Gene detail

Detailed information of HSymV2.0_g09.15191_t1 in Hydractinia symbiolongicarpus

Genomic Location: HiC_scaffold_9:5945780...5969043
NR annotation: XP_002158978.2, fermitin family homolog 2 [Hydra vulgaris]
Species Hydractinia symbiolongicarpus · all data for this species · gene families

Gene Structure

More details in JBrowse

Sequence

CDS

HSymV2.0_g09.15191_t1

ATGCTGGAAATTGTTGAGAAATTAGATGTTACAGCAGATTATTCTGATCATGCACTTTGGTGGCCTGAAAAAAAAATTTGGATGCAGAAACCACGTCTATCCCTAGCCTCCTATGGCATGACCTATGACACACAACTTCAATTCACACCACAGCATAAAAGTGTAAGACTTCAAATGCCTGATCTTCAGATATTAGATATGAGAGTAAACTTTGCTGTTGATGTATTTCATACTGTATCAGAATTATGTGCAGAACTTGGCATAAGACACCCAGAAGAACTATCCTTAATGAGACCTTTTGAAACTGGTACTGCTAAAAAACGAAGGAAAGGGAAAAAAGGTTCTGGATCTGTTGGTTCAGATGAGACAGGTTCTCAGGGCAGTGTTGGAAATGGTACTCTTAATAGATctacactacaaacacctacaactccagcaccaactacTTCACCTGTCTCTACTGGTGGAAGATCAGGCTCAGATTATTCTTATTCTAGTAGTGATGCTCTTAACCCATACACCACAGCATTGTCTCCCATGTTAGCTCACAGTCCCAATACGGTCACCCCTGAACAACTAGATGGTATTGGACGCGGAAAACCACTCGTAGAACGTGCAACCTTTAATACTGGCTGGTTTGCATCTTCTGAATCACTTATGCAACAGGGAGTAAAAGAATATGACAACTTGTTACTGAGGTTTAAATACTACAACTTCTTTGACTTAAATCCCAAGATTGATGAGGTGCGAATCAACCAAATTTACGAGCAAGCTAAATGGCAAGTACTAACAGAAGAAATCGAATGCACAGAAGAAGAAGCGATCACCTTTGCTGCATTACAATTCCAAGTAAAAGTCGCTGCTCAAAACCCACAAAACTCATCGGCCACTGAAGAAGTTGATGATATAGAGTCAGCCCTCAATGAATTGCAGATGACATTGGAGGGTACAAGTCAACAAAACACGTCTACTGGAGTTAGTCAACCTTCGCAACGACCAGCTCTTTCACATATCCCTGAAATGAAAGACAATCTAAACTTAATAAAGTCCAAAAGTTTTGGCATGAAAAGTGTCAAGAGCTACTATTTTCTATTTAAAGATACGCATATATCTTATTATAAACAGCAATCAGAATCTATGGCTCAACCGATACAAAAATTCAACCTAAAAGGTTGTGAGGTATTTCCGGAAGTTAACATCAGCAAAGATAAATATCATATCAAATTAAGAATACCAGGTCCTGACATGTTTGAAGTAGAGCTTGGATGTAACTCACCAGAACAGTATGTCAAATGGATGGCTGCATGTCGCCTCGCATCAAAGGGAAAAACTATGGCTGACAACACTTATGAAATGGAAATGTCTGGTGTGCGAACCTTCTTAAGTATGCAAAGTGATGGTGGTTTAGCCAGAGACGATACAGATTACTCTTTAGGAGAACATGCTGGAATGCAACCAGAAGACTTTGTTCCACAAAGAATTTTATCAAAGTACAAGAGTAAACAGATCGCGGCTCGGATTCTGGAATCACACTCGTCGTTTACCAAGCTTGGCCTTTTAGAAACTAAAATGAATTTCATTCGCCAGTGGCAGTCACTACCAGATTTTGGTATTACGAAATTCAATGCGAGATTTAGAGAGTCTAAGAAGAAACAGGAGATAGTTGGTATAGGGACCAACAAAATTACCCGTTTCGATGTCAGCTCCCGTCAAGTCGTGAAACAATGGCGGTACAGCTCCATGAAGAATTGGAATGTGAACTGGGAAACGCGTGAAATGATTATCTCATGCGAAGGAGATAACAACACTGTCATATTCTCCCTAGTAGGTGGAGATATTAGATTGGCGCACGAGTTTATTGGAGGATCATTTCACAAGTTTCAGCCCGACGCTTTGAAAGGACACAACTCTCACAACAACATCATGGATCCTTCTCTTTTGAAAGGTCTCAATGCATTTAAAAAGAAAAGTTTAGCTACTCCTGTAGTTGAATCGCGTCGAAATGTAAAGACAGATGCAAACAACGTACAGAAAGAACGAACAAGATCGAAAAAGAGAAGACCAGAGTTACCACCTCAGCTTTTACAGCAAGCAAAGAAACAAGCAACCAGTCAAATTGTCAATTATAAGATACACACACAAATTAAACAAAAAAGTCGTTTTTCAATCCTCTCGTCGATAGTTGATTTACTTAAGAGTAAATACCAGAATAGAGATTTTCAAGTGTACACATTAGAACAGTTACTAGACCTGACCCACAACACAGATATAAAAGACCATAATAAACAGTGGCTTGCTGAAGCGTTGAAAGACAATCCCAAGATTGCTTTTAAAGATATACACTACTCCTTTAAGCCAAAATATGCACTGAGAGATCGCAAATCGTTAGTGAGACTGTTGGAAAAACACGAGCAAAATGGTTTAGGTGGTGTGCTGTTAGACGATATACGAGAAGGATTGCCAAACGCTGATGAAATTATAAAAGCGGTTAGCGATAAAGTGACCTTTGTAACGCGAAGTAACGACAAGAAAGCGATTTTATTTTATTACGATTCTTCATTTCATTCGCAAGTAGATGATGAGTTTCAGAAACATTGGAGAAGCGTAGCTGTCGAAGGGTTAGCCGAAGCGGATATCGAACGGTACTTGAATAGCAGTGGTATTACGTCCATGCAGGGTGTAGCTCCTACACAGAAGCGACACGAAAAGAAACGGGGTAACAAACGTCGCAAGAACATGAAACTGATGAACACTCATTTAGGAGGAGACATGTTAAAGGATTATTCGGAGTCAGGAAAACATTGA

