Gene detail

Detailed information of HSymV2.0_g03.05022_t1 in Hydractinia symbiolongicarpus

Genomic Location: HiC_scaffold_3:16118791...16126937
NR annotation: XP_047130180.1, cullin-4A [Hydra vulgaris]
Species Hydractinia symbiolongicarpus · all data for this species · gene families

Gene Structure

More details in JBrowse

Sequence

CDS

Transcript

Protein

UniProt (Swiss-Prot top hit)

UniProt accession	Description
Q13619	Cullin-4A OS=Homo sapiens OX=9606 GN=CUL4A PE=1 SV=3
Q3TCH7	Cullin-4A OS=Mus musculus OX=10090 GN=Cul4a PE=1 SV=1
A2A432	Cullin-4B OS=Mus musculus OX=10090 GN=Cul4b PE=1 SV=1

Gene family

Family type	Membership / link
Orthogroup (gene family)	OG0000557 (this species only) · gene tree & orthology

Pfam domain

Pfam accession	Pfam name	Description	Type	Source
PF00888 all species →	Cullin	Cullin family	Repeat	Interproscan
PF10557 all species →	Cullin_Nedd8	Cullin protein neddylation domain	Domain	Interproscan

InterPro

InterPro term	Type	Description	Source
IPR019559 all species →	Domain	Cullin protein, neddylation domain	Interproscan
IPR045093 all species →	Family	Cullin	Interproscan
IPR016157 all species →	Conserved_site	Cullin, conserved site	Interproscan
IPR001373 all species →	Domain	Cullin, N-terminal	Interproscan
IPR016159 all species →	Homologous_superfamily	Cullin repeat-like-containing domain superfamily	Interproscan
IPR036388 all species →	Homologous_superfamily	Winged helix-like DNA-binding domain superfamily	Interproscan
IPR036390 all species →	Homologous_superfamily	Winged helix DNA-binding domain superfamily	Interproscan
IPR036317 all species →	Homologous_superfamily	Cullin homology domain superfamily	Interproscan
IPR016158 all species →	Domain	Cullin homology domain	Interproscan

PANTHER

PANTHER term	Description	Source
PTHR11932 all species →	CULLIN	Interproscan

Gene Ontology

GO term	Category	Description	Source
GO:0006974 all species →	Biological Process	DNA damage response	Interproscan
GO:0016567 all species →	Biological Process	protein ubiquitination	Interproscan
GO:0031461 all species →	Cellular Component	cullin-RING ubiquitin ligase complex	Interproscan
GO:0031625 all species →	Molecular Function	ubiquitin protein ligase binding	Interproscan
GO:0080008 all species →	Cellular Component	Cul4-RING E3 ubiquitin ligase complex	Interproscan
GO:0006511 all species →	Biological Process	ubiquitin-dependent protein catabolic process	Interproscan

Search by domain instead of by gene. Any accession above (InterPro, Pfam, PANTHER, GO, KEGG) can be used as a query on the Functional Domain Search page, which searches all 148 annotated genomes at once.

KEGG pathway

KO	Enzyme	Enzyme ID	Pathway	Map ID	Source
K10609	CUL4; cullin 4	-	Exosome	ko04147	deepkoala

Searching by KO or pathway ID across all species is available on the KEGG Pathway page.

Expression pattern (RNA-seq)

Transcript abundance of HSymV2.0_g03.05022_t1 across 44 RNA-seq samples of Hydractinia symbiolongicarpus. Values are TPM (transcripts per million) from the StringTie quantification; one bar is one sample, grouped and coloured by condition, sorted by expression within each group.

44Samples

44TPM > 0

3Conditions

144.5Max TPM

91.0Mean TPM

By condition

Condition	Samples	TPM > 0	Mean TPM	Max TPM
Whole embryo	36	36	96.77	144.54
Whole embryo · Triptolide 20 uM	4	4	82.75	119.56
Whole embryo · DMSO 0.5%	4	4	47.58	62.61

Per sample · hover a bar for the full sample record

Show the sample table (44 samples)

