Gene detail

Detailed information of g2422.t1 in Acropora digitifera

Genomic Location: chr1Alt:37686068...37700315
NR annotation: XP_044180592.1, guanine nucleotide exchange factor DBS-like isoform X3 [Acropora millepora]
Species Acropora digitifera · all data for this species · gene families

Gene Structure

More details in JBrowse

Sequence

CDS

g2422.t1

ATGCGTAGGACATTAGGTAGACTGTCCATTTTCGCACCCGGAGCAAGCAATGAAGAACCAAAGATCAAGGCCATTGAAATTGCTGCATTGTTGCAAGAAAAATATGCCTTCCTTTCTGGAGCACAAGCTAAAAATGGCTGTCTCATCATCACAATGCCTGAACATCCGAAGTTTGGAAGCCTCAGTGACAGAGATGCTGAACGTATTTTCAGATATCTCGTTGGATTAGCCAGAGAAGACCTCCTTCACAGAGGCTTTATGTTGGTGCTTGATAGGAGGAGAGAAAAATGGGGAACTGTGAAATCCTGTCTTAACAAAGTTCAGAAATGCTTCCCAGCAAAAATCAGCCTTGTCTGTTTGATTAAACCTCAAGGCTTTTTGCAAAAATATTCCAAACCAGGCTTTGTAACGGATGGAATCATAGGAGATGCAAAAATACAGGTTGTTTCTCTTAATTCTGTTACTGAACTGGATGGATACATTGAAAAATCCCAGCTAACAGCAGACGTGGGAGGGACTTTAGATTACAGGCATAGTGAATGGGTGCAGTATAGATCAGCAATTGAAAGGTTTCAGAGGAACTTGCAAAACTCAAGTGACAGAATTCAAGAATTGGAACAAGGATTCACAAGAACAAGATTGTCACCAAGTGTCCAGGAAATAGAACACATTTTAGAATCACAGGAAGAAATGTGGCATGATGTTAAGGAGGATATAGAAAGCTCCAGACAAGCTGGAGTAACATTATTGGACTGTATAACTGTGCATCGCAGAAATAAAGAAAACACTGAAAGGGTCCTTACACCAGATATCAAAGCAAATGCTGCAGAACTCAAGGAAGCTCTAACTGAACTTGAGTCTTTGGAAAATAATTTTGAAAAGTTTTGGAAGTTTCACAAGTTGAAGATACAATATGGGCTGAAAGTGTGTTTGTTTGAGCGGGAAATGAGTCAGACAACAGGGCTTCTCCTGTCAGCACTGGGTAATCTGTCAGCCATGGCAGATTTTGGAGACTCAAGAGATGATGCAGAATCATTATTGCAAGACCTCAAAGAACTTGCAGAGAAATCTGTGGAACTCCTAGATAAAGCAAAAAGTTTAATTGCCAAGGGAACAAGGATGATAGAGAGCAACGAACAGTCAAAAGATCAAATCCAGCTGAAGTGTGATGAAACCAGAGAACTTTGTGATAAGTTTGAGAGAGAAGTACAGAAGAGAGAAGAGGATTTGAAAAAAGCAATGGATATTCATGAATGTCTGGAAATGGTGAATAAGTGGTGCAAGACCGGTGTGGATCTGCTAGCAACCCAACCCATAGACAGGTTCCAGTCACCAGGAGGTGCAGAGAAAGCATTGGAGGAAATTGAGAACTTCTTGAAGAGCCCACAAGGAATGAACATGGCCAAGGTCAGGAAGATGACTCCAATGAGCAATGCACTGGGCAATAAAACACTGATCAGCAAAGTAAAGGGTAGTCACAAGAGGATCAGTGAAGTCAATGAGATGTTGGCAAAACGAGAAACCAGTTTGAAAAAACTTGTTGTTAAGCGGCCAGTGCAACCTGTGTCTGGTATACCAGCCAATCCTCCAAAACAAGAGGAAATAAAACGCAAACACAGTATAACACCAAAAAGTCCCAGCACACCGAAGAGTCCAGTCATAGTTTACAAAGAACGCAAGAAATCTATTGCAGCTGAATCAACTGGCAAGAGACCTGTGTCTATTGTTTTAGAACCAGACTCCTTTCAGAAATCTGGAGGCATACAGCAGTCTCTCTCTGAACCATCTATGCAGACGAGAGATGTTGTGGATGGTACTAATGGTCCAGAGGCTCGTAAGAAGAGAGAACAAGTTATGACAGAGTTAATTGAAACAGAGAAAGACTATGTTGATGATTTGGAGTGCATCATTAATGGTTACATAAAACAGTTTGAACAAGCAGGAAACAAAATTCCTGCTGAGTTACATGGCAAGAAGACGGTTATATTTGGTAACATTGGACAGATTTATAGATTTCACCGTAATGAATTTCTGTGTGAACTAGAGGCCAATAAGGATCAGCCATCACAGGTTGGGGGATTATTTTTCACAAAGAGCACTGAATTTGAAATGTATGCAACTTATTGCAAGAATCAGCCAAGTTCTGAAGCTCTCAGAATGCATTACATCAATTTGCCTTTCTTCAAGGAATGTCAAGATAAGCTTGGCCACAAACTTCCACTGAGTGCTTACCTTCTTAAACCAGTGCAGAGAATAACAAAGTACCAGCTTCTCATCAGAGAAATGATGAAACTAACCGTTAAAAATAGTACTGCATTTGATGATCTGGAGAAAGCCCTTGAAACAATGGAAGGAGTCTTACGGCATGTCAATGATGTCATGCACTCTTGTGGAATAAAGGGTTTCCGTGGTAACTTGGCCGAACAGGGAAAGCTTCTTCTCCAAGACACATTCATGGTTTGGGAGGCAAATAGAAACTGGAATTTAAATTTGTTACCTTCAAAGGGAGGAAGGCAGCGTCAGGTGTTCATGTATCAAAAGATGATTATTTTTAGCAAAAAGGATGATGATACCAGCAGAGATTCAGTCACTTATCAGTACAAGAAACATATAAAGACCTGTGACATGGCATTCACAGAGACTGTGCGAGGTGATCCACTTAAGTTTGAACTGTCGCTGACAGGACGATCAGAAGTGTTTGTTATGCAACCTTCTTCAACAGCTGTCAAGAACCGCTGGGTTAAAGAAATAAGAAATTTGATGTGGTCGCAGTTTGATGAAGTGAAAGAAGCTGTCTCAACAGAACAAACTGGACTTCACAGTCTCTGGAAGTCAGCTTCAATGTTGTCCTCTGCCACAAAGAGTTCGGAAAATGGGCTTCGCTCAAGAAGCCAATCGTCACTTTCGGTGGTTGGACCAAACATAGTGGGTGGTAATAAGAGGGTTTCAAACATTCAAGAGTTTGATGATGATGATGAAGGATGTGATACTGATGAGTGGGATTCTTCGGATAGCAGTGATGATTGTGCTACACAACAACAGCAAACAAAATCTGAAAACATGATTAATATCAGGCAAGATCTGCCAAGTAAAGGTTCACGATACTCTGCTATGGCCAGCTACAAAGCTTTGGAAAGTACAGAGCTGTCATTCCAACAGGGAGACATCATGGAACTGTTGCGAGTAGGACAAGATGGCTGGTGGCATACACGACACACCGCAACAAAAGAAGAGGGATGGGTTCCAGCTAGTTTTTTGGAATTGCTGCAATCATAG

