Detailed information of HSymV2.0_g02.02355_t1 in Hydractinia symbiolongicarpus

Genomic Location: HiC_scaffold_2:703588...705459
NR annotation: MBQ4874597.1, RNA polymerase sigma factor RpoD [Rickettsiaceae bacterium H1]
Species Hydractinia symbiolongicarpus · all data for this species · gene families


 Gene Structure
More details in JBrowse  Sequence
CDS
Transcript
Protein
 UniProt (Swiss-Prot top hit)
UniProt accessionDescription
P33452RNA polymerase sigma factor RpoD OS=Agrobacterium fabrum (strain C58 / ATCC 33970) OX=176299 GN=rpoD PE=3 SV=2
Q59753RNA polymerase sigma factor RpoD OS=Rhizobium meliloti (strain 1021) OX=266834 GN=rpoD PE=3 SV=1
Q2K619RNA polymerase sigma factor RpoD OS=Rhizobium etli (strain ATCC 51251 / DSM 11541 / JCM 21823 / NBRC 15573 / CFN 42) OX=347834 GN=rpoD PE=1 SV=1
 Gene family
Family typeMembership / link
Orthogroup (gene family)OG0013255 (this species only) · gene tree & orthology

 Pfam domain
Pfam accessionPfam nameDescriptionTypeSource
PF00140
all species →
Sigma70_r1_2Sigma-70 factor, region 1.2FamilyInterproscan
PF03979
all species →
Sigma70_r1_1Sigma-70 factor, region 1.1FamilyInterproscan
PF04542
all species →
Sigma70_r2Sigma-70 region 2 DomainInterproscan
PF04546
all species →
Sigma70_nerSigma-70, non-essential regionDomainInterproscan
PF04545
all species →
Sigma70_r4Sigma-70, region 4DomainInterproscan
PF04539
all species →
Sigma70_r3Sigma-70 region 3FamilyInterproscan

 InterPro
InterPro termTypeDescriptionSource
IPR009042
all species →
DomainRNA polymerase sigma-70 region 1.2Interproscan
IPR050239
all species →
FamilySigma-70 factor family, RNA polymerase initiation factorsInterproscan
IPR013324
all species →
Homologous_superfamilyRNA polymerase sigma factor, region 3/4-likeInterproscan
IPR007127
all species →
DomainRNA polymerase sigma factor 70, region 1.1Interproscan
IPR007627
all species →
DomainRNA polymerase sigma-70 region 2Interproscan
IPR013325
all species →
Homologous_superfamilyRNA polymerase sigma factor, region 2Interproscan
IPR000943
all species →
DomainRNA polymerase sigma-70Interproscan
IPR042189
all species →
Homologous_superfamilySigma-70 factor, region 1.1 superfamilyInterproscan
IPR036388
all species →
Homologous_superfamilyWinged helix-like DNA-binding domain superfamilyInterproscan
IPR007631
all species →
DomainRNA polymerase sigma factor 70, non-essential domainInterproscan
IPR014284
all species →
DomainRNA polymerase sigma-70 like domainInterproscan
IPR028630
all species →
FamilyRNA polymerase sigma factor RpoDInterproscan
IPR007630
all species →
DomainRNA polymerase sigma-70 region 4Interproscan
IPR007624
all species →
DomainRNA polymerase sigma-70 region 3Interproscan

 PANTHER
PANTHER termDescriptionSource
PTHR30603
all species →
RNA POLYMERASE SIGMA FACTOR RPOInterproscan

 Gene Ontology
GO termCategoryDescriptionSource
GO:0003677
all species →
Molecular FunctionDNA bindingInterproscan
GO:0003700
all species →
Molecular FunctionDNA-binding transcription factor activityInterproscan
GO:0006352
all species →
Biological ProcessDNA-templated transcription initiationInterproscan
GO:0006355
all species →
Biological Processregulation of DNA-templated transcriptionInterproscan
GO:0016987
all species →
Molecular Functionsigma factor activityInterproscan

Search by domain instead of by gene. Any accession above (InterPro, Pfam, PANTHER, GO, KEGG) can be used as a query on the Functional Domain Search page, which searches all 148 annotated genomes at once.
 KEGG pathway
KOEnzymeEnzyme IDPathwayMap IDSource
K03086rpoD; RNA polymerase primary sigma factor-Transcription machineryko03021deepkoala

Searching by KO or pathway ID across all species is available on the KEGG Pathway page.

Expression pattern (RNA-seq)

Transcript abundance of HSymV2.0_g02.02355_t1 across 44 RNA-seq samples of Hydractinia symbiolongicarpus. This gene has no row in the species' RNA-seq expression matrix, so every value below is shown as zero — the matrix simply does not cover this transcript. Values are TPM (transcripts per million) from the StringTie quantification; one bar is one sample, grouped and coloured by condition, sorted by expression within each group.

