Detailed information of HSymV2.0_g12.22129_t1 in Hydractinia symbiolongicarpus

Genomic Location: HiC_scaffold_12:23765869...23776890
NR annotation: XP_047136957.1, autophagy-related protein 9A [Hydra vulgaris]
Species Hydractinia symbiolongicarpus · all data for this species · gene families


 Gene Structure
More details in JBrowse  Sequence
CDS
Transcript
Protein
 UniProt (Swiss-Prot top hit)
UniProt accessionDescription
Q3T904Autophagy-related protein 9A OS=Bos taurus OX=9913 GN=ATG9A PE=2 SV=1
Q5FWU3Autophagy-related protein 9A OS=Rattus norvegicus OX=10116 GN=Atg9a PE=1 SV=1
Q68FE2Autophagy-related protein 9A OS=Mus musculus OX=10090 GN=Atg9a PE=1 SV=2
 Gene family
Family typeMembership / link
Orthogroup (gene family)OG0004353 (this species only) · gene tree & orthology

 Pfam domain
Pfam accessionPfam nameDescriptionTypeSource
PF04109
all species →		ATG9Autophagy protein ATG9FamilyInterproscan

 InterPro
InterPro termTypeDescriptionSource
IPR007241
all species →		FamilyAutophagy-related protein 9Interproscan

 PANTHER
PANTHER termDescriptionSource
PTHR13038
all species →		APG9 AUTOPHAGY 9Interproscan

 Gene Ontology
GO termCategoryDescriptionSource
GO:0000407
all species →		Cellular Componentphagophore assembly siteInterproscan
GO:0000422
all species →		Biological Processautophagy of mitochondrionInterproscan
GO:0005776
all species →		Cellular ComponentautophagosomeInterproscan
GO:0006914
all species →		Biological ProcessautophagyInterproscan
GO:0034497
all species →		Biological Processprotein localization to phagophore assembly siteInterproscan
GO:0044805
all species →		Biological Processobsolete late nucleophagyInterproscan

Search by domain instead of by gene. Any accession above (InterPro, Pfam, PANTHER, GO, KEGG) can be used as a query on the Functional Domain Search page, which searches all 148 annotated genomes at once.
 KEGG pathway
KOEnzymeEnzyme IDPathwayMap IDSource
K17907ATG9; autophagy-related protein 9-Mitochondrial biogenesisko03029deepkoala

Searching by KO or pathway ID across all species is available on the KEGG Pathway page.

Expression pattern (RNA-seq)

Transcript abundance of HSymV2.0_g12.22129_t1 across 44 RNA-seq samples of Hydractinia symbiolongicarpus. Values are TPM (transcripts per million) from the StringTie quantification; one bar is one sample, grouped and coloured by condition, sorted by expression within each group.

44Samples
44TPM > 0
3Conditions
157.1Max TPM
92.9Mean TPM

By condition

ConditionSamplesTPM > 0 Mean TPMMax TPMMean, relative to max
Whole embryo 36 36 84.25 134.93
Whole embryo · Triptolide 20 uM 4 4 134.33 157.11
Whole embryo · DMSO 0.5% 4 4 129.49 140.95

