Gene detail

Detailed information of XP_068731291.1 in Montipora capricornis

Genomic Location: chr12:18486798...18504585
NR annotation: XP_029189548.2, crossover junction endonuclease MUS81-like isoform X1 [Acropora millepora]
Species Montipora capricornis · all data for this species · gene families

Gene Structure

More details in JBrowse

Sequence

CDS

Transcript

rna-XM_068875190.1

CAGGCGCCCATCGTCGAAGGGCAAAGTTGAGCAAACATGGCGGGTAAATTGCAATCTGGTTCGTCTGGACAAATATATTAAGTTCCATGAAAATGGCCTCAAAACGCCTAAAGAAGCAACGTGAATGCCCCAACCCTCTCTTTTTGAAGTGGCTGGAAGATTGGAGGGATGAAGCAAAACAAAGGAACTCCAAACTGCAGTACACTTACGGAAAGGCAGCTGCAGCTTTGAGGAAGTATCCTTTACCTTTGAGCTGTGGAAAGGATGCAATGATACTTGATAACATTGGAGACATGATTGCTCGGCGGCTGGATAAAGCATTTGAAGAGTATAATGCACACAATCAAGAAGTTTTAGAAAGTGCCAGTATTCAGGAAGATTACAACAACAAAGGAGAAATGGACCCCACGGGTTTATCACTGAATGTTCCTTCTCCACCTTTACTGGGTGTAGACATTGATTTTGGAAGGAAGACAACAACTAAGAAAAAGCCAGTGAGGAGGAAAAGTACTCGTGAGTACATTCCAGCTTACAGATCAGGCCCCTTTGCCTTGTTGCTGACCTTGTACAGGCAAAGTCAAAAGCCCAACTATCGTGGATACATGACACGTGCAGAGCTTCAAAAGGAAGCCCAGCCACTTTGTGATGCTTCATTCACCATGCCTGATCCAGGAAGTCATTATACAGCTTGGTCTTCAATGTCTACTTTGACAAAGAAAGGACTTATTCTGAAGACCAACAATCCTGCAAGGTATACATTGACAGATGCTGGCTGTGTGTTAGTGGCAAAGCTTGAGAATGTTACACGATCTGACAGTTTGACATCAACATCAAATACTTCAAACTCAAGACTTAGTGGTCCGTCCCCACCAGGCTGCAGTGAAAAGAACTTCACTTTCAGTTCAGTTAAGCCATCCACATGGGAAAATAACAAGCCAGAATGGGATATTTGTGTGGACATTGCCAAACTAAGTGACCCCCATAAACAAGAGATACATGTAGAAAGGTCGGCCAATTTTACCAAGGttaaagaaagaggaaaaaacaATGTTGAACCCAAAAGAATTATTGAAGAAGACAGTGTCTGTGCCTCCCCAGAGTTCGTCCTAAGGCCAGGTACTTTTGATATTCTTTTATGTGTTGATGTCAATGAGACATCAACAGGACAAGCCAAGTCAAGGAAAGAAGGCCTGGCCCCTGAACTGAAGAAAAACGGAGTGCAATTTGATGTGCGTAAATTGCAACTTGGTGACTTCCTGTGGGTGGCGCGAGAACGAACTTTTCCCACACCAGGTTGTTTATCCGTGCCAGTGGCTCGCGAATTAGTGTTGGATCACATCGTTGAAAGAAAGAGAATGGATGATCTTTGTGGAAGTATTATTGATGGACGATTTCGAGAGCAGAAGTTTCGCTTACTCCATTGTGGGCTGAAACACCCTATCTACCTGGTGGAAGATTTTGGTTCCATTCAACACATGAGCCTTCCAGAGAGCACATTACAACAAGCTATTGTCAACACTCAGGTCGTTGACGGTTTTTTTGTGAAGCGGACACACGATTTGAAGGAGTCTGTTGCTTACTTAACAGTGATGACTCGATACCTTCAAAGAATTTACTCAAATAAGACTCTGGTAAGTTGTTCTCAAAGGAGACTTGAGGAAGCCAATTCAAGCACATCAAGACAAACAGATAAGAAGTACCTCATGAGTTTTCCAGACTTCAACGACTCAACTGTTAAGAATAAGgtTATGACAGTGAGAGAGGTATTTGCCAAACAGCTCATGCAGATAAGTGGAATGTCACCAGACAAAGCTGTTGCTATTCTTGATAGATATCCTACTCCCAAAAGcCTTTTTGATGCATACAGTTTACTTAGCAGTGTGAAAGACAAAGAATTGCTTCTCTCCGCTCTGAAGTGCGGCAAAAACCAAAGAAATCTTGGCCCGACCCTGAGCAGACTGATATATATCCTGTACAGCTCTCCTACTCTAAGTTGAGTCAGATGTGGTCTCTTAAACTGCATGGAAAGCAGCAACTAACCCCTTGTAAAGTAACGCAACAGCAATTTTAAAGGGAAAAGAGACACTGCTGCAGAAGTCGATGACAAAATGCTTGGACTCTAACAAGGAAGTACTGTAGACTTGTTACCATTCACCAACGATGACCAGCTTTTTATTAGTCCCAGAAGGATTGTGCATTGTTAATGTTATGCTTTTTTTGGAGGGTGATgcagcagctctcgtaacgagTAATGCCTTTGGCTTGCTACCccagccaaaaacaaaagactaaacaattgaaacaatgacttagactcaaaaacaatagaagctgcttacaataccaaacaaaagcaggttacgagagctgctataCTACCGGTTTTTTTAGTGATTTCTTTCTCTTTTACTGTAAcataaaatgaacaaatttcTTCAGTTTTGTTATTGATTTCTATTCCATTTTGCTCATGGCTGAAATACAATACTCTGTGCTGTAGGATGA

