Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD022770

SpeciesCeratonova shasta
TissueDevelopmental stages (in Ascites fluid)
PRIDE accessionPXD022770
Statusreprocessed
Search engineComet 2026.01
Reference proteome/mnt/sdb/jackie/cnidaria_omics/transcriptome_proteins/Ceratonova_shasta.rep.pep
Enzymetrypsin/P
Missed cleavages2
Precursor tolerance10 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949,n+42.010565
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins2239
Peptides (q≤0.01)12107
PSMs (q≤0.01)103880
reprocessed against the CnidoSite reference proteome
41 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
TRINITY_DN6031_c0_g2 TRINITY_DN6031_c0_g2_i5.p1 1 1 6.7 149 0.00499483
TRINITY_DN6112_c0_g3 TRINITY_DN6112_c0_g3_i3.p1 1 1 11 145 0.000622536
TRINITY_DN6123_c0_g1 TRINITY_DN6123_c0_g1_i12.p1 1 1 12.5 192 0.000124719
TRINITY_DN6219_c1_g1 TRINITY_DN6219_c1_g1_i21.p1 1 1 5.7 157 0.00247706
TRINITY_DN6259_c2_g1 TRINITY_DN6259_c2_g1_i1.p1 1 1 4.3 184 0.000423639
TRINITY_DN633_c0_g1 TRINITY_DN633_c0_g1_i19.p1 1 1 15.6 109 0.000124719
TRINITY_DN6472_c1_g1 TRINITY_DN6472_c1_g1_i3.p1 1 1 8.5 188 0.000124719
TRINITY_DN652_c1_g1 TRINITY_DN652_c1_g1_i2.p1 1 1 10.6 104 0.000124719
TRINITY_DN680_c0_g1 TRINITY_DN680_c0_g1_i10.p1 1 1 8.1 123 0.000124719
TRINITY_DN688_c0_g1 TRINITY_DN688_c0_g1_i4.p1 1 1 5.4 184 0.000124719
TRINITY_DN6906_c1_g1 TRINITY_DN6906_c1_g1_i11.p1 1 1 10.7 131 0.000220897
TRINITY_DN6939_c0_g2 TRINITY_DN6939_c0_g2_i1.p1 1 1 13.1 99 0.000124719
TRINITY_DN7058_c0_g1 TRINITY_DN7058_c0_g1_i7.p1 1 1 8.1 99 0.00221832
TRINITY_DN7298_c0_g1 TRINITY_DN7298_c0_g1_i2.p2 1 1 15.5 110 0.007022
TRINITY_DN746_c0_g3 TRINITY_DN746_c0_g3_i2.p1 1 1 6 133 0.00230415
TRINITY_DN7529_c0_g2 TRINITY_DN7529_c0_g2_i3.p1 1 1 6.8 133 0.00425717
TRINITY_DN7579_c0_g1 TRINITY_DN7579_c0_g1_i3.p1 1 1 8.1 160 0.000124719
TRINITY_DN7637_c0_g1 TRINITY_DN7637_c0_g1_i2.p1 1 1 7.7 104 0.00506394
TRINITY_DN770_c0_g1 TRINITY_DN770_c0_g1_i16.p1 1 1 10 150 0.000124719
TRINITY_DN7722_c0_g3 TRINITY_DN7722_c0_g3_i2.p1 1 1 6.7 149 0.000124719
TRINITY_DN7775_c0_g2 TRINITY_DN7775_c0_g2_i1.p1 1 1 0 114 0.00709459
TRINITY_DN7822_c0_g5 TRINITY_DN7822_c0_g5_i1.p1 1 1 6.1 131 0.00458319
TRINITY_DN7940_c1_g3 TRINITY_DN7940_c1_g3_i1.p1 1 1 8.6 163 0.000220897
TRINITY_DN8073_c0_g1 TRINITY_DN8073_c0_g1_i1.p1 1 1 9.9 101 0.000220897
TRINITY_DN8157_c0_g1 TRINITY_DN8157_c0_g1_i3.p1 1 1 13.5 111 0.000220897
Total protein records: 2239  (+41 contaminants hidden)
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