Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD041235

SpeciesNematostella vectensis
TissueLarvae/PrimaryPolyps/Juveniles
PRIDE accessionPXD041235
Statusreprocessed
Search engineComet 2026.01
Reference proteomeCnidoSite reference proteome
Enzymenone (endogenous peptides)
Missed cleavages2
Precursor tolerance5 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins385
Peptides (q≤0.01)1393
PSMs (q≤0.01)3147
reprocessed against the CnidoSite reference proteome
1 cRAP contaminant protein  (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
XP_001630816.1 NVECT_XP_001630816.1 5 3 23.7 135 0.00104603 cystatin-B [Nematostella vectensis]
XP_001637866.3 NVECT_XP_001637866.3 5 3 29 155 0.00104603 GPI inositol-deacylase [Nematostella vectensis]
XP_032231140.2 NVECT_XP_032231140.2 5 3 13.4 232 0.00104603 latrophilin-like protein 1 isoform X4 [Nematostella vectensis]
XP_032238518.2 NVECT_XP_032238518.2 5 3 3.7 1464 0.00104603 enolase-phosphatase E1 isoform X1 [Nematostella vectensis]
XP_048577986.1 NVECT_XP_048577986.1 5 3 4.8 336 0.00104603 solute carrier organic anion transporter family member 4A1 isoform X1 [Nematostella vectensis]
XP_048578661.1 NVECT_XP_048578661.1 5 3 31.2 128 0.00104603 grixazone synthase [Nematostella vectensis]
XP_001622053.2 NVECT_XP_001622053.2 4 3 4.9 426 0.00104603 adenosine receptor A2a [Nematostella vectensis]
XP_001627691.1 NVECT_XP_001627691.1 4 3 6.3 206 0.00104603 uncharacterized protein LOC5506997 [Nematostella vectensis]
XP_001629302.1 NVECT_XP_001629302.1 4 3 14.5 242 0.00104603 maspardin [Nematostella vectensis]
XP_001630070.2 NVECT_XP_001630070.2 4 3 14.1 375 0.00104603 histone H4 [Nematostella vectensis]
XP_001634398.3 NVECT_XP_001634398.3 4 3 17.9 263 0.00104603 interferon-inducible double-stranded RNA-dependent protein kinase activator A homolog A [Nematostella vectensis]
XP_001636960.1 NVECT_XP_001636960.1 4 3 1.7 1952 0.00104603 matrix-remodeling-associated protein 7 [Nematostella vectensis]
XP_001638531.1 NVECT_XP_001638531.1 4 3 8 325 0.00104603 MIP18 family protein galla-2 [Nematostella vectensis]
XP_001639133.1 NVECT_XP_001639133.1 4 3 8.2 304 0.00104603 pyrroline-5-carboxylate reductase 1, mitochondrial [Nematostella vectensis]
XP_032219406.2 NVECT_XP_032219406.2 4 3 11.9 168 0.00104603 uncharacterized protein LOC116602233 [Nematostella vectensis]
XP_032231044.2 NVECT_XP_032231044.2 4 3 16.3 92 0.00104603 LOW QUALITY PROTEIN: myb-like protein Q [Nematostella vectensis]
XP_048582534.1 NVECT_XP_048582534.1 4 3 4.4 939 0.00104603 uncharacterized protein LOC125561875 [Nematostella vectensis]
XP_048585522.1 NVECT_XP_048585522.1 4 3 7.5 388 0.00104603 uncharacterized protein LOC116616799 [Nematostella vectensis]
XP_001634243.3 NVECT_XP_001634243.3 3 3 28.2 248 0.00193986 deoxyguanosine kinase isoform X2 [Nematostella vectensis]
XP_001637478.3 NVECT_XP_001637478.3 3 3 30.2 116 0.00104603 ATP-binding cassette sub-family C member 4 isoform X1 [Nematostella vectensis]
XP_001639742.1 NVECT_XP_001639742.1 3 3 34.8 178 0.00104603 circadian locomoter output cycles protein kaput [Nematostella vectensis]
XP_001639814.1 NVECT_XP_001639814.1 3 3 1.7 875 0.00104603 alkaline ceramidase [Nematostella vectensis]
XP_032220971.2 NVECT_XP_032220971.2 3 3 4.9 1604 0.00104603 trichohyalin isoform X2 [Nematostella vectensis]
XP_032221034.2 NVECT_XP_032221034.2 3 3 7 386 0.00104603 neuropeptides B/W receptor type 2 [Nematostella vectensis]
XP_032227716.2 NVECT_XP_032227716.2 3 3 1.2 2565 0.00104603 uncharacterized protein LOC5503466 isoform X2 [Nematostella vectensis]
Total protein records: 385  (+1 contaminant hidden)
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