Transcript

chr1Alt.g2422.t1

TCGCCATGTTGGTTTTGTTTTGCATATATTTTCCATCATGAGGAAAGTGTAGGGAAATGAAATTTTTATCGTGAAATAATAAGCACATCATGCGTAGGACATTAGGTAGACTGTCCATTTTCGCACCCGGAGCAAGCAATGAAGAACCAAAGATCAAGGCCATTGAAATTGCTGCATTGTTGCAAGAAAAATATGCCTTCCTTTCTGGAGCACAAGCTAAAAATGGCTGTCTCATCATCACAATGCCTGAACATCCGAAGTTTGGAAGCCTCAGTGACAGAGATGCTGAACGTATTTTCAGATATCTCGTTGGATTAGCCAGAGAAGACCTCCTTCACAGAGGCTTTATGTTGGTGCTTGATAGGAGGAGAGAAAAATGGGGAACTGTGAAATCCTGTCTTAACAAAGTTCAGAAATGCTTCCCAGCAAAAATCAGCCTTGTCTGTTTGATTAAACCTCAAGGCTTTTTGCAAAAATATTCCAAACCAGGCTTTGTAACGGATGGAATCATAGGAGATGCAAAAATACAGGTTGTTTCTCTTAATTCTGTTACTGAACTGGATGGATACATTGAAAAATCCCAGCTAACAGCAGACGTGGGAGGGACTTTAGATTACAGGCATAGTGAATGGGTGCAGTATAGATCAGCAATTGAAAGGTTTCAGAGGAACTTGCAAAACTCAAGTGACAGAATTCAAGAATTGGAACAAGGATTCACAAGAACAAGATTGTCACCAAGTGTCCAGGAAATAGAACACATTTTAGAATCACAGGAAGAAATGTGGCATGATGTTAAGGAGGATATAGAAAGCTCCAGACAAGCTGGAGTAACATTATTGGACTGTATAACTGTGCATCGCAGAAATAAAGAAAACACTGAAAGGGTCCTTACACCAGATATCAAAGCAAATGCTGCAGAACTCAAGGAAGCTCTAACTGAACTTGAGTCTTTGGAAAATAATTTTGAAAAGTTTTGGAAGTTTCACAAGTTGAAGATACAATATGGGCTGAAAGTGTGTTTGTTTGAGCGGGAAATGAGTCAGACAACAGGGCTTCTCCTGTCAGCACTGGGTAATCTGTCAGCCATGGCAGATTTTGGAGACTCAAGAGATGATGCAGAATCATTATTGCAAGACCTCAAAGAACTTGCAGAGAAATCTGTGGAACTCCTAGATAAAGCAAAAAGTTTAATTGCCAAGGGAACAAGGATGATAGAGAGCAACGAACAGTCAAAAGATCAAATCCAGCTGAAGTGTGATGAAACCAGAGAACTTTGTGATAAGTTTGAGAGAGAAGTACAGAAGAGAGAAGAGGATTTGAAAAAAGCAATGGATATTCATGAATGTCTGGAAATGGTGAATAAGTGGTGCAAGACCGGTGTGGATCTGCTAGCAACCCAACCCATAGACAGGTTCCAGTCACCAGGAGGTGCAGAGAAAGCATTGGAGGAAATTGAGAACTTCTTGAAGAGCCCACAAGGAATGAACATGGCCAAGGTCAGGAAGATGACTCCAATGAGCAATGCACTGGGCAATAAAACACTGATCAGCAAAGTAAAGGGTAGTCACAAGAGGATCAGTGAAGTCAATGAGATGTTGGCAAAACGAGAAACCAGTTTGAAAAAACTTGTTGTTAAGCGGCCAGTGCAACCTGTGTCTGGTATACCAGCCAATCCTCCAAAACAAGAGGAAATAAAACGCAAACACAGTATAACACCAAAAAGTCCCAGCACACCGAAGAGTCCAGTCATAGTTTACAAAGAACGCAAGAAATCTATTGCAGCTGAATCAACTGGCAAGAGACCTGTGTCTATTGTTTTAGAACCAGACTCCTTTCAGAAATCTGGAGGCATACAGCAGTCTCTCTCTGAACCATCTATGCAGACGAGAGATGTTGTGGATGGTACTAATGGTCCAGAGGCTCGTAAGAAGAGAGAACAAGTTATGACAGAGTTAATTGAAACAGAGAAAGACTATGTTGATGATTTGGAGTGCATCATTAATGGTTACATAAAACAGTTTGAACAAGCAGGAAACAAAATTCCTGCTGAGTTACATGGCAAGAAGACGGTTATATTTGGTAACATTGGACAGATTTATAGATTTCACCGTAATGAATTTCTGTGTGAACTAGAGGCCAATAAGGATCAGCCATCACAGGTTGGGGGATTATTTTTCACAAAGAGCACTGAATTTGAAATGTATGCAACTTATTGCAAGAATCAGCCAAGTTCTGAAGCTCTCAGAATGCATTACATCAATTTGCCTTTCTTCAAGGAATGTCAAGATAAGCTTGGCCACAAACTTCCACTGAGTGCTTACCTTCTTAAACCAGTGCAGAGAATAACAAAGTACCAGCTTCTCATCAGAGAAATGATGAAACTAACCGTTAAAAATAGTACTGCATTTGATGATCTGGAGAAAGCCCTTGAAACAATGGAAGGAGTCTTACGGCATGTCAATGATGTCATGCACTCTTGTGGAATAAAGGGTTTCCGTGGTAACTTGGCCGAACAGGGAAAGCTTCTTCTCCAAGACACATTCATGGTTTGGGAGGCAAATAGAAACTGGAATTTAAATTTGTTACCTTCAAAGGGAGGAAGGCAGCGTCAGGTGTTCATGTATCAAAAGATGATTATTTTTAGCAAAAAGGATGATGATACCAGCAGAGATTCAGTCACTTATCAGTACAAGAAACATATAAAGACCTGTGACATGGCATTCACAGAGACTGTGCGAGGTGATCCACTTAAGTTTGAACTGTCGCTGACAGGACGATCAGAAGTGTTTGTTATGCAACCTTCTTCAACAGCTGTCAAGAACCGCTGGGTTAAAGAAATAAGAAATTTGATGTGGTCGCAGTTTGATGAAGTGAAAGAAGCTGTCTCAACAGAACAAACTGGACTTCACAGTCTCTGGAAGTCAGCTTCAATGTTGTCCTCTGCCACAAAGAGTTCGGAAAATGGGCTTCGCTCAAGAAGCCAATCGTCACTTTCGGTGGTTGGACCAAACATAGTGGGTGGTAATAAGAGGGTTTCAAACATTCAAGAGTTTGATGATGATGATGAAGGATGTGATACTGATGAGTGGGATTCTTCGGATAGCAGTGATGATTGTGCTACACAACAACAGCAAACAAAATCTGAAAACATGATTAATATCAGGCAAGATCTGCCAAGTAAAGGTTCACGATACTCTGCTATGGCCAGCTACAAAGCTTTGGAAAGTACAGAGCTGTCATTCCAACAGGGAGACATCATGGAACTGTTGCGAGTAGGACAAGATGGCTGGTGGCATACACGACACACCGCAACAAAAGAAGAGGGATGGGTTCCAGCTAGTTTTTTGGAATTGCTGCAATCATAGGGTCTCCTGTGATTACATTCAAACCTCGATTAGCTGAACTCGTGGGTAATCAAAATATGACCATCAG

