Detailed information of HSymV2.0_g05.09858_t1 in Hydractinia symbiolongicarpus

Genomic Location: HiC_scaffold_5:34208620...34210219
NR annotation: XP_046858960.1, dynein axonemal assembly factor 3-like isoform X1 [Xenia sp. Carnegie-2017]
Species Hydractinia symbiolongicarpus · all data for this species · gene families


 Gene Structure
More details in JBrowse  Sequence
CDS
Transcript
Protein
 UniProt (Swiss-Prot top hit)
UniProt accessionDescription
Q32NQ7Dynein axonemal assembly factor 3 OS=Xenopus laevis OX=8355 GN=dnaaf3 PE=2 SV=1
F6S9E6Dynein axonemal assembly factor 3 OS=Xenopus tropicalis OX=8364 GN=dnaaf3 PE=3 SV=2
F1Q7Z7Dynein axonemal assembly factor 3 OS=Danio rerio OX=7955 GN=dnaaf3 PE=2 SV=2
 Gene family
Family typeMembership / link
Orthogroup (gene family)OG0004861 (this species only) · gene tree & orthology

 Pfam domain
Pfam accessionPfam nameDescriptionTypeSource
PF14740
all species →		DUF4471Domain of unknown function (DUF4471)DomainInterproscan
PF14737
all species →		DUF4470Domain of unknown function (DUF4470)FamilyInterproscan

 InterPro
InterPro termTypeDescriptionSource
IPR028235
all species →		DomainDynein assembly factor 3, C-terminal domainInterproscan
IPR027974
all species →		DomainDomain of unknown function DUF4470Interproscan
IPR039304
all species →		FamilyDynein assembly factor 3, axonemalInterproscan

 PANTHER
PANTHER termDescriptionSource
PTHR22118
all species →		DYNEIN ASSEMBLY FACTOR 3, AXONEMALInterproscan

 Gene Ontology
GO termCategoryDescriptionSource
GO:0044458
all species →		Biological Processmotile cilium assemblyInterproscan
GO:0070286
all species →		Biological Processaxonemal dynein complex assemblyInterproscan

Search by domain instead of by gene. Any accession above (InterPro, Pfam, PANTHER, GO, KEGG) can be used as a query on the Functional Domain Search page, which searches all 148 annotated genomes at once.
 KEGG pathway
KOEnzymeEnzyme IDPathwayMap IDSource
K19752DNAAF3, PF22; dynein axonemal assembly factor 3-Cytoskeleton proteinsko04812deepkoala

Searching by KO or pathway ID across all species is available on the KEGG Pathway page.

Expression pattern (RNA-seq)

Transcript abundance of HSymV2.0_g05.09858_t1 across 44 RNA-seq samples of Hydractinia symbiolongicarpus. Values are TPM (transcripts per million) from the StringTie quantification; one bar is one sample, grouped and coloured by condition, sorted by expression within each group.

44Samples
44TPM > 0
3Conditions
113.3Max TPM
72.7Mean TPM

By condition

ConditionSamplesTPM > 0 Mean TPMMax TPMMean, relative to max
Whole embryo 36 36 72.83 113.33
Whole embryo · Triptolide 20 uM 4 4 81.96 89.64
Whole embryo · DMSO 0.5% 4 4 62.17 66.06

