Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD033068

SpeciesNematostella vectensis
TissueEmbryos
PRIDE accessionPXD033068
Statusreprocessed
Search engineComet 2026.01
Reference proteomeCnidoSite reference proteome
Enzymetrypsin
Missed cleavages2
Precursor tolerance10 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins3673
Peptides (q≤0.01)20212
PSMs (q≤0.01)93156
reprocessed against the CnidoSite reference proteome
17 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
XP_032220008.2 NVECT_XP_032220008.2 1 1 3.3 521 0.0000678288 F-box only protein 8 [Nematostella vectensis]
XP_032220128.2 NVECT_XP_032220128.2 1 1 0.7 1519 0.00221946 A disintegrin and metalloproteinase with thrombospondin motifs 10 isoform X1 [Nematostella vectensis]
XP_032220444.2 NVECT_XP_032220444.2 1 1 2.1 1065 0.0000678288 ubiquitin-protein ligase E3B isoform X2 [Nematostella vectensis]
XP_032220484.2 NVECT_XP_032220484.2 1 1 1.5 664 0.009193 uncharacterized protein LOC5519706 [Nematostella vectensis]
XP_032220588.2 NVECT_XP_032220588.2 1 1 4.5 311 0.0000678288 FGFR1 oncogene partner 2 homolog isoform X2 [Nematostella vectensis]
XP_032220774.2 NVECT_XP_032220774.2 1 1 4.5 380 0.000428895 uncharacterized protein LOC5520092 isoform X2 [Nematostella vectensis]
XP_032221127.1 NVECT_XP_032221127.1 1 1 1.4 1151 0.0000678288 uncharacterized protein LOC116603668 [Nematostella vectensis]
XP_032221157.2 NVECT_XP_032221157.2 1 1 3 265 0.00181798 melatonin receptor type 1B [Nematostella vectensis]
XP_032221183.1 NVECT_XP_032221183.1 1 1 0.9 1378 0.00612214 uncharacterized protein LOC116603702 [Nematostella vectensis]
XP_032221373.2 NVECT_XP_032221373.2 1 1 0.6 1082 0.00635292 zinc finger SWIM domain-containing protein 5 isoform X2 [Nematostella vectensis]
XP_032221435.2 NVECT_XP_032221435.2 1 1 0.8 1020 0.00377418 phytanoyl-CoA dioxygenase domain-containing protein 1 homolog isoform X1 [Nematostella vectensis]
XP_032221565.2 NVECT_XP_032221565.2 1 1 2.4 885 0.0000678288 probable glycosyltransferase STELLO2 isoform X1 [Nematostella vectensis]
XP_032221597.1 NVECT_XP_032221597.1 1 1 5.4 428 0.0000678288 cAMP-dependent protein kinase type I-alpha regulatory subunit [Nematostella vectensis]
XP_032221685.1 NVECT_XP_032221685.1 1 1 0.2 2917 0.00635292 transport and Golgi organization protein 1 homolog isoform X2 [Nematostella vectensis]
XP_032221696.2 NVECT_XP_032221696.2 1 1 3.4 501 0.0000678288 DNA replication licensing factor mcm5-A [Nematostella vectensis]
XP_032221707.2 NVECT_XP_032221707.2 1 1 6.1 164 0.000319305 transmembrane protein 62 isoform X1 [Nematostella vectensis]
XP_032221868.2 NVECT_XP_032221868.2 1 1 3.6 530 0.0000678288 uncharacterized protein LOC5520971 [Nematostella vectensis]
XP_032222111.1 NVECT_XP_032222111.1 1 1 5.5 272 0.0000678288 sorting nexin-11 isoform X2 [Nematostella vectensis]
XP_032222235.1 NVECT_XP_032222235.1 1 1 2.1 675 0.00237299 class E vacuolar protein-sorting machinery protein HSE1 isoform X1 [Nematostella vectensis]
XP_032222345.1 NVECT_XP_032222345.1 1 1 4.6 305 0.0000678288 fibroblast growth factor receptor 4 isoform X2 [Nematostella vectensis]
XP_032222373.1 NVECT_XP_032222373.1 1 1 1.3 946 0.00125178 zinc finger protein 43 [Nematostella vectensis]
XP_032222398.2 NVECT_XP_032222398.2 1 1 1.4 1090 0.0000678288 uncharacterized protein LOC5521196 [Nematostella vectensis]
XP_032222514.1 NVECT_XP_032222514.1 1 1 1.5 1084 0.00177807 arginine kinase [Nematostella vectensis]
XP_032222552.2 NVECT_XP_032222552.2 1 1 6.3 1042 0.00181798 putative uncharacterized protein DDB_G0290521 [Nematostella vectensis]
XP_032222630.2 NVECT_XP_032222630.2 1 1 2.9 723 0.000373552 high mobility group protein 20A [Nematostella vectensis]
Total protein records: 3673  (+17 contaminants hidden)
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