Detailed information of HSymV2.0_g08.13409_t1 in Hydractinia symbiolongicarpus

Genomic Location: HiC_scaffold_8:8935839...8957649
NR annotation: NP_001267868.1, carbamoyl-phosphate synthetase/aspartate transcarbamoylase/dihydroorotase [Hydra vulgaris]
Species Hydractinia symbiolongicarpus · all data for this species · gene families


 Gene Structure
More details in JBrowse  Sequence
CDS
Transcript
Protein
 UniProt (Swiss-Prot top hit)
UniProt accessionDescription
Q91437Multifunctional protein CAD OS=Squalus acanthias OX=7797 GN=CAD PE=2 SV=1
P27708Multifunctional protein CAD OS=Homo sapiens OX=9606 GN=CAD PE=1 SV=3
B2RQC6Multifunctional protein CAD OS=Mus musculus OX=10090 GN=Cad PE=1 SV=1
 Gene family
Family typeMembership / link
Orthogroup (gene family)OG0001105 (this species only) · gene tree & orthology

 Pfam domain
Pfam accessionPfam nameDescriptionTypeSource
PF02786
all species →
CPSase_L_D2Carbamoyl-phosphate synthase L chain, ATP binding domainDomainInterproscan
PF02729
all species →
OTCace_NAspartate/ornithine carbamoyltransferase, carbamoyl-P binding domainDomainInterproscan
PF02787
all species →
CPSase_L_D3Carbamoyl-phosphate synthetase large chain, oligomerisation domainDomainInterproscan
PF01979
all species →
Amidohydro_1Amidohydrolase familyDomainInterproscan
PF00117
all species →
GATaseGlutamine amidotransferase class-IDomainInterproscan
PF00185
all species →
OTCaceAspartate/ornithine carbamoyltransferase, Asp/Orn binding domainDomainInterproscan
PF02142
all species →
MGSMGS-like domainDomainInterproscan
PF00988
all species →
CPSase_sm_chainCarbamoyl-phosphate synthase small chain, CPSase domainDomainInterproscan

 InterPro
InterPro termTypeDescriptionSource
IPR002474
all species →
DomainCarbamoyl-phosphate synthase small subunit, N-terminal domainInterproscan
IPR036901
all species →
Homologous_superfamilyAspartate/ornithine carbamoyltransferase superfamilyInterproscan
IPR002082
all species →
FamilyAspartate carbamoyltransferaseInterproscan
IPR005480
all species →
DomainCarbamoyl-phosphate synthetase, large subunit oligomerisation domainInterproscan
IPR011761
all species →
DomainATP-grasp foldInterproscan
IPR036897
all species →
Homologous_superfamilyCarbamoyl-phosphate synthetase, large subunit oligomerisation domain superfamilyInterproscan
IPR005479
all species →
DomainCarbamoyl-phosphate synthetase large subunit-like, ATP-binding domainInterproscan
IPR036480
all species →
Homologous_superfamilyCarbamoyl-phosphate synthase small subunit, N-terminal domain superfamilyInterproscan
IPR029062
all species →
Homologous_superfamilyClass I glutamine amidotransferase-likeInterproscan
IPR006132
all species →
DomainAspartate/ornithine carbamoyltransferase, carbamoyl-P bindingInterproscan
IPR016185
all species →
Homologous_superfamilyPre-ATP-grasp domain superfamilyInterproscan
IPR013815
all species →
Homologous_superfamilyATP-grasp fold, subdomain 1Interproscan
IPR005483
all species →
DomainCarbamoyl-phosphate synthase large subunit, CPSase domainInterproscan
IPR006680
all species →
DomainAmidohydrolase-relatedInterproscan
IPR006275
all species →
FamilyCarbamoyl-phosphate synthase, large subunitInterproscan
IPR017926
all species →
DomainGlutamine amidotransferaseInterproscan
IPR006130
all species →
FamilyAspartate/ornithine carbamoyltransferaseInterproscan
IPR006131
all species →
DomainAspartate/ornithine carbamoyltransferase, Asp/Orn-binding domainInterproscan
IPR035686
all species →
DomainCarbamoyl-phosphate synthase small subunit, GATase1 domainInterproscan
IPR036914
all species →
Homologous_superfamilyMethylglyoxal synthase-like domain superfamilyInterproscan
IPR032466
all species →
Homologous_superfamilyMetal-dependent hydrolaseInterproscan
IPR011607
all species →
DomainMethylglyoxal synthase-like domainInterproscan
IPR011059
all species →
Homologous_superfamilyMetal-dependent hydrolase, composite domain superfamilyInterproscan
IPR002195
all species →
Conserved_siteDihydroorotase, conserved siteInterproscan
IPR006274
all species →
FamilyCarbamoyl-phosphate synthase, small subunitInterproscan

