Gene detail

Detailed information of HSymV2.0_g03.05549_t1 in Hydractinia symbiolongicarpus

Genomic Location: HiC_scaffold_3:24503388...24514233
NR annotation: XP_002158042.2, 26S proteasome regulatory subunit 6B [Hydra vulgaris]
Species Hydractinia symbiolongicarpus · all data for this species · gene families

Gene Structure

More details in JBrowse

Sequence

CDS

Transcript

Protein

UniProt (Swiss-Prot top hit)

UniProt accession	Description
Q3T030	26S proteasome regulatory subunit 6B OS=Bos taurus OX=9913 GN=PSMC4 PE=2 SV=1
P43686	26S proteasome regulatory subunit 6B OS=Homo sapiens OX=9606 GN=PSMC4 PE=1 SV=2
Q4R7L3	26S proteasome regulatory subunit 6B OS=Macaca fascicularis OX=9541 GN=PSMC4 PE=2 SV=1

Gene family

Family type	Membership / link
Orthogroup (gene family)	OG0000576 (this species only) · gene tree & orthology

Pfam domain

Pfam accession	Pfam name	Description	Type	Source
PF00004 all species →	AAA	ATPase family associated with various cellular activities (AAA)	Domain	Interproscan
PF16450 all species →	Prot_ATP_ID_OB_C	Proteasomal ATPase OB C-terminal domain	Domain	Interproscan
PF17862 all species →	AAA_lid_3	AAA+ lid domain	Domain	Interproscan

InterPro

InterPro term	Type	Description	Source
IPR003959 all species →	Domain	ATPase, AAA-type, core	Interproscan
IPR003960 all species →	Conserved_site	ATPase, AAA-type, conserved site	Interproscan
IPR032501 all species →	Domain	Proteasomal ATPase, second OB domain	Interproscan
IPR041569 all species →	Domain	AAA ATPase, AAA+ lid domain	Interproscan
IPR003593 all species →	Domain	AAA+ ATPase domain	Interproscan
IPR027417 all species →	Homologous_superfamily	P-loop containing nucleoside triphosphate hydrolase	Interproscan
IPR012340 all species →	Homologous_superfamily	Nucleic acid-binding, OB-fold	Interproscan
IPR050221 all species →	Family	26S Proteasome Regulatory ATPase	Interproscan

PANTHER

PANTHER term	Description	Source
PTHR23073 all species →	26S PROTEASOME REGULATORY SUBUNIT	Interproscan

Gene Ontology

GO term	Category	Description	Source
GO:0005524 all species →	Molecular Function	ATP binding	Interproscan
GO:0016887 all species →	Molecular Function	ATP hydrolysis activity	Interproscan
GO:0008540 all species →	Cellular Component	proteasome regulatory particle, base subcomplex	Interproscan
GO:0036402 all species →	Molecular Function	proteasome-activating activity	Interproscan
GO:0043161 all species →	Biological Process	proteasome-mediated ubiquitin-dependent protein catabolic process	Interproscan

Search by domain instead of by gene. Any accession above (InterPro, Pfam, PANTHER, GO, KEGG) can be used as a query on the Functional Domain Search page, which searches all 148 annotated genomes at once.

KEGG pathway

KO	Enzyme	Enzyme ID	Pathway	Map ID	Source
K03063	PSMC4, RPT3; 26S proteasome regulatory subunit T3	-	Proteasome	ko03051	deepkoala

Searching by KO or pathway ID across all species is available on the KEGG Pathway page.

Expression pattern (RNA-seq)

Transcript abundance of HSymV2.0_g03.05549_t1 across 44 RNA-seq samples of Hydractinia symbiolongicarpus. Values are TPM (transcripts per million) from the StringTie quantification; one bar is one sample, grouped and coloured by condition, sorted by expression within each group.

