Gene detail

Detailed information of XP_066934387.1 in Clytia hemisphaerica

Genomic Location: NW_027104528.1:1423035...1437529
NR annotation: XP_031552876.1, peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase-like [Actinia tenebrosa]
Species Clytia hemisphaerica · all data for this species · gene families

Gene Structure

More details in JBrowse

Sequence

CDS

XP_066934387.1

ATGGCGACCGCTACAGCTGTCGTAGAAATTCTTTCTGATATTAATTTTTCAAAAGAGTTATCACAAACACCATCCGAACAGCTTGTGGTTGTAGACTTTTATGCAGAATGGTGTGGACCATGTAAATATGTGGCACCTATTCTTGCAGAGCTGAGTTTGAAGTACAAAAATGTGAAATTCCTGAAAGTTGATGTTGACAAATGTCAGGCCACATCAGTAGCAAACGGCATCGAAGCAATGCCAACGTTTCTTTTCTTCAAAAACAACACAAAGATTGACAGCCTTCGTGGAGCAGATACCAAGAGACTAGAGCAAATAATTGTGACACACATGGGAAAATATATTACGGAACCTGATCCTGTGAGAGTTGCAACGGGAAGGAGCTATCAATGTTTTGAAGATCTTTCCAAAAACGAACCGAGTGTTTTTAAAGAAACAGGTGGTATCATACTTAGATATGCTAACAATATTCTGAGGGATCCAGAAAACATGAAGTACCGACAAATCAGATTAGAAAATAAAATTATCATGGAAAAGGTTTTGCCAACTAATGGTGCATTTGATACATTTTTTACTATGGGATTTCAAGAGATGGATGATAAACTATTCCTTCCTCATGGATCCAATTTGGAAGTTGTCCACCAGATTCGTGATGCTTTGACAGATGCTTTCAAAAACCTTGATAAAGATGAAAACAACAAAACGACACCTCAAACTTCATCATCCGCAGTATCAGAGACTGCATCAACTGTTTGCTCAACATCAAACACTGCATCATGTCGATCAACCTTGTTGGAAAGTGTTCCAGTTCCTGGTAAAAGGAAACTTAGATTTAATCCAGCAGGTCTGAATAATCACAAAACCCAATTTTTCCATTCCTTATCATCGCACACAAATCAAGTATTGTTATATGAAGAAGAAGCTTTGCAGATCATTGCGCGATCTCACATCCCTTTAGATGAGCTGAAAACGAAAGCTGAAAAGGAGTATTCCAAAACAAAGAATTTGAATTCAACTTCTTCATATGAAGATTGTTTATTGTTGGAGATGCTTACTTGGTTTAAAAATGATTTTTTCTCTTGGATGAATTCGCCCAAATGTACAAGATGTGAATCAGAAACCAAATCGATTGGTATGCTTCAGCCATCTCCAGATGATTTGAAATGGGGCGCTGGTCGTGTTGAAGGATATCAGTGTAAAGTGTGTGGAACTCAAGTACGCTTTCGACGTTATAATCATCCGCAAAAGCTTTTAGAAACGAGGACGGGTAGATGTGGAGAATGGGCTAATTGTTTCACCTTATGCTGTAGAGCGGTTGGTTTAGAGGCTAGATACGTGCTAGACTGGACAGATCACGTGTGGACAGAAGTCTATTCGAAAAGCAAAAAACGTTGGTTGCACTGTGATTCTTGTGAAAACTCCTGTGATAAACCGTTAACATATGAAGCAGGGTGGGGCAAAGAGTTGTCTTATGTCGTGGCTTTCTCGAAGGATGAGGTGATCGATGTAACATGGAGATATACCAATAAATTTGCTGATGTCATGAAAAGACGAAATAAAGTACCTGAGAAATGGATCATAGATATGTGTCAATCTATCACCAAAGAGCTACGATACGGCGCAACGGAAGCTGAGAGGCAGGAATTCACAACAAGACACATCAAAGAAATAGTCGAATTCATTTCACCCAAAGGCGTGAAGGCGGAGGAGCAACAGGGGCGTCAATCAGGAAGTACGAATTGGCGTTCTATGCGTGGAGAAATGGGCGATCCCTCGAAACTCGGCTACTCGTTCAAACTTTCAGACGAACACAAGAGAAATAAAAGATTCCGAATGCGATACAACCCAGTCAAGGATGAATATTACACTAACACATCAGAGATGATCAGATCAAATGAACCGGCAACTGTCGAAGGATGGTCTAATGGTTTACTGGCAATCGAAAACATCCACCGAAAAGTAGAACACGACTGGAAAATGGTTTATCTAGCTCGTACTGAAGGAACAAATTACGCCAATCTTCAATGGCGGCTTGATTTGAAAAACTCTGGATTTAAAGTAAAAAGATTGACAATTGTTGCCGACATGAAATGTTTCGAAACTGGTCAAATAGTCTTCAATGTTTCCACTGATGGTATCTTCAAGTTGTGTAATCAACAAAGCAAGCAAGAAGGTTTCACATTTTCAACGAGTGAATTTAAAGGTGAGGATGAACACGTGACCATAACAGCTCATCTGAGTGGTGGAAAAGGAGACGTTGCCTGGCAACACACACAATTGTTTAGAACATCACTGGATGTGTCTGATTACATTGGTCTAGAAATTGACGTCGAATTGGATGAGAAATAG