 PANTHER
PANTHER termDescriptionSource
PTHR11405
all species →
CARBAMOYLTRANSFERASE FAMILY MEMBERInterproscan

 Gene Ontology
GO termCategoryDescriptionSource
GO:0006520
all species →
Biological Processamino acid metabolic processInterproscan
GO:0016597
all species →
Molecular Functionamino acid bindingInterproscan
GO:0016743
all species →
Molecular Functioncarboxyl- or carbamoyltransferase activityInterproscan
GO:0004070
all species →
Molecular Functionaspartate carbamoyltransferase activityInterproscan
GO:0006207
all species →
Biological Process'de novo' pyrimidine nucleobase biosynthetic processInterproscan
GO:0005524
all species →
Molecular FunctionATP bindingInterproscan
GO:0046872
all species →
Molecular Functionmetal ion bindingInterproscan
GO:0006807
all species →
Biological Processobsolete nitrogen compound metabolic processInterproscan
GO:0016787
all species →
Molecular Functionhydrolase activityInterproscan
GO:0004088
all species →
Molecular Functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activityInterproscan
GO:0004151
all species →
Molecular Functiondihydroorotase activityInterproscan
GO:0005737
all species →
Cellular ComponentcytoplasmInterproscan
GO:0005829
all species →
Cellular ComponentcytosolInterproscan
GO:0006228
all species →
Biological ProcessUTP biosynthetic processInterproscan
GO:0006541
all species →
Biological Processglutamine metabolic processInterproscan
GO:0019240
all species →
Biological Processcitrulline biosynthetic processInterproscan
GO:0016810
all species →
Molecular Functionhydrolase activity, acting on carbon-nitrogen (but not peptide) bondsInterproscan
GO:0016812
all species →
Molecular Functionhydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidesInterproscan

Search by domain instead of by gene. Any accession above (InterPro, Pfam, PANTHER, GO, KEGG) can be used as a query on the Functional Domain Search page, which searches all 148 annotated genomes at once.
 KEGG pathway
KOEnzymeEnzyme IDPathwayMap IDSource
K11540CAD; carbamoyl-phosphate synthase / aspartate carbamoyltransferase / dihydroorotaseEC:6.3.5.5
EC:2.1.3.2
EC:3.5.2.3
Peptidases and inhibitorsko01002deepkoala

Searching by KO or pathway ID across all species is available on the KEGG Pathway page.

Expression pattern (RNA-seq)

Transcript abundance of HSymV2.0_g08.13409_t1 across 44 RNA-seq samples of Hydractinia symbiolongicarpus. Values are TPM (transcripts per million) from the StringTie quantification; one bar is one sample, grouped and coloured by condition, sorted by expression within each group.

44Samples
44TPM > 0
3Conditions
145.2Max TPM
90.9Mean TPM

By condition

ConditionSamplesTPM > 0 Mean TPMMax TPMMean, relative to max
Whole embryo 36 36 86.46 139.34
Whole embryo · Triptolide 20 uM 4 4 117.22 145.23
Whole embryo · DMSO 0.5% 4 4 104.05 142.11