Per sample · hover a bar for the full sample record

Show the sample table (44 samples)
SRA runConditionTissueDevelopmental stage TreatmentStudyTPM
SRR24482153 Whole embryo Whole embryo 7 hpf not recorded SRP436676 134.93
SRR24482162 Whole embryo Whole embryo 7 hpf not recorded SRP436676 131.11
SRR24482161 Whole embryo Whole embryo 7 hpf not recorded SRP436676 127.37
SRR24482165 Whole embryo Whole embryo 4 hpf not recorded SRP436676 124.83
SRR24482163 Whole embryo Whole embryo 6 hpf not recorded SRP436676 118.85
SRR24482133 Whole embryo Whole embryo 6 hpf not recorded SRP436676 115.73
SRR24482166 Whole embryo Whole embryo 4 hpf not recorded SRP436676 110.60
SRR24482167 Whole embryo Whole embryo 3 hpf not recorded SRP436676 106.85
SRR24482139 Whole embryo Whole embryo 2 hpf not recorded SRP436676 103.34
SRR24482134 Whole embryo Whole embryo 5 hpf not recorded SRP436676 99.29
SRR24482144 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 97.42
SRR24482137 Whole embryo Whole embryo 3 hpf not recorded SRP436676 95.64
SRR24482145 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 95.43
SRR24482169 Whole embryo Whole embryo 2 hpf not recorded SRP436676 93.55
SRR24482168 Whole embryo Whole embryo 3 hpf not recorded SRP436676 91.15
SRR24482143 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 89.75
SRR24482136 Whole embryo Whole embryo 4 hpf not recorded SRP436676 88.82
SRR24482140 Whole embryo Whole embryo 2 hpf not recorded SRP436676 88.23
SRR24482138 Whole embryo Whole embryo 3 hpf not recorded SRP436676 87.75
SRR24482135 Whole embryo Whole embryo 4 hpf not recorded SRP436676 87.42
SRR24482141 Whole embryo Whole embryo 1 hpf not recorded SRP436676 86.63
SRR24482142 Whole embryo Whole embryo 30 mpf not recorded SRP436676 85.74
SRR24482177 Whole embryo Whole embryo 1 hpf not recorded SRP436676 84.99
SRR24482170 Whole embryo Whole embryo 2 hpf not recorded SRP436676 77.05
SRR24482173 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 64.29
SRR24482174 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 63.65
SRR24482176 Whole embryo Whole embryo 1 hpf not recorded SRP436676 61.31
SRR24482171 Whole embryo Whole embryo 1 hpf not recorded SRP436676 57.16
SRR24482159 Whole embryo Whole embryo 24 hpf not recorded SRP436676 56.84
SRR24482172 Whole embryo Whole embryo 30 mpf not recorded SRP436676 55.61
SRR24482160 Whole embryo Whole embryo 24 hpf not recorded SRP436676 52.00
SRR24482175 Whole embryo Whole embryo Unfertilized egg not recorded SRP436676 51.82
SRR24482157 Whole embryo Whole embryo 48 hpf not recorded SRP436676 43.01
SRR24482156 Whole embryo Whole embryo 72 hpf not recorded SRP436676 38.14
SRR24482155 Whole embryo Whole embryo 72 hpf not recorded SRP436676 38.07
SRR24482158 Whole embryo Whole embryo 48 hpf not recorded SRP436676 28.50
SRR24482146 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 157.11
SRR24482147 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 151.08
SRR24482150 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 115.56
SRR24482149 Whole embryo · Triptolide 20 uM Whole embryo 7 hpf Triptolide 20 uM SRP436676 113.59
SRR24482154 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 140.95
SRR24482148 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 138.03
SRR24482151 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 123.13
SRR24482152 Whole embryo · DMSO 0.5% Whole embryo 7 hpf DMSO 0.5% SRP436676 115.83

Source: CnidoSite RNA-seq expression matrices (HSYMB_TPM, StringTie quantification over 44 runs), joined to SRA sample metadata. Samples whose tissue/treatment is not recorded in the source metadata are grouped by the descriptor carried in the expression matrix itself.

Co-expression network

Genes whose expression across the transcriptome samples of Hydractinia symbiolongicarpus tracks this one. Counts are over the whole network; the network view itself draws at most 100 partners per query gene (CNIDO_NET_TOP_K), so a hub gene can show fewer edges than the number below.

DirectionPartnersMost correlated partnerPCC
Positively correlated30HSymV2.0_g10.18099_t10.961768783809614
Negatively correlated12HSymV2.0_g11.19743_t1-0.881709785608972

Open this gene in Network Analysis → The network opens with the gene already entered and both directions selected. There you can add up to 9 more genes, switch between the positive and negative network, and export the edge list.

Single-cell expression

Whether this gene can be visualised in the single-cell atlases of Hydractinia symbiolongicarpus, and in which cell types it is a marker. Expression is stored per cell, so the violin plot and the cell-type means are computed in the viewer — open a dataset to see them.

No single-cell dataset has been published for this species. The atlases that do exist are listed in the Cell Atlas.

Epigenetic marks

Chromatin and DNA-methylation data covering this gene. Peak calls are listed per sample with the genomic region the peak falls in; DNA methylation is listed as sample availability only, because those tables are queried by the DNA Methylation page itself.

No epigenomic data has been deposited for this species. The assays that exist across the site are described in the Epigenomic Data module.

What you can do with this gene

Every tool below opens with this gene already entered, so you land on the analysis rather than on an empty form. Links open in a new tab.

AnalysisWhat it doesStatus
Primer designDesign PCR / qPCR primers on this gene’s sequence with the published primer3 settings. Opens with the primers already computed.template foundopen →
BLASTSearch this gene’s sequence against the CnidoSite BLAST databases. The query is filled in and the species’ database is preselected — press Run to start.template foundopen →
Expression heatmapDraw this gene’s expression as a heatmap across all RNA-seq samples, and compare it with the genes you add next to it.readyopen →
Gene family / orthogroupLook this gene up in the single-copy orthogroups built from the high-quality cnidarian genomes, and see which other species carry an orthologue.open →
Gene set analysisStart a gene-set enrichment analysis with this gene as the seed list. Add more genes on that page before running it.open →
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