Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD033068

SpeciesNematostella vectensis
TissueEmbryos
PRIDE accessionPXD033068
Statusreprocessed
Search engineComet 2026.01
Reference proteomeCnidoSite reference proteome
Enzymetrypsin
Missed cleavages2
Precursor tolerance10 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins3673
Peptides (q≤0.01)20212
PSMs (q≤0.01)93156
reprocessed against the CnidoSite reference proteome
17 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
XP_032232491.1 NVECT_XP_032232491.1 1 1 0.9 779 0.00368363 E3 ubiquitin-protein ligase TRIM71 [Nematostella vectensis]
XP_032232674.2 NVECT_XP_032232674.2 1 1 6.3 255 0.000607533 uncharacterized protein LOC116615275 [Nematostella vectensis]
XP_032232710.2 NVECT_XP_032232710.2 1 1 7.8 141 0.000319305 uncharacterized protein LOC116615292 [Nematostella vectensis]
XP_032232843.2 NVECT_XP_032232843.2 1 1 1.6 820 0.000428895 protein draper [Nematostella vectensis]
XP_032232897.2 NVECT_XP_032232897.2 1 1 18.4 87 0.0000678288 peroxisomal leader peptide-processing protease-like [Nematostella vectensis]
XP_032233098.2 NVECT_XP_032233098.2 1 1 2.5 285 0.0077317 uncharacterized protein LOC5508448 [Nematostella vectensis]
XP_032233179.1 NVECT_XP_032233179.1 1 1 1.2 1097 0.0000678288 protein C19orf12 homolog [Nematostella vectensis]
XP_032233287.1 NVECT_XP_032233287.1 1 1 3.3 479 0.00186782 probable NADH dehydrogenase [Nematostella vectensis]
XP_032233289.2 NVECT_XP_032233289.2 1 1 1.3 451 0.00985197 uncharacterized protein LOC5508631 isoform X1 [Nematostella vectensis]
XP_032233386.1 NVECT_XP_032233386.1 1 1 12.4 89 0.0000678288 uncharacterized protein LOC116615671 [Nematostella vectensis]
XP_032233878.2 NVECT_XP_032233878.2 1 1 8.1 272 0.0000678288 uncharacterized protein K02A2.6-like [Nematostella vectensis]
XP_032233913.1 NVECT_XP_032233913.1 1 1 2.5 393 0.0000678288 T-box transcription factor T homolog 1 isoform X2 [Nematostella vectensis]
XP_032234291.2 NVECT_XP_032234291.2 1 1 2.4 290 0.00211899 uncharacterized protein LOC116616316 [Nematostella vectensis]
XP_032234476.2 NVECT_XP_032234476.2 1 1 2.5 525 0.0053432 potassium channel subfamily K member 3 [Nematostella vectensis]
XP_032234847.1 NVECT_XP_032234847.1 1 1 1 1058 0.00583628 protein Mis18-alpha isoform X2 [Nematostella vectensis]
XP_032235540.2 NVECT_XP_032235540.2 1 1 1.9 1100 0.0000678288 uncharacterized protein LOC116617183 isoform X1 [Nematostella vectensis]
XP_032235620.2 NVECT_XP_032235620.2 1 1 5.5 329 0.0000678288 melanocyte-stimulating hormone receptor [Nematostella vectensis]
XP_032235664.1 NVECT_XP_032235664.1 1 1 2.5 720 0.0000678288 vitamin K-dependent gamma-carboxylase [Nematostella vectensis]
XP_032235681.1 NVECT_XP_032235681.1 1 1 7.7 324 0.0000678288 neuronal acetylcholine receptor subunit alpha-7 [Nematostella vectensis]
XP_032235934.2 NVECT_XP_032235934.2 1 1 4.3 439 0.00201712 protein piccolo [Nematostella vectensis]
XP_032235984.2 NVECT_XP_032235984.2 1 1 0.3 4356 0.000373552 ranBP-type and C3HC4-type zinc finger-containing protein 1 [Nematostella vectensis]
XP_032236011.2 NVECT_XP_032236011.2 1 1 4 276 0.000132864 serine-arginine protein 55 isoform X2 [Nematostella vectensis]
XP_032236357.2 NVECT_XP_032236357.2 1 1 4.2 307 0.000198623 tissue inhibitor of metalloproteinase [Nematostella vectensis]
XP_032236474.2 NVECT_XP_032236474.2 1 1 3.2 709 0.0000678288 mediator of RNA polymerase II transcription subunit 15 isoform X2 [Nematostella vectensis]
XP_032236787.1 NVECT_XP_032236787.1 1 1 7.4 271 0.0000678288 QRFP-like peptide receptor [Nematostella vectensis]
Total protein records: 3673  (+17 contaminants hidden)
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