Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD018851

SpeciesMyxobolus honghuensis; Thelohanellus kitauei; Myxobolus wulii
TissueNematocysts (cysts)
PRIDE accessionPXD018851
Statusreprocessed
Search engineComet 2026.01
Reference proteome/mnt/sda/jackie/cnidaria/2.anno/Myxobolus_honghuensis.pep; /mnt/sda/jackie/cnidaria/2.anno/Thelohanellus_kitauei.pep
Enzymetrypsin
Missed cleavages2
Precursor tolerance10 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins1386
Peptides (q≤0.01)4672
PSMs (q≤0.01)6643
reprocessed against the CnidoSite reference proteome
30 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
KII73931.1 TKITA_KII73931.1 1 1 5.3 151 0.000289436 hypothetical protein RF11_05537 [Thelohanellus kitauei]
KII73961.1 TKITA_KII73961.1 1 1 5.3 209 0.000289436 hypothetical protein RF11_14363 [Thelohanellus kitauei]
KII73967.1 TKITA_KII73967.1 1 1 1.1 827 0.0024426 hypothetical protein RF11_14369 [Thelohanellus kitauei]
KII74016.1 TKITA_KII74016.1 1 1 6.5 169 0.000289436 hypothetical protein RF11_04159 [Thelohanellus kitauei]
KII74026.1 TKITA_KII74026.1 1 1 8 137 0.000289436 hypothetical protein RF11_00010 [Thelohanellus kitauei]
KII74214.1 TKITA_KII74214.1 1 1 6.6 151 0.000557569 hypothetical protein RF11_05031 [Thelohanellus kitauei]
KII74426.1 TKITA_KII74426.1 1 1 5.2 230 0.000289436 putative transporter [Thelohanellus kitauei]
KII74594.1 TKITA_KII74594.1 1 1 4.7 641 0.00153571 hypothetical protein RF11_16204 [Thelohanellus kitauei]
KII74945.1 TKITA_KII74945.1 1 1 2.5 324 0.000289436 hypothetical protein RF11_01247 [Thelohanellus kitauei]
KII75020.1 TKITA_KII75020.1 1 1 6.2 193 0.000289436 DNA repair protein RAD51 1 [Thelohanellus kitauei]
KII75062.1 TKITA_KII75062.1 1 1 4.5 289 0.000289436 Vigilin [Thelohanellus kitauei]
Total protein records: 1386  (+30 contaminants hidden)
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