Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD033068

SpeciesNematostella vectensis
TissueEmbryos
PRIDE accessionPXD033068
Statusreprocessed
Search engineComet 2026.01
Reference proteomeCnidoSite reference proteome
Enzymetrypsin
Missed cleavages2
Precursor tolerance10 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins3673
Peptides (q≤0.01)20212
PSMs (q≤0.01)93156
reprocessed against the CnidoSite reference proteome
17 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
XP_032228834.1 NVECT_XP_032228834.1 1 1 4 494 0.0000678288 galactosylceramide sulfotransferase [Nematostella vectensis]
XP_032229010.1 NVECT_XP_032229010.1 1 1 7.4 229 0.000373552 protein PALS1 [Nematostella vectensis]
XP_032229157.1 NVECT_XP_032229157.1 1 1 2.7 701 0.0000678288 serine/threonine-protein kinase PAK 2 isoform X3 [Nematostella vectensis]
XP_032229163.2 NVECT_XP_032229163.2 1 1 1.7 586 0.00635292 uncharacterized protein LOC116612496 [Nematostella vectensis]
XP_032229396.2 NVECT_XP_032229396.2 1 1 0.5 1292 0.00759481 uncharacterized protein LOC5505120 isoform X2 [Nematostella vectensis]
XP_032229506.2 NVECT_XP_032229506.2 1 1 3.5 396 0.0000678288 translin [Nematostella vectensis]
XP_032229613.2 NVECT_XP_032229613.2 1 1 6.3 126 0.00583628 uncharacterized protein LOC5505291 isoform X1 [Nematostella vectensis]
XP_032229709.1 NVECT_XP_032229709.1 1 1 0.5 1895 0.00820164 structural maintenance of chromosomes protein 3 [Nematostella vectensis]
XP_032229722.1 NVECT_XP_032229722.1 1 1 1.5 1204 0.0000678288 phospholipid phosphatase 5 isoform X2 [Nematostella vectensis]
XP_032229965.2 NVECT_XP_032229965.2 1 1 5 382 0.0000678288 tubulin polyglutamylase ttll6 [Nematostella vectensis]
XP_032229996.1 NVECT_XP_032229996.1 1 1 0.5 2752 0.00105245 adenosine receptor A3 [Nematostella vectensis]
XP_032230216.2 NVECT_XP_032230216.2 1 1 2.5 557 0.00100538 rRNA methyltransferase 1, mitochondrial isoform X1 [Nematostella vectensis]
XP_032230519.1 NVECT_XP_032230519.1 1 1 2.5 880 0.00211899 trichohyalin [Nematostella vectensis]
XP_032230529.1 NVECT_XP_032230529.1 1 1 0.4 1856 0.00710865 UDP-galactose translocator [Nematostella vectensis]
XP_032230575.1 NVECT_XP_032230575.1 1 1 1 728 0.00105245 zinc finger CCCH domain-containing protein 18 [Nematostella vectensis]
XP_032230583.2 NVECT_XP_032230583.2 1 1 3 302 0.000132864 nucleoredoxin-like protein 2 [Nematostella vectensis]
XP_032230907.1 NVECT_XP_032230907.1 1 1 11.4 185 0.0000678288 dopamine receptor 2 [Nematostella vectensis]
XP_032230972.2 NVECT_XP_032230972.2 1 1 2.5 789 0.0000678288 NPC intracellular cholesterol transporter 2 [Nematostella vectensis]
XP_032231282.2 NVECT_XP_032231282.2 1 1 3 860 0.00386302 fibrosin-1-like protein isoform X2 [Nematostella vectensis]
XP_032231386.1 NVECT_XP_032231386.1 1 1 6.1 228 0.00450917 octopamine receptor beta-2R [Nematostella vectensis]
XP_032231440.2 NVECT_XP_032231440.2 1 1 1.7 900 0.0000678288 rho guanine nucleotide exchange factor 7 isoform X2 [Nematostella vectensis]
XP_032231485.1 NVECT_XP_032231485.1 1 1 4.9 183 0.00749912 uncharacterized protein K02A2.6 [Nematostella vectensis]
XP_032231503.2 NVECT_XP_032231503.2 1 1 3.8 368 0.0000678288 RNA-binding protein Nova-1 isoform X3 [Nematostella vectensis]
XP_032231599.2 NVECT_XP_032231599.2 1 1 1.5 1855 0.0000678288 uncharacterized threonine-rich GPI-anchored glycoprotein PJ4664.02 isoform X2 [Nematostella vectensis]
XP_032231690.2 NVECT_XP_032231690.2 1 1 3.2 252 0.0000678288 acyl-CoA-binding domain-containing protein 6 isoform X2 [Nematostella vectensis]
Total protein records: 3673  (+17 contaminants hidden)
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