Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD033068

SpeciesNematostella vectensis
TissueEmbryos
PRIDE accessionPXD033068
Statusreprocessed
Search engineComet 2026.01
Reference proteomeCnidoSite reference proteome
Enzymetrypsin
Missed cleavages2
Precursor tolerance10 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins3673
Peptides (q≤0.01)20212
PSMs (q≤0.01)93156
reprocessed against the CnidoSite reference proteome
17 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
XP_032239956.1 NVECT_XP_032239956.1 1648 122 69.9 1416 0.0000678288 melanopsin-A [Nematostella vectensis]
XP_032229703.1 NVECT_XP_032229703.1 1011 98 59.2 1852 0.0000678288 exocyst complex component 4 isoform X1 [Nematostella vectensis]
XP_048582131.1 NVECT_XP_048582131.1 180 84 20.3 5994 0.0000678288 myelin transcription factor 1 isoform X1 [Nematostella vectensis]
XP_032219788.2 NVECT_XP_032219788.2 315 78 33.8 3259 0.0000678288 filamin-A isoform X2 [Nematostella vectensis]
XP_001640195.2 NVECT_XP_001640195.2 359 75 15.1 4853 0.0000678288 neuronal acetylcholine receptor subunit beta-3 [Nematostella vectensis]
XP_001633651.1 NVECT_XP_001633651.1 710 65 86.1 619 0.0000678288 phosphoenolpyruvate carboxykinase, cytosolic [GTP] isoform X2 [Nematostella vectensis]
XP_048577779.1 NVECT_XP_048577779.1 440 59 70.6 853 0.0000678288 lysine-specific histone demethylase 1A isoform X2 [Nematostella vectensis]
XP_032217949.1 NVECT_XP_032217949.1 519 56 66.2 843 0.0000678288 tubulin polyglutamylase complex subunit 2 isoform X1 [Nematostella vectensis]
XP_001629681.1 NVECT_XP_001629681.1 413 56 65.1 723 0.0000678288 5-formyltetrahydrofolate cyclo-ligase [Nematostella vectensis]
XP_032229438.1 NVECT_XP_032229438.1 123 55 39.8 1802 0.0000678288 genetic suppressor element 1 isoform X1 [Nematostella vectensis]
XP_032234270.2 NVECT_XP_032234270.2 94 55 22.1 2873 0.0000678288 protein Wnt-4a [Nematostella vectensis]
XP_032227699.1 NVECT_XP_032227699.1 236 48 46.7 1323 0.0000678288 polycystic kidney disease protein 1-like 2 [Nematostella vectensis]
XP_048584347.1 NVECT_XP_048584347.1 286 47 49.5 1297 0.0000678288 ATP-binding cassette sub-family D member 3 [Nematostella vectensis]
XP_048578267.1 NVECT_XP_048578267.1 255 46 53.6 1103 0.0000678288 uncharacterized protein LOC5509100 isoform X3 [Nematostella vectensis]
XP_048582392.1 NVECT_XP_048582392.1 210 46 26.8 2251 0.0000678288 CAD protein isoform X2 [Nematostella vectensis]
XP_048589323.1 NVECT_XP_048589323.1 201 46 48.7 1205 0.0000678288 leucine--tRNA ligase, cytoplasmic isoform X2 [Nematostella vectensis]
XP_001632260.2 NVECT_XP_001632260.2 319 45 66.7 678 0.0000678288 uncharacterized protein LOC5511879 isoform X1 [Nematostella vectensis]
XP_001639666.3 NVECT_XP_001639666.3 231 45 49.8 878 0.0000678288 uncharacterized protein LOC5519796 [Nematostella vectensis]
XP_001639527.2 NVECT_XP_001639527.2 467 44 74 530 0.0000678288 small nuclear ribonucleoprotein Sm D3 [Nematostella vectensis]
XP_001636292.3 NVECT_XP_001636292.3 243 44 51 1066 0.0000678288 uromodulin [Nematostella vectensis]
XP_001641806.3 NVECT_XP_001641806.3 236 44 54.9 843 0.0000678288 acidic mammalian chitinase [Nematostella vectensis]
XP_001637408.3 NVECT_XP_001637408.3 223 43 50.5 847 0.0000678288 ras-related protein Rab-32 [Nematostella vectensis]
XP_048580694.1 NVECT_XP_048580694.1 179 43 50.6 936 0.0000678288 PI-actitoxin-Avd5a-like [Nematostella vectensis]
XP_048579889.1 NVECT_XP_048579889.1 187 42 30.7 1745 0.0000678288 NACHT domain- and WD repeat-containing protein 1 isoform X2 [Nematostella vectensis]
XP_001632744.2 NVECT_XP_001632744.2 327 40 43.7 1067 0.0000678288 tektin-1 [Nematostella vectensis]
Total protein records: 3673  (+17 contaminants hidden)
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