Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD033068

SpeciesNematostella vectensis
TissueEmbryos
PRIDE accessionPXD033068
Statusreprocessed
Search engineComet 2026.01
Reference proteomeCnidoSite reference proteome
Enzymetrypsin
Missed cleavages2
Precursor tolerance10 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins3673
Peptides (q≤0.01)20212
PSMs (q≤0.01)93156
reprocessed against the CnidoSite reference proteome
17 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
XP_001633282.2 NVECT_XP_001633282.2 155 19 49.8 333 0.0000678288 uncharacterized protein LOC5513019 [Nematostella vectensis]
XP_001642052.1 NVECT_XP_001642052.1 123 19 50.1 443 0.0000678288 actin-like protein 6B [Nematostella vectensis]
XP_001641258.2 NVECT_XP_001641258.2 118 19 54.2 380 0.0000678288 Golgi apparatus protein 1 isoform X2 [Nematostella vectensis]
XP_001636622.2 NVECT_XP_001636622.2 112 19 42.5 572 0.0000678288 putative amidohydrolase YtcJ [Nematostella vectensis]
XP_032240341.2 NVECT_XP_032240341.2 110 19 52.2 425 0.0000678288 transcription factor Sp5 isoform X1 [Nematostella vectensis]
XP_048575932.1 NVECT_XP_048575932.1 107 19 33.3 615 0.0000678288 uncharacterized protein LOC5507411 isoform X1 [Nematostella vectensis]
XP_048590365.1 NVECT_XP_048590365.1 99 19 35.2 619 0.0000678288 uncharacterized skeletal organic matrix protein 7-like isoform X2 [Nematostella vectensis]
XP_001627370.2 NVECT_XP_001627370.2 97 19 64.6 390 0.0000678288 TP53-binding protein 1 isoform X1 [Nematostella vectensis]
XP_048589168.1 NVECT_XP_048589168.1 96 19 22.8 1091 0.0000678288 dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11 isoform X2 [Nematostella vectensis]
XP_001634502.3 NVECT_XP_001634502.3 86 19 50.6 530 0.0000678288 prolyl endopeptidase FAP isoform X1 [Nematostella vectensis]
XP_001622582.1 NVECT_XP_001622582.1 83 19 53.2 496 0.0000678288 eukaryotic translation initiation factor 5A [Nematostella vectensis]
XP_032226358.1 NVECT_XP_032226358.1 81 19 38.7 643 0.0000678288 serine/arginine-rich splicing factor 3 [Nematostella vectensis]
XP_048579854.1 NVECT_XP_048579854.1 79 19 31.8 704 0.0000678288 uncharacterized protein LOC5521725 isoform X2 [Nematostella vectensis]
XP_001630175.2 NVECT_XP_001630175.2 78 19 48.8 449 0.0000678288 esterase OVCA2 isoform X1 [Nematostella vectensis]
XP_048588124.1 NVECT_XP_048588124.1 71 19 27.8 803 0.0000678288 AMP deaminase 2 isoform X2 [Nematostella vectensis]
XP_001636281.2 NVECT_XP_001636281.2 70 19 33.1 625 0.0000678288 meiosis inhibitor protein 1 [Nematostella vectensis]
XP_048585917.1 NVECT_XP_048585917.1 62 19 24.7 845 0.0000678288 glycogen phosphorylase isoform X2 [Nematostella vectensis]
XP_001633828.3 NVECT_XP_001633828.3 60 19 32 519 0.0000678288 protease Do-like 10, mitochondrial [Nematostella vectensis]
XP_001633411.3 NVECT_XP_001633411.3 220 18 46.7 306 0.0000678288 testis-specific serine/threonine-protein kinase 1 [Nematostella vectensis]
XP_032233336.2 NVECT_XP_032233336.2 139 18 60 385 0.0000678288 ras-related protein Rab-38-like [Nematostella vectensis]
XP_001629890.2 NVECT_XP_001629890.2 137 18 52.7 412 0.0000678288 serine/threonine-protein kinase PLK1 [Nematostella vectensis]
XP_001626442.2 NVECT_XP_001626442.2 127 18 55.6 340 0.0000678288 SPARC isoform X2 [Nematostella vectensis]
XP_048585638.1 NVECT_XP_048585638.1 123 18 47.8 406 0.0000678288 neuronal acetylcholine receptor subunit alpha-3 isoform X2 [Nematostella vectensis]
XP_001628190.3 NVECT_XP_001628190.3 120 18 48.2 448 0.0000678288 trifunctional enzyme subunit beta, mitochondrial [Nematostella vectensis]
XP_001632486.1 NVECT_XP_001632486.1 109 18 64.8 352 0.0000678288 putative ammonium transporter 3 [Nematostella vectensis]
Total protein records: 3673  (+17 contaminants hidden)
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