Detailed information of XP_020621978.1 in Orbicella faveolata

Genomic Location: Sc7jCM8_2954:1235294...1264815
NR annotation: XP_020621977.1, putative glutamate synthase [NADPH] [Orbicella faveolata]
Species Orbicella faveolata · all data for this species · gene families


 Gene Structure
More details in JBrowse  Sequence
CDS
Transcript
Protein
 UniProt (Swiss-Prot top hit)
UniProt accessionDescription
Q9C102Glutamate synthase [NADH] OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=glt1 PE=1 SV=1
Q03460Glutamate synthase [NADH], amyloplastic OS=Medicago sativa OX=3879 PE=1 SV=1
Q9LV03Glutamate synthase 1 [NADH], chloroplastic OS=Arabidopsis thaliana OX=3702 GN=GLT1 PE=1 SV=2
 Gene family
Family typeMembership / link
Orthogroup (gene family)OG0002087 (this species only) · gene tree & orthology

 Pfam domain
Pfam accessionPfam nameDescriptionTypeSource
PF00310
all species →
GATase_2Glutamine amidotransferases class-IIDomainInterproscan
PF07992
all species →
Pyr_redox_2Pyridine nucleotide-disulphide oxidoreductaseDomainInterproscan
PF01493
all species →
GXGXGGXGXG motifFamilyInterproscan
PF14691
all species →
Fer4_20Dihydroprymidine dehydrogenase domain II, 4Fe-4S clusterDomainInterproscan
PF04898
all species →
Glu_syn_centralGlutamate synthase central domainDomainInterproscan
PF01645
all species →
Glu_synthaseConserved region in glutamate synthaseDomainInterproscan

 InterPro
InterPro termTypeDescriptionSource
IPR002932
all species →
DomainGlutamate synthase domainInterproscan
IPR017932
all species →
DomainGlutamine amidotransferase type 2 domainInterproscan
IPR036485
all species →
Homologous_superfamilyGlutamate synthase, alpha subunit, C-terminal domain superfamilyInterproscan
IPR029055
all species →
Homologous_superfamilyNucleophile aminohydrolases, N-terminalInterproscan
IPR013785
all species →
Homologous_superfamilyAldolase-type TIM barrelInterproscan
IPR009051
all species →
Homologous_superfamilyAlpha-helical ferredoxinInterproscan
IPR006005
all species →
FamilyGlutamate synthase, NADH/NADPH, small subunit 1Interproscan
IPR002489
all species →
DomainGlutamate synthase, alpha subunit, C-terminalInterproscan
IPR012220
all species →
FamilyGlutamate synthase, eukaryoticInterproscan
IPR051394
all species →
FamilyGlutamate SynthaseInterproscan
IPR023753
all species →
DomainFAD/NAD(P)-binding domainInterproscan
IPR028261
all species →
DomainDihydroprymidine dehydrogenase domain IIInterproscan
IPR036188
all species →
Homologous_superfamilyFAD/NAD(P)-binding domain superfamilyInterproscan
IPR006982
all species →
DomainGlutamate synthase, central-NInterproscan

 PANTHER
PANTHER termDescriptionSource
PTHR43100
all species →
GLUTAMATE SYNTHASE [NADPH] SMALL CHAINInterproscan

 Gene Ontology
GO termCategoryDescriptionSource
GO:0006537
all species →
Biological Processglutamate biosynthetic processInterproscan
GO:0015930
all species →
Molecular Functionglutamate synthase activityInterproscan
GO:0016638
all species →
Molecular Functionoxidoreductase activity, acting on the CH-NH2 group of donorsInterproscan
GO:0016491
all species →
Molecular Functionoxidoreductase activityInterproscan
GO:0051536
all species →
Molecular Functioniron-sulfur cluster bindingInterproscan
GO:0016639
all species →
Molecular Functionoxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptorInterproscan
GO:0005506
all species →
Molecular Functioniron ion bindingInterproscan
GO:0010181
all species →
Molecular FunctionFMN bindingInterproscan
GO:0016040
all species →
Molecular Functionglutamate synthase (NADH) activityInterproscan
GO:0050660
all species →
Molecular Functionflavin adenine dinucleotide bindingInterproscan
GO:0006807
all species →
Biological Processobsolete nitrogen compound metabolic processInterproscan