Transcript

HSymV2.0_g09.15191_t1

Protein

UniProt (Swiss-Prot top hit)

UniProt accession	Description
Q96AC1	Fermitin family homolog 2 OS=Homo sapiens OX=9606 GN=FERMT2 PE=1 SV=1
Q8CIB5	Fermitin family homolog 2 OS=Mus musculus OX=10090 GN=Fermt2 PE=1 SV=1
Q9BQL6	Fermitin family homolog 1 OS=Homo sapiens OX=9606 GN=FERMT1 PE=1 SV=1

Gene family

Family type	Membership / link
Orthogroup (gene family)	OG0006404 (this species only) · gene tree & orthology

Pfam domain

Pfam accession	Pfam name	Description	Type	Source
PF18124 all species →	Kindlin_2_N	Kindlin-2 N-terminal domain	Domain	Interproscan
PF00373 all species →	FERM_M	FERM central domain	Domain	Interproscan
PF00169 all species →	PH	PH domain	Domain	Interproscan
PF02186 all species →	TFIIE_beta	TFIIE beta subunit core domain	Domain	Interproscan
PF18121 all species →	TFA2_Winged_2	TFA2 Winged helix domain 2	Domain	Interproscan

InterPro

InterPro term	Type	Description	Source
IPR036388 all species →	Homologous_superfamily	Winged helix-like DNA-binding domain superfamily	Interproscan
IPR035963 all species →	Homologous_superfamily	FERM superfamily, second domain	Interproscan
IPR036390 all species →	Homologous_superfamily	Winged helix DNA-binding domain superfamily	Interproscan
IPR001849 all species →	Domain	Pleckstrin homology domain	Interproscan
IPR040790 all species →	Domain	Kindlin-2, N-terminal	Interproscan
IPR019748 all species →	Domain	FERM central domain	Interproscan
IPR014352 all species →	Homologous_superfamily	FERM/acyl-CoA-binding protein superfamily	Interproscan
IPR011993 all species →	Homologous_superfamily	PH-like domain superfamily	Interproscan
IPR037843 all species →	Family	Kindlin/fermitin	Interproscan
IPR003166 all species →	Domain	Transcription factor TFIIE beta subunit, DNA-binding domain	Interproscan
IPR040501 all species →	Domain	TFA2, Winged helix domain 2	Interproscan
IPR019749 all species →	Domain	Band 4.1 domain	Interproscan

PANTHER

PANTHER term	Description	Source
PTHR16160 all species →	FERMITIN 2-RELATED	Interproscan

Gene Ontology

GO term	Category	Description	Source
GO:0005178 all species →	Molecular Function	integrin binding	Interproscan
GO:0007160 all species →	Biological Process	cell-matrix adhesion	Interproscan
GO:0007229 all species →	Biological Process	integrin-mediated signaling pathway	Interproscan
GO:0030055 all species →	Cellular Component	cell-substrate junction	Interproscan
GO:0006367 all species →	Biological Process	transcription initiation at RNA polymerase II promoter	Interproscan

Search by domain instead of by gene. Any accession above (InterPro, Pfam, PANTHER, GO, KEGG) can be used as a query on the Functional Domain Search page, which searches all 148 annotated genomes at once.

