Gene detail

Detailed information of HSymV2.0_g11.19744_t1 in Hydractinia symbiolongicarpus

Genomic Location: HiC_scaffold_11:11206765...11216818
NR annotation: XP_002163629.1, 60S ribosomal protein L14 [Hydra vulgaris]
Species Hydractinia symbiolongicarpus · all data for this species · gene families

Gene Structure

More details in JBrowse

Sequence

CDS

Transcript

Protein

UniProt (Swiss-Prot top hit)

UniProt accession	Description
Q9T043	Large ribosomal subunit protein eL14y OS=Arabidopsis thaliana OX=3702 GN=RPL14B PE=1 SV=1
P55844	Large ribosomal subunit protein eL14 OS=Pisum sativum OX=3888 PE=2 SV=1
Q9SIM4	Large ribosomal subunit protein eL14z OS=Arabidopsis thaliana OX=3702 GN=RPL14A PE=2 SV=1

Gene family

Family type	Membership / link
Orthogroup (gene family)	OG0007171 (this species only) · gene tree & orthology

Pfam domain

Pfam accession	Pfam name	Description	Type	Source
PF01929 all species →	Ribosomal_L14e	Ribosomal protein L14	Family	Interproscan

InterPro

InterPro term	Type	Description	Source
IPR039660 all species →	Family	Large ribosomal subunit protein eL14	Interproscan
IPR002784 all species →	Domain	Large ribosomal subunit protein eL14 domain	Interproscan
IPR041985 all species →	Domain	Large ribosomal subunit protein eL14, KOW motif	Interproscan
IPR014722 all species →	Homologous_superfamily	Large ribosomal subunit protein uL2, domain 2	Interproscan
IPR008991 all species →	Homologous_superfamily	Translation protein SH3-like domain superfamily	Interproscan

PANTHER

PANTHER term	Description	Source
PTHR11127 all species →	60S RIBOSOMAL PROTEIN L14	Interproscan

Gene Ontology

GO term	Category	Description	Source
GO:0003723 all species →	Molecular Function	RNA binding	Interproscan
GO:0003735 all species →	Molecular Function	structural constituent of ribosome	Interproscan
GO:0022625 all species →	Cellular Component	cytosolic large ribosomal subunit	Interproscan
GO:0042273 all species →	Biological Process	ribosomal large subunit biogenesis	Interproscan
GO:0005840 all species →	Cellular Component	ribosome	Interproscan
GO:0006412 all species →	Biological Process	translation	Interproscan

Search by domain instead of by gene. Any accession above (InterPro, Pfam, PANTHER, GO, KEGG) can be used as a query on the Functional Domain Search page, which searches all 148 annotated genomes at once.

KEGG pathway

KO	Enzyme	Enzyme ID	Pathway	Map ID	Source
K02875	RP-L14e, RPL14; large subunit ribosomal protein L14e	-	Ribosome	ko03011	deepkoala

Searching by KO or pathway ID across all species is available on the KEGG Pathway page.

Expression pattern (RNA-seq)

Transcript abundance of HSymV2.0_g11.19744_t1 across 44 RNA-seq samples of Hydractinia symbiolongicarpus. Values are TPM (transcripts per million) from the StringTie quantification; one bar is one sample, grouped and coloured by condition, sorted by expression within each group.

44Samples

44TPM > 0

3Conditions

3,704.3Max TPM

1,056.1Mean TPM

By condition

Condition	Samples	TPM > 0	Mean TPM	Max TPM
Whole embryo	36	36	1,100.75	3,704.30
Whole embryo · Triptolide 20 uM	4	4	810.10	928.58
Whole embryo · DMSO 0.5%	4	4	899.99	1,104.90

Per sample · hover a bar for the full sample record

Show the sample table (44 samples)