Search by domain instead of by gene. Any accession above (InterPro, Pfam, PANTHER, GO, KEGG) can be used as a query on the Functional Domain Search page, which searches all 148 annotated genomes at once.
 KEGG pathway
KOEnzymeEnzyme IDPathwayMap IDSource
K00264GLT1; glutamate synthase (NADH)EC:1.4.1.14
Alanine, aspartate and glutamate metabolismko00250deepkoala

Searching by KO or pathway ID across all species is available on the KEGG Pathway page.

Expression pattern (RNA-seq)

Transcript abundance of XP_020621978.1 across 70 RNA-seq samples of Orbicella faveolata. Values are TPM (transcripts per million) from the StringTie quantification; one bar is one sample, grouped and coloured by condition, sorted by expression within each group.

70Samples
33TPM > 0
4Conditions
44.8Max TPM
7.1Mean TPM

By condition

ConditionSamplesTPM > 0 Mean TPMMax TPMMean, relative to max
holobiont · control.pH_high.temp 21 12 7.07 34.33
holobiont · low.pH_high.temp 19 6 5.46 23.91
holobiont · low.pH Control.temp 16 8 7.81 44.82
holobiont · control.pH Control.temp 14 7 8.54 32.81

Per sample · hover a bar for the full sample record

Show the sample table (70 samples)
SRA runConditionTissueDevelopmental stage TreatmentStudyTPM
SRR22214539 holobiont · control.pH_high.temp holobiont not recorded control.pH_high.temp SRP406607 34.33
SRR22214532 holobiont · control.pH_high.temp holobiont not recorded control.pH_high.temp SRP406607 25.64
SRR22214538 holobiont · control.pH_high.temp holobiont not recorded control.pH_high.temp SRP406607 20.11
SRR22214542 holobiont · control.pH_high.temp holobiont not recorded control.pH_high.temp SRP406607 16.93
SRR22214502 holobiont · control.pH_high.temp holobiont not recorded control.pH_high.temp SRP406607 12.26
SRR22214497 holobiont · control.pH_high.temp holobiont not recorded control.pH_high.temp SRP406607 11.40
SRR22214536 holobiont · control.pH_high.temp holobiont not recorded control.pH_high.temp SRP406607 8.05
SRR22214541 holobiont · control.pH_high.temp holobiont not recorded control.pH_high.temp SRP406607 5.77
SRR22214534 holobiont · control.pH_high.temp holobiont not recorded control.pH_high.temp SRP406607 4.61
SRR22214543 holobiont · control.pH_high.temp holobiont not recorded control.pH_high.temp SRP406607 3.60
SRR22214501 holobiont · control.pH_high.temp holobiont not recorded control.pH_high.temp SRP406607 3.12
SRR22214498 holobiont · control.pH_high.temp holobiont not recorded control.pH_high.temp SRP406607 2.69
SRR22214496 holobiont · control.pH_high.temp holobiont not recorded control.pH_high.temp SRP406607 0.00
SRR22214499 holobiont · control.pH_high.temp holobiont not recorded control.pH_high.temp SRP406607 0.00
SRR22214500 holobiont · control.pH_high.temp holobiont not recorded control.pH_high.temp SRP406607 0.00
SRR22214531 holobiont · control.pH_high.temp holobiont not recorded control.pH_high.temp SRP406607 0.00
SRR22214533 holobiont · control.pH_high.temp holobiont not recorded control.pH_high.temp SRP406607 0.00
SRR22214537 holobiont · control.pH_high.temp holobiont not recorded control.pH_high.temp SRP406607 0.00
SRR22214540 holobiont · control.pH_high.temp holobiont not recorded control.pH_high.temp SRP406607 0.00
SRR22214544 holobiont · control.pH_high.temp holobiont not recorded control.pH_high.temp SRP406607 0.00
SRR22214545 holobiont · control.pH_high.temp holobiont not recorded control.pH_high.temp SRP406607 0.00
SRR22214473 holobiont · low.pH_high.temp holobiont not recorded low.pH_high.temp SRP406607 23.91
SRR22214485 holobiont · low.pH_high.temp holobiont not recorded low.pH_high.temp SRP406607 20.75
SRR22214490 holobiont · low.pH_high.temp holobiont not recorded low.pH_high.temp SRP406607 17.66
SRR22214479 holobiont · low.pH_high.temp holobiont not recorded low.pH_high.temp SRP406607 15.84
SRR22214488 holobiont · low.pH_high.temp holobiont not recorded low.pH_high.temp SRP406607 13.39
SRR22214476 holobiont · low.pH_high.temp holobiont not recorded low.pH_high.temp SRP406607 12.10
SRR22214472 holobiont · low.pH_high.temp holobiont not recorded low.pH_high.temp SRP406607 0.00
SRR22214474 holobiont · low.pH_high.temp holobiont not recorded low.pH_high.temp SRP406607 0.00
