Detailed information of HSymV2.0_g06.10317_t1 in Hydractinia symbiolongicarpus

Genomic Location: HiC_scaffold_6:12888108...12914588
NR annotation: XP_047144990.1, WD repeat-containing protein 35 [Hydra vulgaris]
Species Hydractinia symbiolongicarpus · all data for this species · gene families


 Gene Structure
More details in JBrowse  Sequence
CDS
Transcript
Protein
 UniProt (Swiss-Prot top hit)
UniProt accessionDescription
A6N6J5WD repeat-containing protein 35 OS=Rattus norvegicus OX=10116 GN=Wdr35 PE=1 SV=1
Q8BND3WD repeat-containing protein 35 OS=Mus musculus OX=10090 GN=Wdr35 PE=1 SV=3
Q9P2L0WD repeat-containing protein 35 OS=Homo sapiens OX=9606 GN=WDR35 PE=1 SV=3
 Gene family
Family typeMembership / link
Orthogroup (gene family)OG0002208 (this species only) · gene tree & orthology

 Pfam domain
No Pfam domain signature was recorded for HSymV2.0_g06.10317_t1 in Hydractinia symbiolongicarpus.
 InterPro
InterPro termTypeDescriptionSource
IPR036322
all species →		Homologous_superfamilyWD40-repeat-containing domain superfamilyInterproscan
IPR001680
all species →		RepeatWD40 repeatInterproscan
IPR017233
all species →		FamilyWD repeat protein 35Interproscan
IPR016024
all species →		Homologous_superfamilyArmadillo-type foldInterproscan
IPR039857
all species →		FamilyIntraflagellar transport protein 122/121 homologInterproscan
IPR015943
all species →		Homologous_superfamilyWD40/YVTN repeat-like-containing domain superfamilyInterproscan

 PANTHER
PANTHER termDescriptionSource
PTHR12764
all species →		WD REPEAT DOMAIN-RELATEDInterproscan

 Gene Ontology
GO termCategoryDescriptionSource
GO:0005515
all species →		Molecular Functionprotein bindingInterproscan
GO:0030991
all species →		Cellular Componentintraciliary transport particle AInterproscan
GO:0035721
all species →		Biological Processintraciliary retrograde transportInterproscan
GO:0060271
all species →		Biological Processcilium assemblyInterproscan
GO:0061512
all species →		Biological Processprotein localization to ciliumInterproscan
GO:0097730
all species →		Cellular Componentnon-motile ciliumInterproscan
GO:1905515
all species →		Biological Processnon-motile cilium assemblyInterproscan

Search by domain instead of by gene. Any accession above (InterPro, Pfam, PANTHER, GO, KEGG) can be used as a query on the Functional Domain Search page, which searches all 148 annotated genomes at once.
 KEGG pathway
KOEnzymeEnzyme IDPathwayMap IDSource
K19674WDR35, IFT121; WD repeat-containing protein 35-Cilium and associated proteinsko03037deepkoala

Searching by KO or pathway ID across all species is available on the KEGG Pathway page.

Expression pattern (RNA-seq)

Transcript abundance of HSymV2.0_g06.10317_t1 across 44 RNA-seq samples of Hydractinia symbiolongicarpus. Values are TPM (transcripts per million) from the StringTie quantification; one bar is one sample, grouped and coloured by condition, sorted by expression within each group.

44Samples
44TPM > 0
3Conditions
129.2Max TPM
89.2Mean TPM

By condition

ConditionSamplesTPM > 0 Mean TPMMax TPMMean, relative to max
Whole embryo 36 36 87.37 122.04
Whole embryo · Triptolide 20 uM 4 4 108.19 129.17
Whole embryo · DMSO 0.5% 4 4 86.91 110.59