Protein

UniProt (Swiss-Prot top hit)

UniProt accession	Description
Q640B4	Structure-specific endonuclease subunit MUS81 OS=Xenopus tropicalis OX=8364 GN=mus81 PE=2 SV=1
Q7SXA9	Structure-specific endonuclease subunit MUS81 OS=Danio rerio OX=7955 GN=mus81 PE=1 SV=1
Q4KM32	Structure-specific endonuclease subunit MUS81 OS=Rattus norvegicus OX=10116 GN=Mus81 PE=2 SV=1

Gene family

Family type	Membership / link
Orthogroup (gene family)	OG0003511 (this species only) · gene tree & orthology

Pfam domain

Pfam accession	Pfam name	Description	Type	Source
PF21292 all species →	EME1-MUS81_C	EME1/MUS81, C-terminal	Domain	Interproscan
PF21136 all species →	MUS81-like_WH	Crossover junction endonuclease MUS81-like, winged helix domain	Domain	Interproscan
PF02732 all species →	ERCC4	ERCC4 domain	Domain	Interproscan

InterPro

InterPro term	Type	Description	Source
IPR033309 all species →	Family	Crossover junction endonuclease Mus81	Interproscan
IPR047416 all species →	Domain	MUS81, XPF-like nuclease domain	Interproscan
IPR027421 all species →	Homologous_superfamily	DNA polymerase lambda lyase domain superfamily	Interproscan
IPR011335 all species →	Homologous_superfamily	Restriction endonuclease type II-like	Interproscan
IPR047417 all species →	Domain	MUS81, winged helix domain	Interproscan
IPR006166 all species →	Domain	ERCC4 domain	Interproscan
IPR036388 all species →	Homologous_superfamily	Winged helix-like DNA-binding domain superfamily	Interproscan
IPR042530 all species →	Homologous_superfamily	EME1/EME2, C-terminal domain	Interproscan

PANTHER

PANTHER term	Description	Source
PTHR13451 all species →	CLASS II CROSSOVER JUNCTION ENDONUCLEASE MUS81	Interproscan

