Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD033068

SpeciesNematostella vectensis
TissueEmbryos
PRIDE accessionPXD033068
Statusreprocessed
Search engineComet 2026.01
Reference proteomeCnidoSite reference proteome
Enzymetrypsin
Missed cleavages2
Precursor tolerance10 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins3673
Peptides (q≤0.01)20212
PSMs (q≤0.01)93156
reprocessed against the CnidoSite reference proteome
17 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
XP_032236973.2 NVECT_XP_032236973.2 1 1 0.7 872 0.00308183 uncharacterized protein LOC5511755 isoform X1 [Nematostella vectensis]
XP_032237110.1 NVECT_XP_032237110.1 1 1 4.2 313 0.0000678288 cardiolipin synthase (CMP-forming) isoform X2 [Nematostella vectensis]
XP_032237211.1 NVECT_XP_032237211.1 1 1 4.7 258 0.000428895 uncharacterized transmembrane protein DDB_G0281039 [Nematostella vectensis]
XP_032237224.2 NVECT_XP_032237224.2 1 1 2.3 985 0.00386302 DDB1- and CUL4-associated factor 5 isoform X2 [Nematostella vectensis]
XP_032237244.2 NVECT_XP_032237244.2 1 1 6.4 299 0.00947609 potassium voltage-gated channel subfamily C member 3 isoform X1 [Nematostella vectensis]
XP_032237416.2 NVECT_XP_032237416.2 1 1 0.2 3067 0.00607763 uncharacterized protein LOC5512175 isoform X1 [Nematostella vectensis]
XP_032237422.2 NVECT_XP_032237422.2 1 1 2.5 314 0.00668321 uncharacterized protein LOC116618151 isoform X1 [Nematostella vectensis]
XP_032237680.1 NVECT_XP_032237680.1 1 1 3.9 491 0.00569318 uncharacterized protein LOC116618274 [Nematostella vectensis]
XP_032237699.1 NVECT_XP_032237699.1 1 1 1 1078 0.00211899 intraflagellar transport protein 80 homolog isoform X2 [Nematostella vectensis]
XP_032237828.1 NVECT_XP_032237828.1 1 1 3.3 449 0.0000678288 uncharacterized protein LOC5512555 [Nematostella vectensis]
XP_032237903.2 NVECT_XP_032237903.2 1 1 2.9 447 0.0000678288 adenosine receptor A3-like [Nematostella vectensis]
XP_032238149.1 NVECT_XP_032238149.1 1 1 1.7 1209 0.0000678288 NADH-quinone oxidoreductase subunit B 2 isoform X1 [Nematostella vectensis]
XP_032238177.2 NVECT_XP_032238177.2 1 1 3 493 0.0000678288 centrosomal protein of 57 kDa isoform X2 [Nematostella vectensis]
XP_032238508.2 NVECT_XP_032238508.2 1 1 3.4 417 0.000261421 P2X purinoceptor 4 isoform X2 [Nematostella vectensis]
XP_032238540.2 NVECT_XP_032238540.2 1 1 1.8 380 0.00177807 protein disulfide-isomerase A5 [Nematostella vectensis]
XP_032238599.1 NVECT_XP_032238599.1 1 1 1.1 1232 0.00470406 vitamin K-dependent gamma-carboxylase [Nematostella vectensis]
XP_032238951.2 NVECT_XP_032238951.2 1 1 6.6 286 0.0000678288 protein mab-21-like 2 [Nematostella vectensis]
XP_032238964.2 NVECT_XP_032238964.2 1 1 0.9 1814 0.000607533 transmembrane protein 131 isoform X2 [Nematostella vectensis]
XP_032238993.2 NVECT_XP_032238993.2 1 1 0.9 2092 0.0000678288 uncharacterized protein LOC5513554 isoform X2 [Nematostella vectensis]
XP_032239022.2 NVECT_XP_032239022.2 1 1 0.8 864 0.00298555 uncharacterized protein LOC5513676 [Nematostella vectensis]
XP_032239033.2 NVECT_XP_032239033.2 1 1 5.1 237 0.0000678288 coadhesin [Nematostella vectensis]
XP_032239139.1 NVECT_XP_032239139.1 1 1 2.5 557 0.00668321 organic cation transporter protein [Nematostella vectensis]
XP_032239294.2 NVECT_XP_032239294.2 1 1 2 787 0.000832441 BUD13 homolog [Nematostella vectensis]
XP_032239480.2 NVECT_XP_032239480.2 1 1 2.9 240 0.00177807 5-hydroxytryptamine receptor 7 [Nematostella vectensis]
XP_032239839.1 NVECT_XP_032239839.1 1 1 10.7 177 0.0000678288 homeobox protein unplugged [Nematostella vectensis]
Total protein records: 3673  (+17 contaminants hidden)
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