAGTATTACACCTCCATGAAAATGGGTGATAGTCCAGCACACAAGAGGAGACTGGCCGAGGTGCTAAAGGAGATCGAGGAGACTGGTACCTATGACTTGACTGAAAAGGAACTGCTGTTTGGTGTGCGTATGGCATGGAGGAATGCACCAAGATGCATTGGCAGAATACAATGGAACAGGCTGCACCTGTTTGATGCTCGTGATGTCTTCACTCCACGAGGTATGTTTGATGCTATCATTAAACACATCAAATATGGCACCAACAAGGGAAACTTGAGGTCGACCATCACAGTGTTTCCTCAACGAAAAGAACCCAACAAGGATTTCCGAGTGTGGAATGGTCAGTTGATAACATACGCTGGTTATCGTCAGCCCGATGGCTCCATCCTAGGTGACCCGGCTAACGCAGAACTGACTGAAGTCTGTATTTCTCTCGGCTGGGAGCCTAAAGGTGGTCGTTTTGATGTTCTACCGCTTGTCTTACAAGCCGGAGGCGAAGAACCTGAAATGTTCGAAATTCCACCTGAACTGATTTTACAAGTCCAACTCAAGCATCCCAAGTACCCATGGTTCAAGGAGATGGGTCTTCAGTGGTATGCCCTGCCTGCAGTCTCGTGTCTTTGTCTTGATGTTGGGGGCTTGGAGTTTCCTGCCTGCCCTTTTAATGGTATGGGTGTCTACCCCTCACTTGCTTGCAAACCTGTGGCCAAGAAGATGGGTCTAGATACCAAGAGTAACGCTTCACTGTGGAAGGACCTGGCGTGTATCGAGATGAATGTTGCTGTGTTACACAGCTTCCAGGAAGCGAATGTGACCATTATTGACCATCATTCTGCAGCAGAATCTTTCATGAAGCACTTCGAGAATGAGAACAAGCTCCGTGGTGGATGTCCTGGTGACTGGGTGTGGATTGTTCCACCTGTAGCAGGATCGTCTACTCCTGTCTTTCATCAGGAAATGCTCAATTACTCCCTTAAACCAAGCTACGAATACCAGGACGATCCGTGGAAACATCATCGCATCAAGAGGAAAGAATCTGATTCACCCAAAAGGAAGATCACTTTCAAAGAAGTTGCAAAGGCTGTTAAATTTTCTGCTAAACTAATGGGTAACGCCATGGCTAAAAGAACCAAAGCTATCATCCTGTACGCTACTGAGACTGGGAAGTCTGAGAGCTACGCCAAGATGCTCAGTGAGATGTTCTTGCATGCGTTCGATCCAAAGGTGATGTGTATGGATGAATATCCCCATCCAGAACTGGAAAACGAACAGCTCGTCTTGATCGTAACCAGTACGTTTGGGAATGGTGATCCACCAGAGAACGGAGAATCTTTTGCTCGCTACCTTTATGAGCTGCGTCATCCTACAAATGAgaaaacagaaaacaacaagatggctcagatcagtagtctTCTCAtccagaaagaaaaggaaaaggggCTGAAAATGAAGGACGATGAACTACGCTTAGCCAGCCTGAGGTATTCTGTCTTTGGCCTTGGGTCACGTGCTTATCCCAACTTCTGTGCTTTCGCTAAGTCTGTTGACAAAATCATCCAAGAGCTTGGCGGACAGCAGGTTTACAAGATGGGAGAAGGAGATGAATTATGTGGACAGGAAGAGGCCTTCAAGACATGGGCAAAAAATGTCTTTAAGGCTGCATGTGAAACATTCTGTGTTGGAGAAGACGTGAACATCGACGAAGCTGATGCGTCCCTGAACTCTATATCCAGCGGTTGGTCACCTAATAAGTATAGATTTACCAAAAGCAATGAGAAGCTACAAGACATTTATGATGATCACGTCAAAAACATTCTGCGGTTGATATGgccattttgttgttgttctccCAGTCGCGCGACATTGCTCGTACGTCTGAATACGAATGATGACAGGAACTTCAAGTACAAGGCAGGCGACCATCTCTCTCTATTCCCTGCCAACAAGAAATCACTTGTCCAGTCATTACTGGACCGCCTGTGTCAGGCCCCCGATGCTGATCAGCCAATCATAGTCGAGGCTTTCCGTGAAATATCAGGTCCTTTTGGAACGACCAAGAAGTGGGAGAAGCTTGATCGGTTTCCAGTGCCCGTAACTCTGCGAGAAGCATTCACTCGACACATTGATATTACAGGGACACCGACGCCACAGATACTCAACTACCTCGCTACACAGGCTACTGATCCCGAGGAAAAGAAGGCTTTGGAGCAATTAGGAAAGGGTGACACCAGTTACGAGAACTGGAAGTATGACAAACAGGCCAACATTGTTGAAGTATTGGAAGAATTCCCGTCACTCAAGGTCAACGCAGAACTGCTTCTTACACAACTACCTTTACTACAGCCCCGTTTTTATTCCATTTCGTCATCGCAAGACCTCTACCCTGGTGAAGTCCACGTGACCGTAGCTGTAGTTCGGTTTTCAAAGCGAGGTGGACGGGGCCCAGTTCATCACGGCGTGTGTTCAACGTGGCTAGAGAGTCTGGTACCTAATGACATTGTACCATGTTTTGTAAGACATGCGCCCTCGTTCCACCTGCCGGACGATAATTCAATTCCTCTCGTCATGGTCGGACCAGGAACAGGAATCGCTCCCTTCAGAAGCTTCTGGCAACAGCGTCATTTCGACATGGCGAACAAGCCAACCCCACAGGGGTCCTGGGGGGAtatgtttttgtattttggatgtcgaagttcaaaacaggATGATATTTATAGAGATGAGACGGCAGAGGCCAAGAGTGAGAAAGTATTAGCCAACGTTAGAACCGCTCTATCACGAGAGGAAAATCAACCAAAGCGGTATGTACAAGACCTGCTGAAGGAAGACGCATTAGAGATCATCGACACACTGATCAGCCGTCAGGGTCATTTCTATACATGTGGGGATGTTTCCATGGCAGCAGATGTGTGCAGAACAATACAGATGATGCTGGAGTCGTATGCAGCCATGTCACAACAGGAGTCCCAAGACTTTATTGATAAACTGAAGAGCTCTGGAAGATATCACGAGGATATCTTTGGAGTGACACTTCGAACACGTGAAGTAACAGATCGCGTCAGAACTGCAGCAAGGAAGGCTTGGGCTTACATTGCTACTGCAAGATCTGTCAAGGTGAATAACATTGATCAATCACAAACTTCTCATCTTTAA

