Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD033068

SpeciesNematostella vectensis
TissueEmbryos
PRIDE accessionPXD033068
Statusreprocessed
Search engineComet 2026.01
Reference proteomeCnidoSite reference proteome
Enzymetrypsin
Missed cleavages2
Precursor tolerance10 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins3673
Peptides (q≤0.01)20212
PSMs (q≤0.01)93156
reprocessed against the CnidoSite reference proteome
17 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
XP_032222676.1 NVECT_XP_032222676.1 1 1 1.6 2199 0.00206758 STE20-like serine/threonine-protein kinase isoform X1 [Nematostella vectensis]
XP_032222679.2 NVECT_XP_032222679.2 1 1 2 498 0.00152276 collectin-12 [Nematostella vectensis]
XP_032222716.2 NVECT_XP_032222716.2 1 1 17.2 116 0.0000678288 cat eye syndrome critical region protein 2 homolog [Nematostella vectensis]
XP_032222739.2 NVECT_XP_032222739.2 1 1 1.1 1151 0.000261421 tripartite motif-containing protein 2 isoform X1 [Nematostella vectensis]
XP_032222776.2 NVECT_XP_032222776.2 1 1 1.1 650 0.00607763 rho GTPase-activating protein 15 isoform X1 [Nematostella vectensis]
XP_032223108.2 NVECT_XP_032223108.2 1 1 9.9 233 0.0000678288 uncharacterized protein LOC116604613 [Nematostella vectensis]
XP_032223162.1 NVECT_XP_032223162.1 1 1 0.6 1231 0.00177807 uncharacterized protein LOC5521907 isoform X2 [Nematostella vectensis]
XP_032223291.2 NVECT_XP_032223291.2 1 1 0.4 1645 0.00635292 apoptotic protease-activating factor 1 [Nematostella vectensis]
XP_032223517.2 NVECT_XP_032223517.2 1 1 2.6 272 0.00347826 microtubule-associated protein RP/EB family member 1 isoform X2 [Nematostella vectensis]
XP_032224386.2 NVECT_XP_032224386.2 1 1 5.2 347 0.0011441 kelch-like protein 20 [Nematostella vectensis]
XP_032224635.1 NVECT_XP_032224635.1 1 1 5 480 0.00834458 uncharacterized protein LOC5504306 isoform X4 [Nematostella vectensis]
XP_032224817.2 NVECT_XP_032224817.2 1 1 2.6 343 0.00237299 histone H4 [Nematostella vectensis]
XP_032225287.2 NVECT_XP_032225287.2 1 1 1.4 977 0.0000678288 uncharacterized protein LOC5496521 [Nematostella vectensis]
XP_032226768.1 NVECT_XP_032226768.1 1 1 10.6 180 0.00168473 alpha-ketoglutarate dehydrogenase component 4 [Nematostella vectensis]
XP_032227094.2 NVECT_XP_032227094.2 1 1 2.5 691 0.0000678288 TBC1 domain family member 23 isoform X2 [Nematostella vectensis]
XP_032227275.2 NVECT_XP_032227275.2 1 1 2.9 822 0.0000678288 uncharacterized protein LOC5503106 isoform X2 [Nematostella vectensis]
XP_032227303.1 NVECT_XP_032227303.1 1 1 1.4 927 0.00480322 high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A isoform X2 [Nematostella vectensis]
XP_032227807.2 NVECT_XP_032227807.2 1 1 1.5 400 0.00460853 uncharacterized protein LOC116611540 [Nematostella vectensis]
XP_032227967.1 NVECT_XP_032227967.1 1 1 3.1 515 0.0000678288 UBX domain-containing protein 7 isoform X2 [Nematostella vectensis]
XP_032228029.2 NVECT_XP_032228029.2 1 1 3.2 216 0.00480322 serine/threonine-protein kinase RIO1 [Nematostella vectensis]
XP_032228095.1 NVECT_XP_032228095.1 1 1 15.4 182 0.0000678288 regulator of G-protein signaling 17 [Nematostella vectensis]
XP_032228131.1 NVECT_XP_032228131.1 1 1 1.3 893 0.00401125 protein MFI [Nematostella vectensis]
XP_032228200.2 NVECT_XP_032228200.2 1 1 1.8 509 0.00110382 tRNA pseudouridine synthase Pus10 isoform X2 [Nematostella vectensis]
XP_032228781.2 NVECT_XP_032228781.2 1 1 8.4 440 0.0000678288 centromere/kinetochore protein zw10 homolog [Nematostella vectensis]
XP_032228829.2 NVECT_XP_032228829.2 1 1 9 210 0.0000678288 E3 ubiquitin-protein ligase TRIM71 [Nematostella vectensis]
Total protein records: 3673  (+17 contaminants hidden)
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