Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD060643

SpeciesActinia fragacea
Tissuenematocysts, various tissues
PRIDE accessionPXD060643
Statusreprocessed
Search engineComet 2026.01
Reference proteome/mnt/sdb/jackie/cnidaria_omics/transcriptome_proteins/Actinia_tenebrosa.rep.pep
Enzymetrypsin
Missed cleavages2
Precursor tolerance10 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins4572
Peptides (q≤0.01)24717
PSMs (q≤0.01)130483
reprocessed against the CnidoSite reference proteome
15 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
TRINITY_DN1568_c0_g1 TRINITY_DN1568_c0_g1_i1.p1 24 18 21 1080 0.0000713063
TRINITY_DN7408_c0_g1 TRINITY_DN7408_c0_g1_i3.p1 216 17 66.8 193 0.0000713063
TRINITY_DN1194_c0_g2 TRINITY_DN1194_c0_g2_i6.p1 180 17 23.4 666 0.0000713063
TRINITY_DN31941_c0_g1 TRINITY_DN31941_c0_g1_i1.p1 168 17 33.7 368 0.0000713063
TRINITY_DN3306_c0_g1 TRINITY_DN3306_c0_g1_i1.p1 167 17 52.6 350 0.0000713063
TRINITY_DN4420_c0_g2 TRINITY_DN4420_c0_g2_i11.p1 115 17 26.5 592 0.0000713063
TRINITY_DN2848_c0_g2 TRINITY_DN2848_c0_g2_i1.p1 110 17 34.2 503 0.0000713063
TRINITY_DN191_c1_g3 TRINITY_DN191_c1_g3_i1.p1 106 17 17.6 994 0.0000713063
TRINITY_DN7859_c0_g1 TRINITY_DN7859_c0_g1_i2.p1 101 17 59.3 322 0.0000713063
TRINITY_DN50891_c0_g1 TRINITY_DN50891_c0_g1_i1.p1 99 17 26.9 494 0.0000713063
TRINITY_DN48800_c0_g1 TRINITY_DN48800_c0_g1_i1.p1 94 17 41 600 0.0000713063
TRINITY_DN45475_c0_g1 TRINITY_DN45475_c0_g1_i1.p1 91 17 71.3 171 0.0000713063
TRINITY_DN12065_c0_g1 TRINITY_DN12065_c0_g1_i3.p1 84 17 54.3 289 0.0000713063
TRINITY_DN1640_c5_g1 TRINITY_DN1640_c5_g1_i1.p1 80 17 31.7 628 0.0000713063
TRINITY_DN31205_c0_g1 TRINITY_DN31205_c0_g1_i1.p1 79 17 35.5 485 0.0000713063
TRINITY_DN5446_c1_g2 TRINITY_DN5446_c1_g2_i2.p1 79 17 57.1 340 0.0000713063
TRINITY_DN22899_c0_g1 TRINITY_DN22899_c0_g1_i1.p1 78 17 43.1 422 0.0000713063
TRINITY_DN1443_c3_g1 TRINITY_DN1443_c3_g1_i2.p1 77 17 48 354 0.0000713063
TRINITY_DN577_c8_g1 TRINITY_DN577_c8_g1_i1.p1 77 17 41 524 0.0000713063
TRINITY_DN13854_c0_g1 TRINITY_DN13854_c0_g1_i1.p1 73 17 22.9 853 0.0000713063
TRINITY_DN35245_c0_g1 TRINITY_DN35245_c0_g1_i1.p1 72 17 18.1 1091 0.0000713063
TRINITY_DN310_c3_g1 TRINITY_DN310_c3_g1_i4.p1 64 17 30.3 636 0.0000713063
TRINITY_DN20918_c0_g1 TRINITY_DN20918_c0_g1_i1.p1 56 17 28.5 738 0.0000713063
TRINITY_DN6858_c0_g1 TRINITY_DN6858_c0_g1_i1.p1 46 17 15.4 1079 0.0000713063
TRINITY_DN3139_c1_g1 TRINITY_DN3139_c1_g1_i1.p1 44 17 44.1 479 0.0000713063
Total protein records: 4572  (+15 contaminants hidden)
Show: proteins per page
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