Protein

UniProt (Swiss-Prot top hit)

UniProt accession	Description
Q96IV0	Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Homo sapiens OX=9606 GN=NGLY1 PE=1 SV=1
Q4R6F3	Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Macaca fascicularis OX=9541 GN=NGLY1 PE=2 SV=1
Q5ZJM3	Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Gallus gallus OX=9031 GN=NGLY1 PE=2 SV=1

Gene family

Family type	Membership / link
Orthogroup (gene family)	OG0002168 (this species only) · gene tree & orthology

Pfam domain

Pfam accession	Pfam name	Description	Type	Source
PF01841 all species →	Transglut_core	Transglutaminase-like superfamily	Family	Interproscan
PF00085 all species →	Thioredoxin	Thioredoxin	Domain	Interproscan
PF04721 all species →	PAW	PNGase C-terminal domain, mannose-binding module PAW	Domain	Interproscan
PF09409 all species →	PUB	PUB domain	Domain	Interproscan

InterPro

InterPro term	Type	Description	Source
IPR038765 all species →	Homologous_superfamily	Papain-like cysteine peptidase superfamily	Interproscan
IPR006588 all species →	Domain	Peptide N glycanase, PAW domain	Interproscan
IPR002931 all species →	Domain	Transglutaminase-like	Interproscan
IPR038680 all species →	Homologous_superfamily	PAW domain superfamily	Interproscan
IPR013766 all species →	Domain	Thioredoxin domain	Interproscan
IPR036339 all species →	Homologous_superfamily	PUB-like domain superfamily	Interproscan
IPR050883 all species →	Family	Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase	Interproscan
IPR008979 all species →	Homologous_superfamily	Galactose-binding-like domain superfamily	Interproscan
IPR036249 all species →	Homologous_superfamily	Thioredoxin-like superfamily	Interproscan
IPR017937 all species →	Conserved_site	Thioredoxin, conserved site	Interproscan
IPR018997 all species →	Domain	PUB domain	Interproscan

PANTHER

PANTHER term	Description	Source
PTHR12143 all species →	PEPTIDE N-GLYCANASE PNGASE -RELATED	Interproscan

Gene Ontology

GO term	Category	Description	Source
GO:0005737 all species →	Cellular Component	cytoplasm	Interproscan
GO:0006516 all species →	Biological Process	glycoprotein catabolic process	Interproscan
GO:0000224 all species →	Molecular Function	peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity	Interproscan
GO:0005634 all species →	Cellular Component	nucleus	Interproscan
GO:0005829 all species →	Cellular Component	cytosol	Interproscan
GO:0006515 all species →	Biological Process	protein quality control for misfolded or incompletely synthesized proteins	Interproscan
GO:0006517 all species →	Biological Process	protein deglycosylation	Interproscan

Search by domain instead of by gene. Any accession above (InterPro, Pfam, PANTHER, GO, KEGG) can be used as a query on the Functional Domain Search page, which searches all 148 annotated genomes at once.

