Detailed information of HSymV2.0_g05.09647_t1 in Hydractinia symbiolongicarpus

Genomic Location: HiC_scaffold_5:27290812...27304977
NR annotation: XP_002163509.3, DNA-directed RNA polymerase II subunit RPB2 [Hydra vulgaris]
Species Hydractinia symbiolongicarpus · all data for this species · gene families


 Gene Structure
More details in JBrowse  Sequence
CDS
Transcript
Protein
 UniProt (Swiss-Prot top hit)
UniProt accessionDescription
A5PJW8DNA-directed RNA polymerase II subunit RPB2 OS=Bos taurus OX=9913 GN=POLR2B PE=1 SV=1
P30876DNA-directed RNA polymerase II subunit RPB2 OS=Homo sapiens OX=9606 GN=POLR2B PE=1 SV=1
Q8CFI7DNA-directed RNA polymerase II subunit RPB2 OS=Mus musculus OX=10090 GN=Polr2b PE=1 SV=2
 Gene family
Family typeMembership / link
Orthogroup (gene family)OG0001356 (this species only) · gene tree & orthology

 Pfam domain
Pfam accessionPfam nameDescriptionTypeSource
PF04561
all species →
RNA_pol_Rpb2_2RNA polymerase Rpb2, domain 2DomainInterproscan
PF00562
all species →
RNA_pol_Rpb2_6RNA polymerase Rpb2, domain 6DomainInterproscan
PF04566
all species →
RNA_pol_Rpb2_4RNA polymerase Rpb2, domain 4DomainInterproscan
PF04565
all species →
RNA_pol_Rpb2_3RNA polymerase Rpb2, domain 3DomainInterproscan
PF04563
all species →
RNA_pol_Rpb2_1RNA polymerase beta subunitFamilyInterproscan
PF04567
all species →
RNA_pol_Rpb2_5RNA polymerase Rpb2, domain 5DomainInterproscan
PF04560
all species →
RNA_pol_Rpb2_7RNA polymerase Rpb2, domain 7DomainInterproscan

 InterPro
InterPro termTypeDescriptionSource
IPR007642
all species →
DomainRNA polymerase Rpb2, domain 2Interproscan
IPR007121
all species →
Conserved_siteRNA polymerase, beta subunit, conserved siteInterproscan
IPR007120
all species →
DomainDNA-directed RNA polymerase, subunit 2, hybrid-binding domainInterproscan
IPR015712
all species →
FamilyDNA-directed RNA polymerase, subunit 2Interproscan
IPR037033
all species →
Homologous_superfamilyDNA-directed RNA polymerase, subunit 2, hybrid-binding domain superfamilyInterproscan
IPR007646
all species →
DomainRNA polymerase Rpb2, domain 4Interproscan
IPR014724
all species →
Homologous_superfamilyRNA polymerase Rpb2, OB-foldInterproscan
IPR007645
all species →
DomainRNA polymerase Rpb2, domain 3Interproscan
IPR007644
all species →
DomainRNA polymerase, beta subunit, protrusionInterproscan
IPR037034
all species →
Homologous_superfamilyRNA polymerase Rpb2, domain 2 superfamilyInterproscan
IPR007647
all species →
DomainRNA polymerase Rpb2, domain 5Interproscan
IPR007641
all species →
DomainRNA polymerase Rpb2, domain 7Interproscan

 PANTHER
PANTHER termDescriptionSource
PTHR20856
all species →
DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2Interproscan

 Gene Ontology
GO termCategoryDescriptionSource
GO:0003677
all species →
Molecular FunctionDNA bindingInterproscan
GO:0003899
all species →
Molecular FunctionDNA-directed 5'-3' RNA polymerase activityInterproscan
GO:0006351
all species →
Biological ProcessDNA-templated transcriptionInterproscan
GO:0032549
all species →
Molecular Functionribonucleoside bindingInterproscan
GO:0001055
all species →
Molecular FunctionRNA polymerase II activityInterproscan
GO:0005665
all species →
Cellular ComponentRNA polymerase II, core complexInterproscan

Search by domain instead of by gene. Any accession above (InterPro, Pfam, PANTHER, GO, KEGG) can be used as a query on the Functional Domain Search page, which searches all 148 annotated genomes at once.
 KEGG pathway
KOEnzymeEnzyme IDPathwayMap IDSource
K03010RPB2, POLR2B; DNA-directed RNA polymerase II subunit RPB2EC:2.7.7.6
DNA repair and recombination proteinsko03400deepkoala

Searching by KO or pathway ID across all species is available on the KEGG Pathway page.

Expression pattern (RNA-seq)

Transcript abundance of HSymV2.0_g05.09647_t1 across 44 RNA-seq samples of Hydractinia symbiolongicarpus. Values are TPM (transcripts per million) from the StringTie quantification; one bar is one sample, grouped and coloured by condition, sorted by expression within each group.