Per sample · hover a bar for the full sample record

Show the sample table (44 samples)
SRA runConditionTissueDevelopmental stage TreatmentStudyTPM
SRR24482138 Whole embryo Whole embryo 3 hpf not recorded SRP436676 122.04
SRR24482145 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 121.79
SRR24482142 Whole embryo Whole embryo 30 mpf not recorded SRP436676 119.46
SRR24482136 Whole embryo Whole embryo 4 hpf not recorded SRP436676 118.49
SRR24482141 Whole embryo Whole embryo 1 hpf not recorded SRP436676 118.18
SRR24482175 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 117.42
SRR24482134 Whole embryo Whole embryo 5 hpf not recorded SRP436676 117.16
SRR24482174 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 116.37
SRR24482140 Whole embryo Whole embryo 2 hpf not recorded SRP436676 113.62
SRR24482171 Whole embryo Whole embryo 1 hpf not recorded SRP436676 107.73
SRR24482172 Whole embryo Whole embryo 30 mpf not recorded SRP436676 107.18
SRR24482167 Whole embryo Whole embryo 3 hpf not recorded SRP436676 103.36
SRR24482143 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 102.32
SRR24482169 Whole embryo Whole embryo 2 hpf not recorded SRP436676 101.43
SRR24482170 Whole embryo Whole embryo 2 hpf not recorded SRP436676 100.26
SRR24482173 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 99.93
SRR24482144 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 98.39
SRR24482135 Whole embryo Whole embryo 4 hpf not recorded SRP436676 98.21
SRR24482165 Whole embryo Whole embryo 4 hpf not recorded SRP436676 95.65
SRR24482139 Whole embryo Whole embryo 2 hpf not recorded SRP436676 93.98
SRR24482168 Whole embryo Whole embryo 3 hpf not recorded SRP436676 92.89
SRR24482137 Whole embryo Whole embryo 3 hpf not recorded SRP436676 88.97
SRR24482133 Whole embryo Whole embryo 6 hpf not recorded SRP436676 86.56
SRR24482153 Whole embryo Whole embryo 7 hpf not recorded SRP436676 82.39
SRR24482166 Whole embryo Whole embryo 4 hpf not recorded SRP436676 82.08
SRR24482177 Whole embryo Whole embryo 1 hpf not recorded SRP436676 78.99
SRR24482163 Whole embryo Whole embryo 6 hpf not recorded SRP436676 77.71
SRR24482161 Whole embryo Whole embryo 7 hpf not recorded SRP436676 77.36
SRR24482176 Whole embryo Whole embryo 1 hpf not recorded SRP436676 76.64
SRR24482162 Whole embryo Whole embryo 7 hpf not recorded SRP436676 58.96
SRR24482160 Whole embryo Whole embryo 24 hpf not recorded SRP436676 44.92
SRR24482159 Whole embryo Whole embryo 24 hpf not recorded SRP436676 38.98
SRR24482157 Whole embryo Whole embryo 48 hpf not recorded SRP436676 28.99
SRR24482156 Whole embryo Whole embryo 72 hpf not recorded SRP436676 19.92
SRR24482155 Whole embryo Whole embryo 72 hpf not recorded SRP436676 18.55
SRR24482158 Whole embryo Whole embryo 48 hpf not recorded SRP436676 18.28
SRR24482149 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 129.17
SRR24482150 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 123.75
SRR24482147 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 90.00
SRR24482146 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 89.85
SRR24482151 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 110.59
SRR24482152 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 99.77
SRR24482154 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 73.84
SRR24482148 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 63.45

Source: CnidoSite RNA-seq expression matrices (HSYMB_TPM, StringTie quantification over 44 runs), joined to SRA sample metadata. Samples whose tissue/treatment is not recorded in the source metadata are grouped by the descriptor carried in the expression matrix itself.

Co-expression network

Genes whose expression across the transcriptome samples of Hydractinia symbiolongicarpus tracks this one. Counts are over the whole network; the network view itself draws at most 100 partners per query gene (CNIDO_NET_TOP_K), so a hub gene can show fewer edges than the number below.

DirectionPartnersMost correlated partnerPCC
Positively correlated24HSymV2.0_g08.14481_t10.961775525873292
Negatively correlated5HSymV2.0_g08.14378_t1-0.916118663410542

Open this gene in Network Analysis → The network opens with the gene already entered and both directions selected. There you can add up to 9 more genes, switch between the positive and negative network, and export the edge list.

Single-cell expression

Whether this gene can be visualised in the single-cell atlases of Hydractinia symbiolongicarpus, and in which cell types it is a marker. Expression is stored per cell, so the violin plot and the cell-type means are computed in the viewer — open a dataset to see them.

No single-cell dataset has been published for this species. The atlases that do exist are listed in the Cell Atlas.

Epigenetic marks

Chromatin and DNA-methylation data covering this gene. Peak calls are listed per sample with the genomic region the peak falls in; DNA methylation is listed as sample availability only, because those tables are queried by the DNA Methylation page itself.

No epigenomic data has been deposited for this species. The assays that exist across the site are described in the Epigenomic Data module.

What you can do with this gene

Every tool below opens with this gene already entered, so you land on the analysis rather than on an empty form. Links open in a new tab.

AnalysisWhat it doesStatus
Primer designDesign PCR / qPCR primers on this gene’s sequence with the published primer3 settings. Opens with the primers already computed.template foundopen →
BLASTSearch this gene’s sequence against the CnidoSite BLAST databases. The query is filled in and the species’ database is preselected — press Run to start.template foundopen →
Expression heatmapDraw this gene’s expression as a heatmap across all RNA-seq samples, and compare it with the genes you add next to it.readyopen →
Gene family / orthogroupLook this gene up in the single-copy orthogroups built from the high-quality cnidarian genomes, and see which other species carry an orthologue.open →
Gene set analysisStart a gene-set enrichment analysis with this gene as the seed list. Add more genes on that page before running it.open →
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