Detailed information of HSymV2.0_g06.10816_t1 in Hydractinia symbiolongicarpus

Genomic Location: HiC_scaffold_6:20649348...20677581
NR annotation: XP_047144545.1, E3 ubiquitin-protein ligase UBR5-like [Hydra vulgaris]
Species Hydractinia symbiolongicarpus · all data for this species · gene families


 Gene Structure
More details in JBrowse  Sequence
CDS
Transcript
Protein
 UniProt (Swiss-Prot top hit)
UniProt accessionDescription
Q80TP3E3 ubiquitin-protein ligase UBR5 OS=Mus musculus OX=10090 GN=Ubr5 PE=1 SV=2
O95071E3 ubiquitin-protein ligase UBR5 OS=Homo sapiens OX=9606 GN=UBR5 PE=1 SV=2
Q62671E3 ubiquitin-protein ligase UBR5 OS=Rattus norvegicus OX=10116 GN=Ubr5 PE=1 SV=3
 Gene family
Family typeMembership / link
Orthogroup (gene family)OG0002341 (this species only) · gene tree & orthology

 Pfam domain
Pfam accessionPfam nameDescriptionTypeSource
PF00632
all species →
HECTHECT-domain (ubiquitin-transferase)DomainInterproscan
PF00658
all species →
PABPPoly-adenylate binding protein, unique domainFamilyInterproscan
PF11547
all species →
E3_UbLigase_EDDE3 ubiquitin ligase EDDDomainInterproscan

 InterPro
InterPro termTypeDescriptionSource
IPR047503
all species →
DomainE3 ubiquitin-protein ligase UBR5, UBR-boxInterproscan
IPR002004
all species →
DomainPolyadenylate-binding protein/Hyperplastic disc proteinInterproscan
IPR003126
all species →
DomainZinc finger, UBR-typeInterproscan
IPR000569
all species →
DomainHECT domainInterproscan
IPR036053
all species →
Homologous_superfamilyPABC (PABP) domainInterproscan
IPR009091
all species →
Homologous_superfamilyRegulator of chromosome condensation 1/beta-lactamase-inhibitor protein IIInterproscan
IPR024725
all species →
DomainE3 ubiquitin-protein ligase UBR5, ubiquitin-associated domainInterproscan
IPR035983
all species →
Homologous_superfamilyHECT, E3 ligase catalytic domainInterproscan

 PANTHER
PANTHER termDescriptionSource
PTHR46276
all species →
E3 UBIQUITIN-PROTEIN LIGASE UBR5Interproscan

 Gene Ontology
GO termCategoryDescriptionSource
GO:0003723
all species →
Molecular FunctionRNA bindingInterproscan
GO:0008270
all species →
Molecular Functionzinc ion bindingInterproscan
GO:0004842
all species →
Molecular Functionubiquitin-protein transferase activityInterproscan
GO:0043130
all species →
Molecular Functionubiquitin bindingInterproscan
GO:0000209
all species →
Biological Processprotein polyubiquitinationInterproscan
GO:0005634
all species →
Cellular ComponentnucleusInterproscan
GO:0005737
all species →
Cellular ComponentcytoplasmInterproscan
GO:0034450
all species →
Molecular Functionubiquitin-ubiquitin ligase activityInterproscan
GO:0090263
all species →
Biological Processpositive regulation of canonical Wnt signaling pathwayInterproscan

Search by domain instead of by gene. Any accession above (InterPro, Pfam, PANTHER, GO, KEGG) can be used as a query on the Functional Domain Search page, which searches all 148 annotated genomes at once.
 KEGG pathway
KOEnzymeEnzyme IDPathwayMap IDSource
K10593EDD1, UBR5; E3 ubiquitin-protein ligase EDD1EC:2.3.2.26
Ubiquitin systemko04121deepkoala

Searching by KO or pathway ID across all species is available on the KEGG Pathway page.

Expression pattern (RNA-seq)

Transcript abundance of HSymV2.0_g06.10816_t1 across 44 RNA-seq samples of Hydractinia symbiolongicarpus. Values are TPM (transcripts per million) from the StringTie quantification; one bar is one sample, grouped and coloured by condition, sorted by expression within each group.

44Samples
44TPM > 0
3Conditions
123.8Max TPM
84.3Mean TPM

By condition

ConditionSamplesTPM > 0 Mean TPMMax TPMMean, relative to max
Whole embryo 36 36 81.70 123.83
Whole embryo · Triptolide 20 uM 4 4 96.00 113.72
Whole embryo · DMSO 0.5% 4 4 95.81 120.00