Per sample · hover a bar for the full sample record

Show the sample table (44 samples)
SRA runConditionTissueDevelopmental stage TreatmentStudyTPM
SRR24482175 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 113.33
SRR24482172 Whole embryo Whole embryo 30 mpf not recorded SRP436676 103.49
SRR24482141 Whole embryo Whole embryo 1 hpf not recorded SRP436676 103.10
SRR24482138 Whole embryo Whole embryo 3 hpf not recorded SRP436676 100.35
SRR24482145 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 99.96
SRR24482171 Whole embryo Whole embryo 1 hpf not recorded SRP436676 97.81
SRR24482142 Whole embryo Whole embryo 30 mpf not recorded SRP436676 95.66
SRR24482174 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 95.14
SRR24482136 Whole embryo Whole embryo 4 hpf not recorded SRP436676 94.44
SRR24482167 Whole embryo Whole embryo 3 hpf not recorded SRP436676 93.95
SRR24482173 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 93.07
SRR24482134 Whole embryo Whole embryo 5 hpf not recorded SRP436676 92.63
SRR24482168 Whole embryo Whole embryo 3 hpf not recorded SRP436676 92.23
SRR24482169 Whole embryo Whole embryo 2 hpf not recorded SRP436676 91.68
SRR24482135 Whole embryo Whole embryo 4 hpf not recorded SRP436676 89.83
SRR24482165 Whole embryo Whole embryo 4 hpf not recorded SRP436676 88.94
SRR24482140 Whole embryo Whole embryo 2 hpf not recorded SRP436676 87.87
SRR24482170 Whole embryo Whole embryo 2 hpf not recorded SRP436676 87.41
SRR24482144 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 86.15
SRR24482137 Whole embryo Whole embryo 3 hpf not recorded SRP436676 86.00
SRR24482139 Whole embryo Whole embryo 2 hpf not recorded SRP436676 82.58
SRR24482166 Whole embryo Whole embryo 4 hpf not recorded SRP436676 82.02
SRR24482177 Whole embryo Whole embryo 1 hpf not recorded SRP436676 80.09
SRR24482176 Whole embryo Whole embryo 1 hpf not recorded SRP436676 76.73
SRR24482143 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 76.53
SRR24482163 Whole embryo Whole embryo 6 hpf not recorded SRP436676 74.63
SRR24482161 Whole embryo Whole embryo 7 hpf not recorded SRP436676 61.71
SRR24482133 Whole embryo Whole embryo 6 hpf not recorded SRP436676 58.70
SRR24482153 Whole embryo Whole embryo 7 hpf not recorded SRP436676 55.64
SRR24482162 Whole embryo Whole embryo 7 hpf not recorded SRP436676 52.12
SRR24482159 Whole embryo Whole embryo 24 hpf not recorded SRP436676 9.02
SRR24482160 Whole embryo Whole embryo 24 hpf not recorded SRP436676 6.58
SRR24482155 Whole embryo Whole embryo 72 hpf not recorded SRP436676 4.09
SRR24482157 Whole embryo Whole embryo 48 hpf not recorded SRP436676 3.61
SRR24482158 Whole embryo Whole embryo 48 hpf not recorded SRP436676 2.48
SRR24482156 Whole embryo Whole embryo 72 hpf not recorded SRP436676 2.42
SRR24482150 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 89.64
SRR24482149 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 87.20
SRR24482146 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 82.92
SRR24482147 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 68.08
SRR24482152 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 66.06
SRR24482154 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 65.59
SRR24482151 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 62.66
SRR24482148 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 54.38

Source: CnidoSite RNA-seq expression matrices (HSYMB_TPM, StringTie quantification over 44 runs), joined to SRA sample metadata. Samples whose tissue/treatment is not recorded in the source metadata are grouped by the descriptor carried in the expression matrix itself.

Co-expression network

Genes whose expression across the transcriptome samples of Hydractinia symbiolongicarpus tracks this one. Counts are over the whole network; the network view itself draws at most 100 partners per query gene (CNIDO_NET_TOP_K), so a hub gene can show fewer edges than the number below.

DirectionPartnersMost correlated partnerPCC
Positively correlated46HSymV2.0_g15.25074_t10.978657746429513
Negatively correlated23HSymV2.0_g02.03321_t1-0.949660021199896

Open this gene in Network Analysis → The network opens with the gene already entered and both directions selected. There you can add up to 9 more genes, switch between the positive and negative network, and export the edge list.

Single-cell expression

Whether this gene can be visualised in the single-cell atlases of Hydractinia symbiolongicarpus, and in which cell types it is a marker. Expression is stored per cell, so the violin plot and the cell-type means are computed in the viewer — open a dataset to see them.

No single-cell dataset has been published for this species. The atlases that do exist are listed in the Cell Atlas.

Epigenetic marks

Chromatin and DNA-methylation data covering this gene. Peak calls are listed per sample with the genomic region the peak falls in; DNA methylation is listed as sample availability only, because those tables are queried by the DNA Methylation page itself.

No epigenomic data has been deposited for this species. The assays that exist across the site are described in the Epigenomic Data module.

What you can do with this gene

Every tool below opens with this gene already entered, so you land on the analysis rather than on an empty form. Links open in a new tab.

AnalysisWhat it doesStatus
Primer designDesign PCR / qPCR primers on this gene’s sequence with the published primer3 settings. Opens with the primers already computed.template foundopen →
BLASTSearch this gene’s sequence against the CnidoSite BLAST databases. The query is filled in and the species’ database is preselected — press Run to start.template foundopen →
Expression heatmapDraw this gene’s expression as a heatmap across all RNA-seq samples, and compare it with the genes you add next to it.readyopen →
Gene family / orthogroupLook this gene up in the single-copy orthogroups built from the high-quality cnidarian genomes, and see which other species carry an orthologue.open →
Gene set analysisStart a gene-set enrichment analysis with this gene as the seed list. Add more genes on that page before running it.open →
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