44Samples

44TPM > 0

3Conditions

195.5Max TPM

138.5Mean TPM

By condition

Condition	Samples	TPM > 0	Mean TPM	Max TPM
Whole embryo	36	36	137.78	195.51
Whole embryo · Triptolide 20 uM	4	4	136.91	139.63
Whole embryo · DMSO 0.5%	4	4	146.12	159.49

Per sample · hover a bar for the full sample record

Show the sample table (44 samples)

SRA run	Condition	Tissue	Developmental stage	Treatment	Study	TPM
SRR24482176	Whole embryo	Whole embryo	1 hpf	not recorded	SRP436676	195.51
SRR24482169	Whole embryo	Whole embryo	2 hpf	not recorded	SRP436676	181.61
SRR24482173	Whole embryo	Whole embryo	Unfertilized egg	not recorded	SRP436676	174.74
SRR24482166	Whole embryo	Whole embryo	4 hpf	not recorded	SRP436676	174.52
SRR24482170	Whole embryo	Whole embryo	2 hpf	not recorded	SRP436676	169.38
SRR24482160	Whole embryo	Whole embryo	24 hpf	not recorded	SRP436676	167.47
SRR24482167	Whole embryo	Whole embryo	3 hpf	not recorded	SRP436676	166.29
SRR24482174	Whole embryo	Whole embryo	Unfertilized egg	not recorded	SRP436676	163.67
SRR24482161	Whole embryo	Whole embryo	7 hpf	not recorded	SRP436676	162.94
SRR24482171	Whole embryo	Whole embryo	1 hpf	not recorded	SRP436676	161.28
SRR24482165	Whole embryo	Whole embryo	4 hpf	not recorded	SRP436676	155.60
SRR24482159	Whole embryo	Whole embryo	24 hpf	not recorded	SRP436676	154.31
SRR24482168	Whole embryo	Whole embryo	3 hpf	not recorded	SRP436676	152.18
SRR24482162	Whole embryo	Whole embryo	7 hpf	not recorded	SRP436676	148.44
SRR24482175	Whole embryo	Whole embryo	Unfertilized egg	not recorded	SRP436676	147.19
SRR24482158	Whole embryo	Whole embryo	48 hpf	not recorded	SRP436676	142.79
SRR24482163	Whole embryo	Whole embryo	6 hpf	not recorded	SRP436676	139.83
SRR24482137	Whole embryo	Whole embryo	3 hpf	not recorded	SRP436676	138.56
SRR24482143	Whole embryo	Whole embryo	Unfertilized egg	not recorded	SRP436676	136.60
SRR24482133	Whole embryo	Whole embryo	6 hpf	not recorded	SRP436676	132.46
SRR24482135	Whole embryo	Whole embryo	4 hpf	not recorded	SRP436676	131.93
SRR24482157	Whole embryo	Whole embryo	48 hpf	not recorded	SRP436676	129.56
SRR24482139	Whole embryo	Whole embryo	2 hpf	not recorded	SRP436676	128.90
SRR24482144	Whole embryo	Whole embryo	Unfertilized egg	not recorded	SRP436676	127.34
SRR24482172	Whole embryo	Whole embryo	30 mpf	not recorded	SRP436676	125.88
SRR24482156	Whole embryo	Whole embryo	72 hpf	not recorded	SRP436676	125.63
SRR24482177	Whole embryo	Whole embryo	1 hpf	not recorded	SRP436676	123.54
SRR24482140	Whole embryo	Whole embryo	2 hpf	not recorded	SRP436676	112.65
SRR24482155	Whole embryo	Whole embryo	72 hpf	not recorded	SRP436676	111.83
SRR24482153	Whole embryo	Whole embryo	7 hpf	not recorded	SRP436676	105.93
SRR24482145	Whole embryo	Whole embryo	Unfertilized egg	not recorded	SRP436676	99.84
SRR24482134	Whole embryo	Whole embryo	5 hpf	not recorded	SRP436676	98.75
SRR24482142	Whole embryo	Whole embryo	30 mpf	not recorded	SRP436676	96.89
SRR24482141	Whole embryo	Whole embryo	1 hpf	not recorded	SRP436676	96.59
SRR24482138	Whole embryo	Whole embryo	3 hpf	not recorded	SRP436676	91.12
SRR24482136	Whole embryo	Whole embryo	4 hpf	not recorded	SRP436676	88.34
SRR24482147	Whole embryo · Triptolide 20 uM	Whole embryo	7 hpf	Triptolide 20 uM	SRP436676	139.63
SRR24482146	Whole embryo · Triptolide 20 uM	Whole embryo	7 hpf	Triptolide 20 uM	SRP436676	138.93
SRR24482149	Whole embryo · Triptolide 20 uM	Whole embryo	7 hpf	Triptolide 20 uM	SRP436676	138.07
SRR24482150	Whole embryo · Triptolide 20 uM	Whole embryo	7 hpf	Triptolide 20 uM	SRP436676	131.03
SRR24482154	Whole embryo · DMSO 0.5%	Whole embryo	7 hpf	DMSO 0.5%	SRP436676	159.49
SRR24482148	Whole embryo · DMSO 0.5%	Whole embryo	7 hpf	DMSO 0.5%	SRP436676	152.63
SRR24482152	Whole embryo · DMSO 0.5%	Whole embryo	7 hpf	DMSO 0.5%	SRP436676	140.41
SRR24482151	Whole embryo · DMSO 0.5%	Whole embryo	7 hpf	DMSO 0.5%	SRP436676	131.95

