Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD033068

SpeciesNematostella vectensis
TissueEmbryos
PRIDE accessionPXD033068
Statusreprocessed
Search engineComet 2026.01
Reference proteomeCnidoSite reference proteome
Enzymetrypsin
Missed cleavages2
Precursor tolerance10 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins3673
Peptides (q≤0.01)20212
PSMs (q≤0.01)93156
reprocessed against the CnidoSite reference proteome
17 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
XP_032236646.1 NVECT_XP_032236646.1 100 17 43.9 535 0.0000678288 uncharacterized protein LOC116617779 [Nematostella vectensis]
XP_032230641.2 NVECT_XP_032230641.2 98 17 62.8 317 0.0000678288 GDP-L-fucose synthase [Nematostella vectensis]
XP_048583093.1 NVECT_XP_048583093.1 96 17 41.2 532 0.0000678288 T-complex protein 1 subunit zeta isoform X2 [Nematostella vectensis]
XP_048577730.1 NVECT_XP_048577730.1 95 17 39.1 524 0.0000678288 muscle-specific protein 20 [Nematostella vectensis]
XP_001639404.1 NVECT_XP_001639404.1 92 17 45.8 367 0.0000678288 testis, prostate and placenta-expressed protein isoform X2 [Nematostella vectensis]
XP_001623866.1 NVECT_XP_001623866.1 91 17 58.3 271 0.0000678288 DNA-directed RNA polymerase III subunit RPC10 [Nematostella vectensis]
XP_048578234.1 NVECT_XP_048578234.1 91 17 36.7 592 0.0000678288 ankyrin repeat, PH and SEC7 domain containing protein secG [Nematostella vectensis]
XP_032228885.1 NVECT_XP_032228885.1 89 17 37.6 473 0.0000678288 uncharacterized protein LOC116612350 [Nematostella vectensis]
XP_032240753.2 NVECT_XP_032240753.2 89 17 26.2 684 0.0000678288 serine/threonine kinase-like domain-containing protein STKLD1 isoform X2 [Nematostella vectensis]
XP_032240640.1 NVECT_XP_032240640.1 87 17 59.8 348 0.0000678288 arsenite methyltransferase isoform X1 [Nematostella vectensis]
XP_048582745.1 NVECT_XP_048582745.1 85 17 35 720 0.0000678288 uncharacterized protein LOC5516120 isoform X2 [Nematostella vectensis]
XP_048583862.1 NVECT_XP_048583862.1 81 17 18.8 1042 0.0000678288 uncharacterized protein LOC125563100 [Nematostella vectensis]
XP_001629955.3 NVECT_XP_001629955.3 80 17 46.3 503 0.0000678288 tRNA methyltransferase 10 homolog A [Nematostella vectensis]
XP_001633253.2 NVECT_XP_001633253.2 80 17 64.8 341 0.0000678288 HAUS augmin-like complex subunit 7 isoform X3 [Nematostella vectensis]
XP_048585666.1 NVECT_XP_048585666.1 80 17 18 1200 0.0000678288 pyruvate carboxylase, mitochondrial [Nematostella vectensis]
XP_048587233.1 NVECT_XP_048587233.1 80 17 23.7 809 0.0000678288 tyramine receptor Ser-2 isoform X1 [Nematostella vectensis]
XP_001635821.2 NVECT_XP_001635821.2 77 17 40.5 555 0.0000678288 dnaJ homolog subfamily C member 10 isoform X3 [Nematostella vectensis]
XP_032223114.2 NVECT_XP_032223114.2 76 17 29.6 608 0.0000678288 coronin-7 [Nematostella vectensis]
XP_001627210.2 NVECT_XP_001627210.2 74 17 42.9 480 0.0000678288 uncharacterized protein LOC5506483 [Nematostella vectensis]
XP_048589394.1 NVECT_XP_048589394.1 74 17 34.2 690 0.0000678288 neprilysin-4 [Nematostella vectensis]
XP_001634414.2 NVECT_XP_001634414.2 73 17 56.6 297 0.0000678288 protein ELYS isoform X1 [Nematostella vectensis]
XP_001626073.2 NVECT_XP_001626073.2 72 17 33 503 0.0000678288 pyrroline-5-carboxylate reductase 2 [Nematostella vectensis]
XP_032240514.2 NVECT_XP_032240514.2 68 17 51.4 368 0.0000678288 arsenite methyltransferase [Nematostella vectensis]
XP_048577384.1 NVECT_XP_048577384.1 54 17 16.9 1233 0.0000678288 muscle-specific protein 20 isoform X2 [Nematostella vectensis]
XP_048586932.1 NVECT_XP_048586932.1 54 17 21.3 1260 0.0000678288 uncharacterized protein LOC5501973 isoform X2 [Nematostella vectensis]
Total protein records: 3673  (+17 contaminants hidden)
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