« All proteomic datasets | Browse the 3673 identified proteins »
| Parameter | As reported by the original study | As applied by CnidoSite |
|---|---|---|
| Search engine | MaxQuant 1.6.14.0 / PD 2.5 SEQUEST HT | Comet 2026.01 + Percolator (Crux 4.2) |
| Sequence database | nveGenes.vienna 2008_02 / UP000001593 | – CnidoSite reference proteome (2.anno), BUSCO-validated |
| Enzyme | – (not restated) | trypsin (fully enzymatic, 2 termini) · up to 2 missed cleavages |
| Precursor tolerance | not stated | 10 ppm |
| Fragment tolerance | not stated | 0.02 Da |
| Fixed modification | – | C+57.021464 (carbamidomethyl cysteine) |
| Variable modification | – | M+15.9949 (methionine oxidation) |
| Decoy strategy | – | Comet internal reversed (1:1) |
| False discovery rate | 0.01 (Percolator) | q ≤ 0.01 at the PSM level (Percolator q-values, target-decoy); protein level q ≤ 0.01 |
| Quantification | – | spectral counting (PSMs) |
| Contaminants | – | cRAP appended and flagged; 17 contaminant proteins identified and excluded from the reported protein list |
| Gene mapping | – | Peptides mapped to CnidoSite gene models; each protein links to its gene page |
| PSMs at q≤0.01 | Peptides at q≤0.01 | Gene-linked proteins | Contaminants (excluded) |
|---|---|---|---|
| 93,156 | 20,212 | 3,673 | 17 |
Pipeline version cnidosite-proteomics 1.0 · CnidoSite release 1.1 · incorporated 2026-09-21
Every stage is a single command; the parameter file written by stage 2 is the one shown in the table above.
| Gene | Protein | Unique peptides | PSMs | Coverage (%) | Length | Best q | Description |
|---|---|---|---|---|---|---|---|
| XP_032239956.1 | NVECT_XP_032239956.1 | 122 | 1648 | 69.9 | 1416 | 0.0000678288 | melanopsin-A [Nematostella vectensis] |
| XP_032229703.1 | NVECT_XP_032229703.1 | 98 | 1011 | 59.2 | 1852 | 0.0000678288 | exocyst complex component 4 isoform X1 [Nematostella vectensis] |
| XP_048582131.1 | NVECT_XP_048582131.1 | 84 | 180 | 20.3 | 5994 | 0.0000678288 | myelin transcription factor 1 isoform X1 [Nematostella vectensis] |
| XP_032219788.2 | NVECT_XP_032219788.2 | 78 | 315 | 33.8 | 3259 | 0.0000678288 | filamin-A isoform X2 [Nematostella vectensis] |
| XP_001640195.2 | NVECT_XP_001640195.2 | 75 | 359 | 15.1 | 4853 | 0.0000678288 | neuronal acetylcholine receptor subunit beta-3 [Nematostella vectensis] |
| XP_001633651.1 | NVECT_XP_001633651.1 | 65 | 710 | 86.1 | 619 | 0.0000678288 | phosphoenolpyruvate carboxykinase, cytosolic [GTP] isoform X2 [Nematostella vectensis] |
| XP_048577779.1 | NVECT_XP_048577779.1 | 59 | 440 | 70.6 | 853 | 0.0000678288 | lysine-specific histone demethylase 1A isoform X2 [Nematostella vectensis] |
| XP_032217949.1 | NVECT_XP_032217949.1 | 56 | 519 | 66.2 | 843 | 0.0000678288 | tubulin polyglutamylase complex subunit 2 isoform X1 [Nematostella vectensis] |
| XP_001629681.1 | NVECT_XP_001629681.1 | 56 | 413 | 65.1 | 723 | 0.0000678288 | 5-formyltetrahydrofolate cyclo-ligase [Nematostella vectensis] |
| XP_032229438.1 | NVECT_XP_032229438.1 | 55 | 123 | 39.8 | 1802 | 0.0000678288 | genetic suppressor element 1 isoform X1 [Nematostella vectensis] |
| XP_032234270.2 | NVECT_XP_032234270.2 | 55 | 94 | 22.1 | 2873 | 0.0000678288 | protein Wnt-4a [Nematostella vectensis] |
| XP_032227699.1 | NVECT_XP_032227699.1 | 48 | 236 | 46.7 | 1323 | 0.0000678288 | polycystic kidney disease protein 1-like 2 [Nematostella vectensis] |
| XP_048584347.1 | NVECT_XP_048584347.1 | 47 | 286 | 49.5 | 1297 | 0.0000678288 | ATP-binding cassette sub-family D member 3 [Nematostella vectensis] |
| XP_048578267.1 | NVECT_XP_048578267.1 | 46 | 255 | 53.6 | 1103 | 0.0000678288 | uncharacterized protein LOC5509100 isoform X3 [Nematostella vectensis] |
| XP_048582392.1 | NVECT_XP_048582392.1 | 46 | 210 | 26.8 | 2251 | 0.0000678288 | CAD protein isoform X2 [Nematostella vectensis] |
