Proteomic Analysis

Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants	include cRAP contaminants in the table

Dataset provenance & search parameters — PXD033068

SpeciesNematostella vectensis

TissueEmbryos

PRIDE accessionPXD033068

Statusreprocessed

Search engineComet 2026.01

Reference proteomeCnidoSite reference proteome

Enzymetrypsin

Missed cleavages2

Precursor tolerance10 ppm

Fragment tolerance0.02 Da

Fixed modificationC+57.021464

Variable modificationM+15.9949

Decoy strategyComet internal reversed (1:1)

FDR (PSM)0.01

Quantificationspectral counting (PSMs)

Proteins3673

Peptides (q≤0.01)20212

PSMs (q≤0.01)93156

reprocessed against the CnidoSite reference proteome

17 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.

Gene	Protein	PSMs	Unique peptides	Coverage (%)	Length	Best q-value	Description
XP_001639320.1	NVECT_XP_001639320.1	72	14	34.3	434	0.0000678288	cytochrome b5 [Nematostella vectensis]
XP_001639938.2	NVECT_XP_001639938.2	71	14	40.7	487	0.0000678288	synaptotagmin-14 [Nematostella vectensis]
XP_048578230.1	NVECT_XP_048578230.1	69	14	30.4	611	0.0000678288	NADP-dependent malic enzyme [Nematostella vectensis]
XP_001628105.2	NVECT_XP_001628105.2	64	14	43.6	404	0.0000678288	leucine-rich repeat-containing protein 74A [Nematostella vectensis]
XP_001633665.3	NVECT_XP_001633665.3	64	14	27.3	622	0.0000678288	plasmanylethanolamine desaturase [Nematostella vectensis]
XP_032238524.2	NVECT_XP_032238524.2	63	14	38	324	0.0000678288	metabotropic glutamate receptor 3 isoform X2 [Nematostella vectensis]
XP_001634243.3	NVECT_XP_001634243.3	62	14	58.5	248	0.0000678288	deoxyguanosine kinase isoform X2 [Nematostella vectensis]
XP_001639447.3	NVECT_XP_001639447.3	62	14	40.6	453	0.0000678288	nose resistant to fluoxetine protein 6 isoform X1 [Nematostella vectensis]
XP_048580058.1	NVECT_XP_048580058.1	62	14	28.4	545	0.0000678288	uncharacterized protein LOC5515565 [Nematostella vectensis]
XP_048590316.1	NVECT_XP_048590316.1	62	14	45.4	324	0.0000678288	uncharacterized protein LOC116613064 [Nematostella vectensis]
XP_032220769.2	NVECT_XP_032220769.2	59	14	55.5	400	0.0000678288	translocation protein SEC62 isoform X2 [Nematostella vectensis]
XP_032232937.1	NVECT_XP_032232937.1	59	14	39.4	348	0.0000678288	bublin coiled-coil protein [Nematostella vectensis]
XP_001625725.1	NVECT_XP_001625725.1	58	14	24.5	621	0.0000678288	testis-specific serine/threonine-protein kinase 3 [Nematostella vectensis]
XP_048588502.1	NVECT_XP_048588502.1	58	14	27.4	584	0.0000678288	WAG22 antigen-like [Nematostella vectensis]
XP_001642107.3	NVECT_XP_001642107.3	57	14	43.7	323	0.0000678288	allatostatin-A receptor isoform X2 [Nematostella vectensis]
XP_048589032.1	NVECT_XP_048589032.1	57	14	33.8	480	0.0000678288	argininosuccinate lyase isoform X1 [Nematostella vectensis]
XP_001641644.2	NVECT_XP_001641644.2	56	14	25.3	546	0.0000678288	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 [Nematostella vectensis]
XP_048589942.1	NVECT_XP_048589942.1	53	14	28.4	511	0.0000678288	uncharacterized protein LOC116612677 [Nematostella vectensis]
XP_001634603.1	NVECT_XP_001634603.1	50	14	31.5	448	0.0000678288	probable 28S ribosomal protein S25, mitochondrial [Nematostella vectensis]
XP_001641590.1	NVECT_XP_001641590.1	50	14	40.6	431	0.0000678288	serine protease 23 [Nematostella vectensis]
XP_001635316.2	NVECT_XP_001635316.2	48	14	42.5	515	0.0000678288	uncharacterized protein LOC5515145 [Nematostella vectensis]
XP_032231429.2	NVECT_XP_032231429.2	48	14	17.2	1111	0.0000678288	uncharacterized protein LOC5507066 [Nematostella vectensis]
XP_001628435.2	NVECT_XP_001628435.2	47	14	36.4	418	0.0000678288	follistatin-related protein 1 [Nematostella vectensis]
XP_048576525.1	NVECT_XP_048576525.1	45	14	37.1	485	0.0000678288	regulation of nuclear pre-mRNA domain-containing protein 1B [Nematostella vectensis]
XP_001641259.2	NVECT_XP_001641259.2	44	14	16.3	1158	0.0000678288	type 2 phosphatidylinositol 4,5-bisphosphate 4-phosphatase [Nematostella vectensis]

Total protein records: 3673 (+17 contaminants hidden)

Show: proteins per page