Genomic Location: sc0000229_pilon:848479...877497
NR annotation: XP_029195436.2, pre-mRNA-processing-splicing factor 8 [Acropora millepora]
Species Acropora muricata · all data for this species · gene families
| CDS |
| amur_s0229.g69.t1 |
| Transcript |
| amur_s0229.g69.t1 |
| Protein |
| amur_s0229.g69.t1 |
| UniProt accession | Description |
|---|---|
| Q6P2Q9 | Pre-mRNA-processing-splicing factor 8 OS=Homo sapiens OX=9606 GN=PRPF8 PE=1 SV=2 |
| Q99PV0 | Pre-mRNA-processing-splicing factor 8 OS=Mus musculus OX=10090 GN=Prpf8 PE=1 SV=2 |
| P34369 | Pre-mRNA-splicing factor 8 homolog OS=Caenorhabditis elegans OX=6239 GN=prp-8 PE=1 SV=1 |
| Family type | Membership / link |
|---|---|
| Orthogroup (gene family) | OG0004368 (this species only) · gene tree & orthology |
| Ubiquitin family | UBD|Other|Jab_MPN · all ubiquitin genes in this species |
| Pfam accession | Pfam name | Description | Type | Source |
|---|---|---|---|---|
| PF08082 all species → | PRO8NT | PRO8NT (NUC069), PrP8 N-terminal domain | Domain | Interproscan |
| PF10598 all species → | RRM_4 | RNA recognition motif of the spliceosomal PrP8 | Domain | Interproscan |
| PF12134 all species → | PRP8_domainIV | PRP8 domain IV core | Domain | Interproscan |
| PF08083 all species → | PROCN | PROCN (NUC071) domain | Domain | Interproscan |
| PF10597 all species → | U5_2-snRNA_bdg | U5-snRNA binding site 2 of PrP8 | Domain | Interproscan |
| PF01398 all species → | JAB | JAB1/Mov34/MPN/PAD-1 ubiquitin protease | Family | Interproscan |
| PF10596 all species → | U6-snRNA_bdg | U6-snRNA interacting domain of PrP8 | Domain | Interproscan |
| PF08084 all species → | PROCT | PROCT (NUC072) domain | Domain | Interproscan |
| InterPro term | Type | Description | Source |
|---|---|---|---|
| IPR012591 all species → | Domain | PRO8NT domain | Interproscan |
| IPR019582 all species → | Domain | RNA recognition motif, spliceosomal PrP8 | Interproscan |
| IPR012337 all species → | Homologous_superfamily | Ribonuclease H-like superfamily | Interproscan |
| IPR021983 all species → | Domain | PRP8 domain IV core | Interproscan |
| IPR012592 all species → | Domain | PROCN domain | Interproscan |
| IPR019581 all species → | Domain | Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding | Interproscan |
| IPR000555 all species → | Domain | JAB1/MPN/MOV34 metalloenzyme domain | Interproscan |
| IPR019580 all species → | Domain | Pre-mRNA-processing-splicing factor 8, U6-snRNA-binding | Interproscan |
| IPR027652 all species → | Family | Pre-mRNA-processing-splicing factor 8 | Interproscan |
| IPR042516 all species → | Homologous_superfamily | Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding domain superfamily | Interproscan |
| IPR037518 all species → | Domain | MPN domain | Interproscan |
| IPR043172 all species → | Homologous_superfamily | Prp8 RNase domain IV, palm region | Interproscan |
| IPR043173 all species → | Homologous_superfamily | Prp8 RNase domain IV, fingers region | Interproscan |
| IPR012984 all species → | Domain | PROCT domain | Interproscan |
| PANTHER term | Description | Source |
|---|---|---|
| PTHR11140 all species → | PRE-MRNA SPLICING FACTOR PRP8 | Interproscan |
| GO term | Category | Description | Source |
|---|---|---|---|
| GO:0000398 all species → | Biological Process | mRNA splicing, via spliceosome | Interproscan |
| GO:0003723 all species → | Molecular Function | RNA binding | Interproscan |
| GO:0030623 all species → | Molecular Function | U5 snRNA binding | Interproscan |
| GO:0005515 all species → | Molecular Function | protein binding | Interproscan |
| GO:0008233 all species → | Molecular Function | peptidase activity | Interproscan |
| GO:0008237 all species → | Molecular Function | metallopeptidase activity | Interproscan |
| GO:0017070 all species → | Molecular Function | U6 snRNA binding | Interproscan |
| GO:0000244 all species → | Biological Process | spliceosomal tri-snRNP complex assembly | Interproscan |
| GO:0005681 all species → | Cellular Component | spliceosomal complex | Interproscan |
| GO:0005682 all species → | Cellular Component | U5 snRNP | Interproscan |
| GO:0030619 all species → | Molecular Function | U1 snRNA binding | Interproscan |
| GO:0030620 all species → | Molecular Function | U2 snRNA binding | Interproscan |
| GO:0071013 all species → | Cellular Component | catalytic step 2 spliceosome | Interproscan |
| GO:0097157 all species → | Molecular Function | pre-mRNA intronic binding | Interproscan |
| KO | Enzyme | Enzyme ID | Pathway | Map ID | Source |
|---|---|---|---|---|---|
| K12856 | PRPF8, PRP8; pre-mRNA-processing factor 8 | - | Spliceosome | ko03041 | deepkoala |
Transcript abundance of amur_s0229.g69.t1 across 82 RNA-seq samples of Acropora muricata. Values are TPM (transcripts per million) from the StringTie quantification; one bar is one sample, grouped and coloured by condition, sorted by expression within each group.
