Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD022770

SpeciesCeratonova shasta
TissueDevelopmental stages (in Ascites fluid)
PRIDE accessionPXD022770
Statusreprocessed
Search engineComet 2026.01
Reference proteome/mnt/sdb/jackie/cnidaria_omics/transcriptome_proteins/Ceratonova_shasta.rep.pep
Enzymetrypsin/P
Missed cleavages2
Precursor tolerance10 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949,n+42.010565
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins2239
Peptides (q≤0.01)12107
PSMs (q≤0.01)103880
reprocessed against the CnidoSite reference proteome
41 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
TRINITY_DN8229_c1_g1 TRINITY_DN8229_c1_g1_i1.p1 1 1 5.4 205 0.000713267
TRINITY_DN8286_c0_g2 TRINITY_DN8286_c0_g2_i1.p1 1 1 9.8 102 0.000976944
TRINITY_DN8296_c0_g2 TRINITY_DN8296_c0_g2_i2.p2 1 1 8.2 147 0.000124719
TRINITY_DN831_c3_g1 TRINITY_DN831_c3_g1_i1.p1 1 1 8.8 114 0.000124719
TRINITY_DN8892_c0_g2 TRINITY_DN8892_c0_g2_i1.p1 1 1 7.5 173 0.00522618
TRINITY_DN9082_c0_g4 TRINITY_DN9082_c0_g4_i6.p1 1 1 5.2 134 0.000220897
TRINITY_DN9181_c0_g1 TRINITY_DN9181_c0_g1_i1.p3 1 1 9.6 125 0.00762399
TRINITY_DN9271_c0_g3 TRINITY_DN9271_c0_g3_i17.p1 1 1 12.2 115 0.00081276
TRINITY_DN9273_c0_g1 TRINITY_DN9273_c0_g1_i2.p1 1 1 6.6 121 0.000423639
TRINITY_DN9371_c0_g1 TRINITY_DN9371_c0_g1_i10.p2 1 1 7.8 102 0.00730663
TRINITY_DN950_c1_g2 TRINITY_DN950_c1_g2_i1.p1 1 1 5.8 103 0.00230415
TRINITY_DN9833_c1_g2 TRINITY_DN9833_c1_g2_i2.p1 1 1 4.1 197 0.00637701
TRINITY_DN9921_c1_g1 TRINITY_DN9921_c1_g1_i12.p2 1 1 9.1 154 0.000220897
TRINITY_DN9926_c0_g1 TRINITY_DN9926_c0_g1_i6.p1 1 1 8.8 102 0.00425717
Total protein records: 2239  (+41 contaminants hidden)
Show: proteins per page
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