Per sample · hover a bar for the full sample record

Show the sample table (44 samples)
SRA runConditionTissueDevelopmental stage TreatmentStudyTPM
SRR24482133 Whole embryo Whole embryo 6 hpf not recorded SRP436676 139.34
SRR24482153 Whole embryo Whole embryo 7 hpf not recorded SRP436676 136.24
SRR24482141 Whole embryo Whole embryo 1 hpf not recorded SRP436676 125.87
SRR24482145 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 123.44
SRR24482138 Whole embryo Whole embryo 3 hpf not recorded SRP436676 122.68
SRR24482134 Whole embryo Whole embryo 5 hpf not recorded SRP436676 121.92
SRR24482139 Whole embryo Whole embryo 2 hpf not recorded SRP436676 121.43
SRR24482140 Whole embryo Whole embryo 2 hpf not recorded SRP436676 121.26
SRR24482143 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 120.21
SRR24482144 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 119.43
SRR24482142 Whole embryo Whole embryo 30 mpf not recorded SRP436676 115.85
SRR24482136 Whole embryo Whole embryo 4 hpf not recorded SRP436676 115.74
SRR24482177 Whole embryo Whole embryo 1 hpf not recorded SRP436676 111.71
SRR24482135 Whole embryo Whole embryo 4 hpf not recorded SRP436676 110.23
SRR24482137 Whole embryo Whole embryo 3 hpf not recorded SRP436676 109.90
SRR24482174 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 96.73
SRR24482167 Whole embryo Whole embryo 3 hpf not recorded SRP436676 96.63
SRR24482171 Whole embryo Whole embryo 1 hpf not recorded SRP436676 96.16
SRR24482175 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 92.58
SRR24482165 Whole embryo Whole embryo 4 hpf not recorded SRP436676 90.46
SRR24482173 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 89.95
SRR24482169 Whole embryo Whole embryo 2 hpf not recorded SRP436676 88.78
SRR24482170 Whole embryo Whole embryo 2 hpf not recorded SRP436676 86.62
SRR24482161 Whole embryo Whole embryo 7 hpf not recorded SRP436676 85.93
SRR24482172 Whole embryo Whole embryo 30 mpf not recorded SRP436676 84.33
SRR24482168 Whole embryo Whole embryo 3 hpf not recorded SRP436676 75.92
SRR24482176 Whole embryo Whole embryo 1 hpf not recorded SRP436676 71.48
SRR24482166 Whole embryo Whole embryo 4 hpf not recorded SRP436676 66.10
SRR24482163 Whole embryo Whole embryo 6 hpf not recorded SRP436676 64.17
SRR24482162 Whole embryo Whole embryo 7 hpf not recorded SRP436676 62.65
SRR24482157 Whole embryo Whole embryo 48 hpf not recorded SRP436676 13.50
SRR24482160 Whole embryo Whole embryo 24 hpf not recorded SRP436676 8.41
SRR24482156 Whole embryo Whole embryo 72 hpf not recorded SRP436676 7.58
SRR24482159 Whole embryo Whole embryo 24 hpf not recorded SRP436676 7.18
SRR24482158 Whole embryo Whole embryo 48 hpf not recorded SRP436676 6.49
SRR24482155 Whole embryo Whole embryo 72 hpf not recorded SRP436676 5.76
SRR24482150 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 145.23
SRR24482149 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 142.87
SRR24482147 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 96.47
SRR24482146 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 84.31
SRR24482151 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 142.11
SRR24482152 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 135.88
SRR24482154 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 74.03
SRR24482148 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 64.18

Source: CnidoSite RNA-seq expression matrices (HSYMB_TPM, StringTie quantification over 44 runs), joined to SRA sample metadata. Samples whose tissue/treatment is not recorded in the source metadata are grouped by the descriptor carried in the expression matrix itself.

Co-expression network

Genes whose expression across the transcriptome samples of Hydractinia symbiolongicarpus tracks this one. Counts are over the whole network; the network view itself draws at most 100 partners per query gene (CNIDO_NET_TOP_K), so a hub gene can show fewer edges than the number below.

DirectionPartnersMost correlated partnerPCC
Positively correlated38HSymV2.0_g10.19211_t10.974110508741105
Negatively correlated9HSymV2.0_g03.04525_t1-0.940609168054129

Open this gene in Network Analysis → The network opens with the gene already entered and both directions selected. There you can add up to 9 more genes, switch between the positive and negative network, and export the edge list.

Single-cell expression

Whether this gene can be visualised in the single-cell atlases of Hydractinia symbiolongicarpus, and in which cell types it is a marker. Expression is stored per cell, so the violin plot and the cell-type means are computed in the viewer — open a dataset to see them.

No single-cell dataset has been published for this species. The atlases that do exist are listed in the Cell Atlas.

Epigenetic marks

Chromatin and DNA-methylation data covering this gene. Peak calls are listed per sample with the genomic region the peak falls in; DNA methylation is listed as sample availability only, because those tables are queried by the DNA Methylation page itself.

No epigenomic data has been deposited for this species. The assays that exist across the site are described in the Epigenomic Data module.

What you can do with this gene

Every tool below opens with this gene already entered, so you land on the analysis rather than on an empty form. Links open in a new tab.

AnalysisWhat it doesStatus
Primer designDesign PCR / qPCR primers on this gene’s sequence with the published primer3 settings. Opens with the primers already computed.template foundopen →
BLASTSearch this gene’s sequence against the CnidoSite BLAST databases. The query is filled in and the species’ database is preselected — press Run to start.template foundopen →
Expression heatmapDraw this gene’s expression as a heatmap across all RNA-seq samples, and compare it with the genes you add next to it.readyopen →
Gene family / orthogroupLook this gene up in the single-copy orthogroups built from the high-quality cnidarian genomes, and see which other species carry an orthologue.open →
Gene set analysisStart a gene-set enrichment analysis with this gene as the seed list. Add more genes on that page before running it.open →
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