| Condition | Samples | TPM > 0 | Mean TPM | Max TPM | Mean, relative to max |
|---|---|---|---|---|---|
| Polyps | 79 | 74 | 49.08 | 359.49 | |
| Severed branch | 3 | 0 | 0.00 | 0.00 |
| SRA run | Condition | Tissue | Developmental stage | Treatment | Study | TPM |
|---|---|---|---|---|---|---|
| SRR27868191 | Polyps | Polyps | regeneration day24 | not recorded | SRP199550 | 359.49 |
| SRR12959207 | Polyps | Polyps | E2 day15 | not recorded | SRP199550 | 115.74 |
| SRR27868175 | Polyps | Polyps | regeneration day39 | not recorded | SRP199550 | 113.93 |
| SRR27868165 | Polyps | Polyps | regeneration day3 | not recorded | SRP199550 | 92.31 |
| SRR27868187 | Polyps | Polyps | regeneration day3 | not recorded | SRP199550 | 85.40 |
| SRR27868201 | Polyps | Polyps | regeneration day15 | not recorded | SRP199550 | 81.98 |
| SRR27940225 | Polyps | Polyps | not recorded | not recorded | SRP199550 | 81.77 |
| SRR27868192 | Polyps | Polyps | regeneration day24 | not recorded | SRP199550 | 80.32 |
| SRR27868176 | Polyps | Polyps | regeneration day3 | not recorded | SRP199550 | 79.62 |
| SRR27868199 | Polyps | Polyps | regeneration day18 | not recorded | SRP199550 | 76.02 |
| SRR27868189 | Polyps | Polyps | regeneration day27 | not recorded | SRP199550 | 75.39 |
| SRR12959205 | Polyps | Polyps | E2 day15 | not recorded | SRP199550 | 74.68 |
| SRR12959204 | Polyps | Polyps | E2 day15 | not recorded | SRP199550 | 73.87 |
| SRR27868188 | Polyps | Polyps | regeneration day27 | not recorded | SRP199550 | 73.49 |
| SRR27868183 | Polyps | Polyps | regeneration day33 | not recorded | SRP199550 | 72.54 |
| SRR27868186 | Polyps | Polyps | regeneration day30 | not recorded | SRP199550 | 69.90 |
| SRR27868193 | Polyps | Polyps | regeneration day24 | not recorded | SRP199550 | 69.73 |
| SRR27868185 | Polyps | Polyps | regeneration day30 | not recorded | SRP199550 | 68.77 |
| SRR27868197 | Polyps | Polyps | regeneration day18 | not recorded | SRP199550 | 66.66 |
| SRR12959206 | Polyps | Polyps | E2 day0 | not recorded | SRP199550 | 66.02 |
| SRR27868200 | Polyps | Polyps | regeneration day18 | not recorded | SRP199550 | 65.04 |
| SRR27868205 | Polyps | Polyps | regeneration day12 | not recorded | SRP199550 | 64.29 |
| SRR27868182 | Polyps | Polyps | regeneration day33 | not recorded | SRP199550 | 63.71 |
| SRR27868203 | Polyps | Polyps | regeneration day15 | not recorded | SRP199550 | 63.47 |
| SRR27868190 | Polyps | Polyps | regeneration day27 | not recorded | SRP199550 | 63.22 |
| SRR27868180 | Polyps | Polyps | regeneration day36 | not recorded | SRP199550 | 61.98 |
| SRR12959195 | Polyps | Polyps | E2 day0 | not recorded | SRP199550 | 59.28 |
| SRR27868207 | Polyps | Polyps | regeneration day9 | not recorded | SRP199550 | 58.96 |
| SRR27868208 | Polyps | Polyps | regeneration day9 | not recorded | SRP199550 | 58.05 |
| SRR27868184 | Polyps | Polyps | regeneration day30 | not recorded | SRP199550 | 57.50 |
| SRR27868202 | Polyps | Polyps | regeneration day15 | not recorded | SRP199550 | 56.44 |
| SRR12959217 | Polyps | Polyps | E2 day0 | not recorded | SRP199550 | 55.99 |
| SRR27868174 | Polyps | Polyps | regeneration day39 | not recorded | SRP199550 | 55.25 |
| SRR27868160 | Polyps | Polyps | regeneration day6 | not recorded | SRP199550 | 49.83 |
| SRR27868179 | Polyps | Polyps | regeneration day36 | not recorded | SRP199550 | 48.82 |
| SRR12904785 | Polyps | Polyps | not recorded | not recorded | SRP199550 | 45.86 |
| SRR27868209 | Polyps | Polyps | regeneration day0 | not recorded | SRP199550 | 45.73 |
| SRR12959232 | Polyps | Polyps | E2 day3 | not recorded | SRP199550 | 42.92 |