Protein

UniProt (Swiss-Prot top hit)

UniProt accession	Description
Q63406	Guanine nucleotide exchange factor DBS OS=Rattus norvegicus OX=10116 GN=Mcf2l PE=1 SV=3
Q64096	Guanine nucleotide exchange factor DBS OS=Mus musculus OX=10090 GN=Mcf2l PE=1 SV=2
O15068	Guanine nucleotide exchange factor DBS OS=Homo sapiens OX=9606 GN=MCF2L PE=1 SV=2

Pfam domain

Pfam accession	Pfam name	Description	Type	Source
PF13716 all species →	CRAL_TRIO_2	Divergent CRAL/TRIO domain	Domain	Interproscan
PF00621 all species →	RhoGEF	RhoGEF domain	Domain	Interproscan
PF00018 all species →	SH3_1	SH3 domain	Domain	Interproscan

InterPro

InterPro term	Type	Description	Source
IPR000219 all species →	Domain	Dbl homology (DH) domain	Interproscan
IPR011993 all species →	Homologous_superfamily	PH-like domain superfamily	Interproscan
IPR001251 all species →	Domain	CRAL-TRIO lipid binding domain	Interproscan
IPR035899 all species →	Homologous_superfamily	Dbl homology (DH) domain superfamily	Interproscan
IPR018159 all species →	Repeat	Spectrin/alpha-actinin	Interproscan
IPR001452 all species →	Domain	SH3 domain	Interproscan
IPR036028 all species →	Homologous_superfamily	SH3-like domain superfamily	Interproscan
IPR001849 all species →	Domain	Pleckstrin homology domain	Interproscan
IPR001331 all species →	Conserved_site	Guanine-nucleotide dissociation stimulator, CDC24, conserved site	Interproscan
IPR051336 all species →	Family	Rho GTPase-activating Guanine Nucleotide Exchange Factors	Interproscan