44Samples
0TPM > 0
3Conditions
0.0Max TPM
0.0Mean TPM

By condition

ConditionSamplesTPM > 0 Mean TPMMax TPMMean, relative to max
Whole embryo 36 0 0.00 0.00
Whole embryo · Triptolide 20 uM 4 0 0.00 0.00
Whole embryo · DMSO 0.5% 4 0 0.00 0.00

Per sample · hover a bar for the full sample record

Show the sample table (44 samples)
SRA runConditionTissueDevelopmental stage TreatmentStudyTPM
SRR24482133 Whole embryo Whole embryo 6 hpf not recorded SRP436676 0.00
SRR24482134 Whole embryo Whole embryo 5 hpf not recorded SRP436676 0.00
SRR24482135 Whole embryo Whole embryo 4 hpf not recorded SRP436676 0.00
SRR24482136 Whole embryo Whole embryo 4 hpf not recorded SRP436676 0.00
SRR24482137 Whole embryo Whole embryo 3 hpf not recorded SRP436676 0.00
SRR24482138 Whole embryo Whole embryo 3 hpf not recorded SRP436676 0.00
SRR24482139 Whole embryo Whole embryo 2 hpf not recorded SRP436676 0.00
SRR24482140 Whole embryo Whole embryo 2 hpf not recorded SRP436676 0.00
SRR24482141 Whole embryo Whole embryo 1 hpf not recorded SRP436676 0.00
SRR24482142 Whole embryo Whole embryo 30 mpf not recorded SRP436676 0.00
SRR24482143 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 0.00
SRR24482144 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 0.00
SRR24482145 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 0.00
SRR24482153 Whole embryo Whole embryo 7 hpf not recorded SRP436676 0.00
SRR24482155 Whole embryo Whole embryo 72 hpf not recorded SRP436676 0.00
SRR24482156 Whole embryo Whole embryo 72 hpf not recorded SRP436676 0.00
SRR24482157 Whole embryo Whole embryo 48 hpf not recorded SRP436676 0.00
SRR24482158 Whole embryo Whole embryo 48 hpf not recorded SRP436676 0.00
SRR24482159 Whole embryo Whole embryo 24 hpf not recorded SRP436676 0.00
SRR24482160 Whole embryo Whole embryo 24 hpf not recorded SRP436676 0.00
SRR24482161 Whole embryo Whole embryo 7 hpf not recorded SRP436676 0.00
SRR24482162 Whole embryo Whole embryo 7 hpf not recorded SRP436676 0.00
SRR24482163 Whole embryo Whole embryo 6 hpf not recorded SRP436676 0.00
SRR24482165 Whole embryo Whole embryo 4 hpf not recorded SRP436676 0.00
SRR24482166 Whole embryo Whole embryo 4 hpf not recorded SRP436676 0.00
SRR24482167 Whole embryo Whole embryo 3 hpf not recorded SRP436676 0.00
SRR24482168 Whole embryo Whole embryo 3 hpf not recorded SRP436676 0.00
SRR24482169 Whole embryo Whole embryo 2 hpf not recorded SRP436676 0.00
SRR24482170 Whole embryo Whole embryo 2 hpf not recorded SRP436676 0.00
SRR24482171 Whole embryo Whole embryo 1 hpf not recorded SRP436676 0.00
SRR24482172 Whole embryo Whole embryo 30 mpf not recorded SRP436676 0.00
SRR24482173 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 0.00
SRR24482174 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 0.00
SRR24482175 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 0.00
SRR24482176 Whole embryo Whole embryo 1 hpf not recorded SRP436676 0.00
SRR24482177 Whole embryo Whole embryo 1 hpf not recorded SRP436676 0.00
SRR24482146 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 0.00
SRR24482147 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 0.00
SRR24482149 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 0.00
SRR24482150 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 0.00
SRR24482148 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 0.00
SRR24482151 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 0.00
SRR24482152 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 0.00
SRR24482154 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 0.00

Source: CnidoSite RNA-seq expression matrices (HSYMB_TPM, StringTie quantification over 44 runs), joined to SRA sample metadata. Samples whose tissue/treatment is not recorded in the source metadata are grouped by the descriptor carried in the expression matrix itself.

Co-expression network

Genes whose expression across the transcriptome samples of Hydractinia symbiolongicarpus tracks this one. Counts are over the whole network; the network view itself draws at most 100 partners per query gene (CNIDO_NET_TOP_K), so a hub gene can show fewer edges than the number below.

DirectionPartnersMost correlated partnerPCC
Positively correlated0not in this network-
Negatively correlated0not in this network-

This gene has no edge at all in the Hydractinia symbiolongicarpus network, in either direction — it is not one of the genes the network was built from (the network covers genes with enough expression variation across the transcriptome samples). The counts above are a property of the network, not a failed lookup.

Open this gene in Network Analysis → The network opens with the gene already entered and both directions selected. There you can add up to 9 more genes, switch between the positive and negative network, and export the edge list.

Single-cell expression

Whether this gene can be visualised in the single-cell atlases of Hydractinia symbiolongicarpus, and in which cell types it is a marker. Expression is stored per cell, so the violin plot and the cell-type means are computed in the viewer — open a dataset to see them.

No single-cell dataset has been published for this species. The atlases that do exist are listed in the Cell Atlas.

Epigenetic marks

Chromatin and DNA-methylation data covering this gene. Peak calls are listed per sample with the genomic region the peak falls in; DNA methylation is listed as sample availability only, because those tables are queried by the DNA Methylation page itself.

No epigenomic data has been deposited for this species. The assays that exist across the site are described in the Epigenomic Data module.

What you can do with this gene

Every tool below opens with this gene already entered, so you land on the analysis rather than on an empty form. Links open in a new tab.

AnalysisWhat it doesStatus
Primer designDesign PCR / qPCR primers on this gene’s sequence with the published primer3 settings. Opens with the primers already computed.template foundopen →
BLASTSearch this gene’s sequence against the CnidoSite BLAST databases. The query is filled in and the species’ database is preselected — press Run to start.template foundopen →
Expression heatmapDraw this gene’s expression as a heatmap across all RNA-seq samples, and compare it with the genes you add next to it.no expression matrix
Gene family / orthogroupLook this gene up in the single-copy orthogroups built from the high-quality cnidarian genomes, and see which other species carry an orthologue.open →
Gene set analysisStart a gene-set enrichment analysis with this gene as the seed list. Add more genes on that page before running it.open →
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