Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD030136

SpeciesMyxobolus honghuensis; Thelohanellus kitauei; Myxobolus wulii
TissueNematocysts
PRIDE accessionPXD030136
Statusreprocessed
Search engineComet 2026.01
Reference proteome/mnt/sda/jackie/cnidaria/2.anno/Myxobolus_honghuensis.pep; /mnt/sda/jackie/cnidaria/2.anno/Thelohanellus_kitauei.pep
Enzymetrypsin
Missed cleavages2
Precursor tolerance10 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins1669
Peptides (q≤0.01)6436
PSMs (q≤0.01)20078
reprocessed against the CnidoSite reference proteome
29 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
EVM0014864.1 MHONG_EVM0014864.1 1 1 3.1 292 0.00246349 gene=EVM0014864
EVM0014885.1 MHONG_EVM0014885.1 1 1 3.5 396 0.000254065 gene=EVM0014885
EVM0014979.1 MHONG_EVM0014979.1 1 1 2.4 621 0.0098085 gene=EVM0014979
EVM0015195.1 MHONG_EVM0015195.1 1 1 3.1 390 0.000254065 gene=EVM0015195
EVM0015227.1 MHONG_EVM0015227.1 1 1 12.7 126 0.000254065 gene=EVM0015227
EVM0015251.1 MHONG_EVM0015251.1 1 1 3.8 319 0.000981354 gene=EVM0015251
EVM0015304.1 MHONG_EVM0015304.1 1 1 5.9 219 0.0039129 gene=EVM0015304
EVM0015329.1 MHONG_EVM0015329.1 1 1 9.4 171 0.000851426 gene=EVM0015329
EVM0015335.1 MHONG_EVM0015335.1 1 1 4.7 234 0.000851426 gene=EVM0015335
EVM0015364.1 MHONG_EVM0015364.1 1 1 8.6 81 0.00360515 gene=EVM0015364
EVM0015383.1 MHONG_EVM0015383.1 1 1 4.4 297 0.000254065 gene=EVM0015383
EVM0015419.1 MHONG_EVM0015419.1 1 1 1.3 849 0.00881335 gene=EVM0015419
KII61776.1 TKITA_KII61776.1 1 1 3.3 211 0.00909519 hypothetical protein RF11_15846 [Thelohanellus kitauei]
KII62017.1 TKITA_KII62017.1 1 1 4.6 415 0.000851426 hypothetical protein RF11_07045 [Thelohanellus kitauei]
KII62103.1 TKITA_KII62103.1 1 1 8.7 138 0.000254065 Speckle targeted PIP5K1A-regulated poly(A) polymerase [Thelohanellus kitauei]
KII62162.1 TKITA_KII62162.1 1 1 9.8 102 0.0019507 hypothetical protein RF11_07080 [Thelohanellus kitauei]
KII62256.1 TKITA_KII62256.1 1 1 1.1 887 0.000254065 hypothetical protein RF11_10866 [Thelohanellus kitauei]
KII62451.1 TKITA_KII62451.1 1 1 4 303 0.000981354 hypothetical protein RF11_13822 [Thelohanellus kitauei]
KII62681.1 TKITA_KII62681.1 1 1 3.6 337 0.000254065 Mitogen-activated protein kinase 3 [Thelohanellus kitauei]
KII62764.1 TKITA_KII62764.1 1 1 9.2 119 0.000254065 hypothetical protein RF11_08940 [Thelohanellus kitauei]
KII63496.1 TKITA_KII63496.1 1 1 3.9 152 0.00129414 hypothetical protein RF11_01449 [Thelohanellus kitauei]
KII64170.1 TKITA_KII64170.1 1 1 5.2 135 0.0022952 hypothetical protein RF11_03225 [Thelohanellus kitauei]
KII64999.1 TKITA_KII64999.1 1 1 3 298 0.000851426 hypothetical protein RF11_05976 [Thelohanellus kitauei]
KII65036.1 TKITA_KII65036.1 1 1 2.1 608 0.000254065 hypothetical protein RF11_10757 [Thelohanellus kitauei]
KII65080.1 TKITA_KII65080.1 1 1 6.5 169 0.00994406 hypothetical protein RF11_05659 [Thelohanellus kitauei]
Total protein records: 1669  (+29 contaminants hidden)
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