44Samples
44TPM > 0
3Conditions
198.7Max TPM
130.3Mean TPM

By condition

ConditionSamplesTPM > 0 Mean TPMMax TPMMean, relative to max
Whole embryo 36 36 122.19 171.91
Whole embryo · Triptolide 20 uM 4 4 176.07 198.73
Whole embryo · DMSO 0.5% 4 4 157.12 186.23

Per sample · hover a bar for the full sample record

Show the sample table (44 samples)
SRA runConditionTissueDevelopmental stage TreatmentStudyTPM
SRR24482141 Whole embryo Whole embryo 1 hpf not recorded SRP436676 171.91
SRR24482140 Whole embryo Whole embryo 2 hpf not recorded SRP436676 170.87
SRR24482143 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 170.76
SRR24482145 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 170.71
SRR24482134 Whole embryo Whole embryo 5 hpf not recorded SRP436676 169.22
SRR24482142 Whole embryo Whole embryo 30 mpf not recorded SRP436676 167.35
SRR24482177 Whole embryo Whole embryo 1 hpf not recorded SRP436676 167.03
SRR24482139 Whole embryo Whole embryo 2 hpf not recorded SRP436676 166.33
SRR24482138 Whole embryo Whole embryo 3 hpf not recorded SRP436676 165.12
SRR24482135 Whole embryo Whole embryo 4 hpf not recorded SRP436676 158.90
SRR24482144 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 158.90
SRR24482136 Whole embryo Whole embryo 4 hpf not recorded SRP436676 158.61
SRR24482133 Whole embryo Whole embryo 6 hpf not recorded SRP436676 158.09
SRR24482153 Whole embryo Whole embryo 7 hpf not recorded SRP436676 154.48
SRR24482137 Whole embryo Whole embryo 3 hpf not recorded SRP436676 152.79
SRR24482161 Whole embryo Whole embryo 7 hpf not recorded SRP436676 135.03
SRR24482165 Whole embryo Whole embryo 4 hpf not recorded SRP436676 134.73
SRR24482167 Whole embryo Whole embryo 3 hpf not recorded SRP436676 126.97
SRR24482169 Whole embryo Whole embryo 2 hpf not recorded SRP436676 123.84
SRR24482173 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 121.85
SRR24482170 Whole embryo Whole embryo 2 hpf not recorded SRP436676 119.53
SRR24482174 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 118.12
SRR24482163 Whole embryo Whole embryo 6 hpf not recorded SRP436676 117.24
SRR24482166 Whole embryo Whole embryo 4 hpf not recorded SRP436676 116.80
SRR24482171 Whole embryo Whole embryo 1 hpf not recorded SRP436676 113.38
SRR24482172 Whole embryo Whole embryo 30 mpf not recorded SRP436676 112.88
SRR24482168 Whole embryo Whole embryo 3 hpf not recorded SRP436676 111.92
SRR24482162 Whole embryo Whole embryo 7 hpf not recorded SRP436676 110.86
SRR24482176 Whole embryo Whole embryo 1 hpf not recorded SRP436676 98.79
SRR24482175 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 93.17
SRR24482157 Whole embryo Whole embryo 48 hpf not recorded SRP436676 39.35
SRR24482156 Whole embryo Whole embryo 72 hpf not recorded SRP436676 38.99
SRR24482160 Whole embryo Whole embryo 24 hpf not recorded SRP436676 36.93
SRR24482158 Whole embryo Whole embryo 48 hpf not recorded SRP436676 29.68
SRR24482159 Whole embryo Whole embryo 24 hpf not recorded SRP436676 27.61
SRR24482155 Whole embryo Whole embryo 72 hpf not recorded SRP436676 10.15
SRR24482149 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 198.73
SRR24482150 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 197.55
SRR24482146 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 154.15
SRR24482147 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 153.84
SRR24482151 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 186.23
SRR24482152 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 177.75
SRR24482154 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 138.38
SRR24482148 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 126.13

Source: CnidoSite RNA-seq expression matrices (HSYMB_TPM, StringTie quantification over 44 runs), joined to SRA sample metadata. Samples whose tissue/treatment is not recorded in the source metadata are grouped by the descriptor carried in the expression matrix itself.

Co-expression network

Genes whose expression across the transcriptome samples of Hydractinia symbiolongicarpus tracks this one. Counts are over the whole network; the network view itself draws at most 100 partners per query gene (CNIDO_NET_TOP_K), so a hub gene can show fewer edges than the number below.

DirectionPartnersMost correlated partnerPCC
Positively correlated42HSymV2.0_g11.19881_t10.979848196162688
Negatively correlated15HSymV2.0_g03.04471_t1-0.924696623118901

Open this gene in Network Analysis → The network opens with the gene already entered and both directions selected. There you can add up to 9 more genes, switch between the positive and negative network, and export the edge list.

Single-cell expression

Whether this gene can be visualised in the single-cell atlases of Hydractinia symbiolongicarpus, and in which cell types it is a marker. Expression is stored per cell, so the violin plot and the cell-type means are computed in the viewer — open a dataset to see them.

No single-cell dataset has been published for this species. The atlases that do exist are listed in the Cell Atlas.

Epigenetic marks

Chromatin and DNA-methylation data covering this gene. Peak calls are listed per sample with the genomic region the peak falls in; DNA methylation is listed as sample availability only, because those tables are queried by the DNA Methylation page itself.

No epigenomic data has been deposited for this species. The assays that exist across the site are described in the Epigenomic Data module.

What you can do with this gene

Every tool below opens with this gene already entered, so you land on the analysis rather than on an empty form. Links open in a new tab.

AnalysisWhat it doesStatus
Primer designDesign PCR / qPCR primers on this gene’s sequence with the published primer3 settings. Opens with the primers already computed.template foundopen →
BLASTSearch this gene’s sequence against the CnidoSite BLAST databases. The query is filled in and the species’ database is preselected — press Run to start.template foundopen →
Expression heatmapDraw this gene’s expression as a heatmap across all RNA-seq samples, and compare it with the genes you add next to it.readyopen →
Gene family / orthogroupLook this gene up in the single-copy orthogroups built from the high-quality cnidarian genomes, and see which other species carry an orthologue.open →
Gene set analysisStart a gene-set enrichment analysis with this gene as the seed list. Add more genes on that page before running it.open →
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