Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD060643

SpeciesActinia fragacea
Tissuenematocysts, various tissues
PRIDE accessionPXD060643
Statusreprocessed
Search engineComet 2026.01
Reference proteome/mnt/sdb/jackie/cnidaria_omics/transcriptome_proteins/Actinia_tenebrosa.rep.pep
Enzymetrypsin
Missed cleavages2
Precursor tolerance10 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins4572
Peptides (q≤0.01)24717
PSMs (q≤0.01)130483
reprocessed against the CnidoSite reference proteome
15 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
TRINITY_DN5570_c1_g1 TRINITY_DN5570_c1_g1_i1.p1 1 1 4.6 262 0.00454295
TRINITY_DN55746_c0_g1 TRINITY_DN55746_c0_g1_i1.p1 1 1 10.2 147 0.000262312
TRINITY_DN55793_c0_g1 TRINITY_DN55793_c0_g1_i1.p1 1 1 8.1 111 0.000359389
TRINITY_DN5580_c0_g1 TRINITY_DN5580_c0_g1_i4.p1 1 1 9.4 117 0.00724817
TRINITY_DN559_c0_g1 TRINITY_DN559_c0_g1_i1.p1 1 1 7.5 174 0.00164551
TRINITY_DN55990_c0_g1 TRINITY_DN55990_c0_g1_i1.p1 1 1 4.4 275 0.0000713063
TRINITY_DN56258_c0_g1 TRINITY_DN56258_c0_g1_i1.p1 1 1 5.1 254 0.00339958
TRINITY_DN56259_c0_g1 TRINITY_DN56259_c0_g1_i1.p1 1 1 6 184 0.00964813
TRINITY_DN56272_c0_g1 TRINITY_DN56272_c0_g1_i1.p1 1 1 6.1 261 0.00300606
TRINITY_DN56551_c0_g1 TRINITY_DN56551_c0_g1_i1.p1 1 1 1.4 842 0.0000713063
TRINITY_DN566_c0_g1 TRINITY_DN566_c0_g1_i12.p1 1 1 1 919 0.00068102
TRINITY_DN56635_c0_g1 TRINITY_DN56635_c0_g1_i1.p1 1 1 8.6 151 0.00126941
TRINITY_DN56814_c0_g1 TRINITY_DN56814_c0_g1_i1.p1 1 1 7.1 226 0.0000713063
TRINITY_DN5684_c2_g1 TRINITY_DN5684_c2_g1_i1.p1 1 1 2.5 878 0.0000713063
TRINITY_DN5685_c0_g1 TRINITY_DN5685_c0_g1_i1.p1 1 1 4.4 204 0.003324
TRINITY_DN5690_c0_g1 TRINITY_DN5690_c0_g1_i1.p1 1 1 0.6 1616 0.000967266
TRINITY_DN56916_c0_g1 TRINITY_DN56916_c0_g1_i1.p1 1 1 6.8 147 0.0000713063
TRINITY_DN56983_c0_g1 TRINITY_DN56983_c0_g1_i1.p1 1 1 1.5 476 0.00546862
TRINITY_DN56994_c0_g1 TRINITY_DN56994_c0_g1_i1.p1 1 1 5.7 106 0.00470498
TRINITY_DN57035_c0_g1 TRINITY_DN57035_c0_g1_i1.p1 1 1 9.9 182 0.0000713063
TRINITY_DN57099_c0_g1 TRINITY_DN57099_c0_g1_i1.p1 1 1 11.7 120 0.0000713063
TRINITY_DN5716_c0_g1 TRINITY_DN5716_c0_g1_i1.p1 1 1 7.9 101 0.00506415
TRINITY_DN57270_c0_g1 TRINITY_DN57270_c0_g1_i1.p1 1 1 1.1 1059 0.00267989
TRINITY_DN5739_c0_g1 TRINITY_DN5739_c0_g1_i5.p1 1 1 11 109 0.00030849
TRINITY_DN5754_c0_g4 TRINITY_DN5754_c0_g4_i1.p1 1 1 1.4 621 0.00347887
Total protein records: 4572  (+15 contaminants hidden)
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