Detailed information of HSymV2.0_g02.02262_t1 in Hydractinia symbiolongicarpus

Genomic Location: HiC_scaffold_2:504755...506725
NR annotation: MBQ4874599.1, NAD-dependent DNA ligase LigA [Rickettsiaceae bacterium H1]
Species Hydractinia symbiolongicarpus · all data for this species · gene families


 Gene Structure
More details in JBrowse  Sequence
CDS
Transcript
Protein
 UniProt (Swiss-Prot top hit)
UniProt accessionDescription
Q2GHG1DNA ligase OS=Ehrlichia chaffeensis (strain ATCC CRL-10679 / Arkansas) OX=205920 GN=ligA PE=3 SV=1
Q3YRC1DNA ligase OS=Ehrlichia canis (strain Jake) OX=269484 GN=ligA PE=3 SV=1
Q2GDR2DNA ligase OS=Neorickettsia sennetsu (strain ATCC VR-367 / Miyayama) OX=222891 GN=ligA PE=3 SV=1
 Gene family
Family typeMembership / link
Orthogroup (gene family)OG0011298 (this species only) · gene tree & orthology

 Pfam domain
Pfam accessionPfam nameDescriptionTypeSource
PF01653
all species →
DNA_ligase_adenNAD-dependent DNA ligase adenylation domainDomainInterproscan
PF03120
all species →
DNA_ligase_OBNAD-dependent DNA ligase OB-fold domainDomainInterproscan
PF14520
all species →
HHH_5Helix-hairpin-helix domainDomainInterproscan
PF00533
all species →
BRCTBRCA1 C Terminus (BRCT) domainFamilyInterproscan
PF12826
all species →
HHH_2Helix-hairpin-helix motifMotifInterproscan

 InterPro
InterPro termTypeDescriptionSource
IPR013839
all species →
DomainNAD-dependent DNA ligase, adenylationInterproscan
IPR013840
all species →
DomainNAD-dependent DNA ligase, N-terminalInterproscan
IPR036420
all species →
Homologous_superfamilyBRCT domain superfamilyInterproscan
IPR001357
all species →
DomainBRCT domainInterproscan
IPR001679
all species →
FamilyNAD-dependent DNA ligaseInterproscan
IPR010994
all species →
Homologous_superfamilyRuvA domain 2-likeInterproscan
IPR004150
all species →
DomainNAD-dependent DNA ligase, OB-foldInterproscan
IPR012340
all species →
Homologous_superfamilyNucleic acid-binding, OB-foldInterproscan
IPR041663
all species →
DomainDisA/LigA, helix-hairpin-helix motifInterproscan

 PANTHER
PANTHER termDescriptionSource
PTHR23389
all species →
CHROMOSOME TRANSMISSION FIDELITY FACTOR 18Interproscan

 Gene Ontology
GO termCategoryDescriptionSource
GO:0003911
all species →
Molecular FunctionDNA ligase (NAD+) activityInterproscan
GO:0006260
all species →
Biological ProcessDNA replicationInterproscan
GO:0006281
all species →
Biological ProcessDNA repairInterproscan
GO:0005829
all species →
Cellular ComponentcytosolInterproscan
GO:0006288
all species →
Biological Processbase-excision repair, DNA ligationInterproscan

Search by domain instead of by gene. Any accession above (InterPro, Pfam, PANTHER, GO, KEGG) can be used as a query on the Functional Domain Search page, which searches all 148 annotated genomes at once.
 KEGG pathway
KOEnzymeEnzyme IDPathwayMap IDSource
K01972E6.5.1.2, ligA, ligB; DNA ligase (NAD+)EC:6.5.1.2
DNA repair and recombination proteinsko03400deepkoala

Searching by KO or pathway ID across all species is available on the KEGG Pathway page.

Expression pattern (RNA-seq)

Transcript abundance of HSymV2.0_g02.02262_t1 across 44 RNA-seq samples of Hydractinia symbiolongicarpus. This gene has no row in the species' RNA-seq expression matrix, so every value below is shown as zero — the matrix simply does not cover this transcript. Values are TPM (transcripts per million) from the StringTie quantification; one bar is one sample, grouped and coloured by condition, sorted by expression within each group.

44Samples
0TPM > 0
3Conditions
0.0Max TPM
0.0Mean TPM

By condition

ConditionSamplesTPM > 0 Mean TPMMax TPMMean, relative to max
Whole embryo 36 0 0.00 0.00
Whole embryo · Triptolide 20 uM 4 0 0.00 0.00
Whole embryo · DMSO 0.5% 4 0 0.00 0.00