Gene Ontology

GO term	Category	Description	Source
GO:0000712 all species →	Biological Process	resolution of meiotic recombination intermediates	Interproscan
GO:0000727 all species →	Biological Process	double-strand break repair via break-induced replication	Interproscan
GO:0005634 all species →	Cellular Component	nucleus	Interproscan
GO:0006302 all species →	Biological Process	double-strand break repair	Interproscan
GO:0006308 all species →	Biological Process	DNA catabolic process	Interproscan
GO:0008821 all species →	Molecular Function	crossover junction DNA endonuclease activity	Interproscan
GO:0031573 all species →	Biological Process	mitotic intra-S DNA damage checkpoint signaling	Interproscan
GO:0048257 all species →	Molecular Function	3'-flap endonuclease activity	Interproscan
GO:0048476 all species →	Cellular Component	Holliday junction resolvase complex	Interproscan
GO:0003677 all species →	Molecular Function	DNA binding	Interproscan
GO:0004518 all species →	Molecular Function	nuclease activity	Interproscan

Search by domain instead of by gene. Any accession above (InterPro, Pfam, PANTHER, GO, KEGG) can be used as a query on the Functional Domain Search page, which searches all 148 annotated genomes at once.

KEGG pathway

KO	Enzyme	Enzyme ID	Pathway	Map ID	Source
K08991	MUS81; crossover junction endonuclease MUS81	EC:3.1.22.-	DNA repair and recombination proteins	ko03400	deepkoala

Searching by KO or pathway ID across all species is available on the KEGG Pathway page.

Expression pattern (RNA-seq)

Transcript abundance of XP_068731291.1 across 36 RNA-seq samples of Montipora capricornis. Values are TPM (transcripts per million) from the StringTie quantification; one bar is one sample, grouped and coloured by condition, sorted by expression within each group.

36Samples

29TPM > 0

1Conditions

45.5Max TPM

18.4Mean TPM

By condition

Condition	Samples	TPM > 0	Mean TPM	Max TPM	Mean, relative to max
Polyps	36	29	18.37	45.52

Per sample · hover a bar for the full sample record

Show the sample table (36 samples)

SRA run	Condition	Tissue	Developmental stage	Treatment	Study	TPM
SRR12959227	Polyps	Polyps	E3 day3	not recorded	SRP199550	45.52
SRR12904783	Polyps	Polyps	not recorded	not recorded	SRP199550	36.49
SRR27940191	Polyps	Polyps	not recorded	not recorded	SRP199550	36.37
SRR12786896	Polyps	Polyps	OA3 day0	not recorded	SRP199550	31.00
SRR12904782	Polyps	Polyps	not recorded	not recorded	SRP199550	29.36
SRR27940193	Polyps	Polyps	not recorded	not recorded	SRP199550	29.34
SRR12959229	Polyps	Polyps	E3 day3	not recorded	SRP199550	28.52
SRR12959203	Polyps	Polyps	E3 day15	not recorded	SRP199550	28.10
SRR12904781	Polyps	Polyps	not recorded	not recorded	SRP199550	27.71
SRR12786897	Polyps	Polyps	OA3 day0	not recorded	SRP199550	26.98
SRR12959201	Polyps	Polyps	E3 day15	not recorded	SRP199550	26.98
SRR12959202	Polyps	Polyps	E3 day15	not recorded	SRP199550	26.71
SRR12959184	Polyps	Polyps	E3 day0	not recorded	SRP199550	26.30
SRR12959183	Polyps	Polyps	E3 day0	not recorded	SRP199550	26.22
SRR12959182	Polyps	Polyps	E3 day0	not recorded	SRP199550	25.75
SRR12786898	Polyps	Polyps	OA3 day0	not recorded	SRP199550	22.12
SRR12959214	Polyps	Polyps	E3 day9	not recorded	SRP199550	19.96
SRR12959216	Polyps	Polyps	E3 day9	not recorded	SRP199550	19.29
SRR12959215	Polyps	Polyps	E3 day9	not recorded	SRP199550	17.34
SRR12710858	Polyps	Polyps	OA3 day9	not recorded	SRP199550	17.32
SRR12710855	Polyps	Polyps	OA3 day9	not recorded	SRP199550	16.54
SRR27940192	Polyps	Polyps	not recorded	not recorded	SRP199550	15.65
SRR12959190	Polyps	Polyps	E3 day21	not recorded	SRP199550	15.06
SRR12959188	Polyps	Polyps	E3 day21	not recorded	SRP199550	14.95
SRR12959189	Polyps	Polyps	E3 day21	not recorded	SRP199550	12.24
SRR12710857	Polyps	Polyps	OA3 day9	not recorded	SRP199550	11.35
SRR12710847	Polyps	Polyps	OA3 day3	not recorded	SRP199550	9.66
SRR12710848	Polyps	Polyps	OA3 day3	not recorded	SRP199550	9.51
SRR12710846	Polyps	Polyps	OA3 day3	not recorded	SRP199550	8.86
SRR12807381	Polyps	Polyps	OA3 day0	not recorded	SRP199550	0.00
SRR12849113	Polyps	Polyps	OA3 day0	not recorded	SRP199550	0.00
SRR12927880	Polyps	Polyps	E3 day0	not recorded	SRP199550	0.00
SRR12959230	Polyps	Polyps	E3 day3	not recorded	SRP199550	0.00
SRR12963484	Polyps	Polyps	E3 day0	not recorded	SRP199550	0.00
SRR9129316	Polyps	Polyps	not recorded	not recorded	SRP199550	0.00
SRR9613519	Polyps	Polyps	not recorded	not recorded	SRP199550	0.00