PANTHER

PANTHER term	Description	Source
PTHR22826 all species →	RHO GUANINE EXCHANGE FACTOR-RELATED	Interproscan

Gene Ontology

GO term	Category	Description	Source
GO:0005085 all species →	Molecular Function	guanyl-nucleotide exchange factor activity	Interproscan
GO:0005515 all species →	Molecular Function	protein binding	Interproscan
GO:0035556 all species →	Biological Process	intracellular signal transduction	Interproscan
GO:0005737 all species →	Cellular Component	cytoplasm	Interproscan

Search by domain instead of by gene. Any accession above (InterPro, Pfam, PANTHER, GO, KEGG) can be used as a query on the Functional Domain Search page, which searches all 148 annotated genomes at once.

KEGG pathway

KO	Enzyme	Enzyme ID	Pathway	Map ID	Source
K20685	MCF2, ARHGEF14_21_22; guanine nucleotide exchange factor MCF2	-	Membrane trafficking	ko04131	deepkoala

Searching by KO or pathway ID across all species is available on the KEGG Pathway page.

Expression pattern (RNA-seq)

Transcript abundance of g2422.t1 across 39 RNA-seq samples of Acropora digitifera. Values are TPM (transcripts per million) from the StringTie quantification; one bar is one sample, grouped and coloured by condition, sorted by expression within each group.

39Samples

39TPM > 0

1Conditions

69.8Max TPM

40.4Mean TPM

By condition

Condition	Samples	TPM > 0	Mean TPM	Max TPM	Mean, relative to max
Coral branch	39	39	40.41	69.82

Per sample · hover a bar for the full sample record

Show the sample table (39 samples)

SRA run	Condition	Tissue	Developmental stage	Treatment	Study	TPM
SRR23047210	Coral branch	Coral branch	adult	not recorded	SRP416931	69.82
SRR23047208	Coral branch	Coral branch	adult	not recorded	SRP416931	69.37
SRR23047222	Coral branch	Coral branch	adult	not recorded	SRP416931	63.19
SRR23047219	Coral branch	Coral branch	adult	not recorded	SRP416931	55.81
SRR23047209	Coral branch	Coral branch	adult	not recorded	SRP416931	52.24
SRR23047232	Coral branch	Coral branch	adult	not recorded	SRP416931	49.48
SRR23047243	Coral branch	Coral branch	adult	not recorded	SRP416931	49.43
SRR23047242	Coral branch	Coral branch	adult	not recorded	SRP416931	47.53
SRR23047206	Coral branch	Coral branch	adult	not recorded	SRP416931	45.57
SRR23047227	Coral branch	Coral branch	adult	not recorded	SRP416931	43.19
SRR23047234	Coral branch	Coral branch	adult	not recorded	SRP416931	43.11
SRR23047211	Coral branch	Coral branch	adult	not recorded	SRP416931	42.92
SRR23047240	Coral branch	Coral branch	adult	not recorded	SRP416931	42.76
SRR23047237	Coral branch	Coral branch	adult	not recorded	SRP416931	42.46
SRR23047220	Coral branch	Coral branch	adult	not recorded	SRP416931	41.64
SRR23047233	Coral branch	Coral branch	adult	not recorded	SRP416931	41.63
SRR23047214	Coral branch	Coral branch	adult	not recorded	SRP416931	40.72
SRR23047223	Coral branch	Coral branch	adult	not recorded	SRP416931	39.88
SRR23047236	Coral branch	Coral branch	adult	not recorded	SRP416931	38.10
SRR23047239	Coral branch	Coral branch	adult	not recorded	SRP416931	37.83
SRR23047241	Coral branch	Coral branch	adult	not recorded	SRP416931	37.55
SRR23047226	Coral branch	Coral branch	adult	not recorded	SRP416931	36.43
SRR23047224	Coral branch	Coral branch	adult	not recorded	SRP416931	36.35
SRR23047213	Coral branch	Coral branch	adult	not recorded	SRP416931	34.92
SRR23047216	Coral branch	Coral branch	adult	not recorded	SRP416931	34.45
SRR23047231	Coral branch	Coral branch	adult	not recorded	SRP416931	34.19
SRR23047229	Coral branch	Coral branch	adult	not recorded	SRP416931	33.82
SRR23047221	Coral branch	Coral branch	adult	not recorded	SRP416931	33.71
SRR23047244	Coral branch	Coral branch	adult	not recorded	SRP416931	33.18
SRR23047238	Coral branch	Coral branch	adult	not recorded	SRP416931	32.91
SRR23047235	Coral branch	Coral branch	adult	not recorded	SRP416931	32.86
SRR23047215	Coral branch	Coral branch	adult	not recorded	SRP416931	32.52
SRR23047217	Coral branch	Coral branch	adult	not recorded	SRP416931	32.07
SRR23047218	Coral branch	Coral branch	adult	not recorded	SRP416931	31.89
SRR23047207	Coral branch	Coral branch	adult	not recorded	SRP416931	30.02
SRR23047225	Coral branch	Coral branch	adult	not recorded	SRP416931	29.81
SRR23047228	Coral branch	Coral branch	adult	not recorded	SRP416931	28.96
SRR23047230	Coral branch	Coral branch	adult	not recorded	SRP416931	28.75
SRR23047212	Coral branch	Coral branch	adult	not recorded	SRP416931	24.82