| SRR27868194 | Polyps | Polyps | regeneration day21 | not recorded | SRP199550 | 41.60 |
| SRR27868158 | Polyps | Polyps | regeneration day6 | not recorded | SRP199550 | 41.50 |
| SRR27868181 | Polyps | Polyps | regeneration day33 | not recorded | SRP199550 | 41.22 |
| SRR27868204 | Polyps | Polyps | regeneration day12 | not recorded | SRP199550 | 41.03 |
| SRR12959220 | Polyps | Polyps | E2 day9 | not recorded | SRP199550 | 40.84 |
| SRR12904784 | Polyps | Polyps | not recorded | not recorded | SRP199550 | 40.75 |
| SRR27940224 | Polyps | Polyps | not recorded | not recorded | SRP199550 | 40.74 |
| SRR27868198 | Polyps | Polyps | regeneration day0 | not recorded | SRP199550 | 39.73 |
| SRR12959218 | Polyps | Polyps | E2 day9 | not recorded | SRP199550 | 39.21 |
| SRR27868206 | Polyps | Polyps | regeneration day12 | not recorded | SRP199550 | 37.36 |
| SRR27940226 | Polyps | Polyps | not recorded | not recorded | SRP199550 | 37.10 |
| SRR12959219 | Polyps | Polyps | E2 day9 | not recorded | SRP199550 | 37.06 |
| SRR12959233 | Polyps | Polyps | E2 day3 | not recorded | SRP199550 | 35.52 |
| SRR12959231 | Polyps | Polyps | E2 day3 | not recorded | SRP199550 | 35.13 |
| SRR12959191 | Polyps | polyps | E2 day21 | not recorded | SRP199550 | 31.63 |
| SRR12959192 | Polyps | Polyps | E2 day21 | not recorded | SRP199550 | 31.23 |
| SRR27868178 | Polyps | Polyps | regeneration day36 | not recorded | SRP199550 | 31.08 |
| SRR12959193 | Polyps | Polyps | E2 day21 | not recorded | SRP199550 | 29.51 |
| SRR27868177 | Polyps | Polyps | regeneration day39 | not recorded | SRP199550 | 27.28 |
| SRR27868159 | Polyps | Polyps | regeneration day6 | not recorded | SRP199550 | 24.84 |
| SRR12786900 | Polyps | Polyps | OA 2 day0 | not recorded | SRP199550 | 24.33 |
| SRR12786899 | Polyps | Polyps | OA 2 day0 | not recorded | SRP199550 | 22.73 |
| SRR12786901 | Polyps | Polyps | OA 2 day0 | not recorded | SRP199550 | 20.76 |
| SRR27868195 | Polyps | Polyps | regeneration day21 | not recorded | SRP199550 | 18.95 |
| SRR27940222 | Polyps | Polyps | not recorded | not recorded | SRP199550 | 18.24 |
| SRR27868210 | Polyps | Polyps | regeneration day0 | not recorded | SRP199550 | 17.55 |
| SRR27940228 | Polyps | Polyps | not recorded | not recorded | SRP199550 | 17.54 |
| SRR12904786 | Polyps | Polyps | not recorded | not recorded | SRP199550 | 17.45 |
| SRR12710849 | Polyps | Polyps | OA 2 day3 | not recorded | SRP199550 | 16.45 |
| SRR12710850 | Polyps | Polyps | OA 2 day3 | not recorded | SRP199550 | 15.55 |
| SRR27868196 | Polyps | Polyps | regeneration day21 | not recorded | SRP199550 | 14.62 |
| SRR12710851 | Polyps | Polyps | OA 2 day3 | not recorded | SRP199550 | 14.42 |
| SRR12710860 | Polyps | Polyps | OA 2 day9 | not recorded | SRP199550 | 11.47 |
| SRR12710859 | Polyps | Polyps | OA 2 day9 | not recorded | SRP199550 | 10.24 |
| SRR12710861 | Polyps | Polyps | OA 2 day9 | not recorded | SRP199550 | 9.86 |
| SRR27940227 | Polyps | Polyps | not recorded | not recorded | SRP199550 | 8.93 |
| SRR12807382 | Polyps | Polyps | OA2 day0 | not recorded | SRP199550 | 0.00 |
| SRR12927881 | Polyps | Polyps | E2 day0 | not recorded | SRP199550 | 0.00 |
| SRR27940229 | Polyps | Polyps | not recorded | not recorded | SRP199550 | 0.00 |
| SRR9613488 | Polyps | Polyps | not recorded | not recorded | SRP199550 | 0.00 |
| SRR9613516 | Polyps | Polyps | not recorded | not recorded | SRP199550 | 0.00 |
| SRR12995717 | Severed branch | Severed branch | regeneration day0 | not recorded | SRP199550 | 0.00 |
| SRR12996627 | Severed branch | Severed branch | regeneration day0 | not recorded | SRP199550 | 0.00 |
