Proteomic Analysis

Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants	include cRAP contaminants in the table

Dataset provenance & search parameters — PXD033068

SpeciesNematostella vectensis

TissueEmbryos

PRIDE accessionPXD033068

Statusreprocessed

Search engineComet 2026.01

Reference proteomeCnidoSite reference proteome

Enzymetrypsin

Missed cleavages2

Precursor tolerance10 ppm

Fragment tolerance0.02 Da

Fixed modificationC+57.021464

Variable modificationM+15.9949

Decoy strategyComet internal reversed (1:1)

FDR (PSM)0.01

Quantificationspectral counting (PSMs)

Proteins3673

Peptides (q≤0.01)20212

PSMs (q≤0.01)93156

reprocessed against the CnidoSite reference proteome

17 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.

Gene	Protein	PSMs	Unique peptides	Coverage (%)	Length	Best q-value	Description
XP_001637192.3	NVECT_XP_001637192.3	64	13	44.9	428	0.0000678288	radial spoke head 1 homolog [Nematostella vectensis]
XP_001637396.1	NVECT_XP_001637396.1	60	13	74.6	205	0.0000678288	ADP-ribosylation factor-like protein 8B-A [Nematostella vectensis]
XP_001626069.2	NVECT_XP_001626069.2	58	13	31.7	410	0.0000678288	26S proteasome non-ATPase regulatory subunit 3 [Nematostella vectensis]
XP_032226687.2	NVECT_XP_032226687.2	57	13	38.6	417	0.0000678288	uncharacterized protein LOC116609995 [Nematostella vectensis]
XP_001633389.2	NVECT_XP_001633389.2	56	13	35.6	548	0.0000678288	xylose isomerase [Nematostella vectensis]
XP_001640063.2	NVECT_XP_001640063.2	56	13	40.2	358	0.0000678288	uncharacterized protein DDB_G0283697 [Nematostella vectensis]
XP_001634210.3	NVECT_XP_001634210.3	55	13	27.9	448	0.0000678288	retinal homeobox protein Rx1 isoform X1 [Nematostella vectensis]
XP_001637287.3	NVECT_XP_001637287.3	53	13	31.9	439	0.0000678288	neural cell adhesion molecule 1 isoform X2 [Nematostella vectensis]
XP_032241139.1	NVECT_XP_032241139.1	53	13	40.2	361	0.0000678288	uncharacterized protein LOC116619915 isoform X2 [Nematostella vectensis]
XP_001635763.2	NVECT_XP_001635763.2	50	13	30.5	463	0.0000678288	uncharacterized protein LOC5515683 [Nematostella vectensis]
XP_001637088.2	NVECT_XP_001637088.2	50	13	13.3	1120	0.0000678288	pyroglutamylated RF-amide peptide receptor [Nematostella vectensis]
XP_032242056.1	NVECT_XP_032242056.1	49	13	26.3	581	0.0000678288	copine-8 isoform X2 [Nematostella vectensis]
XP_001622977.3	NVECT_XP_001622977.3	48	13	37.3	330	0.0000678288	NADPH-dependent diflavin oxidoreductase 1 isoform X1 [Nematostella vectensis]
XP_001637307.2	NVECT_XP_001637307.2	48	13	25	565	0.0000678288	uncharacterized protein LOC5517227 [Nematostella vectensis]
XP_001634079.2	NVECT_XP_001634079.2	47	13	73.1	242	0.0000678288	snake venom 5'-nucleotidase isoform X1 [Nematostella vectensis]
XP_048575641.1	NVECT_XP_048575641.1	47	13	31.3	406	0.0000678288	uncharacterized protein LOC116611666 isoform X1 [Nematostella vectensis]
XP_048581363.1	NVECT_XP_048581363.1	47	13	42.6	432	0.0000678288	1-aminocyclopropane-1-carboxylate synthase-like protein 1 isoform X2 [Nematostella vectensis]
XP_032240446.2	NVECT_XP_032240446.2	46	13	26.3	547	0.0000678288	ras-related protein Rab-4B isoform X1 [Nematostella vectensis]
XP_001641967.1	NVECT_XP_001641967.1	41	13	42.2	476	0.0000678288	neuropeptide FF receptor 1 [Nematostella vectensis]
XP_048584114.1	NVECT_XP_048584114.1	39	13	25.5	733	0.0000678288	long-chain-fatty-acid--CoA ligase ACSBG2 isoform X2 [Nematostella vectensis]
XP_032232306.1	NVECT_XP_032232306.1	38	13	20.4	744	0.0000678288	myotubularin-related protein 14 [Nematostella vectensis]
XP_032243182.2	NVECT_XP_032243182.2	38	13	18.1	834	0.0000678288	monocarboxylate transporter 9 [Nematostella vectensis]
XP_048575364.1	NVECT_XP_048575364.1	36	13	15.8	1082	0.0000678288	uncharacterized protein LOC116604486 isoform X1 [Nematostella vectensis]
XP_032241561.1	NVECT_XP_032241561.1	23	13	35	411	0.0000678288	protein LEG1 homolog [Nematostella vectensis]
XP_048580085.1	NVECT_XP_048580085.1	23	13	22	764	0.0000678288	la-related protein 4 isoform X2 [Nematostella vectensis]

Total protein records: 3673 (+17 contaminants hidden)

Show: proteins per page