Source: CnidoSite RNA-seq expression matrices (ADIGI_TPM, StringTie quantification over 39 runs), joined to SRA sample metadata. Samples whose tissue/treatment is not recorded in the source metadata are grouped by the descriptor carried in the expression matrix itself.

Co-expression network

Genes whose expression across the transcriptome samples of Acropora digitifera tracks this one. Counts are over the whole network; the network view itself draws at most 100 partners per query gene (CNIDO_NET_TOP_K), so a hub gene can show fewer edges than the number below.

Direction	Partners	Most correlated partner	PCC
Positively correlated	34	g14469.t1	0.856372750812534
Negatively correlated	10	g28346.t1	-0.779398177089875

Open this gene in Network Analysis → The network opens with the gene already entered and both directions selected. There you can add up to 9 more genes, switch between the positive and negative network, and export the edge list.

Single-cell expression

Whether this gene can be visualised in the single-cell atlases of Acropora digitifera, and in which cell types it is a marker. Expression is stored per cell, so the violin plot and the cell-type means are computed in the viewer — open a dataset to see them.

No single-cell dataset has been published for this species. The atlases that do exist are listed in the Cell Atlas.

Epigenetic marks

Chromatin and DNA-methylation data covering this gene. Peak calls are listed per sample with the genomic region the peak falls in; DNA methylation is listed as sample availability only, because those tables are queried by the DNA Methylation page itself.

Peak calls overlapping this gene

Assay	Sample	Peaks	Region

No called peak overlaps this gene in 1 available assay. Either the gene is not near an accessible or marked region in those samples, or it is not represented in the peak caller’s annotation.

Browse the full epigenomic landscape of this species: DNase-seq (DHS).

What you can do with this gene

Every tool below opens with this gene already entered, so you land on the analysis rather than on an empty form. Links open in a new tab.

Analysis	What it does	Status
Primer design	Design PCR / qPCR primers on this gene’s sequence with the published primer3 settings. Opens with the primers already computed.	template found	open →
BLAST	Search this gene’s sequence against the CnidoSite BLAST databases. The query is filled in and the species’ database is preselected — press Run to start.	template found	open →
Expression heatmap	Draw this gene’s expression as a heatmap across all RNA-seq samples, and compare it with the genes you add next to it.	ready	open →
Gene family / orthogroup	Look this gene up in the single-copy orthogroups built from the high-quality cnidarian genomes, and see which other species carry an orthologue.		open →
Gene set analysis	Start a gene-set enrichment analysis with this gene as the seed list. Add more genes on that page before running it.		open →