Source: CnidoSite RNA-seq expression matrices (MCAPR_TPM, StringTie quantification over 36 runs), joined to SRA sample metadata. Samples whose tissue/treatment is not recorded in the source metadata are grouped by the descriptor carried in the expression matrix itself.

Co-expression network

Genes whose expression across the transcriptome samples of Montipora capricornis tracks this one. Counts are over the whole network; the network view itself draws at most 100 partners per query gene (CNIDO_NET_TOP_K), so a hub gene can show fewer edges than the number below.

Direction	Partners	Most correlated partner	PCC
Positively correlated	28	XP_068750470.1	0.960040034103723
Negatively correlated	3	XP_068724527.1	-0.455549765545902

Open this gene in Network Analysis → The network opens with the gene already entered and both directions selected. There you can add up to 9 more genes, switch between the positive and negative network, and export the edge list.

Single-cell expression

Whether this gene can be visualised in the single-cell atlases of Montipora capricornis, and in which cell types it is a marker. Expression is stored per cell, so the violin plot and the cell-type means are computed in the viewer — open a dataset to see them.

No single-cell dataset has been published for this species. The atlases that do exist are listed in the Cell Atlas.

Epigenetic marks

Chromatin and DNA-methylation data covering this gene. Peak calls are listed per sample with the genomic region the peak falls in; DNA methylation is listed as sample availability only, because those tables are queried by the DNA Methylation page itself.

No epigenomic data has been deposited for this species. The assays that exist across the site are described in the Epigenomic Data module.

What you can do with this gene

Every tool below opens with this gene already entered, so you land on the analysis rather than on an empty form. Links open in a new tab.

Analysis	What it does	Status
Primer design	Design PCR / qPCR primers on this gene’s sequence with the published primer3 settings. Opens with the primers already computed.	template found	open →
BLAST	Search this gene’s sequence against the CnidoSite BLAST databases. The query is filled in and the species’ database is preselected — press Run to start.	template found	open →
Expression heatmap	Draw this gene’s expression as a heatmap across all RNA-seq samples, and compare it with the genes you add next to it.	ready	open →
Gene family / orthogroup	Look this gene up in the single-copy orthogroups built from the high-quality cnidarian genomes, and see which other species carry an orthologue.		open →
Gene set analysis	Start a gene-set enrichment analysis with this gene as the seed list. Add more genes on that page before running it.		open →