| SRR12996628 | Severed branch | Severed branch | regeneration High gene expression | not recorded | SRP199550 | 0.00 |
Source: CnidoSite RNA-seq expression matrices (AMURI_TPM,
StringTie quantification over 82 runs), joined to SRA sample
metadata. Samples whose tissue/treatment is not recorded in the source metadata are grouped
by the descriptor carried in the expression matrix itself.
Genes whose expression across the transcriptome samples of Acropora muricata tracks this one. Counts are over the whole network; the network view itself draws at most 100 partners per query gene (CNIDO_NET_TOP_K), so a hub gene can show fewer edges than the number below.
| Direction | Partners | Most correlated partner | PCC |
|---|---|---|---|
| Positively correlated | 46 | amur_s0029.g40.t1 | 0.941280730369269 |
| Negatively correlated | 14 | amur_s2201.g1.t1 | -0.331487537212623 |
Open this gene in Network Analysis → The network opens with the gene already entered and both directions selected. There you can add up to 9 more genes, switch between the positive and negative network, and export the edge list.
Whether this gene can be visualised in the single-cell atlases of Acropora muricata, and in which cell types it is a marker. Expression is stored per cell, so the violin plot and the cell-type means are computed in the viewer — open a dataset to see them.
| Dataset | Tissue / stage | Cells | Cell types | This gene | Marker of |
|---|---|---|---|---|---|
| AMURI_regen | Polyps · regeneration | 8,721 | 28 | unmapped | – |
A gene is in viewer when the dataset ships a per-cell expression vector for it; marker only means it is in the dataset’s ranked marker table but no vector was exported, so the atlas cannot draw it; not in this dataset means the dataset’s own gene-ID table does not list it, so no expression claim can be made for that dataset. unmapped is deliberately weaker still: the dataset has no gene-ID table on CnidoSite yet, so nothing is claimed in either direction. Where markers are listed, the numbers are log2 fold change and the percentage of cells of that type in which the gene was detected.
Chromatin and DNA-methylation data covering this gene. Peak calls are listed per sample with the genomic region the peak falls in; DNA methylation is listed as sample availability only, because those tables are queried by the DNA Methylation page itself.
No epigenomic data has been deposited for this species. The assays that exist across the site are described in the Epigenomic Data module.
Every tool below opens with this gene already entered, so you land on the analysis rather than on an empty form. Links open in a new tab.
| Analysis | What it does | Status | |
|---|---|---|---|
| Primer design | Design PCR / qPCR primers on this gene’s sequence with the published primer3 settings. Opens with the primers already computed. | template found | open → |
| BLAST | Search this gene’s sequence against the CnidoSite BLAST databases. The query is filled in and the species’ database is preselected — press Run to start. | template found | open → |
| Expression heatmap | Draw this gene’s expression as a heatmap across all RNA-seq samples, and compare it with the genes you add next to it. | ready | open → |
| Gene family / orthogroup | Look this gene up in the single-copy orthogroups built from the high-quality cnidarian genomes, and see which other species carry an orthologue. | open → | |
| Gene set analysis | Start a gene-set enrichment analysis with this gene as the seed list. Add more genes on that page before running it. | open → |