Source: CnidoSite RNA-seq expression matrices (HSYMB_TPM, StringTie quantification over 44 runs), joined to SRA sample metadata. Samples whose tissue/treatment is not recorded in the source metadata are grouped by the descriptor carried in the expression matrix itself.

Co-expression network

Genes whose expression across the transcriptome samples of Hydractinia symbiolongicarpus tracks this one. Counts are over the whole network; the network view itself draws at most 100 partners per query gene (CNIDO_NET_TOP_K), so a hub gene can show fewer edges than the number below.

Direction	Partners	Most correlated partner	PCC
Positively correlated	12	HSymV2.0_g15.24939_t1	0.82200842119959
Negatively correlated	91	HSymV2.0_g09.17102_t1	-0.820734736559416

Open this gene in Network Analysis → The network opens with the gene already entered and both directions selected. There you can add up to 9 more genes, switch between the positive and negative network, and export the edge list.

Single-cell expression

Whether this gene can be visualised in the single-cell atlases of Hydractinia symbiolongicarpus, and in which cell types it is a marker. Expression is stored per cell, so the violin plot and the cell-type means are computed in the viewer — open a dataset to see them.

No single-cell dataset has been published for this species. The atlases that do exist are listed in the Cell Atlas.

Epigenetic marks

Chromatin and DNA-methylation data covering this gene. Peak calls are listed per sample with the genomic region the peak falls in; DNA methylation is listed as sample availability only, because those tables are queried by the DNA Methylation page itself.

No epigenomic data has been deposited for this species. The assays that exist across the site are described in the Epigenomic Data module.

What you can do with this gene

Every tool below opens with this gene already entered, so you land on the analysis rather than on an empty form. Links open in a new tab.

Analysis	What it does	Status
Primer design	Design PCR / qPCR primers on this gene’s sequence with the published primer3 settings. Opens with the primers already computed.	template found	open →
BLAST	Search this gene’s sequence against the CnidoSite BLAST databases. The query is filled in and the species’ database is preselected — press Run to start.	template found	open →
Expression heatmap	Draw this gene’s expression as a heatmap across all RNA-seq samples, and compare it with the genes you add next to it.	ready	open →
Gene family / orthogroup	Look this gene up in the single-copy orthogroups built from the high-quality cnidarian genomes, and see which other species carry an orthologue.		open →
Gene set analysis	Start a gene-set enrichment analysis with this gene as the seed list. Add more genes on that page before running it.		open →