Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD022770

SpeciesCeratonova shasta
TissueDevelopmental stages (in Ascites fluid)
PRIDE accessionPXD022770
Statusreprocessed
Search engineComet 2026.01
Reference proteome/mnt/sdb/jackie/cnidaria_omics/transcriptome_proteins/Ceratonova_shasta.rep.pep
Enzymetrypsin/P
Missed cleavages2
Precursor tolerance10 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949,n+42.010565
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins2239
Peptides (q≤0.01)12107
PSMs (q≤0.01)103880
reprocessed against the CnidoSite reference proteome
41 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
TRINITY_DN3017_c0_g3 TRINITY_DN3017_c0_g3_i11.p1 1 1 11.8 110 0.000124719
TRINITY_DN3070_c0_g1 TRINITY_DN3070_c0_g1_i7.p1 1 1 14.3 133 0.000124719
TRINITY_DN3185_c0_g1 TRINITY_DN3185_c0_g1_i11.p1 1 1 5.9 169 0.00991572
TRINITY_DN3198_c0_g1 TRINITY_DN3198_c0_g1_i2.p1 1 1 6.6 136 0.00826515
TRINITY_DN3283_c0_g1 TRINITY_DN3283_c0_g1_i2.p1 1 1 11.6 121 0.000124719
TRINITY_DN3385_c0_g1 TRINITY_DN3385_c0_g1_i3.p1 1 1 4.3 115 0.0088179
TRINITY_DN3559_c1_g3 TRINITY_DN3559_c1_g3_i4.p1 1 1 11.4 114 0.000124719
TRINITY_DN3714_c0_g1 TRINITY_DN3714_c0_g1_i4.p1 1 1 11.4 105 0.000124719
TRINITY_DN3727_c0_g1 TRINITY_DN3727_c0_g1_i8.p1 1 1 0 111 0.00770648
TRINITY_DN384_c0_g1 TRINITY_DN384_c0_g1_i5.p2 1 1 9.3 118 0.000124719
TRINITY_DN3846_c0_g2 TRINITY_DN3846_c0_g2_i3.p3 1 1 10 130 0.000124719
TRINITY_DN38989_c0_g1 TRINITY_DN38989_c0_g1_i3.p1 1 1 5.7 122 0.00256528
TRINITY_DN3918_c0_g1 TRINITY_DN3918_c0_g1_i11.p1 1 1 6.9 144 0.000124719
TRINITY_DN39239_c0_g1 TRINITY_DN39239_c0_g1_i1.p1 1 1 6.8 146 0.000124719
TRINITY_DN39469_c0_g1 TRINITY_DN39469_c0_g1_i6.p1 1 1 13.1 107 0.000124719
TRINITY_DN39581_c0_g1 TRINITY_DN39581_c0_g1_i1.p1 1 1 5.8 120 0.000220897
TRINITY_DN3991_c0_g1 TRINITY_DN3991_c0_g1_i7.p1 1 1 12.1 140 0.000220897
TRINITY_DN4026_c0_g3 TRINITY_DN4026_c0_g3_i4.p1 1 1 12.5 104 0.000124719
TRINITY_DN4114_c0_g1 TRINITY_DN4114_c0_g1_i8.p1 1 1 5 161 0.00589341
TRINITY_DN4196_c0_g1 TRINITY_DN4196_c0_g1_i2.p2 1 1 8.6 140 0.000124719
TRINITY_DN4201_c0_g3 TRINITY_DN4201_c0_g3_i1.p1 1 1 6.3 111 0.00991572
TRINITY_DN42256_c0_g2 TRINITY_DN42256_c0_g2_i1.p1 1 1 14.3 168 0.00160817
TRINITY_DN43_c0_g1 TRINITY_DN43_c0_g1_i25.p1 1 1 7.8 167 0.000124719
TRINITY_DN4312_c0_g3 TRINITY_DN4312_c0_g3_i2.p1 1 1 5 201 0.00499483
TRINITY_DN438_c0_g1 TRINITY_DN438_c0_g1_i6.p1 1 1 7.5 186 0.000220897
Total protein records: 2239  (+41 contaminants hidden)
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