Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD033068

SpeciesNematostella vectensis
TissueEmbryos
PRIDE accessionPXD033068
Statusreprocessed
Search engineComet 2026.01
Reference proteomeCnidoSite reference proteome
Enzymetrypsin
Missed cleavages2
Precursor tolerance10 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins3673
Peptides (q≤0.01)20212
PSMs (q≤0.01)93156
reprocessed against the CnidoSite reference proteome
17 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
XP_001629743.1 NVECT_XP_001629743.1 106 18 62.8 360 0.0000678288 glutathione hydrolase 1 proenzyme [Nematostella vectensis]
XP_001625409.2 NVECT_XP_001625409.2 104 18 41.6 416 0.0000678288 E3 ubiquitin-protein ligase CCNB1IP1 [Nematostella vectensis]
XP_001628191.3 NVECT_XP_001628191.3 97 18 50.2 488 0.0000678288 purine nucleoside phosphorylase LACC1 [Nematostella vectensis]
XP_048584713.1 NVECT_XP_048584713.1 97 18 38.5 480 0.0000678288 uncharacterized protein LOC5521024 isoform X2 [Nematostella vectensis]
XP_048576063.1 NVECT_XP_048576063.1 96 18 35.9 633 0.0000678288 nuclear protein localization protein 4 homolog isoform X2 [Nematostella vectensis]
XP_032233664.2 NVECT_XP_032233664.2 94 18 44.8 525 0.0000678288 transcription intermediary factor 1-beta [Nematostella vectensis]
XP_001639065.3 NVECT_XP_001639065.3 93 18 37.2 537 0.0000678288 uncharacterized protein LOC5519162 [Nematostella vectensis]
XP_001641635.1 NVECT_XP_001641635.1 88 18 39.9 546 0.0000678288 probable methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial isoform X1 [Nematostella vectensis]
XP_001632907.2 NVECT_XP_001632907.2 67 18 51.2 434 0.0000678288 guanine nucleotide-binding protein subunit beta-1 [Nematostella vectensis]
XP_001626726.2 NVECT_XP_001626726.2 65 18 28.4 786 0.0000678288 late histone H2B.L4 [Nematostella vectensis]
XP_001635545.1 NVECT_XP_001635545.1 65 18 48.4 417 0.0000678288 MORN repeat-containing protein 4 [Nematostella vectensis]
XP_048578974.1 NVECT_XP_048578974.1 61 18 24.3 762 0.0000678288 phospholipase D1 [Nematostella vectensis]
XP_048579082.1 NVECT_XP_048579082.1 61 18 18.7 936 0.0000678288 neuropeptide FF receptor 1 [Nematostella vectensis]
XP_048589344.1 NVECT_XP_048589344.1 59 18 17 1251 0.0000678288 dolichyl-phosphate beta-glucosyltransferase [Nematostella vectensis]
XP_001638781.2 NVECT_XP_001638781.2 155 17 71.3 195 0.0000678288 transmembrane protein 242 [Nematostella vectensis]
XP_048582497.1 NVECT_XP_048582497.1 138 17 46.6 384 0.0000678288 ribonucleoside-diphosphate reductase subunit M2 B-like [Nematostella vectensis]
XP_001624805.1 NVECT_XP_001624805.1 129 17 85.6 181 0.0000678288 FUN14 domain-containing protein 1B [Nematostella vectensis]
XP_001633985.2 NVECT_XP_001633985.2 127 17 61.3 225 0.0000678288 actinia tenebrosa protease inhibitors [Nematostella vectensis]
XP_001623059.2 NVECT_XP_001623059.2 121 17 61.5 369 0.0000678288 actin-related protein 2/3 complex subunit 1A-A [Nematostella vectensis]
XP_032237360.2 NVECT_XP_032237360.2 120 17 33.7 656 0.0000678288 plastin-1 isoform X2 [Nematostella vectensis]
XP_001629914.1 NVECT_XP_001629914.1 116 17 48.7 417 0.0000678288 U1 small nuclear ribonucleoprotein C [Nematostella vectensis]
XP_048580735.1 NVECT_XP_048580735.1 116 17 57.7 326 0.0000678288 serine/threonine-protein kinase pakH [Nematostella vectensis]
XP_001624240.3 NVECT_XP_001624240.3 114 17 63 324 0.0000678288 E3 SUMO-protein ligase PIAS2 isoform X1 [Nematostella vectensis]
XP_001626232.1 NVECT_XP_001626232.1 114 17 59.9 446 0.0000678288 nuclear pore complex protein Nup50 [Nematostella vectensis]
XP_001633530.2 NVECT_XP_001633530.2 106 17 47 353 0.0000678288 coronin-1A [Nematostella vectensis]
Total protein records: 3673  (+17 contaminants hidden)
Show: proteins per page
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