Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD030136

SpeciesMyxobolus honghuensis; Thelohanellus kitauei; Myxobolus wulii
TissueNematocysts
PRIDE accessionPXD030136
Statusreprocessed
Search engineComet 2026.01
Reference proteome/mnt/sda/jackie/cnidaria/2.anno/Myxobolus_honghuensis.pep; /mnt/sda/jackie/cnidaria/2.anno/Thelohanellus_kitauei.pep
Enzymetrypsin
Missed cleavages2
Precursor tolerance10 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins1669
Peptides (q≤0.01)6436
PSMs (q≤0.01)20078
reprocessed against the CnidoSite reference proteome
29 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
EVM0005389.1 MHONG_EVM0005389.1 24 13 3.4 4389 0.000254065 gene=EVM0005389
EVM0000059.1 MHONG_EVM0000059.1 82 12 36.3 408 0.000254065 gene=EVM0000059
KII62880.1 TKITA_KII62880.1 43 12 29.3 464 0.000254065 hypothetical protein RF11_12241 [Thelohanellus kitauei]
EVM0001196.1 MHONG_EVM0001196.1 37 12 30.5 433 0.000254065 gene=EVM0001196
EVM0014942.1 MHONG_EVM0014942.1 34 12 29.9 408 0.000254065 gene=EVM0014942
EVM0014716.1 MHONG_EVM0014716.1 28 12 7.4 1276 0.000254065 gene=EVM0014716
EVM0000880.2 MHONG_EVM0000880.2 27 12 31.6 386 0.000254065 gene=EVM0000880
EVM0010658.1 MHONG_EVM0010658.1 27 12 22.5 440 0.000254065 gene=EVM0010658
EVM0014032.1 MHONG_EVM0014032.1 27 12 5.3 2236 0.000254065 gene=EVM0014032
EVM0006176.1 MHONG_EVM0006176.1 26 12 36.5 323 0.000254065 gene=EVM0006176
EVM0001321.1 MHONG_EVM0001321.1 22 12 25.1 538 0.000254065 gene=EVM0001321
EVM0009557.1 MHONG_EVM0009557.1 21 12 36.2 409 0.000254065 gene=EVM0009557
KII73482.1 TKITA_KII73482.1 86 11 31.9 474 0.000254065 hypothetical protein RF11_11811 [Thelohanellus kitauei]
EVM0014928.1 MHONG_EVM0014928.1 66 11 31.4 226 0.000254065 gene=EVM0014928
KII70724.1 TKITA_KII70724.1 65 11 27.5 327 0.000254065 hypothetical protein RF11_09060 [Thelohanellus kitauei]
KII70413.1 TKITA_KII70413.1 58 11 20.3 497 0.000254065 Collagen alpha-3(VI) chain [Thelohanellus kitauei]
EVM0002774.1 MHONG_EVM0002774.1 56 11 7.1 1130 0.000254065 gene=EVM0002774
EVM0006741.1 MHONG_EVM0006741.1 51 11 27.8 381 0.000254065 gene=EVM0006741
KII63674.1 TKITA_KII63674.1 51 11 18.2 674 0.000254065 H/ACA ribonucleoprotein complex subunit 2-like protein [Thelohanellus kitauei]
KII65079.1 TKITA_KII65079.1 48 11 24.2 528 0.000254065 PC3-like endoprotease variant A [Thelohanellus kitauei]
EVM0006375.1 MHONG_EVM0006375.1 39 11 28.9 277 0.000254065 gene=EVM0006375
EVM0012003.1 MHONG_EVM0012003.1 36 11 40.1 247 0.000254065 gene=EVM0012003
EVM0010105.1 MHONG_EVM0010105.1 35 11 39.5 253 0.000254065 gene=EVM0010105
EVM0014780.1 MHONG_EVM0014780.1 33 11 21.6 617 0.000254065 gene=EVM0014780
KII70778.1 TKITA_KII70778.1 32 11 24 529 0.000254065 Endoplasmic reticulum aminopeptidase 1 [Thelohanellus kitauei]
Total protein records: 1669  (+29 contaminants hidden)
Show: proteins per page
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