Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD033068

SpeciesNematostella vectensis
TissueEmbryos
PRIDE accessionPXD033068
Statusreprocessed
Search engineComet 2026.01
Reference proteomeCnidoSite reference proteome
Enzymetrypsin
Missed cleavages2
Precursor tolerance10 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins3673
Peptides (q≤0.01)20212
PSMs (q≤0.01)93156
reprocessed against the CnidoSite reference proteome
17 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
XP_032224907.2 NVECT_XP_032224907.2 225 26 55.7 445 0.0000678288 uncharacterized protein LOC116607155 [Nematostella vectensis]
XP_032228771.2 NVECT_XP_032228771.2 169 26 68.5 429 0.0000678288 ESF1 homolog isoform X1 [Nematostella vectensis]
XP_048586951.1 NVECT_XP_048586951.1 164 26 52.5 583 0.0000678288 adenosine receptor A3-like [Nematostella vectensis]
XP_001626440.1 NVECT_XP_001626440.1 159 26 63.9 559 0.0000678288 trans-1,2-dihydrobenzene-1,2-diol dehydrogenase [Nematostella vectensis]
XP_001624954.1 NVECT_XP_001624954.1 141 26 52.1 695 0.0000678288 proteasome subunit beta type-2 [Nematostella vectensis]
XP_032234832.2 NVECT_XP_032234832.2 129 26 36.5 1032 0.0000678288 transcription factor E4F1 [Nematostella vectensis]
XP_048585962.1 NVECT_XP_048585962.1 123 26 52.8 519 0.0000678288 DNA helicase MCM8-like [Nematostella vectensis]
XP_001635835.3 NVECT_XP_001635835.3 120 26 41.1 844 0.0000678288 trichohyalin [Nematostella vectensis]
XP_001639531.2 NVECT_XP_001639531.2 108 26 29.4 1005 0.0000678288 hematopoietic prostaglandin D synthase [Nematostella vectensis]
XP_001633379.2 NVECT_XP_001633379.2 106 26 32.6 972 0.0000678288 putative diacyglycerol O-acyltransferase MT1809 [Nematostella vectensis]
XP_001630130.1 NVECT_XP_001630130.1 350 25 62.7 451 0.0000678288 tubulin alpha-1A chain [Nematostella vectensis]
XP_001641900.2 NVECT_XP_001641900.2 151 25 60.6 426 0.0000678288 ankyrin repeat and MYND domain-containing protein 2 isoform X1 [Nematostella vectensis]
XP_001641743.2 NVECT_XP_001641743.2 135 25 55.8 554 0.0000678288 GATA-binding factor 1-A isoform X7 [Nematostella vectensis]
XP_001628894.1 NVECT_XP_001628894.1 124 25 51.9 516 0.0000678288 rho GDP-dissociation inhibitor 1 [Nematostella vectensis]
XP_032221202.2 NVECT_XP_032221202.2 118 25 45.8 601 0.0000678288 leucine-rich repeat-containing protein 40 isoform X2 [Nematostella vectensis]
XP_032221697.2 NVECT_XP_032221697.2 115 25 40.3 730 0.0000678288 solute carrier family 46 member 3 [Nematostella vectensis]
XP_001635284.3 NVECT_XP_001635284.3 114 25 47.3 660 0.0000678288 DNA-directed RNA polymerase I subunit RPA43 [Nematostella vectensis]
XP_001638124.3 NVECT_XP_001638124.3 100 25 46.8 888 0.0000678288 transcription initiation factor TFIID subunit 9B isoform X1 [Nematostella vectensis]
XP_048583598.1 NVECT_XP_048583598.1 77 25 42.8 725 0.0000678288 myocyte-specific enhancer factor 2C isoform X1 [Nematostella vectensis]
XP_001622280.1 NVECT_XP_001622280.1 194 24 83.3 341 0.0000678288 alpha-1D adrenergic receptor [Nematostella vectensis]
XP_001630400.1 NVECT_XP_001630400.1 189 24 71.2 399 0.0000678288 importin subunit alpha-7 [Nematostella vectensis]
XP_001639808.2 NVECT_XP_001639808.2 180 24 76.4 398 0.0000678288 coiled-coil domain-containing protein 15 [Nematostella vectensis]
XP_001625765.2 NVECT_XP_001625765.2 160 24 49 586 0.0000678288 rho guanine nucleotide exchange factor 12 isoform X17 [Nematostella vectensis]
XP_001629607.1 NVECT_XP_001629607.1 156 24 58 548 0.0000678288 2-hydroxyacyl-CoA lyase 1 [Nematostella vectensis]
XP_001629221.2 NVECT_XP_001629221.2 153 24 61.4 443 0.0000678288 cysteinyl leukotriene receptor 1 [Nematostella vectensis]
Total protein records: 3673  (+17 contaminants hidden)
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