SRR22214475 holobiont · low.pH_high.temp holobiont not recorded low.pH_high.temp SRP406607 0.00
SRR22214477 holobiont · low.pH_high.temp holobiont not recorded low.pH_high.temp SRP406607 0.00
SRR22214478 holobiont · low.pH_high.temp holobiont not recorded low.pH_high.temp SRP406607 0.00
SRR22214480 holobiont · low.pH_high.temp holobiont not recorded low.pH_high.temp SRP406607 0.00
SRR22214482 holobiont · low.pH_high.temp holobiont not recorded low.pH_high.temp SRP406607 0.00
SRR22214483 holobiont · low.pH_high.temp holobiont not recorded low.pH_high.temp SRP406607 0.00
SRR22214484 holobiont · low.pH_high.temp holobiont not recorded low.pH_high.temp SRP406607 0.00
SRR22214486 holobiont · low.pH_high.temp holobiont not recorded low.pH_high.temp SRP406607 0.00
SRR22214487 holobiont · low.pH_high.temp holobiont not recorded low.pH_high.temp SRP406607 0.00
SRR22214489 holobiont · low.pH_high.temp holobiont not recorded low.pH_high.temp SRP406607 0.00
SRR22214491 holobiont · low.pH_high.temp holobiont not recorded low.pH_high.temp SRP406607 0.00
SRR22214530 holobiont · low.pH Control.temp holobiont not recorded low.pH Control.temp SRP406607 44.82
SRR22214522 holobiont · low.pH Control.temp holobiont not recorded low.pH Control.temp SRP406607 16.34
SRR22214494 holobiont · low.pH Control.temp holobiont not recorded low.pH Control.temp SRP406607 15.64
SRR22214521 holobiont · low.pH Control.temp holobiont not recorded low.pH Control.temp SRP406607 15.46
SRR22214526 holobiont · low.pH Control.temp holobiont not recorded low.pH Control.temp SRP406607 10.15
SRR22214525 holobiont · low.pH Control.temp holobiont not recorded low.pH Control.temp SRP406607 9.57
SRR22214520 holobiont · low.pH Control.temp holobiont not recorded low.pH Control.temp SRP406607 9.13
SRR22214529 holobiont · low.pH Control.temp holobiont not recorded low.pH Control.temp SRP406607 3.77
SRR22214493 holobiont · low.pH Control.temp holobiont not recorded low.pH Control.temp SRP406607 0.00
SRR22214495 holobiont · low.pH Control.temp holobiont not recorded low.pH Control.temp SRP406607 0.00
SRR22214517 holobiont · low.pH Control.temp holobiont not recorded low.pH Control.temp SRP406607 0.00
SRR22214518 holobiont · low.pH Control.temp holobiont not recorded low.pH Control.temp SRP406607 0.00
SRR22214519 holobiont · low.pH Control.temp holobiont not recorded low.pH Control.temp SRP406607 0.00
SRR22214523 holobiont · low.pH Control.temp holobiont not recorded low.pH Control.temp SRP406607 0.00
SRR22214527 holobiont · low.pH Control.temp holobiont not recorded low.pH Control.temp SRP406607 0.00
SRR22214528 holobiont · low.pH Control.temp holobiont not recorded low.pH Control.temp SRP406607 0.00
SRR22214509 holobiont · control.pH Control.temp holobiont not recorded control.pH Control.temp SRP406607 32.81
SRR22214504 holobiont · control.pH Control.temp holobiont not recorded control.pH Control.temp SRP406607 30.02
SRR22214510 holobiont · control.pH Control.temp holobiont not recorded control.pH Control.temp SRP406607 21.07
SRR22214507 holobiont · control.pH Control.temp holobiont not recorded control.pH Control.temp SRP406607 18.30
SRR22214513 holobiont · control.pH Control.temp holobiont not recorded control.pH Control.temp SRP406607 10.53
SRR22214515 holobiont · control.pH Control.temp holobiont not recorded control.pH Control.temp SRP406607 4.62
SRR22214514 holobiont · control.pH Control.temp holobiont not recorded control.pH Control.temp SRP406607 2.18
SRR22214401 holobiont · control.pH Control.temp holobiont not recorded control.pH Control.temp SRP406607 0.00
SRR22214503 holobiont · control.pH Control.temp holobiont not recorded control.pH Control.temp SRP406607 0.00
SRR22214505 holobiont · control.pH Control.temp holobiont not recorded control.pH Control.temp SRP406607 0.00
SRR22214508 holobiont · control.pH Control.temp holobiont not recorded control.pH Control.temp SRP406607 0.00
SRR22214511 holobiont · control.pH Control.temp holobiont not recorded control.pH Control.temp SRP406607 0.00
SRR22214512 holobiont · control.pH Control.temp holobiont not recorded control.pH Control.temp SRP406607 0.00
SRR22214516 holobiont · control.pH Control.temp holobiont not recorded control.pH Control.temp SRP406607 0.00

