Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD033068

SpeciesNematostella vectensis
TissueEmbryos
PRIDE accessionPXD033068
Statusreprocessed
Search engineComet 2026.01
Reference proteomeCnidoSite reference proteome
Enzymetrypsin
Missed cleavages2
Precursor tolerance10 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins3673
Peptides (q≤0.01)20212
PSMs (q≤0.01)93156
reprocessed against the CnidoSite reference proteome
17 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
XP_048585390.1 NVECT_XP_048585390.1 48 16 18 1174 0.0000678288 uncharacterized protein LOC125568075 [Nematostella vectensis]
XP_048575298.1 NVECT_XP_048575298.1 44 16 25.6 778 0.0000678288 aconitate hydratase, mitochondrial-like isoform X2 [Nematostella vectensis]
XP_032238286.1 NVECT_XP_032238286.1 43 16 41.9 535 0.0000678288 homeobox protein TGIF2 [Nematostella vectensis]
XP_001640174.2 NVECT_XP_001640174.2 22 16 43.4 456 0.0000678288 uncharacterized protein LOC5520341 [Nematostella vectensis]
XP_032219932.1 NVECT_XP_032219932.1 157 15 54.2 358 0.0000678288 adhesive plaque matrix protein 2 [Nematostella vectensis]
XP_032225337.1 NVECT_XP_032225337.1 129 15 54.3 221 0.0000678288 DNA-binding protein HEXBP isoform X2 [Nematostella vectensis]
XP_001639133.1 NVECT_XP_001639133.1 123 15 29.6 304 0.0000678288 pyrroline-5-carboxylate reductase 1, mitochondrial [Nematostella vectensis]
XP_001639977.3 NVECT_XP_001639977.3 117 15 57.2 250 0.0000678288 uncharacterized protein LOC5520200 isoform X1 [Nematostella vectensis]
XP_032228991.1 NVECT_XP_032228991.1 116 15 55.3 374 0.0000678288 probable U3 small nucleolar RNA-associated protein 11 [Nematostella vectensis]
XP_048587226.1 NVECT_XP_048587226.1 108 15 59.2 287 0.0000678288 far upstream element-binding protein 1 isoform X1 [Nematostella vectensis]
XP_001632238.2 NVECT_XP_001632238.2 101 15 69.3 205 0.0000678288 beta-catenin-like protein 1 isoform X1 [Nematostella vectensis]
XP_001636019.2 NVECT_XP_001636019.2 85 15 46.4 429 0.0000678288 succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial [Nematostella vectensis]
XP_032239950.2 NVECT_XP_032239950.2 80 15 65.6 294 0.0000678288 uncharacterized protein LOC116619351 [Nematostella vectensis]
XP_001629333.1 NVECT_XP_001629333.1 78 15 46 428 0.0000678288 ADP-ribosylation factor-like protein 5B [Nematostella vectensis]
XP_032239838.1 NVECT_XP_032239838.1 78 15 64.9 302 0.0000678288 coatomer subunit zeta-1 [Nematostella vectensis]
XP_001638585.1 NVECT_XP_001638585.1 76 15 35.7 499 0.0000678288 riboflavin-binding protein [Nematostella vectensis]
XP_001637467.2 NVECT_XP_001637467.2 71 15 50.6 360 0.0000678288 succinate-semialdehyde dehydrogenase, mitochondrial [Nematostella vectensis]
XP_032223329.2 NVECT_XP_032223329.2 71 15 45.7 322 0.0000678288 adenosine receptor A3 [Nematostella vectensis]
XP_032232816.1 NVECT_XP_032232816.1 69 15 40.1 446 0.0000678288 diaminopimelate decarboxylase isoform X2 [Nematostella vectensis]
XP_032218770.1 NVECT_XP_032218770.1 66 15 32.7 599 0.0000678288 F-box/LRR-repeat protein 5 [Nematostella vectensis]
XP_048577224.1 NVECT_XP_048577224.1 66 15 47.8 391 0.0000678288 glycerol-3-phosphate dehydrogenase 1-like protein isoform X2 [Nematostella vectensis]
XP_001634303.3 NVECT_XP_001634303.3 65 15 31 577 0.0000678288 dickkopf-related protein 3 [Nematostella vectensis]
XP_048582269.1 NVECT_XP_048582269.1 52 15 27.2 637 0.0000678288 polyunsaturated fatty acid 5-lipoxygenase [Nematostella vectensis]
XP_048582316.1 NVECT_XP_048582316.1 52 15 28 693 0.0000678288 uncharacterized protein LOC5508952 isoform X1 [Nematostella vectensis]
XP_048586316.1 NVECT_XP_048586316.1 52 15 33.1 484 0.0000678288 FERM domain-containing protein 8 isoform X2 [Nematostella vectensis]
Total protein records: 3673  (+17 contaminants hidden)
Show: proteins per page
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