Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD033068

SpeciesNematostella vectensis
TissueEmbryos
PRIDE accessionPXD033068
Statusreprocessed
Search engineComet 2026.01
Reference proteomeCnidoSite reference proteome
Enzymetrypsin
Missed cleavages2
Precursor tolerance10 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins3673
Peptides (q≤0.01)20212
PSMs (q≤0.01)93156
reprocessed against the CnidoSite reference proteome
17 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
XP_032238091.2 NVECT_XP_032238091.2 94 23 59 371 0.0000678288 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related isoform X3 [Nematostella vectensis]
XP_032234199.1 NVECT_XP_032234199.1 89 23 37 587 0.0000678288 3-ketoacyl-CoA thiolase, mitochondrial [Nematostella vectensis]
XP_048581751.1 NVECT_XP_048581751.1 66 23 25.1 1336 0.0000678288 uncharacterized protein LOC5506195 isoform X2 [Nematostella vectensis]
XP_001641860.2 NVECT_XP_001641860.2 269 22 77.9 240 0.0000678288 caseinolytic peptidase B protein homolog isoform X1 [Nematostella vectensis]
XP_032232148.2 NVECT_XP_032232148.2 204 22 69.9 282 0.0000678288 MAM and LDL-receptor class A domain-containing protein 1 [Nematostella vectensis]
XP_001624703.2 NVECT_XP_001624703.2 190 22 53.3 450 0.0000678288 60S ribosomal protein L11 [Nematostella vectensis]
XP_001629500.1 NVECT_XP_001629500.1 161 22 68.4 354 0.0000678288 probable E3 ubiquitin-protein ligase RNF144A-A [Nematostella vectensis]
XP_001629349.2 NVECT_XP_001629349.2 153 22 45 655 0.0000678288 glutamine-rich protein 2 [Nematostella vectensis]
XP_001622550.2 NVECT_XP_001622550.2 135 22 66.2 358 0.0000678288 alanine aminotransferase 2 isoform X2 [Nematostella vectensis]
XP_032230472.1 NVECT_XP_032230472.1 134 22 42.6 645 0.0000678288 nuclear factor NF-kappa-B p100 subunit isoform X2 [Nematostella vectensis]
XP_001639623.2 NVECT_XP_001639623.2 132 22 64.5 428 0.0000678288 protein GUCD1 [Nematostella vectensis]
XP_032232254.1 NVECT_XP_032232254.1 122 22 70.4 392 0.0000678288 neurogenic locus Notch protein isoform X1 [Nematostella vectensis]
XP_032221656.2 NVECT_XP_032221656.2 109 22 23.4 1198 0.0000678288 uncharacterized protein LOC5520759 [Nematostella vectensis]
XP_048588525.1 NVECT_XP_048588525.1 97 22 37.4 721 0.0000678288 propionyl-CoA carboxylase alpha chain, mitochondrial isoform X2 [Nematostella vectensis]
XP_048588552.1 NVECT_XP_048588552.1 97 22 25.5 1002 0.0000678288 chordin-like protein 1 isoform X1 [Nematostella vectensis]
XP_048582906.1 NVECT_XP_048582906.1 95 22 49.6 631 0.0000678288 uncharacterized protein LOC116603391 [Nematostella vectensis]
XP_001632822.1 NVECT_XP_001632822.1 92 22 35.4 725 0.0000678288 spermatogenesis-associated protein 4 [Nematostella vectensis]
XP_001636422.1 NVECT_XP_001636422.1 86 22 49.1 509 0.0000678288 Na(+)/H(+) antiporter NhaA [Nematostella vectensis]
XP_032228742.2 NVECT_XP_032228742.2 79 22 26.9 1055 0.0000678288 alpha-mannosidase 2C1 isoform X2 [Nematostella vectensis]
XP_032228766.2 NVECT_XP_032228766.2 79 22 28.3 1063 0.0000678288 histone deacetylase 6 isoform X1 [Nematostella vectensis]
XP_048589410.1 NVECT_XP_048589410.1 55 22 28.3 1030 0.0000678288 LON peptidase N-terminal domain and RING finger protein 3 [Nematostella vectensis]
XP_032237951.2 NVECT_XP_032237951.2 154 21 64.1 334 0.0000678288 msx2-interacting protein [Nematostella vectensis]
XP_048581487.1 NVECT_XP_048581487.1 150 21 69 484 0.0000678288 methanethiol oxidase-like isoform X2 [Nematostella vectensis]
XP_048578530.1 NVECT_XP_048578530.1 140 21 35.3 496 0.0000678288 uncharacterized protein LOC116610603 [Nematostella vectensis]
XP_032218200.1 NVECT_XP_032218200.1 130 21 41.2 577 0.0000678288 uncharacterized protein LOC116601481 isoform X1 [Nematostella vectensis]
Total protein records: 3673  (+17 contaminants hidden)
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