KEGG pathway

KO	Enzyme	Enzyme ID	Pathway	Map ID	Source
K01456	E3.5.1.52, NGLY1, PNG1; peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase	EC:3.5.1.52	Protein processing in endoplasmic reticulum	ko04141	deepkoala

Searching by KO or pathway ID across all species is available on the KEGG Pathway page.

Expression pattern (RNA-seq)

Transcript abundance of XP_066934387.1 across 40 RNA-seq samples of Clytia hemisphaerica. Values are TPM (transcripts per million) from the StringTie quantification; one bar is one sample, grouped and coloured by condition, sorted by expression within each group.

40Samples

40TPM > 0

16Conditions

34.0Max TPM

24.0Mean TPM

By condition

Condition	Samples	TPM > 0	Mean TPM	Max TPM
Mature medusa	5	5	24.27	27.22
medusa · Experiment Condition A1	4	4	23.46	26.77
medusa · Experiment Condition A2	4	4	22.26	23.36
medusa · Experiment Condition A3	4	4	14.62	16.00
Early gastrula	2	2	30.98	32.79
Planula 24hpf	2	2	30.72	31.26
Planula 48hpf	2	2	23.02	23.95
Planula 72hpf	2	2	24.22	24.60
Primary polyp	2	2	31.25	34.00
Gastrozooid	2	2	26.16	27.79
Gonozooid	2	2	27.26	28.56
Stolon	2	2	29.12	29.34
Baby medusa	2	2	22.13	23.00
medusa · Experiment Condition B1	2	2	18.20	19.88
medusa · Experiment Condition B2	2	2	23.33	23.75
Mixed	1	1	24.12	24.12

Per sample · hover a bar for the full sample record

Show the sample table (40 samples)

SRA run	Condition	Tissue	Developmental stage	Treatment	Study	TPM
ERR2862245	Mature medusa	not recorded	Mature medusa	not recorded	ERP110164	27.22
ERR2816251	Mature medusa	not recorded	Mature medusa	not recorded	ERP110164	24.90
ERR2816249	Mature medusa	not recorded	Mature medusa	not recorded	ERP110164	24.78
ERR2816250	Mature medusa	not recorded	Mature medusa	not recorded	ERP110164	24.70
ERR2816248	Mature medusa	not recorded	Mature medusa	not recorded	ERP110164	19.75
ERR3299478	medusa · Experiment Condition A1	not recorded	medusa	Experiment Condition A1	ERP115020	26.77
ERR3299477	medusa · Experiment Condition A1	not recorded	medusa	Experiment Condition A1	ERP115020	24.54
ERR3299476	medusa · Experiment Condition A1	not recorded	medusa	Experiment Condition A1	ERP115020	21.37
ERR3299475	medusa · Experiment Condition A1	not recorded	medusa	Experiment Condition A1	ERP115020	21.16
ERR3299481	medusa · Experiment Condition A2	not recorded	medusa	Experiment Condition A2	ERP115020	23.36
ERR3299479	medusa · Experiment Condition A2	not recorded	medusa	Experiment Condition A2	ERP115020	23.23
ERR3299480	medusa · Experiment Condition A2	not recorded	medusa	Experiment Condition A2	ERP115020	21.69
ERR3299482	medusa · Experiment Condition A2	not recorded	medusa	Experiment Condition A2	ERP115020	20.76
ERR3299483	medusa · Experiment Condition A3	not recorded	medusa	Experiment Condition A3	ERP115020	16.00
ERR3299484	medusa · Experiment Condition A3	not recorded	medusa	Experiment Condition A3	ERP115020	15.75
ERR3299486	medusa · Experiment Condition A3	not recorded	medusa	Experiment Condition A3	ERP115020	14.03
ERR3299485	medusa · Experiment Condition A3	not recorded	medusa	Experiment Condition A3	ERP115020	12.68
ERR2816230	Early gastrula	not recorded	Early gastrula	not recorded	ERP110164	32.79
ERR2816231	Early gastrula	not recorded	Early gastrula	not recorded	ERP110164	29.18
ERR2816233	Planula 24hpf	not recorded	Planula 24hpf	not recorded	ERP110164	31.26
ERR2816232	Planula 24hpf	not recorded	Planula 24hpf	not recorded	ERP110164	30.18
ERR2816234	Planula 48hpf	not recorded	Planula 48hpf	not recorded	ERP110164	23.95
ERR2816235	Planula 48hpf	not recorded	Planula 48hpf	not recorded	ERP110164	22.09
ERR2816237	Planula 72hpf	not recorded	Planula 72hpf	not recorded	ERP110164	24.60
ERR2816236	Planula 72hpf	not recorded	Planula 72hpf	not recorded	ERP110164	23.84
ERR2816239	Primary polyp	not recorded	Primary polyp	not recorded	ERP110164	34.00
ERR2816238	Primary polyp	not recorded	Primary polyp	not recorded	ERP110164	28.49
ERR2816240	Gastrozooid	not recorded	Gastrozooid	not recorded	ERP110164	27.79
ERR2816241	Gastrozooid	not recorded	Gastrozooid	not recorded	ERP110164	24.53
ERR2816243	Gonozooid	not recorded	Gonozooid	not recorded	ERP110164	28.56
ERR2816242	Gonozooid	not recorded	Gonozooid	not recorded	ERP110164	25.95
ERR2816244	Stolon	not recorded	Stolon	not recorded	ERP110164	29.34
ERR2816245	Stolon	not recorded	Stolon	not recorded	ERP110164	28.89
ERR2816247	Baby medusa	not recorded	Baby medusa	not recorded	ERP110164	23.00
ERR2816246	Baby medusa	not recorded	Baby medusa	not recorded	ERP110164	21.26
ERR3299472	medusa · Experiment Condition B1	not recorded	medusa	Experiment Condition B1	ERP115020	19.88
ERR3299471	medusa · Experiment Condition B1	not recorded	medusa	Experiment Condition B1	ERP115020	16.53
ERR3299474	medusa · Experiment Condition B2	not recorded	medusa	Experiment Condition B2	ERP115020	23.75
ERR3299473	medusa · Experiment Condition B2	not recorded	medusa	Experiment Condition B2	ERP115020	22.92
ERR2862244	Mixed	not recorded	Mixed	not recorded	ERP110164	24.12

