Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD041235

SpeciesNematostella vectensis
TissueLarvae/PrimaryPolyps/Juveniles
PRIDE accessionPXD041235
Statusreprocessed
Search engineComet 2026.01
Reference proteomeCnidoSite reference proteome
Enzymenone (endogenous peptides)
Missed cleavages2
Precursor tolerance5 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins385
Peptides (q≤0.01)1393
PSMs (q≤0.01)3147
reprocessed against the CnidoSite reference proteome
1 cRAP contaminant protein  (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
XP_001638852.3 NVECT_XP_001638852.3 1 1 10.4 193 0.00104603 rho-associated protein kinase 1 [Nematostella vectensis]
XP_001639532.3 NVECT_XP_001639532.3 1 1 6.7 208 0.00193986 uncharacterized GTP-binding protein YGR210C [Nematostella vectensis]
XP_001639608.3 NVECT_XP_001639608.3 1 1 4.5 337 0.00339847 N-alpha-acetyltransferase 25, NatB auxiliary subunit isoform X1 [Nematostella vectensis]
XP_001639631.3 NVECT_XP_001639631.3 1 1 23.1 117 0.00104603 rab-like protein 3 [Nematostella vectensis]
XP_001639635.1 NVECT_XP_001639635.1 1 1 6.7 194 0.00104603 neurocalcin homolog isoform X3 [Nematostella vectensis]
XP_001640101.1 NVECT_XP_001640101.1 1 1 3.6 548 0.00104603 QRFP-like peptide receptor [Nematostella vectensis]
XP_001640643.1 NVECT_XP_001640643.1 1 1 0.7 1516 0.00104603 UTP--glucose-1-phosphate uridylyltransferase isoform X1 [Nematostella vectensis]
XP_001641040.2 NVECT_XP_001641040.2 1 1 4.9 472 0.00339847 neurogenin-1 [Nematostella vectensis]
XP_001641146.2 NVECT_XP_001641146.2 1 1 5.7 229 0.00523169 activin receptor type-1 [Nematostella vectensis]
XP_001641381.3 NVECT_XP_001641381.3 1 1 3.4 469 0.00523169 protein SPATA45 homolog [Nematostella vectensis]
XP_001641415.2 NVECT_XP_001641415.2 1 1 20.7 87 0.00104603 kinesin heavy chain isoform X2 [Nematostella vectensis]
XP_001641605.1 NVECT_XP_001641605.1 1 1 15.4 149 0.00104603 checkpoint protein HUS1 [Nematostella vectensis]
XP_032217949.1 NVECT_XP_032217949.1 1 1 1.1 843 0.00865177 tubulin polyglutamylase complex subunit 2 isoform X1 [Nematostella vectensis]
XP_032218257.2 NVECT_XP_032218257.2 1 1 2.6 842 0.00104603 GTP-binding protein 10 isoform X2 [Nematostella vectensis]
XP_032218566.2 NVECT_XP_032218566.2 1 1 0.4 3070 0.00104603 nucleoporin nup211 isoform X2 [Nematostella vectensis]
XP_032219271.2 NVECT_XP_032219271.2 1 1 2.5 1849 0.00339847 uncharacterized protein LOC5518731 isoform X2 [Nematostella vectensis]
XP_032219317.2 NVECT_XP_032219317.2 1 1 3.8 638 0.00104603 myb-related protein B isoform X2 [Nematostella vectensis]
XP_032220357.1 NVECT_XP_032220357.1 1 1 5.5 422 0.00275482 uncharacterized protein LOC116603366 [Nematostella vectensis]
XP_032220905.2 NVECT_XP_032220905.2 1 1 14.1 241 0.00104603 uncharacterized protein LOC116603578 isoform X1 [Nematostella vectensis]
XP_032221127.1 NVECT_XP_032221127.1 1 1 3.2 1151 0.00193986 uncharacterized protein LOC116603668 [Nematostella vectensis]
XP_032221777.1 NVECT_XP_032221777.1 1 1 2.5 560 0.00104603 uncharacterized protein LOC5520745 [Nematostella vectensis]
XP_032226492.2 NVECT_XP_032226492.2 1 1 7.1 198 0.00193986 uncharacterized protein LOC5502339 isoform X2 [Nematostella vectensis]
XP_032226750.1 NVECT_XP_032226750.1 1 1 0.7 1530 0.00808824 ran GTPase-activating protein 1 [Nematostella vectensis]
XP_032228614.2 NVECT_XP_032228614.2 1 1 4 624 0.00193986 polyadenylate-binding protein 4-like isoform X2 [Nematostella vectensis]
XP_032228950.1 NVECT_XP_032228950.1 1 1 1.7 1430 0.00865177 protein still life, isoform SIF type 1 isoform X2 [Nematostella vectensis]
Total protein records: 385  (+1 contaminant hidden)
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