Source: CnidoSite RNA-seq expression matrices (OFAVE_TPM, StringTie quantification over 70 runs), joined to SRA sample metadata. Samples whose tissue/treatment is not recorded in the source metadata are grouped by the descriptor carried in the expression matrix itself.

Co-expression network

Genes whose expression across the transcriptome samples of Orbicella faveolata tracks this one. Counts are over the whole network; the network view itself draws at most 100 partners per query gene (CNIDO_NET_TOP_K), so a hub gene can show fewer edges than the number below.

DirectionPartnersMost correlated partnerPCC
Positively correlated6XP_020605671.10.751340630834725
Negatively correlated3XP_020610713.1-0.287280764416415

Open this gene in Network Analysis → The network opens with the gene already entered and both directions selected. There you can add up to 9 more genes, switch between the positive and negative network, and export the edge list.

Single-cell expression

Whether this gene can be visualised in the single-cell atlases of Orbicella faveolata, and in which cell types it is a marker. Expression is stored per cell, so the violin plot and the cell-type means are computed in the viewer — open a dataset to see them.

No single-cell dataset has been published for this species. The atlases that do exist are listed in the Cell Atlas.

Epigenetic marks

Chromatin and DNA-methylation data covering this gene. Peak calls are listed per sample with the genomic region the peak falls in; DNA methylation is listed as sample availability only, because those tables are queried by the DNA Methylation page itself.

No epigenomic data has been deposited for this species. The assays that exist across the site are described in the Epigenomic Data module.

What you can do with this gene

Every tool below opens with this gene already entered, so you land on the analysis rather than on an empty form. Links open in a new tab.

AnalysisWhat it doesStatus
Primer designDesign PCR / qPCR primers on this gene’s sequence with the published primer3 settings. Opens with the primers already computed.template foundopen →
BLASTSearch this gene’s sequence against the CnidoSite BLAST databases. The query is filled in and the species’ database is preselected — press Run to start.template foundopen →
Expression heatmapDraw this gene’s expression as a heatmap across all RNA-seq samples, and compare it with the genes you add next to it.readyopen →
Gene family / orthogroupLook this gene up in the single-copy orthogroups built from the high-quality cnidarian genomes, and see which other species carry an orthologue.open →
Gene set analysisStart a gene-set enrichment analysis with this gene as the seed list. Add more genes on that page before running it.open →
TOP