Source: CnidoSite RNA-seq expression matrices (CHEMI_TPM, StringTie quantification over 40 runs), joined to SRA sample metadata. Samples whose tissue/treatment is not recorded in the source metadata are grouped by the descriptor carried in the expression matrix itself.

Co-expression network

Genes whose expression across the transcriptome samples of Clytia hemisphaerica tracks this one. Counts are over the whole network; the network view itself draws at most 100 partners per query gene (CNIDO_NET_TOP_K), so a hub gene can show fewer edges than the number below.

Direction	Partners	Most correlated partner	PCC
Positively correlated	12	XP_066919386.1	0.860060233399999
Negatively correlated	53	XP_066923936.1	-0.81605247105241

Open this gene in Network Analysis → The network opens with the gene already entered and both directions selected. There you can add up to 9 more genes, switch between the positive and negative network, and export the edge list.

Single-cell expression

Whether this gene can be visualised in the single-cell atlases of Clytia hemisphaerica, and in which cell types it is a marker. Expression is stored per cell, so the violin plot and the cell-type means are computed in the viewer — open a dataset to see them.

No single-cell dataset has been published for this species. The atlases that do exist are listed in the Cell Atlas.

Epigenetic marks

Chromatin and DNA-methylation data covering this gene. Peak calls are listed per sample with the genomic region the peak falls in; DNA methylation is listed as sample availability only, because those tables are queried by the DNA Methylation page itself.

No epigenomic data has been deposited for this species. The assays that exist across the site are described in the Epigenomic Data module.

What you can do with this gene

Every tool below opens with this gene already entered, so you land on the analysis rather than on an empty form. Links open in a new tab.

Analysis	What it does	Status
Primer design	Design PCR / qPCR primers on this gene’s sequence with the published primer3 settings. Opens with the primers already computed.	template found	open →
BLAST	Search this gene’s sequence against the CnidoSite BLAST databases. The query is filled in and the species’ database is preselected — press Run to start.	template found	open →
Expression heatmap	Draw this gene’s expression as a heatmap across all RNA-seq samples, and compare it with the genes you add next to it.	ready	open →
Gene family / orthogroup	Look this gene up in the single-copy orthogroups built from the high-quality cnidarian genomes, and see which other species carry an orthologue.		open →
Gene set analysis	Start a gene-set enrichment analysis with this gene as the seed list. Add more genes on that page before running it.		open →