Per sample · hover a bar for the full sample record

Show the sample table (44 samples)
SRA runConditionTissueDevelopmental stage TreatmentStudyTPM
SRR24482153 Whole embryo Whole embryo 7 hpf not recorded SRP436676 123.83
SRR24482138 Whole embryo Whole embryo 3 hpf not recorded SRP436676 108.62
SRR24482140 Whole embryo Whole embryo 2 hpf not recorded SRP436676 107.89
SRR24482141 Whole embryo Whole embryo 1 hpf not recorded SRP436676 107.35
SRR24482142 Whole embryo Whole embryo 30 mpf not recorded SRP436676 106.77
SRR24482145 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 105.64
SRR24482133 Whole embryo Whole embryo 6 hpf not recorded SRP436676 104.92
SRR24482143 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 100.01
SRR24482134 Whole embryo Whole embryo 5 hpf not recorded SRP436676 97.85
SRR24482135 Whole embryo Whole embryo 4 hpf not recorded SRP436676 97.23
SRR24482136 Whole embryo Whole embryo 4 hpf not recorded SRP436676 96.65
SRR24482144 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 92.90
SRR24482171 Whole embryo Whole embryo 1 hpf not recorded SRP436676 90.45
SRR24482170 Whole embryo Whole embryo 2 hpf not recorded SRP436676 88.50
SRR24482175 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 87.20
SRR24482174 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 86.85
SRR24482137 Whole embryo Whole embryo 3 hpf not recorded SRP436676 84.61
SRR24482167 Whole embryo Whole embryo 3 hpf not recorded SRP436676 84.18
SRR24482139 Whole embryo Whole embryo 2 hpf not recorded SRP436676 83.31
SRR24482161 Whole embryo Whole embryo 7 hpf not recorded SRP436676 83.16
SRR24482169 Whole embryo Whole embryo 2 hpf not recorded SRP436676 82.18
SRR24482173 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 81.06
SRR24482177 Whole embryo Whole embryo 1 hpf not recorded SRP436676 80.36
SRR24482165 Whole embryo Whole embryo 4 hpf not recorded SRP436676 78.70
SRR24482172 Whole embryo Whole embryo 30 mpf not recorded SRP436676 76.11
SRR24482162 Whole embryo Whole embryo 7 hpf not recorded SRP436676 73.13
SRR24482168 Whole embryo Whole embryo 3 hpf not recorded SRP436676 72.02
SRR24482163 Whole embryo Whole embryo 6 hpf not recorded SRP436676 68.07
SRR24482157 Whole embryo Whole embryo 48 hpf not recorded SRP436676 66.59
SRR24482156 Whole embryo Whole embryo 72 hpf not recorded SRP436676 63.02
SRR24482176 Whole embryo Whole embryo 1 hpf not recorded SRP436676 62.39
SRR24482166 Whole embryo Whole embryo 4 hpf not recorded SRP436676 58.36
SRR24482160 Whole embryo Whole embryo 24 hpf not recorded SRP436676 46.77
SRR24482158 Whole embryo Whole embryo 48 hpf not recorded SRP436676 41.19
SRR24482159 Whole embryo Whole embryo 24 hpf not recorded SRP436676 36.40
SRR24482155 Whole embryo Whole embryo 72 hpf not recorded SRP436676 17.02
SRR24482149 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 113.72
SRR24482150 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 109.99
SRR24482147 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 83.75
SRR24482146 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 76.56
SRR24482151 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 120.00
SRR24482152 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 115.68
SRR24482154 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 76.60
SRR24482148 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 70.94

Source: CnidoSite RNA-seq expression matrices (HSYMB_TPM, StringTie quantification over 44 runs), joined to SRA sample metadata. Samples whose tissue/treatment is not recorded in the source metadata are grouped by the descriptor carried in the expression matrix itself.

Co-expression network

Genes whose expression across the transcriptome samples of Hydractinia symbiolongicarpus tracks this one. Counts are over the whole network; the network view itself draws at most 100 partners per query gene (CNIDO_NET_TOP_K), so a hub gene can show fewer edges than the number below.

DirectionPartnersMost correlated partnerPCC
Positively correlated8HSymV2.0_g03.05833_t10.926559430114896
Negatively correlated12HSymV2.0_g07.12141_t1-0.836387441195307

Open this gene in Network Analysis → The network opens with the gene already entered and both directions selected. There you can add up to 9 more genes, switch between the positive and negative network, and export the edge list.

Single-cell expression

Whether this gene can be visualised in the single-cell atlases of Hydractinia symbiolongicarpus, and in which cell types it is a marker. Expression is stored per cell, so the violin plot and the cell-type means are computed in the viewer — open a dataset to see them.

No single-cell dataset has been published for this species. The atlases that do exist are listed in the Cell Atlas.

Epigenetic marks

Chromatin and DNA-methylation data covering this gene. Peak calls are listed per sample with the genomic region the peak falls in; DNA methylation is listed as sample availability only, because those tables are queried by the DNA Methylation page itself.

No epigenomic data has been deposited for this species. The assays that exist across the site are described in the Epigenomic Data module.

What you can do with this gene

Every tool below opens with this gene already entered, so you land on the analysis rather than on an empty form. Links open in a new tab.

AnalysisWhat it doesStatus
Primer designDesign PCR / qPCR primers on this gene’s sequence with the published primer3 settings. Opens with the primers already computed.template foundopen →
BLASTSearch this gene’s sequence against the CnidoSite BLAST databases. The query is filled in and the species’ database is preselected — press Run to start.template foundopen →
Expression heatmapDraw this gene’s expression as a heatmap across all RNA-seq samples, and compare it with the genes you add next to it.readyopen →
Gene family / orthogroupLook this gene up in the single-copy orthogroups built from the high-quality cnidarian genomes, and see which other species carry an orthologue.open →
Gene set analysisStart a gene-set enrichment analysis with this gene as the seed list. Add more genes on that page before running it.open →
TOP