Transcript

ENSKFPT00000023647

Protein

UniProt (Swiss-Prot top hit)

UniProt accession	Description
Q9Z0J4	Nitric oxide synthase 1 OS=Mus musculus OX=10090 GN=Nos1 PE=1 SV=1
P29476	Nitric oxide synthase 1 OS=Rattus norvegicus OX=10116 GN=Nos1 PE=1 SV=1
P29475	Nitric oxide synthase 1 OS=Homo sapiens OX=9606 GN=NOS1 PE=1 SV=2

Gene family

Family type	Membership / link
Orthogroup (gene family)	OG0001036 (this species only) · gene tree & orthology

Pfam domain

Pfam accession	Pfam name	Description	Type	Source
PF00258 all species →	Flavodoxin_1	Flavodoxin	Domain	Interproscan
PF00175 all species →	NAD_binding_1	Oxidoreductase NAD-binding domain	Domain	Interproscan
PF00595 all species →	PDZ	PDZ domain	Domain	Interproscan
PF02898 all species →	NO_synthase	Nitric oxide synthase, oxygenase domain	Domain	Interproscan
PF00667 all species →	FAD_binding_1	FAD binding domain	Domain	Interproscan

InterPro

InterPro term	Type	Description	Source
IPR001709 all species →	Domain	Flavoprotein pyridine nucleotide cytochrome reductase	Interproscan
IPR008254 all species →	Domain	Flavodoxin/nitric oxide synthase	Interproscan
IPR044944 all species →	Homologous_superfamily	Nitric oxide synthase, domain 3 superfamily	Interproscan
IPR039261 all species →	Homologous_superfamily	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain	Interproscan
IPR017927 all species →	Domain	FAD-binding domain, ferredoxin reductase-type	Interproscan
IPR044943 all species →	Homologous_superfamily	Nitric oxide synthase, domain 1 superfamily	Interproscan
IPR029039 all species →	Homologous_superfamily	Flavoprotein-like superfamily	Interproscan
IPR017938 all species →	Homologous_superfamily	Riboflavin synthase-like beta-barrel	Interproscan
IPR001478 all species →	Domain	PDZ domain	Interproscan
IPR036034 all species →	Homologous_superfamily	PDZ superfamily	Interproscan
IPR012144 all species →	Family	Nitric-oxide synthase, eukaryote	Interproscan
IPR023173 all species →	Homologous_superfamily	NADPH-cytochrome p450 reductase, FAD-binding, alpha-helical domain superfamily	Interproscan
IPR001433 all species →	Domain	Oxidoreductase FAD/NAD(P)-binding	Interproscan
IPR050607 all species →	Family	Nitric Oxide Synthase (NOS)	Interproscan
IPR044940 all species →	Homologous_superfamily	Nitric oxide synthase, domain 2 superfamily	Interproscan
IPR036119 all species →	Homologous_superfamily	Nitric oxide synthase, N-terminal domain superfamily	Interproscan
IPR004030 all species →	Domain	Nitric oxide synthase, N-terminal	Interproscan
IPR003097 all species →	Domain	Sulfite reductase [NADPH] flavoprotein alpha-component-like, FAD-binding	Interproscan
IPR001094 all species →	Domain	Flavodoxin-like	Interproscan

PANTHER

PANTHER term	Description	Source
PTHR43410 all species →	NITRIC OXIDE SYNTHASE OXYGENASE	Interproscan

Gene Ontology

GO term	Category	Description	Source
GO:0016491 all species →	Molecular Function	oxidoreductase activity	Interproscan
GO:0010181 all species →	Molecular Function	FMN binding	Interproscan
GO:0005515 all species →	Molecular Function	protein binding	Interproscan
GO:0004517 all species →	Molecular Function	nitric-oxide synthase activity	Interproscan
GO:0005516 all species →	Molecular Function	calmodulin binding	Interproscan
GO:0006809 all species →	Biological Process	nitric oxide biosynthetic process	Interproscan
GO:0020037 all species →	Molecular Function	heme binding	Interproscan
GO:0050660 all species →	Molecular Function	flavin adenine dinucleotide binding	Interproscan
GO:0050661 all species →	Molecular Function	NADP binding	Interproscan

Search by domain instead of by gene. Any accession above (InterPro, Pfam, PANTHER, GO, KEGG) can be used as a query on the Functional Domain Search page, which searches all 148 annotated genomes at once.

KEGG pathway

KO	Enzyme	Enzyme ID	Pathway	Map ID	Source
K13240	NOS1; nitric-oxide synthase, brain	EC:1.14.13.39	Viral myocarditis	ko05416	deepkoala

Searching by KO or pathway ID across all species is available on the KEGG Pathway page.

Co-expression network

Genes whose expression across the transcriptome samples of Condylactis gigantea tracks this one. Counts are over the whole network; the network view itself draws at most 100 partners per query gene (CNIDO_NET_TOP_K), so a hub gene can show fewer edges than the number below.

No co-expression network has been built for this species, so this gene has no partners to show. Networks are available for the species listed on the Network Analysis page.

Single-cell expression

Whether this gene can be visualised in the single-cell atlases of Condylactis gigantea, and in which cell types it is a marker. Expression is stored per cell, so the violin plot and the cell-type means are computed in the viewer — open a dataset to see them.

No single-cell dataset has been published for this species. The atlases that do exist are listed in the Cell Atlas.

Epigenetic marks

Chromatin and DNA-methylation data covering this gene. Peak calls are listed per sample with the genomic region the peak falls in; DNA methylation is listed as sample availability only, because those tables are queried by the DNA Methylation page itself.

No epigenomic data has been deposited for this species. The assays that exist across the site are described in the Epigenomic Data module.

What you can do with this gene

Every tool below opens with this gene already entered, so you land on the analysis rather than on an empty form. Links open in a new tab.

Analysis	What it does	Status
Primer design	Design PCR / qPCR primers on this gene’s sequence with the published primer3 settings. Opens with the primers already computed.	template found	open →
BLAST	Search this gene’s sequence against the CnidoSite BLAST databases. The query is filled in and the species’ database is preselected — press Run to start.	template found	open →
Expression heatmap	Draw this gene’s expression as a heatmap across all RNA-seq samples, and compare it with the genes you add next to it.	no expression matrix	–
Gene family / orthogroup	Look this gene up in the single-copy orthogroups built from the high-quality cnidarian genomes, and see which other species carry an orthologue.		open →
Gene set analysis	Start a gene-set enrichment analysis with this gene as the seed list. Add more genes on that page before running it.		open →