KEGG pathway

KO	Enzyme	Enzyme ID	Pathway	Map ID	Source
K17083	FERMT2, KIND2; kindlin 2	-	Domain-containing proteins not elsewhere classified	ko04990	deepkoala

Searching by KO or pathway ID across all species is available on the KEGG Pathway page.

Expression pattern (RNA-seq)

Transcript abundance of HSymV2.0_g09.15191_t1 across 44 RNA-seq samples of Hydractinia symbiolongicarpus. Values are TPM (transcripts per million) from the StringTie quantification; one bar is one sample, grouped and coloured by condition, sorted by expression within each group.

44Samples

44TPM > 0

3Conditions

330.1Max TPM

191.7Mean TPM

By condition

Condition	Samples	TPM > 0	Mean TPM	Max TPM
Whole embryo	36	36	173.21	330.13
Whole embryo · Triptolide 20 uM	4	4	254.91	281.86
Whole embryo · DMSO 0.5%	4	4	294.81	306.75

Per sample · hover a bar for the full sample record

Show the sample table (44 samples)

SRA run	Condition	Tissue	Developmental stage	Treatment	Study	TPM
SRR24482162	Whole embryo	Whole embryo	7 hpf	not recorded	SRP436676	330.13
SRR24482153	Whole embryo	Whole embryo	7 hpf	not recorded	SRP436676	319.41
SRR24482161	Whole embryo	Whole embryo	7 hpf	not recorded	SRP436676	308.09
SRR24482133	Whole embryo	Whole embryo	6 hpf	not recorded	SRP436676	258.66
SRR24482177	Whole embryo	Whole embryo	1 hpf	not recorded	SRP436676	254.39
SRR24482163	Whole embryo	Whole embryo	6 hpf	not recorded	SRP436676	222.45
SRR24482165	Whole embryo	Whole embryo	4 hpf	not recorded	SRP436676	217.09
SRR24482167	Whole embryo	Whole embryo	3 hpf	not recorded	SRP436676	205.91
SRR24482144	Whole embryo	Whole embryo	Unfertilized egg	not recorded	SRP436676	198.29
SRR24482139	Whole embryo	Whole embryo	2 hpf	not recorded	SRP436676	194.59
SRR24482137	Whole embryo	Whole embryo	3 hpf	not recorded	SRP436676	191.49
SRR24482166	Whole embryo	Whole embryo	4 hpf	not recorded	SRP436676	190.67
SRR24482143	Whole embryo	Whole embryo	Unfertilized egg	not recorded	SRP436676	188.94
SRR24482135	Whole embryo	Whole embryo	4 hpf	not recorded	SRP436676	188.63
SRR24482140	Whole embryo	Whole embryo	2 hpf	not recorded	SRP436676	186.40
SRR24482134	Whole embryo	Whole embryo	5 hpf	not recorded	SRP436676	182.98
SRR24482142	Whole embryo	Whole embryo	30 mpf	not recorded	SRP436676	179.50
SRR24482145	Whole embryo	Whole embryo	Unfertilized egg	not recorded	SRP436676	178.86
SRR24482169	Whole embryo	Whole embryo	2 hpf	not recorded	SRP436676	178.27
SRR24482168	Whole embryo	Whole embryo	3 hpf	not recorded	SRP436676	176.74
SRR24482138	Whole embryo	Whole embryo	3 hpf	not recorded	SRP436676	175.41
SRR24482141	Whole embryo	Whole embryo	1 hpf	not recorded	SRP436676	174.25
SRR24482136	Whole embryo	Whole embryo	4 hpf	not recorded	SRP436676	170.17
SRR24482170	Whole embryo	Whole embryo	2 hpf	not recorded	SRP436676	162.12
SRR24482160	Whole embryo	Whole embryo	24 hpf	not recorded	SRP436676	153.31
SRR24482171	Whole embryo	Whole embryo	1 hpf	not recorded	SRP436676	145.70
SRR24482174	Whole embryo	Whole embryo	Unfertilized egg	not recorded	SRP436676	141.36
SRR24482173	Whole embryo	Whole embryo	Unfertilized egg	not recorded	SRP436676	136.51
SRR24482172	Whole embryo	Whole embryo	30 mpf	not recorded	SRP436676	127.25
SRR24482176	Whole embryo	Whole embryo	1 hpf	not recorded	SRP436676	122.74
SRR24482175	Whole embryo	Whole embryo	Unfertilized egg	not recorded	SRP436676	121.36
SRR24482159	Whole embryo	Whole embryo	24 hpf	not recorded	SRP436676	82.36
SRR24482157	Whole embryo	Whole embryo	48 hpf	not recorded	SRP436676	63.94
SRR24482158	Whole embryo	Whole embryo	48 hpf	not recorded	SRP436676	41.67
SRR24482155	Whole embryo	Whole embryo	72 hpf	not recorded	SRP436676	35.96
SRR24482156	Whole embryo	Whole embryo	72 hpf	not recorded	SRP436676	29.95
SRR24482147	Whole embryo · Triptolide 20 uM	Whole embryo	7 hpf	Triptolide 20 uM	SRP436676	281.86
SRR24482149	Whole embryo · Triptolide 20 uM	Whole embryo	7 hpf	Triptolide 20 uM	SRP436676	253.18
SRR24482150	Whole embryo · Triptolide 20 uM	Whole embryo	7 hpf	Triptolide 20 uM	SRP436676	248.53
SRR24482146	Whole embryo · Triptolide 20 uM	Whole embryo	7 hpf	Triptolide 20 uM	SRP436676	236.08
SRR24482151	Whole embryo · DMSO 0.5%	Whole embryo	7 hpf	DMSO 0.5%	SRP436676	306.75
SRR24482152	Whole embryo · DMSO 0.5%	Whole embryo	7 hpf	DMSO 0.5%	SRP436676	299.58
SRR24482148	Whole embryo · DMSO 0.5%	Whole embryo	7 hpf	DMSO 0.5%	SRP436676	295.80
SRR24482154	Whole embryo · DMSO 0.5%	Whole embryo	7 hpf	DMSO 0.5%	SRP436676	277.11