SRA run	Condition	Tissue	Developmental stage	Treatment	Study	TPM
SRR24482143	Whole embryo	Whole embryo	Unfertilized egg	not recorded	SRP436676	144.54
SRR24482137	Whole embryo	Whole embryo	3 hpf	not recorded	SRP436676	144.13
SRR24482139	Whole embryo	Whole embryo	2 hpf	not recorded	SRP436676	142.17
SRR24482177	Whole embryo	Whole embryo	1 hpf	not recorded	SRP436676	141.74
SRR24482135	Whole embryo	Whole embryo	4 hpf	not recorded	SRP436676	140.17
SRR24482144	Whole embryo	Whole embryo	Unfertilized egg	not recorded	SRP436676	139.23
SRR24482138	Whole embryo	Whole embryo	3 hpf	not recorded	SRP436676	127.19
SRR24482141	Whole embryo	Whole embryo	1 hpf	not recorded	SRP436676	121.68
SRR24482142	Whole embryo	Whole embryo	30 mpf	not recorded	SRP436676	120.77
SRR24482145	Whole embryo	Whole embryo	Unfertilized egg	not recorded	SRP436676	120.14
SRR24482134	Whole embryo	Whole embryo	5 hpf	not recorded	SRP436676	119.25
SRR24482140	Whole embryo	Whole embryo	2 hpf	not recorded	SRP436676	116.32
SRR24482174	Whole embryo	Whole embryo	Unfertilized egg	not recorded	SRP436676	115.52
SRR24482165	Whole embryo	Whole embryo	4 hpf	not recorded	SRP436676	115.40
SRR24482167	Whole embryo	Whole embryo	3 hpf	not recorded	SRP436676	111.12
SRR24482136	Whole embryo	Whole embryo	4 hpf	not recorded	SRP436676	109.54
SRR24482169	Whole embryo	Whole embryo	2 hpf	not recorded	SRP436676	104.95
SRR24482173	Whole embryo	Whole embryo	Unfertilized egg	not recorded	SRP436676	104.04
SRR24482176	Whole embryo	Whole embryo	1 hpf	not recorded	SRP436676	96.48
SRR24482166	Whole embryo	Whole embryo	4 hpf	not recorded	SRP436676	91.42
SRR24482172	Whole embryo	Whole embryo	30 mpf	not recorded	SRP436676	89.81
SRR24482133	Whole embryo	Whole embryo	6 hpf	not recorded	SRP436676	83.91
SRR24482168	Whole embryo	Whole embryo	3 hpf	not recorded	SRP436676	81.29
SRR24482171	Whole embryo	Whole embryo	1 hpf	not recorded	SRP436676	80.97
SRR24482170	Whole embryo	Whole embryo	2 hpf	not recorded	SRP436676	78.94
SRR24482157	Whole embryo	Whole embryo	48 hpf	not recorded	SRP436676	77.11
SRR24482160	Whole embryo	Whole embryo	24 hpf	not recorded	SRP436676	76.77
SRR24482175	Whole embryo	Whole embryo	Unfertilized egg	not recorded	SRP436676	70.54
SRR24482155	Whole embryo	Whole embryo	72 hpf	not recorded	SRP436676	67.75
SRR24482159	Whole embryo	Whole embryo	24 hpf	not recorded	SRP436676	63.25
SRR24482158	Whole embryo	Whole embryo	48 hpf	not recorded	SRP436676	56.84
SRR24482153	Whole embryo	Whole embryo	7 hpf	not recorded	SRP436676	51.57
SRR24482163	Whole embryo	Whole embryo	6 hpf	not recorded	SRP436676	49.35
SRR24482162	Whole embryo	Whole embryo	7 hpf	not recorded	SRP436676	48.20
SRR24482156	Whole embryo	Whole embryo	72 hpf	not recorded	SRP436676	46.80
SRR24482161	Whole embryo	Whole embryo	7 hpf	not recorded	SRP436676	34.68
SRR24482150	Whole embryo · Triptolide 20 uM	Whole embryo	7 hpf	Triptolide 20 uM	SRP436676	119.56
SRR24482149	Whole embryo · Triptolide 20 uM	Whole embryo	7 hpf	Triptolide 20 uM	SRP436676	111.77
SRR24482146	Whole embryo · Triptolide 20 uM	Whole embryo	7 hpf	Triptolide 20 uM	SRP436676	49.99
SRR24482147	Whole embryo · Triptolide 20 uM	Whole embryo	7 hpf	Triptolide 20 uM	SRP436676	49.69
SRR24482152	Whole embryo · DMSO 0.5%	Whole embryo	7 hpf	DMSO 0.5%	SRP436676	62.61
SRR24482151	Whole embryo · DMSO 0.5%	Whole embryo	7 hpf	DMSO 0.5%	SRP436676	57.37
SRR24482154	Whole embryo · DMSO 0.5%	Whole embryo	7 hpf	DMSO 0.5%	SRP436676	36.18
SRR24482148	Whole embryo · DMSO 0.5%	Whole embryo	7 hpf	DMSO 0.5%	SRP436676	34.15

Source: CnidoSite RNA-seq expression matrices (HSYMB_TPM, StringTie quantification over 44 runs), joined to SRA sample metadata. Samples whose tissue/treatment is not recorded in the source metadata are grouped by the descriptor carried in the expression matrix itself.

Co-expression network

Genes whose expression across the transcriptome samples of Hydractinia symbiolongicarpus tracks this one. Counts are over the whole network; the network view itself draws at most 100 partners per query gene (CNIDO_NET_TOP_K), so a hub gene can show fewer edges than the number below.

Direction	Partners	Most correlated partner	PCC
Positively correlated	16	HSymV2.0_g09.16783_t1	0.951548964777542
Negatively correlated	21	HSymV2.0_g07.12210_t1	-0.907359043520565

Open this gene in Network Analysis → The network opens with the gene already entered and both directions selected. There you can add up to 9 more genes, switch between the positive and negative network, and export the edge list.

Single-cell expression

Whether this gene can be visualised in the single-cell atlases of Hydractinia symbiolongicarpus, and in which cell types it is a marker. Expression is stored per cell, so the violin plot and the cell-type means are computed in the viewer — open a dataset to see them.

No single-cell dataset has been published for this species. The atlases that do exist are listed in the Cell Atlas.

Epigenetic marks

Chromatin and DNA-methylation data covering this gene. Peak calls are listed per sample with the genomic region the peak falls in; DNA methylation is listed as sample availability only, because those tables are queried by the DNA Methylation page itself.

No epigenomic data has been deposited for this species. The assays that exist across the site are described in the Epigenomic Data module.

What you can do with this gene

Every tool below opens with this gene already entered, so you land on the analysis rather than on an empty form. Links open in a new tab.

Analysis	What it does	Status
Primer design	Design PCR / qPCR primers on this gene’s sequence with the published primer3 settings. Opens with the primers already computed.	template found	open →
BLAST	Search this gene’s sequence against the CnidoSite BLAST databases. The query is filled in and the species’ database is preselected — press Run to start.	template found	open →
Expression heatmap	Draw this gene’s expression as a heatmap across all RNA-seq samples, and compare it with the genes you add next to it.	ready	open →
Gene family / orthogroup	Look this gene up in the single-copy orthogroups built from the high-quality cnidarian genomes, and see which other species carry an orthologue.		open →
Gene set analysis	Start a gene-set enrichment analysis with this gene as the seed list. Add more genes on that page before running it.		open →