Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD033068

SpeciesNematostella vectensis
TissueEmbryos
PRIDE accessionPXD033068
Statusreprocessed
Search engineComet 2026.01
Reference proteomeCnidoSite reference proteome
Enzymetrypsin
Missed cleavages2
Precursor tolerance10 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins3673
Peptides (q≤0.01)20212
PSMs (q≤0.01)93156
reprocessed against the CnidoSite reference proteome
17 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
XP_048583732.1 NVECT_XP_048583732.1 1 1 3.6 274 0.0067243 sorting nexin-27 [Nematostella vectensis]
XP_048583754.1 NVECT_XP_048583754.1 1 1 4.3 325 0.0000678288 metaxin-1 [Nematostella vectensis]
XP_048583869.1 NVECT_XP_048583869.1 1 1 1.2 1103 0.0000678288 insulin gene enhancer protein isl-1 isoform X1 [Nematostella vectensis]
XP_048583933.1 NVECT_XP_048583933.1 1 1 1.5 1272 0.0000678288 uncharacterized protein LOC116602978 [Nematostella vectensis]
XP_048583981.1 NVECT_XP_048583981.1 1 1 2.2 358 0.0077317 snRNA-activating protein complex subunit 3 isoform X2 [Nematostella vectensis]
XP_048584062.1 NVECT_XP_048584062.1 1 1 3.3 396 0.000261421 uncharacterized protein LOC125563171 [Nematostella vectensis]
XP_048584232.1 NVECT_XP_048584232.1 1 1 3.6 450 0.0000678288 kinetochore protein Nuf2-A [Nematostella vectensis]
XP_048584257.1 NVECT_XP_048584257.1 1 1 5.8 260 0.0000678288 atrial natriuretic peptide receptor 1 isoform X1 [Nematostella vectensis]
XP_048584311.1 NVECT_XP_048584311.1 1 1 1.4 915 0.000132864 DNA repair endonuclease XPF isoform X2 [Nematostella vectensis]
XP_048584364.1 NVECT_XP_048584364.1 1 1 7.7 117 0.000832441 spermidine/spermine N(1)-acetyltransferase-like protein 1 [Nematostella vectensis]
XP_048584370.1 NVECT_XP_048584370.1 1 1 0.5 1464 0.0077317 uncharacterized protein LOC125565806 [Nematostella vectensis]
XP_048584524.1 NVECT_XP_048584524.1 1 1 1.4 1754 0.000832441 dedicator of cytokinesis protein 1 isoform X2 [Nematostella vectensis]
XP_048584537.1 NVECT_XP_048584537.1 1 1 0.6 1217 0.00196635 uncharacterized protein LOC5510519 isoform X2 [Nematostella vectensis]
XP_048584541.1 NVECT_XP_048584541.1 1 1 1 884 0.00791108 probable glycoprotein hormone G-protein coupled receptor [Nematostella vectensis]
XP_048584546.1 NVECT_XP_048584546.1 1 1 2.4 802 0.0000678288 delta-1-pyrroline-5-carboxylate synthase isoform X2 [Nematostella vectensis]
XP_048584760.1 NVECT_XP_048584760.1 1 1 0.5 1703 0.00544315 uncharacterized protein LOC116618752 isoform X2 [Nematostella vectensis]
XP_048584846.1 NVECT_XP_048584846.1 1 1 0.2 4570 0.0077317 uncharacterized protein LOC5508594 isoform X1 [Nematostella vectensis]
XP_048584888.1 NVECT_XP_048584888.1 1 1 1.4 1274 0.0000678288 deleted in malignant brain tumors 1 protein-like isoform X1 [Nematostella vectensis]
XP_048584900.1 NVECT_XP_048584900.1 1 1 4 297 0.0000678288 uncharacterized protein K02A2.6-like [Nematostella vectensis]
XP_048584981.1 NVECT_XP_048584981.1 1 1 3 402 0.00157596 E3 ubiquitin-protein ligase RAD18 isoform X2 [Nematostella vectensis]
XP_048584986.1 NVECT_XP_048584986.1 1 1 0.6 1143 0.000319305 polycomb group RING finger protein 6 [Nematostella vectensis]
XP_048585185.1 NVECT_XP_048585185.1 1 1 2.7 255 0.0011441 D-beta-hydroxybutyrate dehydrogenase, mitochondrial [Nematostella vectensis]
XP_048585347.1 NVECT_XP_048585347.1 1 1 2.1 633 0.00956345 condensin-2 complex subunit H2 isoform X3 [Nematostella vectensis]
XP_048585351.1 NVECT_XP_048585351.1 1 1 4.7 296 0.0000678288 alpha-1B adrenergic receptor [Nematostella vectensis]
XP_048585440.1 NVECT_XP_048585440.1 1 1 3.9 440 0.000428895 zinc finger protein ZIC 4 [Nematostella vectensis]
Total protein records: 3673  (+17 contaminants hidden)
Show: proteins per page
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