Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD033068

SpeciesNematostella vectensis
TissueEmbryos
PRIDE accessionPXD033068
Statusreprocessed
Search engineComet 2026.01
Reference proteomeCnidoSite reference proteome
Enzymetrypsin
Missed cleavages2
Precursor tolerance10 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins3673
Peptides (q≤0.01)20212
PSMs (q≤0.01)93156
reprocessed against the CnidoSite reference proteome
17 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
XP_048577782.1 NVECT_XP_048577782.1 1 1 1.1 795 0.000373552 lysine-specific histone demethylase 1A isoform X5 [Nematostella vectensis]
XP_048577804.1 NVECT_XP_048577804.1 1 1 2.2 369 0.00430744 spermine synthase isoform X2 [Nematostella vectensis]
XP_048577825.1 NVECT_XP_048577825.1 1 1 1 1650 0.0000678288 RING finger protein 17 isoform X2 [Nematostella vectensis]
XP_048577858.1 NVECT_XP_048577858.1 1 1 1.8 503 0.000607533 coiled-coil domain-containing protein 81 isoform X1 [Nematostella vectensis]
XP_048577933.1 NVECT_XP_048577933.1 1 1 3.7 543 0.0000678288 transient receptor potential cation channel subfamily A member 1 homolog [Nematostella vectensis]
XP_048577959.1 NVECT_XP_048577959.1 1 1 1.1 625 0.00635292 uncharacterized protein LOC116601524 [Nematostella vectensis]
XP_048578450.1 NVECT_XP_048578450.1 1 1 1.5 1360 0.0026265 DENN domain-containing protein 5B [Nematostella vectensis]
XP_048578521.1 NVECT_XP_048578521.1 1 1 2 949 0.00425797 uncharacterized protein LOC116617360 isoform X1 [Nematostella vectensis]
XP_048578693.1 NVECT_XP_048578693.1 1 1 0.9 910 0.0011441 uncharacterized protein LOC116617362 isoform X1 [Nematostella vectensis]
XP_048578806.1 NVECT_XP_048578806.1 1 1 1.9 375 0.009193 histamine H2 receptor [Nematostella vectensis]
XP_048578978.1 NVECT_XP_048578978.1 1 1 4.7 214 0.00232395 extended synaptotagmin-2 isoform X2 [Nematostella vectensis]
XP_048578990.1 NVECT_XP_048578990.1 1 1 1.4 507 0.00450917 semaphorin-5A isoform X2 [Nematostella vectensis]
XP_048579167.1 NVECT_XP_048579167.1 1 1 0.6 2917 0.0000678288 tyrosine-protein phosphatase non-receptor type 23 isoform X2 [Nematostella vectensis]
XP_048579266.1 NVECT_XP_048579266.1 1 1 0.1 22767 0.000373552 titin isoform X3 [Nematostella vectensis]
XP_048579639.1 NVECT_XP_048579639.1 1 1 1.2 593 0.00877149 growth factor receptor-bound protein 10 isoform X2 [Nematostella vectensis]
XP_048579761.1 NVECT_XP_048579761.1 1 1 3.8 498 0.0000678288 nesprin-1-like isoform X1 [Nematostella vectensis]
XP_048579868.1 NVECT_XP_048579868.1 1 1 0.9 651 0.00569318 vegetative cell wall protein gp1-like [Nematostella vectensis]
XP_048579969.1 NVECT_XP_048579969.1 1 1 8.3 121 0.000373552 zinc finger protein GLI4 [Nematostella vectensis]
XP_048580164.1 NVECT_XP_048580164.1 1 1 9.6 188 0.0000678288 NEDD8-conjugating enzyme UBE2F isoform X2 [Nematostella vectensis]
XP_048580255.1 NVECT_XP_048580255.1 1 1 0.6 1429 0.000132864 collagen alpha chain isoform X2 [Nematostella vectensis]
XP_048580324.1 NVECT_XP_048580324.1 1 1 1 777 0.0000678288 peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase isoform X2 [Nematostella vectensis]
XP_048580512.1 NVECT_XP_048580512.1 1 1 0.1 6789 0.0026265 uncharacterized protein LOC5501855 isoform X2 [Nematostella vectensis]
XP_048580767.1 NVECT_XP_048580767.1 1 1 2.2 364 0.00110382 protein phosphatase 1 regulatory subunit 7 isoform X2 [Nematostella vectensis]
XP_048580852.1 NVECT_XP_048580852.1 1 1 0.4 1496 0.00999406 inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 2 isoform X2 [Nematostella vectensis]
XP_048580931.1 NVECT_XP_048580931.1 1 1 0.6 1016 0.00211899 neuropeptide FF receptor 1-like [Nematostella vectensis]
Total protein records: 3673  (+17 contaminants hidden)
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