Source: CnidoSite RNA-seq expression matrices (HSYMB_TPM, StringTie quantification over 44 runs), joined to SRA sample metadata. Samples whose tissue/treatment is not recorded in the source metadata are grouped by the descriptor carried in the expression matrix itself.

Co-expression network

Genes whose expression across the transcriptome samples of Hydractinia symbiolongicarpus tracks this one. Counts are over the whole network; the network view itself draws at most 100 partners per query gene (CNIDO_NET_TOP_K), so a hub gene can show fewer edges than the number below.

Direction	Partners	Most correlated partner	PCC
Positively correlated	34	HSymV2.0_g08.13169_t1	0.963070337978008
Negatively correlated	17	HSymV2.0_g09.15197_t1	-0.926090870957235

Open this gene in Network Analysis → The network opens with the gene already entered and both directions selected. There you can add up to 9 more genes, switch between the positive and negative network, and export the edge list.

Single-cell expression

Whether this gene can be visualised in the single-cell atlases of Hydractinia symbiolongicarpus, and in which cell types it is a marker. Expression is stored per cell, so the violin plot and the cell-type means are computed in the viewer — open a dataset to see them.

No single-cell dataset has been published for this species. The atlases that do exist are listed in the Cell Atlas.

Epigenetic marks

Chromatin and DNA-methylation data covering this gene. Peak calls are listed per sample with the genomic region the peak falls in; DNA methylation is listed as sample availability only, because those tables are queried by the DNA Methylation page itself.

No epigenomic data has been deposited for this species. The assays that exist across the site are described in the Epigenomic Data module.

What you can do with this gene

Every tool below opens with this gene already entered, so you land on the analysis rather than on an empty form. Links open in a new tab.

Analysis	What it does	Status
Primer design	Design PCR / qPCR primers on this gene’s sequence with the published primer3 settings. Opens with the primers already computed.	template found	open →
BLAST	Search this gene’s sequence against the CnidoSite BLAST databases. The query is filled in and the species’ database is preselected — press Run to start.	template found	open →
Expression heatmap	Draw this gene’s expression as a heatmap across all RNA-seq samples, and compare it with the genes you add next to it.	ready	open →
Gene family / orthogroup	Look this gene up in the single-copy orthogroups built from the high-quality cnidarian genomes, and see which other species carry an orthologue.		open →
Gene set analysis	Start a gene-set enrichment analysis with this gene as the seed list. Add more genes on that page before running it.		open →