Per sample · hover a bar for the full sample record

Show the sample table (44 samples)
SRA runConditionTissueDevelopmental stage TreatmentStudyTPM
SRR24482133 Whole embryo Whole embryo 6 hpf not recorded SRP436676 0.00
SRR24482134 Whole embryo Whole embryo 5 hpf not recorded SRP436676 0.00
SRR24482135 Whole embryo Whole embryo 4 hpf not recorded SRP436676 0.00
SRR24482136 Whole embryo Whole embryo 4 hpf not recorded SRP436676 0.00
SRR24482137 Whole embryo Whole embryo 3 hpf not recorded SRP436676 0.00
SRR24482138 Whole embryo Whole embryo 3 hpf not recorded SRP436676 0.00
SRR24482139 Whole embryo Whole embryo 2 hpf not recorded SRP436676 0.00
SRR24482140 Whole embryo Whole embryo 2 hpf not recorded SRP436676 0.00
SRR24482141 Whole embryo Whole embryo 1 hpf not recorded SRP436676 0.00
SRR24482142 Whole embryo Whole embryo 30 mpf not recorded SRP436676 0.00
SRR24482143 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 0.00
SRR24482144 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 0.00
SRR24482145 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 0.00
SRR24482153 Whole embryo Whole embryo 7 hpf not recorded SRP436676 0.00
SRR24482155 Whole embryo Whole embryo 72 hpf not recorded SRP436676 0.00
SRR24482156 Whole embryo Whole embryo 72 hpf not recorded SRP436676 0.00
SRR24482157 Whole embryo Whole embryo 48 hpf not recorded SRP436676 0.00
SRR24482158 Whole embryo Whole embryo 48 hpf not recorded SRP436676 0.00
SRR24482159 Whole embryo Whole embryo 24 hpf not recorded SRP436676 0.00
SRR24482160 Whole embryo Whole embryo 24 hpf not recorded SRP436676 0.00
SRR24482161 Whole embryo Whole embryo 7 hpf not recorded SRP436676 0.00
SRR24482162 Whole embryo Whole embryo 7 hpf not recorded SRP436676 0.00
SRR24482163 Whole embryo Whole embryo 6 hpf not recorded SRP436676 0.00
SRR24482165 Whole embryo Whole embryo 4 hpf not recorded SRP436676 0.00
SRR24482166 Whole embryo Whole embryo 4 hpf not recorded SRP436676 0.00
SRR24482167 Whole embryo Whole embryo 3 hpf not recorded SRP436676 0.00
SRR24482168 Whole embryo Whole embryo 3 hpf not recorded SRP436676 0.00
SRR24482169 Whole embryo Whole embryo 2 hpf not recorded SRP436676 0.00
SRR24482170 Whole embryo Whole embryo 2 hpf not recorded SRP436676 0.00
SRR24482171 Whole embryo Whole embryo 1 hpf not recorded SRP436676 0.00
SRR24482172 Whole embryo Whole embryo 30 mpf not recorded SRP436676 0.00
SRR24482173 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 0.00
SRR24482174 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 0.00
SRR24482175 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 0.00
SRR24482176 Whole embryo Whole embryo 1 hpf not recorded SRP436676 0.00
SRR24482177 Whole embryo Whole embryo 1 hpf not recorded SRP436676 0.00
SRR24482146 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 0.00
SRR24482147 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 0.00
SRR24482149 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 0.00
SRR24482150 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 0.00
SRR24482148 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 0.00
SRR24482151 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 0.00
SRR24482152 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 0.00
SRR24482154 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 0.00

Source: CnidoSite RNA-seq expression matrices (HSYMB_TPM, StringTie quantification over 44 runs), joined to SRA sample metadata. Samples whose tissue/treatment is not recorded in the source metadata are grouped by the descriptor carried in the expression matrix itself.

Co-expression network

Genes whose expression across the transcriptome samples of Hydractinia symbiolongicarpus tracks this one. Counts are over the whole network; the network view itself draws at most 100 partners per query gene (CNIDO_NET_TOP_K), so a hub gene can show fewer edges than the number below.

DirectionPartnersMost correlated partnerPCC
Positively correlated0not in this network-
Negatively correlated0not in this network-

This gene has no edge at all in the Hydractinia symbiolongicarpus network, in either direction — it is not one of the genes the network was built from (the network covers genes with enough expression variation across the transcriptome samples). The counts above are a property of the network, not a failed lookup.

Open this gene in Network Analysis → The network opens with the gene already entered and both directions selected. There you can add up to 9 more genes, switch between the positive and negative network, and export the edge list.

Single-cell expression

Whether this gene can be visualised in the single-cell atlases of Hydractinia symbiolongicarpus, and in which cell types it is a marker. Expression is stored per cell, so the violin plot and the cell-type means are computed in the viewer — open a dataset to see them.

No single-cell dataset has been published for this species. The atlases that do exist are listed in the Cell Atlas.

Epigenetic marks

Chromatin and DNA-methylation data covering this gene. Peak calls are listed per sample with the genomic region the peak falls in; DNA methylation is listed as sample availability only, because those tables are queried by the DNA Methylation page itself.

No epigenomic data has been deposited for this species. The assays that exist across the site are described in the Epigenomic Data module.

What you can do with this gene

Every tool below opens with this gene already entered, so you land on the analysis rather than on an empty form. Links open in a new tab.

AnalysisWhat it doesStatus
Primer designDesign PCR / qPCR primers on this gene’s sequence with the published primer3 settings. Opens with the primers already computed.template foundopen →
BLASTSearch this gene’s sequence against the CnidoSite BLAST databases. The query is filled in and the species’ database is preselected — press Run to start.template foundopen →
Expression heatmapDraw this gene’s expression as a heatmap across all RNA-seq samples, and compare it with the genes you add next to it.no expression matrix
Gene family / orthogroupLook this gene up in the single-copy orthogroups built from the high-quality cnidarian genomes, and see which other species carry an orthologue.open →
Gene set analysisStart a gene-set enrichment analysis with this gene as the seed list. Add more genes on that page before running it.open →
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