SRA run	Condition	Tissue	Developmental stage	Treatment	Study	TPM
SRR24482155	Whole embryo	Whole embryo	72 hpf	not recorded	SRP436676	3,704.30
SRR24482156	Whole embryo	Whole embryo	72 hpf	not recorded	SRP436676	3,658.20
SRR24482158	Whole embryo	Whole embryo	48 hpf	not recorded	SRP436676	3,248.09
SRR24482157	Whole embryo	Whole embryo	48 hpf	not recorded	SRP436676	2,602.51
SRR24482159	Whole embryo	Whole embryo	24 hpf	not recorded	SRP436676	2,512.13
SRR24482160	Whole embryo	Whole embryo	24 hpf	not recorded	SRP436676	2,003.76
SRR24482163	Whole embryo	Whole embryo	6 hpf	not recorded	SRP436676	1,195.35
SRR24482161	Whole embryo	Whole embryo	7 hpf	not recorded	SRP436676	1,082.91
SRR24482162	Whole embryo	Whole embryo	7 hpf	not recorded	SRP436676	1,024.13
SRR24482168	Whole embryo	Whole embryo	3 hpf	not recorded	SRP436676	947.69
SRR24482175	Whole embryo	Whole embryo	Unfertilized egg	not recorded	SRP436676	894.56
SRR24482170	Whole embryo	Whole embryo	2 hpf	not recorded	SRP436676	879.70
SRR24482171	Whole embryo	Whole embryo	1 hpf	not recorded	SRP436676	867.78
SRR24482166	Whole embryo	Whole embryo	4 hpf	not recorded	SRP436676	862.95
SRR24482172	Whole embryo	Whole embryo	30 mpf	not recorded	SRP436676	853.35
SRR24482176	Whole embryo	Whole embryo	1 hpf	not recorded	SRP436676	843.08
SRR24482173	Whole embryo	Whole embryo	Unfertilized egg	not recorded	SRP436676	747.83
SRR24482169	Whole embryo	Whole embryo	2 hpf	not recorded	SRP436676	744.92
SRR24482174	Whole embryo	Whole embryo	Unfertilized egg	not recorded	SRP436676	703.49
SRR24482167	Whole embryo	Whole embryo	3 hpf	not recorded	SRP436676	703.16
SRR24482153	Whole embryo	Whole embryo	7 hpf	not recorded	SRP436676	702.85
SRR24482134	Whole embryo	Whole embryo	5 hpf	not recorded	SRP436676	695.12
SRR24482165	Whole embryo	Whole embryo	4 hpf	not recorded	SRP436676	682.85
SRR24482141	Whole embryo	Whole embryo	1 hpf	not recorded	SRP436676	642.13
SRR24482142	Whole embryo	Whole embryo	30 mpf	not recorded	SRP436676	623.97
SRR24482138	Whole embryo	Whole embryo	3 hpf	not recorded	SRP436676	623.90
SRR24482136	Whole embryo	Whole embryo	4 hpf	not recorded	SRP436676	612.74
SRR24482140	Whole embryo	Whole embryo	2 hpf	not recorded	SRP436676	596.82
SRR24482145	Whole embryo	Whole embryo	Unfertilized egg	not recorded	SRP436676	589.11
SRR24482133	Whole embryo	Whole embryo	6 hpf	not recorded	SRP436676	579.82
SRR24482177	Whole embryo	Whole embryo	1 hpf	not recorded	SRP436676	571.94
SRR24482137	Whole embryo	Whole embryo	3 hpf	not recorded	SRP436676	561.27
SRR24482144	Whole embryo	Whole embryo	Unfertilized egg	not recorded	SRP436676	523.59
SRR24482139	Whole embryo	Whole embryo	2 hpf	not recorded	SRP436676	521.36
SRR24482135	Whole embryo	Whole embryo	4 hpf	not recorded	SRP436676	518.63
SRR24482143	Whole embryo	Whole embryo	Unfertilized egg	not recorded	SRP436676	501.09
SRR24482146	Whole embryo · Triptolide 20 uM	Whole embryo	7 hpf	Triptolide 20 uM	SRP436676	928.58
SRR24482147	Whole embryo · Triptolide 20 uM	Whole embryo	7 hpf	Triptolide 20 uM	SRP436676	927.21
SRR24482150	Whole embryo · Triptolide 20 uM	Whole embryo	7 hpf	Triptolide 20 uM	SRP436676	740.08
SRR24482149	Whole embryo · Triptolide 20 uM	Whole embryo	7 hpf	Triptolide 20 uM	SRP436676	644.55
SRR24482148	Whole embryo · DMSO 0.5%	Whole embryo	7 hpf	DMSO 0.5%	SRP436676	1,104.90
SRR24482154	Whole embryo · DMSO 0.5%	Whole embryo	7 hpf	DMSO 0.5%	SRP436676	1,043.87
SRR24482151	Whole embryo · DMSO 0.5%	Whole embryo	7 hpf	DMSO 0.5%	SRP436676	750.83
SRR24482152	Whole embryo · DMSO 0.5%	Whole embryo	7 hpf	DMSO 0.5%	SRP436676	700.38

Source: CnidoSite RNA-seq expression matrices (HSYMB_TPM, StringTie quantification over 44 runs), joined to SRA sample metadata. Samples whose tissue/treatment is not recorded in the source metadata are grouped by the descriptor carried in the expression matrix itself.

Co-expression network

Genes whose expression across the transcriptome samples of Hydractinia symbiolongicarpus tracks this one. Counts are over the whole network; the network view itself draws at most 100 partners per query gene (CNIDO_NET_TOP_K), so a hub gene can show fewer edges than the number below.

Direction	Partners	Most correlated partner	PCC
Positively correlated	31	HSymV2.0_g12.20878_t1	0.995672428069298
Negatively correlated	35	HSymV2.0_g08.13615_t1	-0.951093916349333

Open this gene in Network Analysis → The network opens with the gene already entered and both directions selected. There you can add up to 9 more genes, switch between the positive and negative network, and export the edge list.

Single-cell expression

Whether this gene can be visualised in the single-cell atlases of Hydractinia symbiolongicarpus, and in which cell types it is a marker. Expression is stored per cell, so the violin plot and the cell-type means are computed in the viewer — open a dataset to see them.

No single-cell dataset has been published for this species. The atlases that do exist are listed in the Cell Atlas.

Epigenetic marks

Chromatin and DNA-methylation data covering this gene. Peak calls are listed per sample with the genomic region the peak falls in; DNA methylation is listed as sample availability only, because those tables are queried by the DNA Methylation page itself.

No epigenomic data has been deposited for this species. The assays that exist across the site are described in the Epigenomic Data module.

What you can do with this gene

Every tool below opens with this gene already entered, so you land on the analysis rather than on an empty form. Links open in a new tab.

Analysis	What it does	Status
Primer design	Design PCR / qPCR primers on this gene’s sequence with the published primer3 settings. Opens with the primers already computed.	template found	open →
BLAST	Search this gene’s sequence against the CnidoSite BLAST databases. The query is filled in and the species’ database is preselected — press Run to start.	template found	open →
Expression heatmap	Draw this gene’s expression as a heatmap across all RNA-seq samples, and compare it with the genes you add next to it.	ready	open →
Gene family / orthogroup	Look this gene up in the single-copy orthogroups built from the high-quality cnidarian genomes, and see which other species carry an orthologue.		open →
Gene set analysis	Start a gene-set enrichment analysis with this gene as the seed list. Add more genes on that page before running it.		open →